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The map label for this gene is yrbE [H]

Identifier: 50841949

GI number: 50841949

Start: 513559

End: 514770

Strand: Direct

Name: yrbE [H]

Synonym: PPA0466

Alternate gene names: 50841949

Gene position: 513559-514770 (Clockwise)

Preceding gene: 50841948

Following gene: 50841950

Centisome position: 20.06

GC content: 58.33

Gene sequence:

>1212_bases
ATGGGGGTAGTGATGGAGAAGAGACGAACCATTGGTGTCGGCGTGGTTTCGCTGGGATGGATGGGGAGGCTGCACACTCG
TGCGTACAAAGCACTTGCCGAAAAGTTTCCAGAGATTGACGTAAATATCCGACTGGTTTCGTGTTGCGATGTGGTAGCGG
AAAACCGAAGGCAAGCAGTGGACCGGCTGGGATTCTGCACTGCGGTTGAGGACTACCACGATCTCATCGGCAATCCCGAA
GTAGATGTCGTCTCCATCTGCGCGCCAAATTTCCTGCATCGCGATATCGCTCTTGCCGCTGCGGAGGTGGGAAAGCCGTT
CTGGATTGAGAAGCCGATGGGGACTAACGCGAAGCAGTCCCGCCAAATCGCTGAAGCAGCAGAGGCCAAGGGGCTCGTTA
CCTCGGTGGGATTCAACTATCGGCACACTCCCTCTATCGAGTTCGCACGCCAGCTGATAGCTGATGGTCGCCTGGGGACG
GTGACCAATGCCAGGGCATGGCTCATCGCGGACTACGCGTCTGATCCGCACGGGCCACTGACGTGGCGTTATGACAGGGA
GCGGGCCGGTGCCGGCGTCATCCTCGATCTCATGGGTCACGGAGAGGATCTCGTCCAGTATCTACTCAAAGGGAGATTCA
CTGAGGTGTCCGCTGTGTCCGAGACGTTCATCCGTCAGCGTCCCAAGCCACTCAAGGAGGGCATCGGCCACACAGGTTGG
GTCGTCTCGGACGAGCTCGGGCCGGTGGGCAACGAGGATTATTGCGCTGTCATCGCCCGTATGGAAAACGGAGTGATGTG
CACCCTGGAGTCCAGTCGGGTCAGTGTTGGGCCGCGCGCGGAGTACATCGTCGAGATCTATGGAACCGACGGATCAATCC
GGTGGAACTTCGAGGATCTCAACCATTTGCAGGTCTGTCTGGGGCGAAACAATCGTGCCCTGCAGGGATATGTCAACTGC
ATGGCCGGACCAGACTTCCCGGAGTTCATGCGTTTCCAACCGGGAGCCGGAACATCCATGGGCTTCGACGACATGAAGGT
CGTTGAGGCTGCGAAATTCGTCCGAGGGGTCTTGGATGGGCAGCAATATGGCCCATCTGTCGCCGATGGTTGGGCCTCAG
CGGAGGTCAATGACGCGATCGTTGCCTCCTGTGGGGACCATGCCTGGCATGACGTGAAGCCGGTTTCGGGGAGAACCACG
TTCGATAAGTGA

Upstream 100 bases:

>100_bases
CTAAGAGGGTGGACAAGGGCACTCGGTGTGCTATCTTTTTGACAGGACAAATGCACAGGGTTGTGCGTAGGTCCGTCGGC
TATGGGAGCTGGCGCGATTC

Downstream 100 bases:

>100_bases
CCGCGTCATCGCGTGTCTGTGACCAGGCCTGGCGGCACAACCAGGTCGCCGGTGGGAACTTCCGCCGGATGGACTGGGGC
GGGAGCTGCCCCTGACTTGA

Product: putative myo-inositol 2-dehydrogenase

Products: 2,4,6/3,5-pentahydroxycyclohexanone; NADH; H+

Alternate protein names: NA

Number of amino acids: Translated: 403; Mature: 402

Protein sequence:

>403_residues
MGVVMEKRRTIGVGVVSLGWMGRLHTRAYKALAEKFPEIDVNIRLVSCCDVVAENRRQAVDRLGFCTAVEDYHDLIGNPE
VDVVSICAPNFLHRDIALAAAEVGKPFWIEKPMGTNAKQSRQIAEAAEAKGLVTSVGFNYRHTPSIEFARQLIADGRLGT
VTNARAWLIADYASDPHGPLTWRYDRERAGAGVILDLMGHGEDLVQYLLKGRFTEVSAVSETFIRQRPKPLKEGIGHTGW
VVSDELGPVGNEDYCAVIARMENGVMCTLESSRVSVGPRAEYIVEIYGTDGSIRWNFEDLNHLQVCLGRNNRALQGYVNC
MAGPDFPEFMRFQPGAGTSMGFDDMKVVEAAKFVRGVLDGQQYGPSVADGWASAEVNDAIVASCGDHAWHDVKPVSGRTT
FDK

Sequences:

>Translated_403_residues
MGVVMEKRRTIGVGVVSLGWMGRLHTRAYKALAEKFPEIDVNIRLVSCCDVVAENRRQAVDRLGFCTAVEDYHDLIGNPE
VDVVSICAPNFLHRDIALAAAEVGKPFWIEKPMGTNAKQSRQIAEAAEAKGLVTSVGFNYRHTPSIEFARQLIADGRLGT
VTNARAWLIADYASDPHGPLTWRYDRERAGAGVILDLMGHGEDLVQYLLKGRFTEVSAVSETFIRQRPKPLKEGIGHTGW
VVSDELGPVGNEDYCAVIARMENGVMCTLESSRVSVGPRAEYIVEIYGTDGSIRWNFEDLNHLQVCLGRNNRALQGYVNC
MAGPDFPEFMRFQPGAGTSMGFDDMKVVEAAKFVRGVLDGQQYGPSVADGWASAEVNDAIVASCGDHAWHDVKPVSGRTT
FDK
>Mature_402_residues
GVVMEKRRTIGVGVVSLGWMGRLHTRAYKALAEKFPEIDVNIRLVSCCDVVAENRRQAVDRLGFCTAVEDYHDLIGNPEV
DVVSICAPNFLHRDIALAAAEVGKPFWIEKPMGTNAKQSRQIAEAAEAKGLVTSVGFNYRHTPSIEFARQLIADGRLGTV
TNARAWLIADYASDPHGPLTWRYDRERAGAGVILDLMGHGEDLVQYLLKGRFTEVSAVSETFIRQRPKPLKEGIGHTGWV
VSDELGPVGNEDYCAVIARMENGVMCTLESSRVSVGPRAEYIVEIYGTDGSIRWNFEDLNHLQVCLGRNNRALQGYVNCM
AGPDFPEFMRFQPGAGTSMGFDDMKVVEAAKFVRGVLDGQQYGPSVADGWASAEVNDAIVASCGDHAWHDVKPVSGRTTF
DK

Specific function: Unknown

COG id: COG0673

COG function: function code R; Predicted dehydrogenases and related proteins

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the gfo/idh/mocA family [H]

Homologues:

Organism=Escherichia coli, GI1787574, Length=168, Percent_Identity=25.5952380952381, Blast_Score=65, Evalue=8e-12,
Organism=Escherichia coli, GI87082405, Length=293, Percent_Identity=23.2081911262799, Blast_Score=64, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6323975, Length=227, Percent_Identity=26.431718061674, Blast_Score=67, Evalue=6e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016040
- InterPro:   IPR000683
- InterPro:   IPR004104 [H]

Pfam domain/function: PF01408 GFO_IDH_MocA; PF02894 GFO_IDH_MocA_C [H]

EC number: 1.1.1.18

Molecular weight: Translated: 44283; Mature: 44152

Theoretical pI: Translated: 5.83; Mature: 5.83

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGVVMEKRRTIGVGVVSLGWMGRLHTRAYKALAEKFPEIDVNIRLVSCCDVVAENRRQAV
CCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEHHHHHHHHHHHHHH
DRLGFCTAVEDYHDLIGNPEVDVVSICAPNFLHRDIALAAAEVGKPFWIEKPMGTNAKQS
HHHHHHHHHHHHHHHHCCCCCCEEEECCCHHHHHHHHHHHHHCCCCEEEECCCCCCHHHH
RQIAEAAEAKGLVTSVGFNYRHTPSIEFARQLIADGRLGTVTNARAWLIADYASDPHGPL
HHHHHHHHHCCCEEECCCCCCCCCCHHHHHHHHHCCCCCEECCCCEEEEEECCCCCCCCE
TWRYDRERAGAGVILDLMGHGEDLVQYLLKGRFTEVSAVSETFIRQRPKPLKEGIGHTGW
EEEECHHCCCCCEEEEECCCCHHHHHHHHHCCCHHHHHHHHHHHHCCCCHHHHCCCCCCC
VVSDELGPVGNEDYCAVIARMENGVMCTLESSRVSVGPRAEYIVEIYGTDGSIRWNFEDL
EEECCCCCCCCCCCEEEEEECCCCEEEEEECCCCCCCCCCEEEEEEEECCCEEEECHHHC
NHLQVCLGRNNRALQGYVNCMAGPDFPEFMRFQPGAGTSMGFDDMKVVEAAKFVRGVLDG
CCEEEEECCCCCHHHHHHHHCCCCCCHHHHEECCCCCCCCCCCHHHHHHHHHHHHHHHCC
QQYGPSVADGWASAEVNDAIVASCGDHAWHDVKPVSGRTTFDK
HHCCCCHHCCCCCCCCCCHHEECCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
GVVMEKRRTIGVGVVSLGWMGRLHTRAYKALAEKFPEIDVNIRLVSCCDVVAENRRQAV
CCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEHHHHHHHHHHHHHH
DRLGFCTAVEDYHDLIGNPEVDVVSICAPNFLHRDIALAAAEVGKPFWIEKPMGTNAKQS
HHHHHHHHHHHHHHHHCCCCCCEEEECCCHHHHHHHHHHHHHCCCCEEEECCCCCCHHHH
RQIAEAAEAKGLVTSVGFNYRHTPSIEFARQLIADGRLGTVTNARAWLIADYASDPHGPL
HHHHHHHHHCCCEEECCCCCCCCCCHHHHHHHHHCCCCCEECCCCEEEEEECCCCCCCCE
TWRYDRERAGAGVILDLMGHGEDLVQYLLKGRFTEVSAVSETFIRQRPKPLKEGIGHTGW
EEEECHHCCCCCEEEEECCCCHHHHHHHHHCCCHHHHHHHHHHHHCCCCHHHHCCCCCCC
VVSDELGPVGNEDYCAVIARMENGVMCTLESSRVSVGPRAEYIVEIYGTDGSIRWNFEDL
EEECCCCCCCCCCCEEEEEECCCCEEEEEECCCCCCCCCCEEEEEEEECCCEEEECHHHC
NHLQVCLGRNNRALQGYVNCMAGPDFPEFMRFQPGAGTSMGFDDMKVVEAAKFVRGVLDG
CCEEEEECCCCCHHHHHHHHCCCCCCHHHHEECCCCCCCCCCCHHHHHHHHHHHHHHHCC
QQYGPSVADGWASAEVNDAIVASCGDHAWHDVKPVSGRTTFDK
HHCCCCHHCCCCCCCCCCHHEECCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: myo-inositol; NAD+

Specific reaction: myo-inositol + NAD+ = 2,4,6/3,5-pentahydroxycyclohexanone + NADH + H+

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377; 9025289 [H]