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The map label for this gene is iolD1 [H]

Identifier: 50841942

GI number: 50841942

Start: 504802

End: 506700

Strand: Direct

Name: iolD1 [H]

Synonym: PPA0459

Alternate gene names: 50841942

Gene position: 504802-506700 (Clockwise)

Preceding gene: 50841941

Following gene: 50841944

Centisome position: 19.72

GC content: 60.51

Gene sequence:

>1899_bases
ATGACTGAGCAGACTGTGAGGCTCAGCGTCGGACAGGCCATCGTGCGCTATCTGACTCATCAGTACACCGAGCGGGACGG
GGTTGAACGCCGCTTCATACGCGGAGTCTTTGGGATCTTCGGCCATGGAAATGCTACTGGTTTGGGCCAGGCGCTTTTGC
AGAATGAGGCGGCCCCCGACGATGAGGAAGGCCGACTTGAGTACTGGATGGGCCGGAACGAGCAAGGTATGGTTCACGCC
TGTTCCGCCTACGCCCGCGCTACGAACCGGTTGCAGGCGTTAGCTGCCACCGCGTCCATTGGGCCGGGTTCGCTCAACAT
GGTCACCGGTGCGGCTCTCGCCACCACCAATCGGGTACCGGTGTTGCTCTTCCCATCCGACATCTTCGCCAATCGGGCAC
CTGATCCTGTCCTTCAGCAGCTGGAGGACCCCACCAACCCCGATGTATCGTGCAACGACGCGTTCCGTCCGGTCTCGAAG
TTCTGGGATCGAGTGAATCGTCCCGAACAGCTCATGGTTTCGCTGCCTCGTGCGATGCGGGTGCTTACTGATCCTGTGGA
CACGGGAGCTGCCGTCATCTGTCTACCGGAAGACGTACAGACCGAGGTGTACGACTGGCCGGTGTCTTTCTTCGCCAAGA
AGGTGTGGTACATCGATCGCCGCCTCCCCGATAGGGCCGCTGTGGTGCGTGCAGCGCAAGCCATCCGTGCCGCACGCTGT
CCACTCATCATTTCTGGTGGAGGCACGATCTATTCCGAGGCCAGCCAGGAGCTGCGGGACTTCGCATCTGCTACCGGCAT
CCCGGTCGCCGACACCCAGGCGGGGAAAGGTGCAATCAATTGGGATCATCCGTGCGCCGTTGGTGGCGTTGGTGCGACTG
GTTCCGACTCAGCGAATGCGCTGGCGGCCCAGGCCGACCTCATCATCGGCATCGGCACGCGGTACTCCGATTTCACCACC
GGTTCGCAGACGGCCTTTAAAAATCCGGACGTGACGTTCGTCAACGTCAATGTGGGGGCCTTTGATGCCGCCAAGCAGGG
TGCGCAGATGTTGGTGGCCGATGCGCGTGAGGCTCTCACGGCCCTGTCGAAGGAGCTTGACGGCTACCGCGTTCCTCAAG
AATGGAGCGACCTCGCTAGTGCGAAGGCCAAAGCGTGGGCAGAGGTAACCGATCGTTGCTACCACCTGGGGCACGGTCCG
CTCCCGGCTCAGACCGAAGTGTTTGGAGCGCTCAACGAGCTCATGGGTGACGACGACGTGGTCATTAATGCTGCCGGTTC
GATGCCTGGCGACTTGCAGTGTCTGTGGCGTGCCAGCACTCCCGTGCAGTACCACGTGGAGTATGCATTCTCGTGCATGG
GCTACGAGATTCCGGCGTCATTGGGCGTGAAGATGGCGTTGCCGGATTCCGAGGTCGTTGCGATTGTCGGTGATGGCACC
TACCAAATGCTGCCGATGGAGTTGGCAACGATCGTCCAGGAGGGTGCGAAGGTCATTCTCGTATTGCTTCAGAACTACGG
ATTTGCCTCCATCGGGGCGCTGTCGGAGTCTCACGGTTCTCAGCGGTTCGGGACGAAGTACCGCCAGCACGATGACGGTC
AAGGTCATGTGGTCGGGGACGACGCCCTGCTACCCCTTGACATTGCCGCTAACGCGCGGTCGTGGGGTATTGATGTCCTC
GAGGTTCGTACCATCAAGGAGTTCAGGGAGGCTTACCGCAAGGCCGAGGCGTCTGATCATGCCACGATGATCCATATTGA
GACTGACCTGTACGGGCCCAACCCACCTGCATCGGGATGGTGGGATGTACCGGTCACGTCGCAATCGCGCCTGAAGTCGA
CGCAGAAAGCCTATGCCGAGTACACCGGACAGGTTGCCGCACAGCGCAGGTTTTTCTGA

Upstream 100 bases:

>100_bases
CAGTCGACGATCCGCATTACGGGTGGATTCGTCAAACTTGGCAGGACCAAGATATCGACCCGCGCCTGCCGCTAGCGGTG
GACGACTCAAAGGAGTGATT

Downstream 100 bases:

>100_bases
CCGCATCCCCGATTCCCGGTTGGGGATTAGGCGACGTGTCGTAGTGACGGTACGAGGAGATCCAGTTTTGGCTGGATCCC
CTCGTGAGTCCGGTTATCTT

Product: thiamine pyrophosphate-requiring enzyme, putative acetolactate synthase

Products: pyruvate; 2-aceto-2-hydroxy-butyrate; CO2 [C]

Alternate protein names: THcHDO hydrolase 1 [H]

Number of amino acids: Translated: 632; Mature: 631

Protein sequence:

>632_residues
MTEQTVRLSVGQAIVRYLTHQYTERDGVERRFIRGVFGIFGHGNATGLGQALLQNEAAPDDEEGRLEYWMGRNEQGMVHA
CSAYARATNRLQALAATASIGPGSLNMVTGAALATTNRVPVLLFPSDIFANRAPDPVLQQLEDPTNPDVSCNDAFRPVSK
FWDRVNRPEQLMVSLPRAMRVLTDPVDTGAAVICLPEDVQTEVYDWPVSFFAKKVWYIDRRLPDRAAVVRAAQAIRAARC
PLIISGGGTIYSEASQELRDFASATGIPVADTQAGKGAINWDHPCAVGGVGATGSDSANALAAQADLIIGIGTRYSDFTT
GSQTAFKNPDVTFVNVNVGAFDAAKQGAQMLVADAREALTALSKELDGYRVPQEWSDLASAKAKAWAEVTDRCYHLGHGP
LPAQTEVFGALNELMGDDDVVINAAGSMPGDLQCLWRASTPVQYHVEYAFSCMGYEIPASLGVKMALPDSEVVAIVGDGT
YQMLPMELATIVQEGAKVILVLLQNYGFASIGALSESHGSQRFGTKYRQHDDGQGHVVGDDALLPLDIAANARSWGIDVL
EVRTIKEFREAYRKAEASDHATMIHIETDLYGPNPPASGWWDVPVTSQSRLKSTQKAYAEYTGQVAAQRRFF

Sequences:

>Translated_632_residues
MTEQTVRLSVGQAIVRYLTHQYTERDGVERRFIRGVFGIFGHGNATGLGQALLQNEAAPDDEEGRLEYWMGRNEQGMVHA
CSAYARATNRLQALAATASIGPGSLNMVTGAALATTNRVPVLLFPSDIFANRAPDPVLQQLEDPTNPDVSCNDAFRPVSK
FWDRVNRPEQLMVSLPRAMRVLTDPVDTGAAVICLPEDVQTEVYDWPVSFFAKKVWYIDRRLPDRAAVVRAAQAIRAARC
PLIISGGGTIYSEASQELRDFASATGIPVADTQAGKGAINWDHPCAVGGVGATGSDSANALAAQADLIIGIGTRYSDFTT
GSQTAFKNPDVTFVNVNVGAFDAAKQGAQMLVADAREALTALSKELDGYRVPQEWSDLASAKAKAWAEVTDRCYHLGHGP
LPAQTEVFGALNELMGDDDVVINAAGSMPGDLQCLWRASTPVQYHVEYAFSCMGYEIPASLGVKMALPDSEVVAIVGDGT
YQMLPMELATIVQEGAKVILVLLQNYGFASIGALSESHGSQRFGTKYRQHDDGQGHVVGDDALLPLDIAANARSWGIDVL
EVRTIKEFREAYRKAEASDHATMIHIETDLYGPNPPASGWWDVPVTSQSRLKSTQKAYAEYTGQVAAQRRFF
>Mature_631_residues
TEQTVRLSVGQAIVRYLTHQYTERDGVERRFIRGVFGIFGHGNATGLGQALLQNEAAPDDEEGRLEYWMGRNEQGMVHAC
SAYARATNRLQALAATASIGPGSLNMVTGAALATTNRVPVLLFPSDIFANRAPDPVLQQLEDPTNPDVSCNDAFRPVSKF
WDRVNRPEQLMVSLPRAMRVLTDPVDTGAAVICLPEDVQTEVYDWPVSFFAKKVWYIDRRLPDRAAVVRAAQAIRAARCP
LIISGGGTIYSEASQELRDFASATGIPVADTQAGKGAINWDHPCAVGGVGATGSDSANALAAQADLIIGIGTRYSDFTTG
SQTAFKNPDVTFVNVNVGAFDAAKQGAQMLVADAREALTALSKELDGYRVPQEWSDLASAKAKAWAEVTDRCYHLGHGPL
PAQTEVFGALNELMGDDDVVINAAGSMPGDLQCLWRASTPVQYHVEYAFSCMGYEIPASLGVKMALPDSEVVAIVGDGTY
QMLPMELATIVQEGAKVILVLLQNYGFASIGALSESHGSQRFGTKYRQHDDGQGHVVGDDALLPLDIAANARSWGIDVLE
VRTIKEFREAYRKAEASDHATMIHIETDLYGPNPPASGWWDVPVTSQSRLKSTQKAYAEYTGQVAAQRRFF

Specific function: Involved in the cleavage of the C1-C2 bond of 3D- (3,5/4)-trihydroxycyclohexane-1,2-dione (THcHDO) to yield 5-deoxy- glucuronate (5DG) [H]

COG id: COG3962

COG function: function code E; Acetolactate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Homo sapiens, GI93004078, Length=463, Percent_Identity=24.4060475161987, Blast_Score=88, Evalue=3e-17,
Organism=Escherichia coli, GI87081685, Length=473, Percent_Identity=26.0042283298097, Blast_Score=136, Evalue=5e-33,
Organism=Escherichia coli, GI1786717, Length=476, Percent_Identity=26.890756302521, Blast_Score=113, Evalue=4e-26,
Organism=Escherichia coli, GI1790104, Length=487, Percent_Identity=24.6406570841889, Blast_Score=108, Evalue=8e-25,
Organism=Escherichia coli, GI1787096, Length=511, Percent_Identity=23.4833659491194, Blast_Score=96, Evalue=9e-21,
Organism=Escherichia coli, GI1788716, Length=423, Percent_Identity=24.113475177305, Blast_Score=82, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI17531299, Length=551, Percent_Identity=24.6823956442831, Blast_Score=78, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI17531301, Length=551, Percent_Identity=24.6823956442831, Blast_Score=77, Evalue=2e-14,
Organism=Saccharomyces cerevisiae, GI6323755, Length=553, Percent_Identity=22.7848101265823, Blast_Score=112, Evalue=2e-25,
Organism=Saccharomyces cerevisiae, GI6323163, Length=294, Percent_Identity=28.5714285714286, Blast_Score=81, Evalue=6e-16,
Organism=Saccharomyces cerevisiae, GI6321524, Length=307, Percent_Identity=25.0814332247557, Blast_Score=70, Evalue=1e-12,
Organism=Drosophila melanogaster, GI19922626, Length=462, Percent_Identity=23.3766233766234, Blast_Score=83, Evalue=7e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR000399
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: 2.2.1.6 [C]

Molecular weight: Translated: 68423; Mature: 68291

Theoretical pI: Translated: 4.90; Mature: 4.90

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTEQTVRLSVGQAIVRYLTHQYTERDGVERRFIRGVFGIFGHGNATGLGQALLQNEAAPD
CCCCEEEHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCC
DEEGRLEYWMGRNEQGMVHACSAYARATNRLQALAATASIGPGSLNMVTGAALATTNRVP
CCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHCCHHHEECCCCE
VLLFPSDIFANRAPDPVLQQLEDPTNPDVSCNDAFRPVSKFWDRVNRPEQLMVSLPRAMR
EEEECCHHHCCCCCCHHHHHHCCCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHH
VLTDPVDTGAAVICLPEDVQTEVYDWPVSFFAKKVWYIDRRLPDRAAVVRAAQAIRAARC
HHCCCCCCCCEEEEECCCCCCHHHHCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCC
PLIISGGGTIYSEASQELRDFASATGIPVADTQAGKGAINWDHPCAVGGVGATGSDSANA
CEEEECCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCH
LAAQADLIIGIGTRYSDFTTGSQTAFKNPDVTFVNVNVGAFDAAKQGAQMLVADAREALT
HHEECCEEEEECCCCCCCCCCCCCCCCCCCEEEEEEECCCCHHHHHCHHHHHHHHHHHHH
ALSKELDGYRVPQEWSDLASAKAKAWAEVTDRCYHLGHGPLPAQTEVFGALNELMGDDDV
HHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCE
VINAAGSMPGDLQCLWRASTPVQYHVEYAFSCMGYEIPASLGVKMALPDSEVVAIVGDGT
EEEECCCCCCCEEEEEECCCCEEEEEEEHHHHCCEECCHHCCEEEECCCCCEEEEEECCC
YQMLPMELATIVQEGAKVILVLLQNYGFASIGALSESHGSQRFGTKYRQHDDGQGHVVGD
EEEHHHHHHHHHHCCHHEEHHHHHCCCCHHHHCCHHCCCCHHHCCHHHCCCCCCCEEECC
DALLPLDIAANARSWGIDVLEVRTIKEFREAYRKAEASDHATMIHIETDLYGPNPPASGW
CCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCEEEEEEEECCCCCCCCCCCE
WDVPVTSQSRLKSTQKAYAEYTGQVAAQRRFF
ECCCCCCHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
TEQTVRLSVGQAIVRYLTHQYTERDGVERRFIRGVFGIFGHGNATGLGQALLQNEAAPD
CCCEEEHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCC
DEEGRLEYWMGRNEQGMVHACSAYARATNRLQALAATASIGPGSLNMVTGAALATTNRVP
CCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHCCHHHEECCCCE
VLLFPSDIFANRAPDPVLQQLEDPTNPDVSCNDAFRPVSKFWDRVNRPEQLMVSLPRAMR
EEEECCHHHCCCCCCHHHHHHCCCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHH
VLTDPVDTGAAVICLPEDVQTEVYDWPVSFFAKKVWYIDRRLPDRAAVVRAAQAIRAARC
HHCCCCCCCCEEEEECCCCCCHHHHCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCC
PLIISGGGTIYSEASQELRDFASATGIPVADTQAGKGAINWDHPCAVGGVGATGSDSANA
CEEEECCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCH
LAAQADLIIGIGTRYSDFTTGSQTAFKNPDVTFVNVNVGAFDAAKQGAQMLVADAREALT
HHEECCEEEEECCCCCCCCCCCCCCCCCCCEEEEEEECCCCHHHHHCHHHHHHHHHHHHH
ALSKELDGYRVPQEWSDLASAKAKAWAEVTDRCYHLGHGPLPAQTEVFGALNELMGDDDV
HHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCE
VINAAGSMPGDLQCLWRASTPVQYHVEYAFSCMGYEIPASLGVKMALPDSEVVAIVGDGT
EEEECCCCCCCEEEEEECCCCEEEEEEEHHHHCCEECCHHCCEEEECCCCCEEEEEECCC
YQMLPMELATIVQEGAKVILVLLQNYGFASIGALSESHGSQRFGTKYRQHDDGQGHVVGD
EEEHHHHHHHHHHCCHHEEHHHHHCCCCHHHHCCHHCCCCHHHCCHHHCCCCCCCEEECC
DALLPLDIAANARSWGIDVLEVRTIKEFREAYRKAEASDHATMIHIETDLYGPNPPASGW
CCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCEEEEEEEECCCCCCCCCCCE
WDVPVTSQSRLKSTQKAYAEYTGQVAAQRRFF
ECCCCCCHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: FAD [C]

Metal ions: Mg2+ [C]

Kcat value (1/min): 4002 [C]

Specific activity: 53

Km value (mM): 7.6 {pyruvate}} 7 {pyruvate}} 2.9 {pyruvate}} 2.6 {pyruvate}} 1.3 {pyruvate}} 1 {pyruvate}} 5 {2-oxobutanoate}} 1 {2-oxobutanoate}} [C]

Substrates: acetolactate; CO2; pyruvate; 2-oxobutanoate [C]

Specific reaction: acetolactate + CO2 = 2 pyruvate pyruvate + 2-oxobutanoate = 2-aceto-2-hydroxy-butyrate + CO2 [C]

General reaction: C-C bond formation; decarboxylation [C]

Inhibitor: @OXOB02.txt*2-oxobutanoate!; chlorimuronethyl; chlorsulphuron; Ile; imazapyr; Leu; Val [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA