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The map label for this gene is ansP [H]

Identifier: 50841853

GI number: 50841853

Start: 414841

End: 416289

Strand: Reverse

Name: ansP [H]

Synonym: PPA0368

Alternate gene names: 50841853

Gene position: 416289-414841 (Counterclockwise)

Preceding gene: 50841854

Following gene: 50841852

Centisome position: 16.26

GC content: 60.11

Gene sequence:

>1449_bases
ATGCAGGAAGGTGATTCTGTCACCCCGCACACCAGCACCACCGTTGATAAGGGGGATGCCGGCTACGACAAGGCCTTAAA
GAACAGGCACCTGCAGATGATCGCCATCGGCGGCTCAATCGGCACCGGCTTGTTTCTGGGTACTGGCGGACGCCTTGCCC
AAGGTGGCCCCGGATTGGCGATAAGCTACGCCGTCTGTGGCATCTTCGCCTTCCTCATGGTGAGGGCCCTCGGTGAGATG
GCTATTCGTCGTCCGTCCTCAGGCGCCTTCGTGTCCTACGCGCGTGAATTCATGGGTGAGAAGGGCGCCTACGCCACCGG
ATGGTTCTTCTTCCTTGACTGGTCGGTCACGGTGATGGCTGACATCACCGCAGTGGCGCTGTACCTGCATTATTGGACGC
CCTTGCGTGCCATCCCACAATGGGTGTTAGCGCTCATCGCCCTCGGCGTGGTGTTTACCCTCAACATGTTGAGCGTGAAG
ATGTTCGGCGAGGCCGAATTTTGGTTCGCTGCTATCAAGGTAGCCGCGATCATCCTGTTCATGATCGCGGCCATCTGGGC
CATCGTCACGGGTGCGCGGGTTGGCAACTACACCGCAGGCCTTCACAACATCGCCCAGCATGGCGGATTCTTCCCCGCTG
GGCTGGCGCCGGTCTTTGCACTCACTCTGGGAGTGATCTTCGCCTTCGGCGGCACCGAGATGGTCGGCGTAGCAGCAGGC
GAGACCAAGGATGCGAGCAAGATCTTGCCCAAGGCCATCAACTCGATGATCCTGCGTATCTTCGTCTTCTACGTCGGTTC
GATCATCTTGATGGCGCTGGTACTGCCGTACACTGCCTATTCAAGCAATGAATCCCCGTTCGTCACCTTCTTCACCGGAA
TCGGAATTCCCTACGCTGGTGACATCATCCAGGTAATCGTCCTGACGGCTGCCCTCAGCTCGCTCAATGCTGGCCTGTAC
TCGACCGGACGCACCCTGAGGTCATTGGCGGTTGCCGGCGAAGCACCAAAAGCCGCTGCTAAACTCAACCGTCACCACGT
GCCTTCTGGCGGCATCGCCATGACCGCCGCCCTCGGTCTGCTGGGCGTTGCCCTCAACGCCTTCCTCCCGGATAGCGCCT
TCGAGATCGTCATGAACCTCGCCGGCATCGGTATCGCCGGCACGTGGGCGATGGTCCTGCTCGCCCACACCCGTTTCGTG
AGCGCAGTGAGGCGGGGCAAGGACAACCGACCGGAATACCGCATGCCTGGTGCCCCGGTCACGAACTACATCTCATTGGC
CTTCCTGCTGATCGTCGTACTGTCGAACGTCACTTCGGAATCGGGGCGCTGGACACTAGCCCTCTTCGCCCTGGTCGCAA
TTGCCATGGTCGCCGGGTGGTACGCAGTCCGTAACAGCATCAAACCCGACCTGCTCGACAACGTGGTTCCAAGCGATGAG
GGGATGTGA

Upstream 100 bases:

>100_bases
CTGATCCGTTTGTAAAGATACTTATTTTCGGTGCAGAGTGGCCCCTTCGGGGCTAGCATTCCCGTGCTAGACCGGGTGGT
TCCGTCACGAGGAGGTTCCC

Downstream 100 bases:

>100_bases
ATGATGCAAACCCACGTTCTCTACACCGGCGGAACGTTAGGAATGGTGTCCACTCCTAACGGCTTAGCTCCAGGTGCTGA
TCTGACGGGTTGGCTTAACC

Product: amino acid permease

Products: Proton [Cytoplasm]; L-asparagine [Cytoplasm] [C]

Alternate protein names: L-asparagine transport protein [H]

Number of amino acids: Translated: 482; Mature: 482

Protein sequence:

>482_residues
MQEGDSVTPHTSTTVDKGDAGYDKALKNRHLQMIAIGGSIGTGLFLGTGGRLAQGGPGLAISYAVCGIFAFLMVRALGEM
AIRRPSSGAFVSYAREFMGEKGAYATGWFFFLDWSVTVMADITAVALYLHYWTPLRAIPQWVLALIALGVVFTLNMLSVK
MFGEAEFWFAAIKVAAIILFMIAAIWAIVTGARVGNYTAGLHNIAQHGGFFPAGLAPVFALTLGVIFAFGGTEMVGVAAG
ETKDASKILPKAINSMILRIFVFYVGSIILMALVLPYTAYSSNESPFVTFFTGIGIPYAGDIIQVIVLTAALSSLNAGLY
STGRTLRSLAVAGEAPKAAAKLNRHHVPSGGIAMTAALGLLGVALNAFLPDSAFEIVMNLAGIGIAGTWAMVLLAHTRFV
SAVRRGKDNRPEYRMPGAPVTNYISLAFLLIVVLSNVTSESGRWTLALFALVAIAMVAGWYAVRNSIKPDLLDNVVPSDE
GM

Sequences:

>Translated_482_residues
MQEGDSVTPHTSTTVDKGDAGYDKALKNRHLQMIAIGGSIGTGLFLGTGGRLAQGGPGLAISYAVCGIFAFLMVRALGEM
AIRRPSSGAFVSYAREFMGEKGAYATGWFFFLDWSVTVMADITAVALYLHYWTPLRAIPQWVLALIALGVVFTLNMLSVK
MFGEAEFWFAAIKVAAIILFMIAAIWAIVTGARVGNYTAGLHNIAQHGGFFPAGLAPVFALTLGVIFAFGGTEMVGVAAG
ETKDASKILPKAINSMILRIFVFYVGSIILMALVLPYTAYSSNESPFVTFFTGIGIPYAGDIIQVIVLTAALSSLNAGLY
STGRTLRSLAVAGEAPKAAAKLNRHHVPSGGIAMTAALGLLGVALNAFLPDSAFEIVMNLAGIGIAGTWAMVLLAHTRFV
SAVRRGKDNRPEYRMPGAPVTNYISLAFLLIVVLSNVTSESGRWTLALFALVAIAMVAGWYAVRNSIKPDLLDNVVPSDE
GM
>Mature_482_residues
MQEGDSVTPHTSTTVDKGDAGYDKALKNRHLQMIAIGGSIGTGLFLGTGGRLAQGGPGLAISYAVCGIFAFLMVRALGEM
AIRRPSSGAFVSYAREFMGEKGAYATGWFFFLDWSVTVMADITAVALYLHYWTPLRAIPQWVLALIALGVVFTLNMLSVK
MFGEAEFWFAAIKVAAIILFMIAAIWAIVTGARVGNYTAGLHNIAQHGGFFPAGLAPVFALTLGVIFAFGGTEMVGVAAG
ETKDASKILPKAINSMILRIFVFYVGSIILMALVLPYTAYSSNESPFVTFFTGIGIPYAGDIIQVIVLTAALSSLNAGLY
STGRTLRSLAVAGEAPKAAAKLNRHHVPSGGIAMTAALGLLGVALNAFLPDSAFEIVMNLAGIGIAGTWAMVLLAHTRFV
SAVRRGKDNRPEYRMPGAPVTNYISLAFLLIVVLSNVTSESGRWTLALFALVAIAMVAGWYAVRNSIKPDLLDNVVPSDE
GM

Specific function: Unknown

COG id: COG1113

COG function: function code E; Gamma-aminobutyrate permease and related permeases

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amino acid-polyamine-organocation (APC) superfamily. Amino acid transporter (AAT) (TC 2.A.3.1) family [H]

Homologues:

Organism=Homo sapiens, GI4507047, Length=424, Percent_Identity=24.0566037735849, Blast_Score=67, Evalue=5e-11,
Organism=Escherichia coli, GI87081915, Length=483, Percent_Identity=48.6542443064182, Blast_Score=468, Evalue=1e-133,
Organism=Escherichia coli, GI1786789, Length=444, Percent_Identity=35.8108108108108, Blast_Score=298, Evalue=6e-82,
Organism=Escherichia coli, GI1786302, Length=410, Percent_Identity=38.2926829268293, Blast_Score=295, Evalue=4e-81,
Organism=Escherichia coli, GI1790653, Length=451, Percent_Identity=36.3636363636364, Blast_Score=281, Evalue=6e-77,
Organism=Escherichia coli, GI48994972, Length=426, Percent_Identity=36.3849765258216, Blast_Score=276, Evalue=2e-75,
Organism=Escherichia coli, GI1786602, Length=413, Percent_Identity=36.8038740920097, Blast_Score=266, Evalue=2e-72,
Organism=Escherichia coli, GI87081708, Length=422, Percent_Identity=33.4123222748815, Blast_Score=260, Evalue=1e-70,
Organism=Escherichia coli, GI1788480, Length=436, Percent_Identity=35.7798165137615, Blast_Score=258, Evalue=6e-70,
Organism=Escherichia coli, GI1789017, Length=410, Percent_Identity=30.2439024390244, Blast_Score=205, Evalue=4e-54,
Organism=Escherichia coli, GI87081869, Length=261, Percent_Identity=22.9885057471264, Blast_Score=65, Evalue=1e-11,
Organism=Saccharomyces cerevisiae, GI6324061, Length=455, Percent_Identity=29.6703296703297, Blast_Score=210, Evalue=5e-55,
Organism=Saccharomyces cerevisiae, GI6320772, Length=446, Percent_Identity=30.7174887892377, Blast_Score=202, Evalue=1e-52,
Organism=Saccharomyces cerevisiae, GI6322892, Length=426, Percent_Identity=32.6291079812207, Blast_Score=188, Evalue=1e-48,
Organism=Saccharomyces cerevisiae, GI6324553, Length=403, Percent_Identity=31.2655086848635, Blast_Score=174, Evalue=2e-44,
Organism=Saccharomyces cerevisiae, GI6324990, Length=447, Percent_Identity=30.8724832214765, Blast_Score=172, Evalue=1e-43,
Organism=Saccharomyces cerevisiae, GI6324924, Length=461, Percent_Identity=30.1518438177874, Blast_Score=170, Evalue=5e-43,
Organism=Saccharomyces cerevisiae, GI6324059, Length=458, Percent_Identity=29.9126637554585, Blast_Score=169, Evalue=8e-43,
Organism=Saccharomyces cerevisiae, GI6322967, Length=430, Percent_Identity=28.6046511627907, Blast_Score=164, Evalue=3e-41,
Organism=Saccharomyces cerevisiae, GI6320717, Length=411, Percent_Identity=27.007299270073, Blast_Score=160, Evalue=3e-40,
Organism=Saccharomyces cerevisiae, GI6321053, Length=422, Percent_Identity=28.436018957346, Blast_Score=160, Evalue=5e-40,
Organism=Saccharomyces cerevisiae, GI6319824, Length=417, Percent_Identity=26.8585131894484, Blast_Score=156, Evalue=5e-39,
Organism=Saccharomyces cerevisiae, GI6320251, Length=419, Percent_Identity=25.7756563245823, Blast_Score=150, Evalue=5e-37,
Organism=Saccharomyces cerevisiae, GI6321629, Length=446, Percent_Identity=27.5784753363229, Blast_Score=150, Evalue=6e-37,
Organism=Saccharomyces cerevisiae, GI6324981, Length=419, Percent_Identity=28.1622911694511, Blast_Score=145, Evalue=2e-35,
Organism=Saccharomyces cerevisiae, GI6319608, Length=433, Percent_Identity=28.175519630485, Blast_Score=143, Evalue=5e-35,
Organism=Saccharomyces cerevisiae, GI6319542, Length=399, Percent_Identity=26.8170426065163, Blast_Score=141, Evalue=2e-34,
Organism=Saccharomyces cerevisiae, GI6319543, Length=396, Percent_Identity=26.2626262626263, Blast_Score=136, Evalue=6e-33,
Organism=Saccharomyces cerevisiae, GI6320364, Length=490, Percent_Identity=22.8571428571429, Blast_Score=77, Evalue=4e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004841
- InterPro:   IPR002293
- InterPro:   IPR004840 [H]

Pfam domain/function: PF00324 AA_permease [H]

EC number: NA

Molecular weight: Translated: 51081; Mature: 51081

Theoretical pI: Translated: 9.44; Mature: 9.44

Prosite motif: PS00218 AMINO_ACID_PERMEASE_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQEGDSVTPHTSTTVDKGDAGYDKALKNRHLQMIAIGGSIGTGLFLGTGGRLAQGGPGLA
CCCCCCCCCCCCCCCCCCCCCHHHHHHCCCEEEEEECCCCCCCEEEECCCCCCCCCCCHH
ISYAVCGIFAFLMVRALGEMAIRRPSSGAFVSYAREFMGEKGAYATGWFFFLDWSVTVMA
HHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCEEEEEEEEEECHHHHHH
DITAVALYLHYWTPLRAIPQWVLALIALGVVFTLNMLSVKMFGEAEFWFAAIKVAAIILF
HHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHH
MIAAIWAIVTGARVGNYTAGLHNIAQHGGFFPAGLAPVFALTLGVIFAFGGTEMVGVAAG
HHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEECC
ETKDASKILPKAINSMILRIFVFYVGSIILMALVLPYTAYSSNESPFVTFFTGIGIPYAG
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCHH
DIIQVIVLTAALSSLNAGLYSTGRTLRSLAVAGEAPKAAAKLNRHHVPSGGIAMTAALGL
HHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCHHHHHHHHH
LGVALNAFLPDSAFEIVMNLAGIGIAGTWAMVLLAHTRFVSAVRRGKDNRPEYRMPGAPV
HHHHHHHHCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCH
TNYISLAFLLIVVLSNVTSESGRWTLALFALVAIAMVAGWYAVRNSIKPDLLDNVVPSDE
HHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCCCCC
GM
CC
>Mature Secondary Structure
MQEGDSVTPHTSTTVDKGDAGYDKALKNRHLQMIAIGGSIGTGLFLGTGGRLAQGGPGLA
CCCCCCCCCCCCCCCCCCCCCHHHHHHCCCEEEEEECCCCCCCEEEECCCCCCCCCCCHH
ISYAVCGIFAFLMVRALGEMAIRRPSSGAFVSYAREFMGEKGAYATGWFFFLDWSVTVMA
HHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCEEEEEEEEEECHHHHHH
DITAVALYLHYWTPLRAIPQWVLALIALGVVFTLNMLSVKMFGEAEFWFAAIKVAAIILF
HHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHH
MIAAIWAIVTGARVGNYTAGLHNIAQHGGFFPAGLAPVFALTLGVIFAFGGTEMVGVAAG
HHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEECC
ETKDASKILPKAINSMILRIFVFYVGSIILMALVLPYTAYSSNESPFVTFFTGIGIPYAG
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCHH
DIIQVIVLTAALSSLNAGLYSTGRTLRSLAVAGEAPKAAAKLNRHHVPSGGIAMTAALGL
HHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCHHHHHHHHH
LGVALNAFLPDSAFEIVMNLAGIGIAGTWAMVLLAHTRFVSAVRRGKDNRPEYRMPGAPV
HHHHHHHHCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCH
TNYISLAFLLIVVLSNVTSESGRWTLALFALVAIAMVAGWYAVRNSIKPDLLDNVVPSDE
HHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCCCCC
GM
CC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: L-asparagine [Periplasm]; Proton [Periplasm] [C]

Specific reaction: L-asparagine [Periplasm] + Proton [Periplasm] = Proton [Cytoplasm] + L-asparagine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 12000953 [H]