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The map label for this gene is liaR [H]

Identifier: 50841778

GI number: 50841778

Start: 338575

End: 339231

Strand: Reverse

Name: liaR [H]

Synonym: PPA0289

Alternate gene names: 50841778

Gene position: 339231-338575 (Counterclockwise)

Preceding gene: 50841779

Following gene: 50841777

Centisome position: 13.25

GC content: 63.17

Gene sequence:

>657_bases
ATGCTGCCCGTTCGCGTCTTACTCGTCGACGACGACACGCTCGTACGCAGGGCTGTGCGCCAGATCCTTGAGGCCGCTGA
TGACCTTGAGGTGGTCGGTGAGGCCAGTGACGGCGACGGAGCCGTGAGGCAGGCTCCCCAGGCCGATGTGGTCCTCATGG
ACCTGCGAATGCCCGGAATGAGTGGTGTGGAAGCAACATCGCGAATCACCCATTCGCCGAGTTCACCCAAGGTCGTCGTC
CTCACTACCGTCACCGCCGACGCGGCCATCACCGACGCGGTTCACGCCGGGGCATCGGGGTTCCTACTGAAAGCCTCCTC
ACCTGATGACATCGTCAATGCCGTGCGTGCGGTCCACGCTGGGGATGCCTTCATGTCCCCGACGTCGACCCGCCAACTTC
TGGAACAGTTGCGGGGCGACGCTGGTAGACGGGTCCGCGGGGCTGCCCGCAAGACCCTTTCCGCCCTCACTGACCGGGAG
CGAGAGGTTGCAATAGCGGTGGCCGAGGGACTAACCAATGACGCCATCGCCGCGCGGTTAAACGTTTCGGCAAGCACCGT
CAAGGCGCATCTGTCGACGATTCAGACCAAGCTCGACGTCGACAGTCGAGTCCGCATCGCGGTACTCGTTGAGCGGGCTG
GGTATCTTGCTGCATAA

Upstream 100 bases:

>100_bases
GTGGACATCCCGTGGCAGTGATAATCATCCCCGCCCGTTTCCAATAGACCACAGTGGTGCCGCGACGGATCGCAAACGAT
AGACATCATAGGATGTGGCC

Downstream 100 bases:

>100_bases
GCGCAACGGCGCTGATAGCTTGGGGCTACTGCGTGGAACTACTTACCTGATGACGGGCTGACCGCCTCCATCGTTGCTTT
CCCTGGCCCAAGGCGACGAG

Product: putative two component response regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 218; Mature: 218

Protein sequence:

>218_residues
MLPVRVLLVDDDTLVRRAVRQILEAADDLEVVGEASDGDGAVRQAPQADVVLMDLRMPGMSGVEATSRITHSPSSPKVVV
LTTVTADAAITDAVHAGASGFLLKASSPDDIVNAVRAVHAGDAFMSPTSTRQLLEQLRGDAGRRVRGAARKTLSALTDRE
REVAIAVAEGLTNDAIAARLNVSASTVKAHLSTIQTKLDVDSRVRIAVLVERAGYLAA

Sequences:

>Translated_218_residues
MLPVRVLLVDDDTLVRRAVRQILEAADDLEVVGEASDGDGAVRQAPQADVVLMDLRMPGMSGVEATSRITHSPSSPKVVV
LTTVTADAAITDAVHAGASGFLLKASSPDDIVNAVRAVHAGDAFMSPTSTRQLLEQLRGDAGRRVRGAARKTLSALTDRE
REVAIAVAEGLTNDAIAARLNVSASTVKAHLSTIQTKLDVDSRVRIAVLVERAGYLAA
>Mature_218_residues
MLPVRVLLVDDDTLVRRAVRQILEAADDLEVVGEASDGDGAVRQAPQADVVLMDLRMPGMSGVEATSRITHSPSSPKVVV
LTTVTADAAITDAVHAGASGFLLKASSPDDIVNAVRAVHAGDAFMSPTSTRQLLEQLRGDAGRRVRGAARKTLSALTDRE
REVAIAVAEGLTNDAIAARLNVSASTVKAHLSTIQTKLDVDSRVRIAVLVERAGYLAA

Specific function: Member of the two-component regulatory system liaS/liaR probably involved in response to a subset of cell wall-active antibiotics that interfere with the lipid II cycle in the cytoplasmic membrane (bacitracin, nisin, ramoplanin and vancomycin). Seems also

COG id: COG2197

COG function: function code TK; Response regulator containing a CheY-like receiver domain and an HTH DNA-binding domain

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1788521, Length=210, Percent_Identity=38.0952380952381, Blast_Score=129, Evalue=1e-31,
Organism=Escherichia coli, GI1788222, Length=218, Percent_Identity=33.4862385321101, Blast_Score=127, Evalue=5e-31,
Organism=Escherichia coli, GI1787473, Length=212, Percent_Identity=34.4339622641509, Blast_Score=114, Evalue=4e-27,
Organism=Escherichia coli, GI1790102, Length=206, Percent_Identity=33.0097087378641, Blast_Score=99, Evalue=3e-22,
Organism=Escherichia coli, GI1786747, Length=209, Percent_Identity=28.2296650717703, Blast_Score=90, Evalue=1e-19,
Organism=Escherichia coli, GI1788546, Length=206, Percent_Identity=30.0970873786408, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI1788712, Length=208, Percent_Identity=23.5576923076923, Blast_Score=75, Evalue=2e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR016032
- InterPro:   IPR001789
- InterPro:   IPR000792
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00196 GerE; PF00072 Response_reg [H]

EC number: NA

Molecular weight: Translated: 22894; Mature: 22894

Theoretical pI: Translated: 6.13; Mature: 6.13

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS50043 HTH_LUXR_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLPVRVLLVDDDTLVRRAVRQILEAADDLEVVGEASDGDGAVRQAPQADVVLMDLRMPGM
CCCEEEEEECCHHHHHHHHHHHHHHCCCCEEEECCCCCCCHHHCCCCCCEEEEEEECCCC
SGVEATSRITHSPSSPKVVVLTTVTADAAITDAVHAGASGFLLKASSPDDIVNAVRAVHA
CCCCHHHHHCCCCCCCEEEEEEEECCCHHHHHHHHCCCCCEEEECCCCHHHHHHHHHHHC
GDAFMSPTSTRQLLEQLRGDAGRRVRGAARKTLSALTDREREVAIAVAEGLTNDAIAARL
CCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCHHEEEEEECCCCCCHHEEEE
NVSASTVKAHLSTIQTKLDVDSRVRIAVLVERAGYLAA
CCCHHHHHHHHHHHHHHCCCCCCEEEEEEEECCCCCCC
>Mature Secondary Structure
MLPVRVLLVDDDTLVRRAVRQILEAADDLEVVGEASDGDGAVRQAPQADVVLMDLRMPGM
CCCEEEEEECCHHHHHHHHHHHHHHCCCCEEEECCCCCCCHHHCCCCCCEEEEEEECCCC
SGVEATSRITHSPSSPKVVVLTTVTADAAITDAVHAGASGFLLKASSPDDIVNAVRAVHA
CCCCHHHHHCCCCCCCEEEEEEEECCCHHHHHHHHCCCCCEEEECCCCHHHHHHHHHHHC
GDAFMSPTSTRQLLEQLRGDAGRRVRGAARKTLSALTDREREVAIAVAEGLTNDAIAARL
CCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCHHEEEEEECCCCCCHHEEEE
NVSASTVKAHLSTIQTKLDVDSRVRIAVLVERAGYLAA
CCCHHHHHHHHHHHHHHCCCCCCEEEEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9639930; 9384377 [H]