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The map label for this gene is leuS [H]

Identifier: 50365307

GI number: 50365307

Start: 574124

End: 576529

Strand: Reverse

Name: leuS [H]

Synonym: Mfl490

Alternate gene names: 50365307

Gene position: 576529-574124 (Counterclockwise)

Preceding gene: 50365308

Following gene: 50365306

Centisome position: 72.68

GC content: 29.18

Gene sequence:

>2406_bases
ATGGAATTTTCACATAAAGCGATTGAAAAGAAATGAAAAAAATATTGAGAAGAAAATAATACAAATAAAACAACTAATAC
ATCTGATAAAAAATCTTATGTATTGGATATGTTCCCATACCCAAGTGGAGCAGGTATTCATGTTGGTCATGTTAAAGGTT
ATACTGCAACTGATGTTTTTTCTCGTTATAAAAGAATGAATGGATATGATGTTTTACACCCAATGGGTTGAGATGCATTT
GGATTACCAGCAGAACAATATGCATTAAAAACAGGTAATGATCCAATTGATTTCACTTTAGAAAATATTAAAACATTTAA
ACGCCAATTAAAAATGATGGGATTTAGTTATGATTTTGATAAAGAAATATCAACAGCTAATCCAAACTATTACAAAATAA
CACAATGAATATTTAATCAACTGTATAAAAAAGGTTTAGCAGAAAACCGTGATGTTGAAGTTAACTGATGTCAAGAATTA
GGAACTGTTTTAGCTAATGATGAAATTATTGAAAAAGATGGATTAATGGTTAGTGAACGTGGAGAGCATCCTGTAACTAA
ACGTAAAATGCGTCAATGAGTTTTAAAAATTACAGAATATGCTGATCGTTTATTAGAAGGATTAGATGAATTAGAATGAA
ATAGTTCAATCAAAGACTTACAAAGAAACTGAATTGGTAAATCAACTGGTGTTGAATTAGACTTTTTAGTTAACAATATT
AAAGTTCCTGTATTCACTACTAGAATAGACACTATCTATGGAGTTAGTTATATTGTTTTAGCTCCAGAGCATGAACAAGT
ATTAAACATTACAACTCCAGAACAATTAAAAGAAGTACAAACTTATATCGAATTAGCAAAAAATAAATCAGAGATTGATC
GTAAAGATGAATCAAAACCAAAAACTGGAGTTTTCACTGGAAGCTATGCAACTAATCCACATACTAATGAATTAGTTCAA
GTTTGAGTTAGTGATTATGTATTAGCAAATTATGGAACTGGTGCTGTTATGGCAGTCCCTGCTCACGATAAAAGAGATTG
AGAATTTGCAACTAAATTCAACTTAGAGAAAAAATTTGTTATTGAAAACAAAACTGATGAAAAAGCTTTTGTTGGAGAAG
GTAAAATTATCAACTCAGATATTTTAAATGGGATGGATAAAAAACAAGCTATTCAAACAATGACAAAAATTGCTATTGAA
CAAGGTTGAGGAAGAGAACAAACAAACTATAAATTAAGAGATTGATTATTCTCACGTCAAAGATTTTATGGTGAACCTTT
CCCAGTCTTATATGGACCAAATCAAGAAATAACATTAATTGAGGATTTACCAGTTGAATTACCAAGAATTAAAAATATTA
AACCAAGTGGTACTGGAGAATCACCATTAGCCAATGTTGAAGAATGAGTTAATGTAGAAATTGATGGTGTTAAATATAGA
AGAGAAACAAACACAATGCCTCAATCTGCAGGTTCAAGTTGATATTACTTAGCTTATATTTTAGCTGATGGAGAAAATGA
ATTTATTGACATTGATAGTGCAGAAGCTAAAAAACGTTTTGAAAAATGAATGCCAGTTGATTTATATGTTGGTGGACAAG
AACATGCTGTTGGTCATTTACTATACGCAAGATTTTGAAACTATGTATTATATGATCTAGGAATTACTTCAGTTAAAGAA
CCGTTTAAACAATTATTTAATCAAGGAATGATTTTAGGACCAGATGGAAGAAAAATGTCTAAATCATGAGGAAATGTAAT
TAATCCTGATGACATTGTTTCAACACATGGTGCTGATAGTTTAAGATTATATGAAATGTTCATGGGACCATTAGATGCTT
CATTACCATGAAGTGAAGATGGATTAGATTCTGCTTTAAAATGAATTCATAGAGCTTATAGAATGGTTATGACTACTGAA
TTAACTGATGTAAATGATACTAAACTAGACTTTGTTTATAATGATGTAGTTAAAAATGTAAGTGAAATGATTGAATCATT
AAAATTTAATACAGCAATATCACAATTAATGATATTTGTTAACGCAGTTTATAAACATGAAGGACCAGTATATCGTCCTT
ACATTGAAGGATTTGTAAAAATGTTAAGTATTTATGCTCCATTCATTGGAGAAGAATTATGAGAAAAACTAGGACATGCT
CCTTCAATTACAAAACAAGCTTGACCAGTTTTTGATCCAAGTAAATTAGTTTCAAACACAGTTGTTATTGCTCTACAAAT
TAATGGTAAATTAAGAGCTACTATTGAAGTAGAAAAAGGAACTATTAAAGATAAATTATTAGAATTAGCTAAGAAACAAG
AGAGCATTATTAGCTATATTAAAGATAAAGAAATCATTAAAGAGATTGCTGTAGTTGACAGAATTGTTAACATAGTTATT
AAATAA

Upstream 100 bases:

>100_bases
GACCATTAGATATCACAAGTAATAATCATTTGATTATTACTTTTTTTATTTTTTAAAAATAGTAAAATTAATAATAGTTT
ATATTTACAAGGAGTTTAAA

Downstream 100 bases:

>100_bases
GGGTGCAAAAAAACAAAAAATCAGTATTGACACTGATTTTTTTTGTTATAATATTTTGGTTAAAAGAAAGGAATAGAGAA
AATGAGAAAAACAGTTAGAT

Product: leucyl-tRNA synthetase

Products: NA

Alternate protein names: Leucine--tRNA ligase; LeuRS [H]

Number of amino acids: Translated: 801; Mature: 801

Protein sequence:

>801_residues
MEFSHKAIEKKWKKYWEENNTNKTTNTSDKKSYVLDMFPYPSGAGIHVGHVKGYTATDVFSRYKRMNGYDVLHPMGWDAF
GLPAEQYALKTGNDPIDFTLENIKTFKRQLKMMGFSYDFDKEISTANPNYYKITQWIFNQLYKKGLAENRDVEVNWCQEL
GTVLANDEIIEKDGLMVSERGEHPVTKRKMRQWVLKITEYADRLLEGLDELEWNSSIKDLQRNWIGKSTGVELDFLVNNI
KVPVFTTRIDTIYGVSYIVLAPEHEQVLNITTPEQLKEVQTYIELAKNKSEIDRKDESKPKTGVFTGSYATNPHTNELVQ
VWVSDYVLANYGTGAVMAVPAHDKRDWEFATKFNLEKKFVIENKTDEKAFVGEGKIINSDILNGMDKKQAIQTMTKIAIE
QGWGREQTNYKLRDWLFSRQRFYGEPFPVLYGPNQEITLIEDLPVELPRIKNIKPSGTGESPLANVEEWVNVEIDGVKYR
RETNTMPQSAGSSWYYLAYILADGENEFIDIDSAEAKKRFEKWMPVDLYVGGQEHAVGHLLYARFWNYVLYDLGITSVKE
PFKQLFNQGMILGPDGRKMSKSWGNVINPDDIVSTHGADSLRLYEMFMGPLDASLPWSEDGLDSALKWIHRAYRMVMTTE
LTDVNDTKLDFVYNDVVKNVSEMIESLKFNTAISQLMIFVNAVYKHEGPVYRPYIEGFVKMLSIYAPFIGEELWEKLGHA
PSITKQAWPVFDPSKLVSNTVVIALQINGKLRATIEVEKGTIKDKLLELAKKQESIISYIKDKEIIKEIAVVDRIVNIVI
K

Sequences:

>Translated_801_residues
MEFSHKAIEKK*KKY*EENNTNKTTNTSDKKSYVLDMFPYPSGAGIHVGHVKGYTATDVFSRYKRMNGYDVLHPMG*DAF
GLPAEQYALKTGNDPIDFTLENIKTFKRQLKMMGFSYDFDKEISTANPNYYKITQ*IFNQLYKKGLAENRDVEVN*CQEL
GTVLANDEIIEKDGLMVSERGEHPVTKRKMRQ*VLKITEYADRLLEGLDELE*NSSIKDLQRN*IGKSTGVELDFLVNNI
KVPVFTTRIDTIYGVSYIVLAPEHEQVLNITTPEQLKEVQTYIELAKNKSEIDRKDESKPKTGVFTGSYATNPHTNELVQ
V*VSDYVLANYGTGAVMAVPAHDKRD*EFATKFNLEKKFVIENKTDEKAFVGEGKIINSDILNGMDKKQAIQTMTKIAIE
QG*GREQTNYKLRD*LFSRQRFYGEPFPVLYGPNQEITLIEDLPVELPRIKNIKPSGTGESPLANVEE*VNVEIDGVKYR
RETNTMPQSAGSS*YYLAYILADGENEFIDIDSAEAKKRFEK*MPVDLYVGGQEHAVGHLLYARF*NYVLYDLGITSVKE
PFKQLFNQGMILGPDGRKMSKS*GNVINPDDIVSTHGADSLRLYEMFMGPLDASLP*SEDGLDSALK*IHRAYRMVMTTE
LTDVNDTKLDFVYNDVVKNVSEMIESLKFNTAISQLMIFVNAVYKHEGPVYRPYIEGFVKMLSIYAPFIGEEL*EKLGHA
PSITKQA*PVFDPSKLVSNTVVIALQINGKLRATIEVEKGTIKDKLLELAKKQESIISYIKDKEIIKEIAVVDRIVNIVI
K
>Mature_801_residues
MEFSHKAIEKK*KKY*EENNTNKTTNTSDKKSYVLDMFPYPSGAGIHVGHVKGYTATDVFSRYKRMNGYDVLHPMG*DAF
GLPAEQYALKTGNDPIDFTLENIKTFKRQLKMMGFSYDFDKEISTANPNYYKITQ*IFNQLYKKGLAENRDVEVN*CQEL
GTVLANDEIIEKDGLMVSERGEHPVTKRKMRQ*VLKITEYADRLLEGLDELE*NSSIKDLQRN*IGKSTGVELDFLVNNI
KVPVFTTRIDTIYGVSYIVLAPEHEQVLNITTPEQLKEVQTYIELAKNKSEIDRKDESKPKTGVFTGSYATNPHTNELVQ
V*VSDYVLANYGTGAVMAVPAHDKRD*EFATKFNLEKKFVIENKTDEKAFVGEGKIINSDILNGMDKKQAIQTMTKIAIE
QG*GREQTNYKLRD*LFSRQRFYGEPFPVLYGPNQEITLIEDLPVELPRIKNIKPSGTGESPLANVEE*VNVEIDGVKYR
RETNTMPQSAGSS*YYLAYILADGENEFIDIDSAEAKKRFEK*MPVDLYVGGQEHAVGHLLYARF*NYVLYDLGITSVKE
PFKQLFNQGMILGPDGRKMSKS*GNVINPDDIVSTHGADSLRLYEMFMGPLDASLP*SEDGLDSALK*IHRAYRMVMTTE
LTDVNDTKLDFVYNDVVKNVSEMIESLKFNTAISQLMIFVNAVYKHEGPVYRPYIEGFVKMLSIYAPFIGEEL*EKLGHA
PSITKQA*PVFDPSKLVSNTVVIALQINGKLRATIEVEKGTIKDKLLELAKKQESIISYIKDKEIIKEIAVVDRIVNIVI
K

Specific function: Unknown

COG id: COG0495

COG function: function code J; Leucyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI7661872, Length=870, Percent_Identity=30.2298850574713, Blast_Score=291, Evalue=2e-78,
Organism=Escherichia coli, GI1786861, Length=870, Percent_Identity=34.1379310344828, Blast_Score=396, Evalue=1e-111,
Organism=Caenorhabditis elegans, GI71997517, Length=765, Percent_Identity=27.0588235294118, Blast_Score=202, Evalue=5e-52,
Organism=Caenorhabditis elegans, GI71997510, Length=765, Percent_Identity=27.0588235294118, Blast_Score=202, Evalue=6e-52,
Organism=Saccharomyces cerevisiae, GI6323414, Length=768, Percent_Identity=32.421875, Blast_Score=291, Evalue=3e-79,
Organism=Drosophila melanogaster, GI21355409, Length=849, Percent_Identity=28.6219081272085, Blast_Score=253, Evalue=4e-67,

Paralogues:

None

Copy number: 800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001412
- InterPro:   IPR015413
- InterPro:   IPR002300
- InterPro:   IPR002302
- InterPro:   IPR014729
- InterPro:   IPR009080
- InterPro:   IPR013155
- InterPro:   IPR009008 [H]

Pfam domain/function: PF08264 Anticodon_1; PF00133 tRNA-synt_1; PF09334 tRNA-synt_1g [H]

EC number: =6.1.1.4 [H]

Molecular weight: Translated: 87782; Mature: 87782

Theoretical pI: Translated: 5.75; Mature: 5.75

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.1 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.1 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEFSHKAIEKKKKYEENNTNKTTNTSDKKSYVLDMFPYPSGAGIHVGHVKGYTATDVFSR
CCCCHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEECCCCCCCCEEEECCCCCHHHHHHHH
YKRMNGYDVLHPMGDAFGLPAEQYALKTGNDPIDFTLENIKTFKRQLKMMGFSYDFDKEI
HHHCCCCCCCCCCCHHCCCCHHHHHHCCCCCCCEEEHHHHHHHHHHHHHHCCCCCCCCCC
STANPNYYKITQIFNQLYKKGLAENRDVEVNCQELGTVLANDEIIEKDGLMVSERGEHPV
CCCCCCEEEHHHHHHHHHHHCCCCCCCCEEEHHHHHHHHCCCCEECCCCCEEECCCCCCH
TKRKMRQVLKITEYADRLLEGLDELENSSIKDLQRNIGKSTGVELDFLVNNIKVPVFTTR
HHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCEEEEEECCEEEEEEEEE
IDTIYGVSYIVLAPEHEQVLNITTPEQLKEVQTYIELAKNKSEIDRKDESKPKTGVFTGS
HHHHHCCEEEEECCCCCCEEECCCHHHHHHHHHHHHHHHCHHHHCCCCCCCCCCCEEECC
YATNPHTNELVQVVSDYVLANYGTGAVMAVPAHDKRDEFATKFNLEKKFVIENKTDEKAF
CCCCCCHHHHHHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHCCCEEEEEECCCCCCEE
VGEGKIINSDILNGMDKKQAIQTMTKIAIEQGGREQTNYKLRDLFSRQRFYGEPFPVLYG
CCCCCEECHHHHCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCCCEEEC
PNQEITLIEDLPVELPRIKNIKPSGTGESPLANVEEVNVEIDGVKYRRETNTMPQSAGSS
CCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEECCEEEECCCCCCCCCCCCC
YYLAYILADGENEFIDIDSAEAKKRFEKMPVDLYVGGQEHAVGHLLYARFNYVLYDLGIT
EEEEEEEECCCCCEEECCCHHHHHHHHHCCEEEEECCCHHHHHHHHHHHHHHHHEECCCH
SVKEPFKQLFNQGMILGPDGRKMSKSGNVINPDDIVSTHGADSLRLYEMFMGPLDASLPS
HHHHHHHHHHHCCEEECCCCCCCCCCCCCCCHHHHHHCCCCCHHHHHHHHHCCCCCCCCC
EDGLDSALKIHRAYRMVMTTELTDVNDTKLDFVYNDVVKNVSEMIESLKFNTAISQLMIF
CCCHHHHHHHHHHHHHHHEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VNAVYKHEGPVYRPYIEGFVKMLSIYAPFIGEELEKLGHAPSITKQAPVFDPSKLVSNTV
HHHHHHCCCCEECHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHCCEE
VIALQINGKLRATIEVEKGTIKDKLLELAKKQESIISYIKDKEIIKEIAVVDRIVNIVIK
EEEEEECCEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MEFSHKAIEKKKKYEENNTNKTTNTSDKKSYVLDMFPYPSGAGIHVGHVKGYTATDVFSR
CCCCHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEECCCCCCCCEEEECCCCCHHHHHHHH
YKRMNGYDVLHPMGDAFGLPAEQYALKTGNDPIDFTLENIKTFKRQLKMMGFSYDFDKEI
HHHCCCCCCCCCCCHHCCCCHHHHHHCCCCCCCEEEHHHHHHHHHHHHHHCCCCCCCCCC
STANPNYYKITQIFNQLYKKGLAENRDVEVNCQELGTVLANDEIIEKDGLMVSERGEHPV
CCCCCCEEEHHHHHHHHHHHCCCCCCCCEEEHHHHHHHHCCCCEECCCCCEEECCCCCCH
TKRKMRQVLKITEYADRLLEGLDELENSSIKDLQRNIGKSTGVELDFLVNNIKVPVFTTR
HHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCEEEEEECCEEEEEEEEE
IDTIYGVSYIVLAPEHEQVLNITTPEQLKEVQTYIELAKNKSEIDRKDESKPKTGVFTGS
HHHHHCCEEEEECCCCCCEEECCCHHHHHHHHHHHHHHHCHHHHCCCCCCCCCCCEEECC
YATNPHTNELVQVVSDYVLANYGTGAVMAVPAHDKRDEFATKFNLEKKFVIENKTDEKAF
CCCCCCHHHHHHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHCCCEEEEEECCCCCCEE
VGEGKIINSDILNGMDKKQAIQTMTKIAIEQGGREQTNYKLRDLFSRQRFYGEPFPVLYG
CCCCCEECHHHHCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCCCEEEC
PNQEITLIEDLPVELPRIKNIKPSGTGESPLANVEEVNVEIDGVKYRRETNTMPQSAGSS
CCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEECCEEEECCCCCCCCCCCCC
YYLAYILADGENEFIDIDSAEAKKRFEKMPVDLYVGGQEHAVGHLLYARFNYVLYDLGIT
EEEEEEEECCCCCEEECCCHHHHHHHHHCCEEEEECCCHHHHHHHHHHHHHHHHEECCCH
SVKEPFKQLFNQGMILGPDGRKMSKSGNVINPDDIVSTHGADSLRLYEMFMGPLDASLPS
HHHHHHHHHHHCCEEECCCCCCCCCCCCCCCHHHHHHCCCCCHHHHHHHHHCCCCCCCCC
EDGLDSALKIHRAYRMVMTTELTDVNDTKLDFVYNDVVKNVSEMIESLKFNTAISQLMIF
CCCHHHHHHHHHHHHHHHEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VNAVYKHEGPVYRPYIEGFVKMLSIYAPFIGEELEKLGHAPSITKQAPVFDPSKLVSNTV
HHHHHHCCCCEECHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHCCEE
VIALQINGKLRATIEVEKGTIKDKLLELAKKQESIISYIKDKEIIKEIAVVDRIVNIVIK
EEEEEECCEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA