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The map label for this gene is eno [H]

Identifier: 50365285

GI number: 50365285

Start: 551920

End: 553281

Strand: Reverse

Name: eno [H]

Synonym: Mfl468

Alternate gene names: 50365285

Gene position: 553281-551920 (Counterclockwise)

Preceding gene: 50365287

Following gene: 50365284

Centisome position: 69.75

GC content: 34.21

Gene sequence:

>1362_bases
ATGTCAAGAATTGAGAAAATTATTGCACGTGAAGTATTAGACTCACGTGGAACTCCAACTGTTGAAGTTGAATTATGAAC
TGAATTTGGTGGTTATGGAATTGCTAAAGCACCATCAGGAGCATCAACTGGAGAAAACGAAGCTTTAGAACTAAGAGATG
GAGACAAAGCACGTTACAACGGTAAAGGTGTTTTAAAAGCAGTTGCTAATGTAAACGATAAAATCGCACCAGCTTTAATT
GGACACGATGTTCAAGACCAATTAGGTTTAGACAGAGTAATGATTAAATTAGATGGAACTGAATTTAAGAAAAAATTAGG
AGCAAACGGTATGTTAGCTGTTTCATTAGCTGCAGCTCACGCAGCAGCAAGTGAATTAGAAGTTCCTTTATACAGATACA
TTGGTGGAGTTCAAGCAAAACGTTTACCTGTTCCAATGTTAAATGTTATTAATGGTGGAGAACATGCTGATTCAGCAATC
GATTTCCAAGAATTCATGATTATGCCAGTTGGAGCACCAACATTTAAAGAAGCATTAAGATGATCATCAGAAACTTTCCA
AGCATTAAAATCATTATTACATGATAAAGGTGATATTACTGCTGTTGGAGACGAAGGTGGATTTGCACCTCATTTCTCAT
GAGCATATGCTAAACAAGACTTAGCTTCATTTAAAGCTAAAACACCTGCTGAAATTGCATTAGACTTATTAGTTGAAGCT
ATTACAAAAGCTGGTTACAAAGTTGGTAAAGATGGAATCATGATCGCAATGGACTGTGCTTCATCAGAATTATACTTTGA
AGACAAAAAATACCACTTCAAAAAAATTGAAAAAGTTACTGGTCAAGAATGAGCATTCACTACAGAAGAAATGATTGCTT
ACTTAGAAAAATTAGTAAACAACTACCCAATTATTTCAATCGAAGATGGATTAAGTGAAAAAGACTGAGATGGATTTGTT
CAATTAACTGAAAAAATTGGAGACAGAGTTCAAATCGTTGGAGATGACTTATTTACAACAAACCCAAGATTTATTAAAGA
AGGAATCAGCAAAGATGCTGCTAACTCAACTTTAATTAAATTAAACCAAATTGGAACTTTATCAGAAACTGTTGAAGCTA
TTACTATGACTCAAAAAGCTGGTTGAACTGCAGTTGTTTCACACCGTTCAGGAGAAACAGAAGACGCAACAATCGCTGAC
TTAGCTGTAGCATTTAATGCAGGTCAAATCAAAACAGGATCAATGTCACGTTCAGATAGAATTGCAAAATACAACAGATT
ATTACAAATCGAAGACCAATTAGGTGAAGATGCAATTTATGATGGATATGCAACATTCTATAACTTAAAAATTAATAAAT
AA

Upstream 100 bases:

>100_bases
TGATATCACATTTTAAGAGATAATTTCTATTTAATTTTTTTTAAAAAGAGAATATAATATAGGAGTTAAATACATAATTT
AAATAAAGAGAGGAAATTAT

Downstream 100 bases:

>100_bases
TTTTAAATAATTATTCAAACTAAGCTTTATGCTTAGTTTTTTTATATTAAAATTAGATTTATAAGAATAAATAATGGAGG
TATTATGTTTTATACAATTG

Product: phosphopyruvate hydratase

Products: NA

Alternate protein names: 2-phospho-D-glycerate hydro-lyase; 2-phosphoglycerate dehydratase [H]

Number of amino acids: Translated: 453; Mature: 452

Protein sequence:

>453_residues
MSRIEKIIAREVLDSRGTPTVEVELWTEFGGYGIAKAPSGASTGENEALELRDGDKARYNGKGVLKAVANVNDKIAPALI
GHDVQDQLGLDRVMIKLDGTEFKKKLGANGMLAVSLAAAHAAASELEVPLYRYIGGVQAKRLPVPMLNVINGGEHADSAI
DFQEFMIMPVGAPTFKEALRWSSETFQALKSLLHDKGDITAVGDEGGFAPHFSWAYAKQDLASFKAKTPAEIALDLLVEA
ITKAGYKVGKDGIMIAMDCASSELYFEDKKYHFKKIEKVTGQEWAFTTEEMIAYLEKLVNNYPIISIEDGLSEKDWDGFV
QLTEKIGDRVQIVGDDLFTTNPRFIKEGISKDAANSTLIKLNQIGTLSETVEAITMTQKAGWTAVVSHRSGETEDATIAD
LAVAFNAGQIKTGSMSRSDRIAKYNRLLQIEDQLGEDAIYDGYATFYNLKINK

Sequences:

>Translated_453_residues
MSRIEKIIAREVLDSRGTPTVEVEL*TEFGGYGIAKAPSGASTGENEALELRDGDKARYNGKGVLKAVANVNDKIAPALI
GHDVQDQLGLDRVMIKLDGTEFKKKLGANGMLAVSLAAAHAAASELEVPLYRYIGGVQAKRLPVPMLNVINGGEHADSAI
DFQEFMIMPVGAPTFKEALR*SSETFQALKSLLHDKGDITAVGDEGGFAPHFS*AYAKQDLASFKAKTPAEIALDLLVEA
ITKAGYKVGKDGIMIAMDCASSELYFEDKKYHFKKIEKVTGQE*AFTTEEMIAYLEKLVNNYPIISIEDGLSEKD*DGFV
QLTEKIGDRVQIVGDDLFTTNPRFIKEGISKDAANSTLIKLNQIGTLSETVEAITMTQKAG*TAVVSHRSGETEDATIAD
LAVAFNAGQIKTGSMSRSDRIAKYNRLLQIEDQLGEDAIYDGYATFYNLKINK
>Mature_452_residues
SRIEKIIAREVLDSRGTPTVEVEL*TEFGGYGIAKAPSGASTGENEALELRDGDKARYNGKGVLKAVANVNDKIAPALIG
HDVQDQLGLDRVMIKLDGTEFKKKLGANGMLAVSLAAAHAAASELEVPLYRYIGGVQAKRLPVPMLNVINGGEHADSAID
FQEFMIMPVGAPTFKEALR*SSETFQALKSLLHDKGDITAVGDEGGFAPHFS*AYAKQDLASFKAKTPAEIALDLLVEAI
TKAGYKVGKDGIMIAMDCASSELYFEDKKYHFKKIEKVTGQE*AFTTEEMIAYLEKLVNNYPIISIEDGLSEKD*DGFVQ
LTEKIGDRVQIVGDDLFTTNPRFIKEGISKDAANSTLIKLNQIGTLSETVEAITMTQKAG*TAVVSHRSGETEDATIADL
AVAFNAGQIKTGSMSRSDRIAKYNRLLQIEDQLGEDAIYDGYATFYNLKINK

Specific function: Catalyzes the reversible conversion of 2- phosphoglycerate into phosphoenolpyruvate. It is essential for the degradation of carbohydrates via glycolysis [H]

COG id: COG0148

COG function: function code G; Enolase

Gene ontology:

Cell location: Cytoplasm. Secreted. Cell surface. Note=Fractions of enolase are present in both the cytoplasm and on the cell surface. The export of enolase possibly depends on the covalent binding to the substrate; once secreted, it remains attached to the bacterial ce

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enolase family [H]

Homologues:

Organism=Homo sapiens, GI5803011, Length=449, Percent_Identity=48.5523385300668, Blast_Score=377, Evalue=1e-105,
Organism=Homo sapiens, GI301897477, Length=442, Percent_Identity=48.1900452488688, Blast_Score=367, Evalue=1e-101,
Organism=Homo sapiens, GI301897469, Length=442, Percent_Identity=48.1900452488688, Blast_Score=367, Evalue=1e-101,
Organism=Homo sapiens, GI4503571, Length=443, Percent_Identity=46.27539503386, Blast_Score=351, Evalue=7e-97,
Organism=Homo sapiens, GI301897479, Length=440, Percent_Identity=43.1818181818182, Blast_Score=308, Evalue=5e-84,
Organism=Homo sapiens, GI169201331, Length=344, Percent_Identity=25.5813953488372, Blast_Score=83, Evalue=5e-16,
Organism=Homo sapiens, GI169201757, Length=344, Percent_Identity=25.5813953488372, Blast_Score=83, Evalue=5e-16,
Organism=Homo sapiens, GI239744207, Length=344, Percent_Identity=25.5813953488372, Blast_Score=83, Evalue=5e-16,
Organism=Escherichia coli, GI1789141, Length=444, Percent_Identity=59.009009009009, Blast_Score=468, Evalue=1e-133,
Organism=Caenorhabditis elegans, GI17536383, Length=453, Percent_Identity=48.7858719646799, Blast_Score=364, Evalue=1e-101,
Organism=Caenorhabditis elegans, GI71995829, Length=453, Percent_Identity=48.7858719646799, Blast_Score=364, Evalue=1e-101,
Organism=Caenorhabditis elegans, GI32563855, Length=203, Percent_Identity=42.3645320197044, Blast_Score=155, Evalue=3e-38,
Organism=Saccharomyces cerevisiae, GI6321693, Length=447, Percent_Identity=49.6644295302013, Blast_Score=360, Evalue=1e-100,
Organism=Saccharomyces cerevisiae, GI6323985, Length=447, Percent_Identity=46.3087248322148, Blast_Score=345, Evalue=9e-96,
Organism=Saccharomyces cerevisiae, GI6324974, Length=447, Percent_Identity=46.3087248322148, Blast_Score=345, Evalue=9e-96,
Organism=Saccharomyces cerevisiae, GI6324969, Length=447, Percent_Identity=46.3087248322148, Blast_Score=345, Evalue=9e-96,
Organism=Saccharomyces cerevisiae, GI6321968, Length=448, Percent_Identity=48.4375, Blast_Score=323, Evalue=4e-89,
Organism=Drosophila melanogaster, GI24580918, Length=444, Percent_Identity=46.8468468468468, Blast_Score=343, Evalue=2e-94,
Organism=Drosophila melanogaster, GI24580916, Length=444, Percent_Identity=46.8468468468468, Blast_Score=343, Evalue=2e-94,
Organism=Drosophila melanogaster, GI24580920, Length=444, Percent_Identity=46.8468468468468, Blast_Score=343, Evalue=2e-94,
Organism=Drosophila melanogaster, GI24580914, Length=444, Percent_Identity=46.8468468468468, Blast_Score=343, Evalue=2e-94,
Organism=Drosophila melanogaster, GI281360527, Length=444, Percent_Identity=46.8468468468468, Blast_Score=342, Evalue=4e-94,
Organism=Drosophila melanogaster, GI17137654, Length=444, Percent_Identity=46.8468468468468, Blast_Score=342, Evalue=4e-94,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1660 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 20 Molecules/Cell In: Stationary Phase,

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000941
- InterPro:   IPR020810
- InterPro:   IPR020809
- InterPro:   IPR020811 [H]

Pfam domain/function: PF00113 Enolase_C; PF03952 Enolase_N [H]

EC number: =4.2.1.11 [H]

Molecular weight: Translated: 48325; Mature: 48194

Theoretical pI: Translated: 4.87; Mature: 4.87

Prosite motif: PS00164 ENOLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRIEKIIAREVLDSRGTPTVEVELTEFGGYGIAKAPSGASTGENEALELRDGDKARYNG
CCHHHHHHHHHHHHCCCCCEEEEEEEECCCCCCCCCCCCCCCCCCCEEEECCCCCCEECC
KGVLKAVANVNDKIAPALIGHDVQDQLGLDRVMIKLDGTEFKKKLGANGMLAVSLAAAHA
CHHHHHHHCCCCCHHHHHHCCCCHHHHCCEEEEEEECCHHHHHHHCCCCEEEEEHHHHHH
AASELEVPLYRYIGGVQAKRLPVPMLNVINGGEHADSAIDFQEFMIMPVGAPTFKEALRS
HHHHCCCHHHHHHCCCCCCCCCCCHHHHCCCCCCCCCCCCHHHEEEEECCCHHHHHHHCC
SETFQALKSLLHDKGDITAVGDEGGFAPHFSAYAKQDLASFKAKTPAEIALDLLVEAITK
CHHHHHHHHHHCCCCCEEEECCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
AGYKVGKDGIMIAMDCASSELYFEDKKYHFKKIEKVTGQEAFTTEEMIAYLEKLVNNYPI
CCCEECCCCEEEEEECCCCCEEECCCHHHHHHHHHHCCCHHCCHHHHHHHHHHHHCCCCE
ISIEDGLSEKDDGFVQLTEKIGDRVQIVGDDLFTTNPRFIKEGISKDAANSTLIKLNQIG
EEECCCCCCCCCCHHHHHHHHCCEEEEEECCCCCCCHHHHHHHCCCCCCCCEEEEEECCC
TLSETVEAITMTQKAGTAVVSHRSGETEDATIADLAVAFNAGQIKTGSMSRSDRIAKYNR
CHHHHHHHHHHHHHCCCEEEECCCCCCCCCHHHHHHEEECCCCEECCCCCCHHHHHHHHH
LLQIEDQLGEDAIYDGYATFYNLKINK
HEEEHHHCCCCHHCCCEEEEEEEEECC
>Mature Secondary Structure 
SRIEKIIAREVLDSRGTPTVEVELTEFGGYGIAKAPSGASTGENEALELRDGDKARYNG
CHHHHHHHHHHHHCCCCCEEEEEEEECCCCCCCCCCCCCCCCCCCEEEECCCCCCEECC
KGVLKAVANVNDKIAPALIGHDVQDQLGLDRVMIKLDGTEFKKKLGANGMLAVSLAAAHA
CHHHHHHHCCCCCHHHHHHCCCCHHHHCCEEEEEEECCHHHHHHHCCCCEEEEEHHHHHH
AASELEVPLYRYIGGVQAKRLPVPMLNVINGGEHADSAIDFQEFMIMPVGAPTFKEALRS
HHHHCCCHHHHHHCCCCCCCCCCCHHHHCCCCCCCCCCCCHHHEEEEECCCHHHHHHHCC
SETFQALKSLLHDKGDITAVGDEGGFAPHFSAYAKQDLASFKAKTPAEIALDLLVEAITK
CHHHHHHHHHHCCCCCEEEECCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
AGYKVGKDGIMIAMDCASSELYFEDKKYHFKKIEKVTGQEAFTTEEMIAYLEKLVNNYPI
CCCEECCCCEEEEEECCCCCEEECCCHHHHHHHHHHCCCHHCCHHHHHHHHHHHHCCCCE
ISIEDGLSEKDDGFVQLTEKIGDRVQIVGDDLFTTNPRFIKEGISKDAANSTLIKLNQIG
EEECCCCCCCCCCHHHHHHHHCCEEEEEECCCCCCCHHHHHHHCCCCCCCCEEEEEECCC
TLSETVEAITMTQKAGTAVVSHRSGETEDATIADLAVAFNAGQIKTGSMSRSDRIAKYNR
CHHHHHHHHHHHHHCCCEEEECCCCCCCCCHHHHHHEEECCCCEECCCCCCHHHHHHHHH
LLQIEDQLGEDAIYDGYATFYNLKINK
HEEEHHHCCCCHHCCCEEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA