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The map label for this gene is tilS/hprT [H]

Identifier: 50365280

GI number: 50365280

Start: 548823

End: 549392

Strand: Reverse

Name: tilS/hprT [H]

Synonym: Mfl463

Alternate gene names: 50365280

Gene position: 549392-548823 (Counterclockwise)

Preceding gene: 50365281

Following gene: 50365279

Centisome position: 69.26

GC content: 26.49

Gene sequence:

>570_bases
ATGAAATTACACCCATTAGTAAAAAATGTATTATTTACTGAAGAACAAATAAAAGAAAGAACAATTGAAGTAGCAAAAGA
AATTAAATCATTTTACGAACAAAATGATAAAGAAGACCAATCATTATTGGTTATAGGATTATTAAAAGGATGTATTCCTT
TTTATCAAACATTCTGTATGAATTTTGACTTTTTAATTGATATGGATTTTATGGTTGTAAGTTCATATTTGGGTGGAACA
AAAAGTTCTGGAGAACCCAAAATTAATTTAGACGTTAACATGTCTGTTAAAGATCGCAATATATTAATAGTTGAAGACAT
TGTTGAATCTGGTCTAACACTAGAGTTTGTACAAAATTACTTATTAAACAAAGGTGCAAAATCAGTTAAAATTTTAACTA
TGTTAGATAAACCAACTGAACGCAAAGTAGATATTAAACCAGATTGAACTTGTTTTTCAATTGCAAAACACTTTGTAATA
GGCTATGGTTTAGATTATCAAGAAAAATTAAGAAACTTACCTTATGTAGCTGTTTGTGATACTGATAAATTAGCTGATTG
AAAATGATAA

Upstream 100 bases:

>100_bases
ATATTTAGAAAAAATTAAGAAATAATTGTTAATACCTATTGATTAAGAAATCAATAATAGGTATTATTTTTATATTACAA
AATTTAAACAAAGGAGCACA

Downstream 100 bases:

>100_bases
AATATTTATTAAACTATATATAAATAATATTAAAGAAAGCATAAATGCTTTCTTTTTTTTGCTTTTTTGATCTTACTGGC
TGATTAACATTTAAGTGTTT

Product: hypoxanthine-guanine phosphoribosyltransferase

Products: NA

Alternate protein names: tRNA(Ile)-lysidine synthase; tRNA(Ile)-2-lysyl-cytidine synthase; tRNA(Ile)-lysidine synthetase; Hypoxanthine-guanine phosphoribosyltransferase; HGPRT; HGPRTase [H]

Number of amino acids: Translated: 189; Mature: 189

Protein sequence:

>189_residues
MKLHPLVKNVLFTEEQIKERTIEVAKEIKSFYEQNDKEDQSLLVIGLLKGCIPFYQTFCMNFDFLIDMDFMVVSSYLGGT
KSSGEPKINLDVNMSVKDRNILIVEDIVESGLTLEFVQNYLLNKGAKSVKILTMLDKPTERKVDIKPDWTCFSIAKHFVI
GYGLDYQEKLRNLPYVAVCDTDKLADWKW

Sequences:

>Translated_189_residues
MKLHPLVKNVLFTEEQIKERTIEVAKEIKSFYEQNDKEDQSLLVIGLLKGCIPFYQTFCMNFDFLIDMDFMVVSSYLGGT
KSSGEPKINLDVNMSVKDRNILIVEDIVESGLTLEFVQNYLLNKGAKSVKILTMLDKPTERKVDIKPD*TCFSIAKHFVI
GYGLDYQEKLRNLPYVAVCDTDKLAD*K*
>Mature_189_residues
MKLHPLVKNVLFTEEQIKERTIEVAKEIKSFYEQNDKEDQSLLVIGLLKGCIPFYQTFCMNFDFLIDMDFMVVSSYLGGT
KSSGEPKINLDVNMSVKDRNILIVEDIVESGLTLEFVQNYLLNKGAKSVKILTMLDKPTERKVDIKPD*TCFSIAKHFVI
GYGLDYQEKLRNLPYVAVCDTDKLAD*K*

Specific function: Ligates lysine onto the cytidine present at position 34 of the AUA codon-specific tRNA(Ile) that contains the anticodon CAU, in an ATP-dependent manner. Cytidine is converted to lysidine, thus changing the amino acid specificity of the tRNA from methionin

COG id: COG0634

COG function: function code F; Hypoxanthine-guanine phosphoribosyltransferase

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the purine/pyrimidine phosphoribosyltransferase family [H]

Homologues:

Organism=Homo sapiens, GI4504483, Length=182, Percent_Identity=30.7692307692308, Blast_Score=70, Evalue=1e-12,
Organism=Escherichia coli, GI87081689, Length=169, Percent_Identity=35.5029585798817, Blast_Score=115, Evalue=1e-27,
Organism=Caenorhabditis elegans, GI17510817, Length=183, Percent_Identity=27.3224043715847, Blast_Score=64, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005904
- InterPro:   IPR012094
- InterPro:   IPR012796
- InterPro:   IPR012795
- InterPro:   IPR020825
- InterPro:   IPR000836
- InterPro:   IPR014729
- InterPro:   IPR011063 [H]

Pfam domain/function: PF01171 ATP_bind_3; PF00156 Pribosyltran; PF11734 TilS_C [H]

EC number: =2.4.2.8 [H]

Molecular weight: Translated: 21220; Mature: 21220

Theoretical pI: Translated: 5.11; Mature: 5.11

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLHPLVKNVLFTEEQIKERTIEVAKEIKSFYEQNDKEDQSLLVIGLLKGCIPFYQTFCM
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHC
NFDFLIDMDFMVVSSYLGGTKSSGEPKINLDVNMSVKDRNILIVEDIVESGLTLEFVQNY
CCHHEEEHHHHHHHHHHCCCCCCCCCEEEEEEEEEECCCCEEEEHHHHHCCCCHHHHHHH
LLNKGAKSVKILTMLDKPTERKVDIKPDTCFSIAKHFVIGYGLDYQEKLRNLPYVAVCDT
HHCCCCCCEEEEEEECCCCCCEEECCCCHHHHHHHHHHHHCCCCHHHHHHCCCEEEEECC
DKLADK
HHCCCC
>Mature Secondary Structure
MKLHPLVKNVLFTEEQIKERTIEVAKEIKSFYEQNDKEDQSLLVIGLLKGCIPFYQTFCM
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHC
NFDFLIDMDFMVVSSYLGGTKSSGEPKINLDVNMSVKDRNILIVEDIVESGLTLEFVQNY
CCHHEEEHHHHHHHHHHCCCCCCCCCEEEEEEEEEECCCCEEEEHHHHHCCCCHHHHHHH
LLNKGAKSVKILTMLDKPTERKVDIKPDTCFSIAKHFVIGYGLDYQEKLRNLPYVAVCDT
HHCCCCCCEEEEEEECCCCCCEEECCCCHHHHHHHHHHHHCCCCHHHHHHCCCEEEEECC
DKLADK
HHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11679669 [H]