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The map label for this gene is pbuG [H]

Identifier: 50365231

GI number: 50365231

Start: 482430

End: 483959

Strand: Reverse

Name: pbuG [H]

Synonym: Mfl413

Alternate gene names: 50365231

Gene position: 483959-482430 (Counterclockwise)

Preceding gene: 50365232

Following gene: 50365230

Centisome position: 61.01

GC content: 30.46

Gene sequence:

>1530_bases
ATGGATAATAAAAGTCTAAAACAGACTCAAGTTGAGAATGAGTCTGAATTATTTATAAATGAAACACCTGAGCAAACGGC
TCAGAGAAATAAGGAAGCTTTTGTCTTCAGCAACAATAAAGCTATCCGATCAATTGAAAAGTACTTTAAGTTTGATACTT
TAGGTGCAATTATGAAGAAAGAAATCATTGGTGGTATTACAACATTTTTATCATTGATGTACATACTTACAGTAAACCCT
GGATTAGTTTCAGGAACACCTTCAATTAATACTGGTGCTGGAAATATGAATTACTTTGGTATATTTTTTGCAACAGCATT
GGTTTCTGGTATATGTTGTATAATTATGGGTTTATTTGCTAATATACCTGTAGCAATGTCAACATCAATGGGAATGAATG
CATTGGTTAGTGTAAACATTGGAATTAGTGGTGGTTTAGGCTTTGAAGGTGCAATGATTGTTACAATGTTATCATCTGTA
GTGTTTGTAACAGTTTCACTTACGCCTTTAAGATCATTCATTATTAAATCAATTCCAAAAGGAATCGTATTAGCTATTGG
TATTGGTATTGGTTTATTTATAGCTTATGTAGGTATTTCAAGCATGGGATGATTAGCTCAAGAAAATGGAATACCAATGG
CTTCAGTAGCTACATTAAAAGATAATTACTTACCAATAATATTAGGTTCAATAACTTTATTATTAATTTTATTTTTAGCA
TTTAAAAAAATACCAGGAGCAGTAGCAATTGCTATTTTATCAATGTCAGTTATTGCAATTATATTAGCATCTACACTTCC
TAGTGATTCAAAAGCTATTTCATTATTAGGTTCAGCTGACTTAAGAAAATGAGAAGGATGAAATTATGATTTTGATGGTT
TTGCATGAAATTGAACTTCTACATTTAAGGCATTCGGTAATGCTAAAATTTGAACTTCACCAGTAACATATATTTCAATA
TTTGTTGTTATGTTAATTAATTTCTTTGATGCAACAGGAACAATGGCTGCATTTACACATCAATTAGATCAAAAAACAAA
TCAACATAAAGAAATAAGTCAAAAAGCTTTAGTTATTGATTCAATGGGAACAATGATGGCTTCAGTAACTGGAACAACAC
CTTTAGGAGTTTTTGCTGAATCAGCAGCAGGTATAGAACAAGGTGCTAAAACAGGATTAGCAGCAGTTGTTAATGGAATT
CTATTTTTATTGGCAATTATAATGTTCCCAATCTTTAAATTAATTCCTCAACCTATTACAAGTGCTGCTTGTGTATATAT
TGGAATTATGATGATTAAAGAAGCTGCTCATGTTGAATGAGATAAACCTGAATTTTTAGTTCCTACATTCTTATCAATTT
TATTTATGATTGCAACTTATGAAATTGCAAATGGTGTTGCGATGGCATTTATAGGATATTCATTCATGATGATGATAACA
GCGAAAGCTAAAAAAGTTCATCCAGCAGTTTACTGCTTGTCATTCTTATTTATTATTTACTTCATTGCTTTTGCATTCAT
ACAAATATAA

Upstream 100 bases:

>100_bases
TTTATGGTTTTCGCTATGCGTATTTTTTACGTAGACGAAAACTTTTTTTATTTTTTGCTGAGTTCTTCATTGAATTTTAT
ACATAGAAAGAATAGGCAAA

Downstream 100 bases:

>100_bases
TTTGAAATAAATTAAAATATGTTAAAATTAAATTACTCATTTGAGTAATTTTTTTGTTTAAGGAGGAAATATGAAAAAGA
AAGTTATCGTAGGTTTAAGT

Product: putative xanthine/uracil permease

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 509; Mature: 509

Protein sequence:

>509_residues
MDNKSLKQTQVENESELFINETPEQTAQRNKEAFVFSNNKAIRSIEKYFKFDTLGAIMKKEIIGGITTFLSLMYILTVNP
GLVSGTPSINTGAGNMNYFGIFFATALVSGICCIIMGLFANIPVAMSTSMGMNALVSVNIGISGGLGFEGAMIVTMLSSV
VFVTVSLTPLRSFIIKSIPKGIVLAIGIGIGLFIAYVGISSMGWLAQENGIPMASVATLKDNYLPIILGSITLLLILFLA
FKKIPGAVAIAILSMSVIAIILASTLPSDSKAISLLGSADLRKWEGWNYDFDGFAWNWTSTFKAFGNAKIWTSPVTYISI
FVVMLINFFDATGTMAAFTHQLDQKTNQHKEISQKALVIDSMGTMMASVTGTTPLGVFAESAAGIEQGAKTGLAAVVNGI
LFLLAIIMFPIFKLIPQPITSAACVYIGIMMIKEAAHVEWDKPEFLVPTFLSILFMIATYEIANGVAMAFIGYSFMMMIT
AKAKKVHPAVYCLSFLFIIYFIAFAFIQI

Sequences:

>Translated_509_residues
MDNKSLKQTQVENESELFINETPEQTAQRNKEAFVFSNNKAIRSIEKYFKFDTLGAIMKKEIIGGITTFLSLMYILTVNP
GLVSGTPSINTGAGNMNYFGIFFATALVSGICCIIMGLFANIPVAMSTSMGMNALVSVNIGISGGLGFEGAMIVTMLSSV
VFVTVSLTPLRSFIIKSIPKGIVLAIGIGIGLFIAYVGISSMG*LAQENGIPMASVATLKDNYLPIILGSITLLLILFLA
FKKIPGAVAIAILSMSVIAIILASTLPSDSKAISLLGSADLRK*EG*NYDFDGFA*N*TSTFKAFGNAKI*TSPVTYISI
FVVMLINFFDATGTMAAFTHQLDQKTNQHKEISQKALVIDSMGTMMASVTGTTPLGVFAESAAGIEQGAKTGLAAVVNGI
LFLLAIIMFPIFKLIPQPITSAACVYIGIMMIKEAAHVE*DKPEFLVPTFLSILFMIATYEIANGVAMAFIGYSFMMMIT
AKAKKVHPAVYCLSFLFIIYFIAFAFIQI
>Mature_509_residues
MDNKSLKQTQVENESELFINETPEQTAQRNKEAFVFSNNKAIRSIEKYFKFDTLGAIMKKEIIGGITTFLSLMYILTVNP
GLVSGTPSINTGAGNMNYFGIFFATALVSGICCIIMGLFANIPVAMSTSMGMNALVSVNIGISGGLGFEGAMIVTMLSSV
VFVTVSLTPLRSFIIKSIPKGIVLAIGIGIGLFIAYVGISSMG*LAQENGIPMASVATLKDNYLPIILGSITLLLILFLA
FKKIPGAVAIAILSMSVIAIILASTLPSDSKAISLLGSADLRK*EG*NYDFDGFA*N*TSTFKAFGNAKI*TSPVTYISI
FVVMLINFFDATGTMAAFTHQLDQKTNQHKEISQKALVIDSMGTMMASVTGTTPLGVFAESAAGIEQGAKTGLAAVVNGI
LFLLAIIMFPIFKLIPQPITSAACVYIGIMMIKEAAHVE*DKPEFLVPTFLSILFMIATYEIANGVAMAFIGYSFMMMIT
AKAKKVHPAVYCLSFLFIIYFIAFAFIQI

Specific function: Involved in the uptake of the purine bases hypoxanthine and guanine [H]

COG id: COG2252

COG function: function code R; Permeases

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the xanthine/uracil permease family. AzgA purine transporter (TC 2.A.1.40) subfamily [H]

Homologues:

Organism=Escherichia coli, GI1790499, Length=468, Percent_Identity=27.7777777777778, Blast_Score=182, Evalue=3e-47,
Organism=Escherichia coli, GI87082309, Length=456, Percent_Identity=27.8508771929825, Blast_Score=177, Evalue=1e-45,
Organism=Escherichia coli, GI1790150, Length=457, Percent_Identity=28.6652078774617, Blast_Score=174, Evalue=2e-44,
Organism=Escherichia coli, GI48994909, Length=425, Percent_Identity=28.9411764705882, Blast_Score=157, Evalue=1e-39,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006043 [H]

Pfam domain/function: PF00860 Xan_ur_permease [H]

EC number: NA

Molecular weight: Translated: 53606; Mature: 53606

Theoretical pI: Translated: 8.27; Mature: 8.27

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
5.1 %Met     (Translated Protein)
5.9 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
5.1 %Met     (Mature Protein)
5.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDNKSLKQTQVENESELFINETPEQTAQRNKEAFVFSNNKAIRSIEKYFKFDTLGAIMKK
CCCCCCHHHHCCCCCCEEEECCCHHHHHCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHH
EIIGGITTFLSLMYILTVNPGLVSGTPSINTGAGNMNYFGIFFATALVSGICCIIMGLFA
HHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
NIPVAMSTSMGMNALVSVNIGISGGLGFEGAMIVTMLSSVVFVTVSLTPLRSFIIKSIPK
CCCEEEECCCCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
GIVLAIGIGIGLFIAYVGISSMGLAQENGIPMASVATLKDNYLPIILGSITLLLILFLAF
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHH
KKIPGAVAIAILSMSVIAIILASTLPSDSKAISLLGSADLRKEGNYDFDGFANTSTFKAF
HHCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHCCCHHCCCCCCCCCCCCCCCHHHHC
GNAKITSPVTYISIFVVMLINFFDATGTMAAFTHQLDQKTNQHKEISQKALVIDSMGTMM
CCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ASVTGTTPLGVFAESAAGIEQGAKTGLAAVVNGILFLLAIIMFPIFKLIPQPITSAACVY
HHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHH
IGIMMIKEAAHVEDKPEFLVPTFLSILFMIATYEIANGVAMAFIGYSFMMMITAKAKKVH
HHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
PAVYCLSFLFIIYFIAFAFIQI
HHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MDNKSLKQTQVENESELFINETPEQTAQRNKEAFVFSNNKAIRSIEKYFKFDTLGAIMKK
CCCCCCHHHHCCCCCCEEEECCCHHHHHCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHH
EIIGGITTFLSLMYILTVNPGLVSGTPSINTGAGNMNYFGIFFATALVSGICCIIMGLFA
HHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
NIPVAMSTSMGMNALVSVNIGISGGLGFEGAMIVTMLSSVVFVTVSLTPLRSFIIKSIPK
CCCEEEECCCCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
GIVLAIGIGIGLFIAYVGISSMGLAQENGIPMASVATLKDNYLPIILGSITLLLILFLAF
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHH
KKIPGAVAIAILSMSVIAIILASTLPSDSKAISLLGSADLRKEGNYDFDGFANTSTFKAF
HHCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHCCCHHCCCCCCCCCCCCCCCHHHHC
GNAKITSPVTYISIFVVMLINFFDATGTMAAFTHQLDQKTNQHKEISQKALVIDSMGTMM
CCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ASVTGTTPLGVFAESAAGIEQGAKTGLAAVVNGILFLLAIIMFPIFKLIPQPITSAACVY
HHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHH
IGIMMIKEAAHVEDKPEFLVPTFLSILFMIATYEIANGVAMAFIGYSFMMMITAKAKKVH
HHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
PAVYCLSFLFIIYFIAFAFIQI
HHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8969499; 9384377; 11591660 [H]