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The map label for this gene is folA [H]

Identifier: 50365201

GI number: 50365201

Start: 443148

End: 443633

Strand: Reverse

Name: folA [H]

Synonym: Mfl383

Alternate gene names: 50365201

Gene position: 443633-443148 (Counterclockwise)

Preceding gene: 50365202

Following gene: 50365200

Centisome position: 55.93

GC content: 25.31

Gene sequence:

>486_bases
ATGATTAAAATGATTTGAGCACAAACAGAAAAAGGAGTAATTGGTAAAGACAATTCACTTCCTTGATCTATAAAAGAAGA
AATGCAACATTTTAGAACAACAACGTTAAATCAAAATGTTTTAATGGGTGGTAAAACATTTGAGTCAATGAACTTTAAAG
GGTTACCAAATCGTATCAATTATGTACTTACAAGAGATACTGAAAAGTACAAAGAACACATGTCAGAAAATGTAAATTTC
ATTAATAAAAAAGATTTTATTTTAAAAGAATTTAAAGGCAATACATCAAAAGATGTTTACATTATTGGTGGAAGCCAAAT
ATATGAATTATTCTTTGATGATTGTGATGAAATCATTAGAAGCATGATAAAAGATGATTTTGAAGGAAATGTACATATCA
AAAACTTTAACTATGATAATTTTGATAAAATAAATATAGTGGAAAAAGAACACTTTTATGTTGAATACATGAATAGGAGA
AAATAA

Upstream 100 bases:

>100_bases
TTAGAAAAAACACCAATTATTTTAAATAAAGAATTAACTAATATATTGATTTCAATTTCTCATGAAAAAAAATATTCAGT
TGGTATGGCGGTGAAGAAAA

Downstream 100 bases:

>100_bases
AATGACAAAAGAAAAAGAAGTTAAAAAACCAGTAGCTAAAAAAACTGAAACAAAACCAGCAACTAAAAAAGCAGCTACAC
CAAAATCACCAGCTAAAAAA

Product: dihydrofolate reductase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 161; Mature: 161

Protein sequence:

>161_residues
MIKMIWAQTEKGVIGKDNSLPWSIKEEMQHFRTTTLNQNVLMGGKTFESMNFKGLPNRINYVLTRDTEKYKEHMSENVNF
INKKDFILKEFKGNTSKDVYIIGGSQIYELFFDDCDEIIRSMIKDDFEGNVHIKNFNYDNFDKINIVEKEHFYVEYMNRR
K

Sequences:

>Translated_161_residues
MIKMI*AQTEKGVIGKDNSLP*SIKEEMQHFRTTTLNQNVLMGGKTFESMNFKGLPNRINYVLTRDTEKYKEHMSENVNF
INKKDFILKEFKGNTSKDVYIIGGSQIYELFFDDCDEIIRSMIKDDFEGNVHIKNFNYDNFDKINIVEKEHFYVEYMNRR
K
>Mature_161_residues
MIKMI*AQTEKGVIGKDNSLP*SIKEEMQHFRTTTLNQNVLMGGKTFESMNFKGLPNRINYVLTRDTEKYKEHMSENVNF
INKKDFILKEFKGNTSKDVYIIGGSQIYELFFDDCDEIIRSMIKDDFEGNVHIKNFNYDNFDKINIVEKEHFYVEYMNRR
K

Specific function: Key enzyme in folate metabolism. Catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis [H]

COG id: COG0262

COG function: function code H; Dihydrofolate reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 DHFR (dihydrofolate reductase) domain [H]

Homologues:

Organism=Caenorhabditis elegans, GI17505899, Length=156, Percent_Identity=31.4102564102564, Blast_Score=65, Evalue=1e-11,

Paralogues:

None

Copy number: 640 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 10,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012259
- InterPro:   IPR017925
- InterPro:   IPR001796 [H]

Pfam domain/function: PF00186 DHFR_1 [H]

EC number: =1.5.1.3 [H]

Molecular weight: Translated: 18841; Mature: 18841

Theoretical pI: Translated: 6.96; Mature: 6.96

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
5.0 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
5.0 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIKMIAQTEKGVIGKDNSLPSIKEEMQHFRTTTLNQNVLMGGKTFESMNFKGLPNRINYV
CCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCEEECCCCCCCCCCCCCCCCCEEE
LTRDTEKYKEHMSENVNFINKKDFILKEFKGNTSKDVYIIGGSQIYELFFDDCDEIIRSM
EECCHHHHHHHHHCCCCCCCCCHHHHHHCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHH
IKDDFEGNVHIKNFNYDNFDKINIVEKEHFYVEYMNRRK
HHCCCCCCEEEEECCCCCCCEEEEEECHHHHHHHHHCCC
>Mature Secondary Structure
MIKMIAQTEKGVIGKDNSLPSIKEEMQHFRTTTLNQNVLMGGKTFESMNFKGLPNRINYV
CCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCEEECCCCCCCCCCCCCCCCCEEE
LTRDTEKYKEHMSENVNFINKKDFILKEFKGNTSKDVYIIGGSQIYELFFDDCDEIIRSM
EECCHHHHHHHHHCCCCCCCCCHHHHHHCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHH
IKDDFEGNVHIKNFNYDNFDKINIVEKEHFYVEYMNRRK
HHCCCCCCEEEEECCCCCCCEEEEEECHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7569993 [H]