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The map label for this gene is ybbF [H]

Identifier: 50365128

GI number: 50365128

Start: 361622

End: 364171

Strand: Direct

Name: ybbF [H]

Synonym: Mfl312

Alternate gene names: 50365128

Gene position: 361622-364171 (Clockwise)

Preceding gene: 50365127

Following gene: 50365129

Centisome position: 45.59

GC content: 26.63

Gene sequence:

>2550_bases
ATGAAAAATAAAAAAATTATCATTTATGCTCCTGTAAATGGAGTTATAGGTAAAATTAAAGACTTAAAAGATGGTGTTTT
TTCAGAAGGAATGCTTGGTGAAGGGGTTTATATAATTCCTGAATCAAAAGAATTTTATTCACCTATTGATAAAGGTGAGT
TAAAACTTGTTACAGCTACTAAGCACGCTTATTATTTTGAACATGAAAATGGTCCTAATATTCTTATGCATATTAGTTTA
GATACTGAAAAACATCAAGGTAAACCCTTTAAAACATTATCAAATGTTGGAGATAAGTTGACTTTAGCAACTAAAATTGT
TTCAGTTGATTTACAAATGTGTAAAAAACAAGATGTTAATGTTGCAACACCTATATTAATTGACACAAAAGAGTTTTCTG
AATGAAAATTTAAACCGTTAGACTTAAAAGGAAAAGTTAAAAGAGGCCAACCTATAGGAGAATTTGTTAAAGTTGAAGAG
AAAAAAATAAATGAAGAAAGTAATGATCTAACTACTTTTGAATTTAAAAATAAATATGAAAAATCTGCAGAAAGTATATA
TGAATACATAGGGACTGAGTCTAATTATTCAAAAGTTTATAATTGTATGACTAGACTTCGTTTTTATGTAAAAGATAAAC
AAATTGTTAACACAGAAGCAATAAAAAAAATGCCAATAGTAAAAGGTGTTAATTGAAATGGAGAAGAAATTCAAATAATT
ATTGGAGGAGAAGTTCAAAAAGTTAAAGATGCTTTTGAAAATTATGTTTCAAATAAAAATATATCAAAAACTTCTAAAAA
ACAAGAAGGTTTTGAACAGAAAATAAAACCTAAAAAACCAATGAAAGAAGCTATTTTAGGTGCAATCTCTGGTATTATGG
TTCCAACTTTACCTGTATTTATAACAGTTGGTATGTTAATGGGACTTAAAGCAATACTTGTGCAGGCAGGAGCAATGCCT
GAAATAGTAACAGGAATAGCAAAAGAAGGACAAGTTTCTCTAACAGATGCAGATGTTTTTTCAGCAATATTTTTTATAAT
AACTGAAATTGCTTTTAAATTTCTAGGGATATTTATTGGTTATAACACCATGAAATATTTGGGTGGTAATACAATAATGT
CAATATTAATAGGATTAGCATTAGCTAATCCATACATATGACAAGGAACTTCATGACCTTTATTTACAATTGGTTCAACT
ACAATTAAGGTTCAAGCATTTACAAGTTCTATAATGCCACATATAGCTGCAAATATAATATTCTTTTATCTAGACAAATG
AATAAAAACGTGAATGCCTTCTGTAGTAGATATAATTTTTAGACCAATGATCGCATTTTTAACAACTATGTTATTAGCAT
TCTTTATTGTTGGTCCATCTTTATTTTTAGTTGAACAAGGACTTGCAGTTATTGTTGGATGATTAGGATCAATTCCGTTT
GGAATTGGAGTAGCTATATATGCTATATTATGGCAACCTTTTGTTATATCAGGTATGCATATAGCAATAGCTATGCCTTT
ATCAATTGGATTAATGCAGGGTACACCTCAAGTTATTTCACCTGCTATTATGTATGGTTGTTACGGTCAACTTGGAGCAA
TGATTGGGGTTATGATAATGACTAAAAACTCACAACTAAAAGCAACTTGTATAGCTAATTTAACACCTGGTGTTATTGCT
ATTACTGAACCTATAATTTATGGAATTACTTTACCAAAAGGAAGACCTTTTATAGCAGGTTGTTTAGGCGCTGGTTTGGC
AGGTTTATTTTCTGGAATTTTAAACGTTAGATTAATGCATTCAGGAGGATATGGTATTTTCTCAATAGTAGGTTATATCG
CTGGTGGTCCAATGAATGTAGCAATGTTTGTTGTTTCTTTATTAATAGCTGCCTCATCTGCTGCAATTATTTGTTTGTTT
ATTTATCAAGAAAGACCTTCAGAATTAAAATCAATTAAAAAAATTAGCAGAGTAATTATTAAAAATTATGCATTAAAAAC
TGGTTTAAAAAATAATGAAGTTGAAAAATTGCTTGATGAAAAATTATCTTTAATACAAAATTTTGTTAAAAAAGATGATA
TAGAAAAAATCAAAAAAGTTGAAGATAGTTATATTAAAATTTCAAAACTAGAAGCAACAATAGATTTATTAAATTCTAAA
GATGAAAAAGCTCAACAAAAATTGAATGAAAAAATGATTAGAGCAAATGAAAAAAATAACTTATTAAATGCTAAAAAAAT
TTATGACGAATTAGTAAACTTCAAACAAAATTCTAAAATTGAGAAATTAATAAATGAATTAAATGAAGTAAAAAAAGCCA
ATGTTAATTTGGAAAACTGATTAAAAGATCATCAAGATAAATTTATGGAGTTAACTAATGAAATTTTACTTAAAATATCA
AAAGAAATAGAACTTAATGATATTAATGATTTAAATAGCAATTATTTTAATGCAATACATTCAATTGATATAAATTACTT
AATAACTGAAAAACAAGAAACATTTTTTAATCAAAAAAATGTTAAAAAAAATCTTTTAAATAATAGATAG

Upstream 100 bases:

>100_bases
GTAAAAACCACTTTAAAAAAATAAAAGTGGTTTTTTTGTTTGCTTTCTTTTAAGTTTTAAAAAGATTAAAAAATAATATT
TAAGTGAAAGGAAAAAAAGT

Downstream 100 bases:

>100_bases
AGAGAATAAATATGGAAATTAAAAAACTTAAAAAATTTGATGATGATTTTTTGTGAGGCGCTTCAATTTCTGCTTTTCAG
GCAGAAGGTGCTTCAACAAA

Product: beta-glucoside PTS system IIABC component

Products: NA

Alternate protein names: Phosphotransferase enzyme IIB component; PTS system EIIB component; Permease IIC component; PTS system EIIC component [H]

Number of amino acids: Translated: 849; Mature: 849

Protein sequence:

>849_residues
MKNKKIIIYAPVNGVIGKIKDLKDGVFSEGMLGEGVYIIPESKEFYSPIDKGELKLVTATKHAYYFEHENGPNILMHISL
DTEKHQGKPFKTLSNVGDKLTLATKIVSVDLQMCKKQDVNVATPILIDTKEFSEWKFKPLDLKGKVKRGQPIGEFVKVEE
KKINEESNDLTTFEFKNKYEKSAESIYEYIGTESNYSKVYNCMTRLRFYVKDKQIVNTEAIKKMPIVKGVNWNGEEIQII
IGGEVQKVKDAFENYVSNKNISKTSKKQEGFEQKIKPKKPMKEAILGAISGIMVPTLPVFITVGMLMGLKAILVQAGAMP
EIVTGIAKEGQVSLTDADVFSAIFFIITEIAFKFLGIFIGYNTMKYLGGNTIMSILIGLALANPYIWQGTSWPLFTIGST
TIKVQAFTSSIMPHIAANIIFFYLDKWIKTWMPSVVDIIFRPMIAFLTTMLLAFFIVGPSLFLVEQGLAVIVGWLGSIPF
GIGVAIYAILWQPFVISGMHIAIAMPLSIGLMQGTPQVISPAIMYGCYGQLGAMIGVMIMTKNSQLKATCIANLTPGVIA
ITEPIIYGITLPKGRPFIAGCLGAGLAGLFSGILNVRLMHSGGYGIFSIVGYIAGGPMNVAMFVVSLLIAASSAAIICLF
IYQERPSELKSIKKISRVIIKNYALKTGLKNNEVEKLLDEKLSLIQNFVKKDDIEKIKKVEDSYIKISKLEATIDLLNSK
DEKAQQKLNEKMIRANEKNNLLNAKKIYDELVNFKQNSKIEKLINELNEVKKANVNLENWLKDHQDKFMELTNEILLKIS
KEIELNDINDLNSNYFNAIHSIDINYLITEKQETFFNQKNVKKNLLNNR

Sequences:

>Translated_849_residues
MKNKKIIIYAPVNGVIGKIKDLKDGVFSEGMLGEGVYIIPESKEFYSPIDKGELKLVTATKHAYYFEHENGPNILMHISL
DTEKHQGKPFKTLSNVGDKLTLATKIVSVDLQMCKKQDVNVATPILIDTKEFSE*KFKPLDLKGKVKRGQPIGEFVKVEE
KKINEESNDLTTFEFKNKYEKSAESIYEYIGTESNYSKVYNCMTRLRFYVKDKQIVNTEAIKKMPIVKGVN*NGEEIQII
IGGEVQKVKDAFENYVSNKNISKTSKKQEGFEQKIKPKKPMKEAILGAISGIMVPTLPVFITVGMLMGLKAILVQAGAMP
EIVTGIAKEGQVSLTDADVFSAIFFIITEIAFKFLGIFIGYNTMKYLGGNTIMSILIGLALANPYI*QGTS*PLFTIGST
TIKVQAFTSSIMPHIAANIIFFYLDK*IKT*MPSVVDIIFRPMIAFLTTMLLAFFIVGPSLFLVEQGLAVIVG*LGSIPF
GIGVAIYAILWQPFVISGMHIAIAMPLSIGLMQGTPQVISPAIMYGCYGQLGAMIGVMIMTKNSQLKATCIANLTPGVIA
ITEPIIYGITLPKGRPFIAGCLGAGLAGLFSGILNVRLMHSGGYGIFSIVGYIAGGPMNVAMFVVSLLIAASSAAIICLF
IYQERPSELKSIKKISRVIIKNYALKTGLKNNEVEKLLDEKLSLIQNFVKKDDIEKIKKVEDSYIKISKLEATIDLLNSK
DEKAQQKLNEKMIRANEKNNLLNAKKIYDELVNFKQNSKIEKLINELNEVKKANVNLEN*LKDHQDKFMELTNEILLKIS
KEIELNDINDLNSNYFNAIHSIDINYLITEKQETFFNQKNVKKNLLNNR
>Mature_849_residues
MKNKKIIIYAPVNGVIGKIKDLKDGVFSEGMLGEGVYIIPESKEFYSPIDKGELKLVTATKHAYYFEHENGPNILMHISL
DTEKHQGKPFKTLSNVGDKLTLATKIVSVDLQMCKKQDVNVATPILIDTKEFSE*KFKPLDLKGKVKRGQPIGEFVKVEE
KKINEESNDLTTFEFKNKYEKSAESIYEYIGTESNYSKVYNCMTRLRFYVKDKQIVNTEAIKKMPIVKGVN*NGEEIQII
IGGEVQKVKDAFENYVSNKNISKTSKKQEGFEQKIKPKKPMKEAILGAISGIMVPTLPVFITVGMLMGLKAILVQAGAMP
EIVTGIAKEGQVSLTDADVFSAIFFIITEIAFKFLGIFIGYNTMKYLGGNTIMSILIGLALANPYI*QGTS*PLFTIGST
TIKVQAFTSSIMPHIAANIIFFYLDK*IKT*MPSVVDIIFRPMIAFLTTMLLAFFIVGPSLFLVEQGLAVIVG*LGSIPF
GIGVAIYAILWQPFVISGMHIAIAMPLSIGLMQGTPQVISPAIMYGCYGQLGAMIGVMIMTKNSQLKATCIANLTPGVIA
ITEPIIYGITLPKGRPFIAGCLGAGLAGLFSGILNVRLMHSGGYGIFSIVGYIAGGPMNVAMFVVSLLIAASSAAIICLF
IYQERPSELKSIKKISRVIIKNYALKTGLKNNEVEKLLDEKLSLIQNFVKKDDIEKIKKVEDSYIKISKLEATIDLLNSK
DEKAQQKLNEKMIRANEKNNLLNAKKIYDELVNFKQNSKIEKLINELNEVKKANVNLEN*LKDHQDKFMELTNEILLKIS
KEIELNDINDLNSNYFNAIHSIDINYLITEKQETFFNQKNVKKNLLNNR

Specific function: The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane [H]

COG id: COG1263

COG function: function code G; Phosphotransferase system IIC components, glucose/maltose/N-acetylglucosamine-specific

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIC type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI2367362, Length=481, Percent_Identity=24.1164241164241, Blast_Score=130, Evalue=5e-31,
Organism=Escherichia coli, GI1788769, Length=419, Percent_Identity=27.6849642004773, Blast_Score=118, Evalue=2e-27,
Organism=Escherichia coli, GI1790159, Length=468, Percent_Identity=26.4957264957265, Blast_Score=117, Evalue=2e-27,
Organism=Escherichia coli, GI48994906, Length=489, Percent_Identity=25.7668711656442, Blast_Score=100, Evalue=3e-22,
Organism=Escherichia coli, GI1788757, Length=143, Percent_Identity=29.3706293706294, Blast_Score=73, Evalue=9e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018113
- InterPro:   IPR001996
- InterPro:   IPR003352
- InterPro:   IPR013013 [H]

Pfam domain/function: PF00367 PTS_EIIB; PF02378 PTS_EIIC [H]

EC number: =2.7.1.69 [H]

Molecular weight: Translated: 93297; Mature: 93297

Theoretical pI: Translated: 9.58; Mature: 9.58

Prosite motif: PS51093 PTS_EIIA_TYPE_1 ; PS01035 PTS_EIIB_TYPE_1_CYS ; PS51098 PTS_EIIB_TYPE_1 ; PS51103 PTS_EIIC_TYPE_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNKKIIIYAPVNGVIGKIKDLKDGVFSEGMLGEGVYIIPESKEFYSPIDKGELKLVTAT
CCCCEEEEEECCCHHHHHHHHHHCCHHCCCCCCCCEEEEECCHHHHCCCCCCCEEEEEEE
KHAYYFEHENGPNILMHISLDTEKHQGKPFKTLSNVGDKLTLATKIVSVDLQMCKKQDVN
EEEEEEEECCCCCEEEEEEECCCCCCCCCHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCC
VATPILIDTKEFSEKFKPLDLKGKVKRGQPIGEFVKVEEKKINEESNDLTTFEFKNKYEK
CCCCEEEECHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCEEEHHHHHHHH
SAESIYEYIGTESNYSKVYNCMTRLRFYVKDKQIVNTEAIKKMPIVKGVNNGEEIQIIIG
HHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCHHHCHHHHHHCCCCCCCCCCCEEEEEEC
GEVQKVKDAFENYVSNKNISKTSKKQEGFEQKIKPKKPMKEAILGAISGIMVPTLPVFIT
CCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHH
VGMLMGLKAILVQAGAMPEIVTGIAKEGQVSLTDADVFSAIFFIITEIAFKFLGIFIGYN
HHHHHHHHHHHHHCCCCHHHHHHHCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHH
TMKYLGGNTIMSILIGLALANPYIQGTSPLFTIGSTTIKVQAFTSSIMPHIAANIIFFYL
HHHHHCCHHHHHHHHHHHHCCCCCCCCCCEEEECCCEEEEEHHHHHHHHHHHHHHHHHHH
DKIKTMPSVVDIIFRPMIAFLTTMLLAFFIVGPSLFLVEQGLAVIVGLGSIPFGIGVAIY
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCEEEEECCCCCHHHHHHHH
AILWQPFVISGMHIAIAMPLSIGLMQGTPQVISPAIMYGCYGQLGAMIGVMIMTKNSQLK
HHHHHHHHHCCCCEEEEEHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHEEEEEECCCCEE
ATCIANLTPGVIAITEPIIYGITLPKGRPFIAGCLGAGLAGLFSGILNVRLMHSGGYGIF
EEEEECCCCCEEEEECCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHEEEEECCCCHHH
SIVGYIAGGPMNVAMFVVSLLIAASSAAIICLFIYQERPSELKSIKKISRVIIKNYALKT
HHHHHHCCCCHHHHHHHHHHHHHCCCCEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHC
GLKNNEVEKLLDEKLSLIQNFVKKDDIEKIKKVEDSYIKISKLEATIDLLNSKDEKAQQK
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEHHHHHHHHHHHCCCCHHHHHH
LNEKMIRANEKNNLLNAKKIYDELVNFKQNSKIEKLINELNEVKKANVNLENLKDHQDKF
HHHHHHHCCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
MELTNEILLKISKEIELNDINDLNSNYFNAIHSIDINYLITEKQETFFNQKNVKKNLLNN
HHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHCCEEEEEECHHHHHHHHHHHHHHHCCC
R
C
>Mature Secondary Structure
MKNKKIIIYAPVNGVIGKIKDLKDGVFSEGMLGEGVYIIPESKEFYSPIDKGELKLVTAT
CCCCEEEEEECCCHHHHHHHHHHCCHHCCCCCCCCEEEEECCHHHHCCCCCCCEEEEEEE
KHAYYFEHENGPNILMHISLDTEKHQGKPFKTLSNVGDKLTLATKIVSVDLQMCKKQDVN
EEEEEEEECCCCCEEEEEEECCCCCCCCCHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCC
VATPILIDTKEFSEKFKPLDLKGKVKRGQPIGEFVKVEEKKINEESNDLTTFEFKNKYEK
CCCCEEEECHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCEEEHHHHHHHH
SAESIYEYIGTESNYSKVYNCMTRLRFYVKDKQIVNTEAIKKMPIVKGVNNGEEIQIIIG
HHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCHHHCHHHHHHCCCCCCCCCCCEEEEEEC
GEVQKVKDAFENYVSNKNISKTSKKQEGFEQKIKPKKPMKEAILGAISGIMVPTLPVFIT
CCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHH
VGMLMGLKAILVQAGAMPEIVTGIAKEGQVSLTDADVFSAIFFIITEIAFKFLGIFIGYN
HHHHHHHHHHHHHCCCCHHHHHHHCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHH
TMKYLGGNTIMSILIGLALANPYIQGTSPLFTIGSTTIKVQAFTSSIMPHIAANIIFFYL
HHHHHCCHHHHHHHHHHHHCCCCCCCCCCEEEECCCEEEEEHHHHHHHHHHHHHHHHHHH
DKIKTMPSVVDIIFRPMIAFLTTMLLAFFIVGPSLFLVEQGLAVIVGLGSIPFGIGVAIY
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCEEEEECCCCCHHHHHHHH
AILWQPFVISGMHIAIAMPLSIGLMQGTPQVISPAIMYGCYGQLGAMIGVMIMTKNSQLK
HHHHHHHHHCCCCEEEEEHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHEEEEEECCCCEE
ATCIANLTPGVIAITEPIIYGITLPKGRPFIAGCLGAGLAGLFSGILNVRLMHSGGYGIF
EEEEECCCCCEEEEECCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHEEEEECCCCHHH
SIVGYIAGGPMNVAMFVVSLLIAASSAAIICLFIYQERPSELKSIKKISRVIIKNYALKT
HHHHHHCCCCHHHHHHHHHHHHHCCCCEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHC
GLKNNEVEKLLDEKLSLIQNFVKKDDIEKIKKVEDSYIKISKLEATIDLLNSKDEKAQQK
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEHHHHHHHHHHHCCCCHHHHHH
LNEKMIRANEKNNLLNAKKIYDELVNFKQNSKIEKLINELNEVKKANVNLENLKDHQDKF
HHHHHHHCCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
MELTNEILLKISKEIELNDINDLNSNYFNAIHSIDINYLITEKQETFFNQKNVKKNLLNN
HHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHCCEEEEEECHHHHHHHHHHHHHHHCCC
R
C

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]