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The map label for this gene is infB

Identifier: 50365111

GI number: 50365111

Start: 338874

End: 340763

Strand: Direct

Name: infB

Synonym: Mfl295

Alternate gene names: 50365111

Gene position: 338874-340763 (Clockwise)

Preceding gene: 50365110

Following gene: 50365112

Centisome position: 42.72

GC content: 31.01

Gene sequence:

>1890_bases
ATGGCTAAAAATATAAAAACAAATAAAAAACCACAACAAGTAAACAAAAAAGAAATGTCTAAACAACATGCTAAACAAAT
TAAACAACAATTAAATGAAACAGTTGCAACTGGAATCATTGATGGGGTGTTTGTTTATACTGAAGCTTTAAGCATTGCTG
ATTTTGCTAACCAAATTGGGAAAAGTGTTGCTGAAATACTTAAATATTTCTTTGCACAAGGACTAATGTTAAATCAAAAT
GTTGTGTTATCAGAAGAACAAATGGCTGAACTTGCTTTAGAGTTTGGTTTTGACTTTAGAAAAGAAGAATCTTTAACTAA
AGAAAACTTCTTTGAAGCATTAGATGCTAGTGAAGAAGATAAACCAGAAGATTTAGAACATAGAGCACCAATTGTAACAA
TTATGGGTCACGTTGACCATGGTAAAACTACTTTATTAGACTCTATCAAAAACACTAATGTTGTTGGTGGAGAAGCTGGG
GGAATTACTCAAGCAATTGGAGCTTACCAAGTTAAAAACAAAGATGGTAAAAAAATAACATTTATTGATACTCCAGGTCA
CGAGGCATTTTCAGAAATGCGTAGTCGTGGGGCAAATGTAACTGATATTGTTATTTTAATTGTTGCTGCTGATGATGGGG
TTATGCCTCAAACAGAAGAAGCAATTGATCATGCTAAATTAGCAAATGTACCAATTATTGTATTTATTAATAAATGTGAT
AAACCAGGAGCAGATCCTGAACGTGTTAAAGCTGAATTAATGAAATATGAAATAGTAGCTGAAGAATATGGTGGAGATAT
TCCATTTGTTCAAGGTAGTGCTAAACAAAAGATTGGTTTAGACCAATTAGAAGAAACTATTTTATTAATTGCTGAAATGC
AAGATTATAAAGCAAATCCAAATAAATTAGCTAAAGGGGTTGTTATTGAAGCTCACTTAGATAAAGCAAAAGGACCAGTT
GCTTCTATTCTTGTTAAAGAAGGTACTTTAGATATTAGAGATATGATTATCGCTGGAACTACATATGGAAATATTAAACA
TATGGAAGACGAAACTAATAAAAAAGTATTAAAAGCAGGACCAAGTAAACCAGTTGTTGTTTATGGATTAAATGAAGTTC
CAAGTGCTGGAGATAAATTCATTGTTATGAATGATGAAAAAATGGCTAGAACTATTGCTGAAGCACAAGCTGAAAAGAAA
TTAGCAGCTGAACGTCAATCAAACCAAATCTTTAGTTTAGATTCAATTAAAAAACATATTGATGATGGTGAGTTAAAAGC
AATTAACTTAATTGTTAAAGCTGATACTCAAGGTTCAGTTGAAGCACTTAAAGGAAGTTTAACTAAAATTGACATACCTG
GTGTTAAATTAAATATTATTAGAGCATCTGTTGGAACTATTACATTAAGTGATGTTACTTTAGCTTCAACTGTAACTGAT
GGAATTGTATTAATCTATGGATTTAATGTAAGACCAGATGCAGTTGTACGTAAAAAAGCTGAAGAAGAAGGTATTGAAAT
TCGTTTACATAATATTATTTATAAAGTAATTGAAGAATTAGAAGATGCAGCTAAAGGGATGTTAGATCCAGAATACAAAG
AAGTAGTTACTGGATCAGCTGAAATTAGAGCAACATTCAAACACTCTGACATCGGAACAATTGGTGGGTTCCATATTACT
GATGGAAGCATTGAACGTAAATCTAAAGTTCGCATTATTAGAAATGGTATTGTTATTTATACTGGAGAATTAGCAACTTT
AAAACATTTAAAAGATGATATTAAAGAAGCTAAAATCAACTCAGAAGGTGGATTAACAATCAAAAACTTTAATGATATCA
AAGAAGGCGATATTGTTGAAGGATATAAGGAAGAAGAAGTTAAGAAATAG

Upstream 100 bases:

>100_bases
ACAAGCGTGTGGATCTACCACAATTGTAGCGATTGGTGTTAATGATATTAATATTATTAAATTAATTAAAAACAATTTAT
AGAAGAAAGGGGATTTTTAC

Downstream 100 bases:

>100_bases
GGGAATTTAGCATATGAAAAAAATTGAAATAAATAATTTTAGATCAATTGAAAAAGCAATAATGAATTTTGGTGAAATAA
ATTTTTTTATAGGAGATAAT

Product: translation initiation factor IF-2

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 629; Mature: 628

Protein sequence:

>629_residues
MAKNIKTNKKPQQVNKKEMSKQHAKQIKQQLNETVATGIIDGVFVYTEALSIADFANQIGKSVAEILKYFFAQGLMLNQN
VVLSEEQMAELALEFGFDFRKEESLTKENFFEALDASEEDKPEDLEHRAPIVTIMGHVDHGKTTLLDSIKNTNVVGGEAG
GITQAIGAYQVKNKDGKKITFIDTPGHEAFSEMRSRGANVTDIVILIVAADDGVMPQTEEAIDHAKLANVPIIVFINKCD
KPGADPERVKAELMKYEIVAEEYGGDIPFVQGSAKQKIGLDQLEETILLIAEMQDYKANPNKLAKGVVIEAHLDKAKGPV
ASILVKEGTLDIRDMIIAGTTYGNIKHMEDETNKKVLKAGPSKPVVVYGLNEVPSAGDKFIVMNDEKMARTIAEAQAEKK
LAAERQSNQIFSLDSIKKHIDDGELKAINLIVKADTQGSVEALKGSLTKIDIPGVKLNIIRASVGTITLSDVTLASTVTD
GIVLIYGFNVRPDAVVRKKAEEEGIEIRLHNIIYKVIEELEDAAKGMLDPEYKEVVTGSAEIRATFKHSDIGTIGGFHIT
DGSIERKSKVRIIRNGIVIYTGELATLKHLKDDIKEAKINSEGGLTIKNFNDIKEGDIVEGYKEEEVKK

Sequences:

>Translated_629_residues
MAKNIKTNKKPQQVNKKEMSKQHAKQIKQQLNETVATGIIDGVFVYTEALSIADFANQIGKSVAEILKYFFAQGLMLNQN
VVLSEEQMAELALEFGFDFRKEESLTKENFFEALDASEEDKPEDLEHRAPIVTIMGHVDHGKTTLLDSIKNTNVVGGEAG
GITQAIGAYQVKNKDGKKITFIDTPGHEAFSEMRSRGANVTDIVILIVAADDGVMPQTEEAIDHAKLANVPIIVFINKCD
KPGADPERVKAELMKYEIVAEEYGGDIPFVQGSAKQKIGLDQLEETILLIAEMQDYKANPNKLAKGVVIEAHLDKAKGPV
ASILVKEGTLDIRDMIIAGTTYGNIKHMEDETNKKVLKAGPSKPVVVYGLNEVPSAGDKFIVMNDEKMARTIAEAQAEKK
LAAERQSNQIFSLDSIKKHIDDGELKAINLIVKADTQGSVEALKGSLTKIDIPGVKLNIIRASVGTITLSDVTLASTVTD
GIVLIYGFNVRPDAVVRKKAEEEGIEIRLHNIIYKVIEELEDAAKGMLDPEYKEVVTGSAEIRATFKHSDIGTIGGFHIT
DGSIERKSKVRIIRNGIVIYTGELATLKHLKDDIKEAKINSEGGLTIKNFNDIKEGDIVEGYKEEEVKK
>Mature_628_residues
AKNIKTNKKPQQVNKKEMSKQHAKQIKQQLNETVATGIIDGVFVYTEALSIADFANQIGKSVAEILKYFFAQGLMLNQNV
VLSEEQMAELALEFGFDFRKEESLTKENFFEALDASEEDKPEDLEHRAPIVTIMGHVDHGKTTLLDSIKNTNVVGGEAGG
ITQAIGAYQVKNKDGKKITFIDTPGHEAFSEMRSRGANVTDIVILIVAADDGVMPQTEEAIDHAKLANVPIIVFINKCDK
PGADPERVKAELMKYEIVAEEYGGDIPFVQGSAKQKIGLDQLEETILLIAEMQDYKANPNKLAKGVVIEAHLDKAKGPVA
SILVKEGTLDIRDMIIAGTTYGNIKHMEDETNKKVLKAGPSKPVVVYGLNEVPSAGDKFIVMNDEKMARTIAEAQAEKKL
AAERQSNQIFSLDSIKKHIDDGELKAINLIVKADTQGSVEALKGSLTKIDIPGVKLNIIRASVGTITLSDVTLASTVTDG
IVLIYGFNVRPDAVVRKKAEEEGIEIRLHNIIYKVIEELEDAAKGMLDPEYKEVVTGSAEIRATFKHSDIGTIGGFHITD
GSIERKSKVRIIRNGIVIYTGELATLKHLKDDIKEAKINSEGGLTIKNFNDIKEGDIVEGYKEEEVKK

Specific function: One of the essential components for the initiation of protein synthesis. Protects formylmethionyl-tRNA from spontaneous hydrolysis and promotes its binding to the 30S ribosomal subunits. Also involved in the hydrolysis of GTP during the formation of the 7

COG id: COG0532

COG function: function code J; Translation initiation factor 2 (IF-2; GTPase)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the IF-2 family

Homologues:

Organism=Homo sapiens, GI53729339, Length=549, Percent_Identity=39.8907103825137, Blast_Score=373, Evalue=1e-103,
Organism=Homo sapiens, GI53729337, Length=549, Percent_Identity=39.8907103825137, Blast_Score=373, Evalue=1e-103,
Organism=Homo sapiens, GI84043963, Length=131, Percent_Identity=45.8015267175573, Blast_Score=114, Evalue=2e-25,
Organism=Homo sapiens, GI157426893, Length=229, Percent_Identity=29.6943231441048, Blast_Score=79, Evalue=1e-14,
Organism=Escherichia coli, GI1789559, Length=589, Percent_Identity=45.3310696095076, Blast_Score=489, Evalue=1e-139,
Organism=Escherichia coli, GI2367247, Length=179, Percent_Identity=34.0782122905028, Blast_Score=83, Evalue=4e-17,
Organism=Escherichia coli, GI1790412, Length=206, Percent_Identity=34.4660194174757, Blast_Score=77, Evalue=3e-15,
Organism=Escherichia coli, GI1789737, Length=206, Percent_Identity=34.4660194174757, Blast_Score=77, Evalue=4e-15,
Organism=Escherichia coli, GI1788922, Length=234, Percent_Identity=29.9145299145299, Blast_Score=75, Evalue=1e-14,
Organism=Escherichia coli, GI48994988, Length=171, Percent_Identity=34.5029239766082, Blast_Score=73, Evalue=7e-14,
Organism=Caenorhabditis elegans, GI71994658, Length=551, Percent_Identity=36.6606170598911, Blast_Score=320, Evalue=2e-87,
Organism=Caenorhabditis elegans, GI212656558, Length=131, Percent_Identity=41.9847328244275, Blast_Score=108, Evalue=1e-23,
Organism=Caenorhabditis elegans, GI17557151, Length=320, Percent_Identity=29.375, Blast_Score=92, Evalue=9e-19,
Organism=Caenorhabditis elegans, GI17556456, Length=165, Percent_Identity=34.5454545454545, Blast_Score=69, Evalue=1e-11,
Organism=Saccharomyces cerevisiae, GI6324550, Length=654, Percent_Identity=33.7920489296636, Blast_Score=340, Evalue=3e-94,
Organism=Saccharomyces cerevisiae, GI6319282, Length=131, Percent_Identity=44.2748091603053, Blast_Score=111, Evalue=3e-25,
Organism=Saccharomyces cerevisiae, GI6324761, Length=161, Percent_Identity=36.0248447204969, Blast_Score=71, Evalue=6e-13,
Organism=Saccharomyces cerevisiae, GI6323098, Length=146, Percent_Identity=32.1917808219178, Blast_Score=66, Evalue=1e-11,
Organism=Drosophila melanogaster, GI28572034, Length=534, Percent_Identity=36.7041198501873, Blast_Score=322, Evalue=4e-88,
Organism=Drosophila melanogaster, GI160714833, Length=76, Percent_Identity=64.4736842105263, Blast_Score=111, Evalue=1e-24,
Organism=Drosophila melanogaster, GI24656849, Length=143, Percent_Identity=42.6573426573427, Blast_Score=104, Evalue=2e-22,
Organism=Drosophila melanogaster, GI281363316, Length=163, Percent_Identity=36.8098159509202, Blast_Score=67, Evalue=5e-11,
Organism=Drosophila melanogaster, GI17864358, Length=163, Percent_Identity=36.8098159509202, Blast_Score=67, Evalue=5e-11,
Organism=Drosophila melanogaster, GI78706572, Length=218, Percent_Identity=27.0642201834862, Blast_Score=67, Evalue=5e-11,
Organism=Drosophila melanogaster, GI19922690, Length=176, Percent_Identity=29.5454545454545, Blast_Score=65, Evalue=1e-10,

Paralogues:

None

Copy number: 1150 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): IF2_MESFL (Q6F1H1)

Other databases:

- EMBL:   AE017263
- RefSeq:   YP_053536.1
- ProteinModelPortal:   Q6F1H1
- GeneID:   2898273
- GenomeReviews:   AE017263_GR
- KEGG:   mfl:Mfl295
- HOGENOM:   HBG635083
- OMA:   QYGRIRS
- ProtClustDB:   PRK05306
- BioCyc:   MFLO265311:MFL295-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00100_B
- InterPro:   IPR006847
- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR000178
- InterPro:   IPR015760
- InterPro:   IPR023115
- InterPro:   IPR009000
- Gene3D:   G3DSA:3.40.50.10050
- PANTHER:   PTHR23115:SF41
- TIGRFAMs:   TIGR00487
- TIGRFAMs:   TIGR00231

Pfam domain/function: PF00009 GTP_EFTU; PF11987 IF-2; PF04760 IF2_N; SSF52156 TIF_IF2_dom3; SSF50447 Translat_factor

EC number: NA

Molecular weight: Translated: 68991; Mature: 68860

Theoretical pI: Translated: 5.25; Mature: 5.25

Prosite motif: PS01176 IF2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKNIKTNKKPQQVNKKEMSKQHAKQIKQQLNETVATGIIDGVFVYTEALSIADFANQIG
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KSVAEILKYFFAQGLMLNQNVVLSEEQMAELALEFGFDFRKEESLTKENFFEALDASEED
HHHHHHHHHHHHCCEEECCCEEECHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCC
KPEDLEHRAPIVTIMGHVDHGKTTLLDSIKNTNVVGGEAGGITQAIGAYQVKNKDGKKIT
CCCCHHHCCCEEEEEECCCCCHHHHHHHHCCCCCCCCCCCCHHHHHCCEEEECCCCCEEE
FIDTPGHEAFSEMRSRGANVTDIVILIVAADDGVMPQTEEAIDHAKLANVPIIVFINKCD
EEECCCHHHHHHHHHCCCCCEEEEEEEEECCCCCCCCHHHHHHHHHHCCCCEEEEEECCC
KPGADPERVKAELMKYEIVAEEYGGDIPFVQGSAKQKIGLDQLEETILLIAEMQDYKANP
CCCCCHHHHHHHHHHHHHHHHHHCCCCCEECCCCHHCCCHHHHHHHHHHEEHHHHCCCCH
NKLAKGVVIEAHLDKAKGPVASILVKEGTLDIRDMIIAGTTYGNIKHMEDETNKKVLKAG
HHHHCCEEEEEECCCCCCCCEEEEEECCCCCEEHEEEECCCCCCCEECCCHHCCHHHHCC
PSKPVVVYGLNEVPSAGDKFIVMNDEKMARTIAEAQAEKKLAAERQSNQIFSLDSIKKHI
CCCCEEEEECCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHCCCEEEHHHHHHHC
DDGELKAINLIVKADTQGSVEALKGSLTKIDIPGVKLNIIRASVGTITLSDVTLASTVTD
CCCCEEEEEEEEEECCCCCHHHHCCCCEEEECCCEEEEEEEEECCEEEECCEEEHHHHCC
GIVLIYGFNVRPDAVVRKKAEEEGIEIRLHNIIYKVIEELEDAAKGMLDPEYKEVVTGSA
CEEEEEECCCCCHHHHHHHHHHCCCEEEHHHHHHHHHHHHHHHHCCCCCCCHHHHHCCCH
EIRATFKHSDIGTIGGFHITDGSIERKSKVRIIRNGIVIYTGELATLKHLKDDIKEAKIN
HEEEEEECCCCCCCCCEEECCCCCCCHHHEEEEECCEEEEECCHHHHHHHHHHHHHHCCC
SEGGLTIKNFNDIKEGDIVEGYKEEEVKK
CCCCEEECCCCCCCCCCCCCCCCHHHCCC
>Mature Secondary Structure 
AKNIKTNKKPQQVNKKEMSKQHAKQIKQQLNETVATGIIDGVFVYTEALSIADFANQIG
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KSVAEILKYFFAQGLMLNQNVVLSEEQMAELALEFGFDFRKEESLTKENFFEALDASEED
HHHHHHHHHHHHCCEEECCCEEECHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCC
KPEDLEHRAPIVTIMGHVDHGKTTLLDSIKNTNVVGGEAGGITQAIGAYQVKNKDGKKIT
CCCCHHHCCCEEEEEECCCCCHHHHHHHHCCCCCCCCCCCCHHHHHCCEEEECCCCCEEE
FIDTPGHEAFSEMRSRGANVTDIVILIVAADDGVMPQTEEAIDHAKLANVPIIVFINKCD
EEECCCHHHHHHHHHCCCCCEEEEEEEEECCCCCCCCHHHHHHHHHHCCCCEEEEEECCC
KPGADPERVKAELMKYEIVAEEYGGDIPFVQGSAKQKIGLDQLEETILLIAEMQDYKANP
CCCCCHHHHHHHHHHHHHHHHHHCCCCCEECCCCHHCCCHHHHHHHHHHEEHHHHCCCCH
NKLAKGVVIEAHLDKAKGPVASILVKEGTLDIRDMIIAGTTYGNIKHMEDETNKKVLKAG
HHHHCCEEEEEECCCCCCCCEEEEEECCCCCEEHEEEECCCCCCCEECCCHHCCHHHHCC
PSKPVVVYGLNEVPSAGDKFIVMNDEKMARTIAEAQAEKKLAAERQSNQIFSLDSIKKHI
CCCCEEEEECCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHCCCEEEHHHHHHHC
DDGELKAINLIVKADTQGSVEALKGSLTKIDIPGVKLNIIRASVGTITLSDVTLASTVTD
CCCCEEEEEEEEEECCCCCHHHHCCCCEEEECCCEEEEEEEEECCEEEECCEEEHHHHCC
GIVLIYGFNVRPDAVVRKKAEEEGIEIRLHNIIYKVIEELEDAAKGMLDPEYKEVVTGSA
CEEEEEECCCCCHHHHHHHHHHCCCEEEHHHHHHHHHHHHHHHHCCCCCCCHHHHHCCCH
EIRATFKHSDIGTIGGFHITDGSIERKSKVRIIRNGIVIYTGELATLKHLKDDIKEAKIN
HEEEEEECCCCCCCCCEEECCCCCCCHHHEEEEECCEEEEECCHHHHHHHHHHHHHHCCC
SEGGLTIKNFNDIKEGDIVEGYKEEEVKK
CCCCEEECCCCCCCCCCCCCCCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; In phosphorus-containing anhydrides [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA