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The map label for this gene is trmB [H]

Identifier: 50365034

GI number: 50365034

Start: 244281

End: 244952

Strand: Direct

Name: trmB [H]

Synonym: Mfl218

Alternate gene names: 50365034

Gene position: 244281-244952 (Clockwise)

Preceding gene: 50365033

Following gene: 50365035

Centisome position: 30.8

GC content: 24.11

Gene sequence:

>672_bases
ATGAGATTAAGAAATAAACCTTGAGTAAAGGAATATTTAGAAAAAAATGATAAATATTTAATTAGTTGAGATAAAGAAAC
AAAAATTAATTTATCTGATTTATTCAATAATAAAAAGCAACCAGTTCACTTAGAAATTGGCTGTGGTAAAGGAAATTTTA
TTACAAACCATGCATTAAAAGAATCAGATATTAATTTTATTGGAATGGAAAAAGAAGAAACTGTTGTTGGAGTAGCATTG
AAAAAAACTTTAGCAGAATTTGAACAAAGAAATAAAGAAGTAACTAATTTAAAGTACTTTAATGATTTTGCTGAAGACTT
ATCAGATATTTTTGCCCCATCATCAATTGATAAAATTTATTTAAACTTTTCTGATCCTTGACCAAAGGCTCGACATTCTA
AAAAAAGACTTACATATAGAACATTTTTAGACATATATGCCAACATAATAAAATCTCATGGAATACTTGAGTTTAAAACA
GATAATGATGGATTATTTGCTTTTTCATTAGAAGAGATAGCTGAAAATAAAAACTGAGAACTTATATATCAAACAACTGA
TTTATATTCAGATATTGAGGCTTTAAAAAATAATATACCTACTGAATATGAGACAAAATTTCATACAGCTGGTAAAAATA
TAAATAAATTAATTATTAAAAAAACTTTTTAG

Upstream 100 bases:

>100_bases
ATCCAACAATTGCATCTTCACCATTAATAACTAGTTTAGTTGATATAATGTGTGTACTTGTATTTATTGGGTTTGCATTA
ATTATAGTTTAGGAGAAAAT

Downstream 100 bases:

>100_bases
AAACTTGGAGAAATCCAAGTTTTTTTATTAATAATATCAAGAGGTGGTTTATCGTGAATCTAATTCTTACATTTTTATTT
TTTATATTTAGTTTTAATCA

Product: S-adenosylmethionine-dependent methyltransferase

Products: NA

Alternate protein names: tRNA(m7G46)-methyltransferase [H]

Number of amino acids: Translated: 223; Mature: 223

Protein sequence:

>223_residues
MRLRNKPWVKEYLEKNDKYLISWDKETKINLSDLFNNKKQPVHLEIGCGKGNFITNHALKESDINFIGMEKEETVVGVAL
KKTLAEFEQRNKEVTNLKYFNDFAEDLSDIFAPSSIDKIYLNFSDPWPKARHSKKRLTYRTFLDIYANIIKSHGILEFKT
DNDGLFAFSLEEIAENKNWELIYQTTDLYSDIEALKNNIPTEYETKFHTAGKNINKLIIKKTF

Sequences:

>Translated_223_residues
MRLRNKP*VKEYLEKNDKYLIS*DKETKINLSDLFNNKKQPVHLEIGCGKGNFITNHALKESDINFIGMEKEETVVGVAL
KKTLAEFEQRNKEVTNLKYFNDFAEDLSDIFAPSSIDKIYLNFSDP*PKARHSKKRLTYRTFLDIYANIIKSHGILEFKT
DNDGLFAFSLEEIAENKN*ELIYQTTDLYSDIEALKNNIPTEYETKFHTAGKNINKLIIKKTF
>Mature_223_residues
MRLRNKP*VKEYLEKNDKYLIS*DKETKINLSDLFNNKKQPVHLEIGCGKGNFITNHALKESDINFIGMEKEETVVGVAL
KKTLAEFEQRNKEVTNLKYFNDFAEDLSDIFAPSSIDKIYLNFSDP*PKARHSKKRLTYRTFLDIYANIIKSHGILEFKT
DNDGLFAFSLEEIAENKN*ELIYQTTDLYSDIEALKNNIPTEYETKFHTAGKNINKLIIKKTF

Specific function: Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA [H]

COG id: COG0220

COG function: function code R; Predicted S-adenosylmethionine-dependent methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. TrmB family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003358 [H]

Pfam domain/function: PF02390 Methyltransf_4 [H]

EC number: =2.1.1.33 [H]

Molecular weight: Translated: 25341; Mature: 25341

Theoretical pI: Translated: 7.70; Mature: 7.70

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
1.3 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLRNKPVKEYLEKNDKYLISDKETKINLSDLFNNKKQPVHLEIGCGKGNFITNHALKES
CCCCCCCHHHHHHCCCCEEEECCCCEEEHHHHHCCCCCCEEEEEECCCCCEEECCCCCCC
DINFIGMEKEETVVGVALKKTLAEFEQRNKEVTNLKYFNDFAEDLSDIFAPSSIDKIYLN
CCCEEECCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCCEEEEE
FSDPPKARHSKKRLTYRTFLDIYANIIKSHGILEFKTDNDGLFAFSLEEIAENKNELIYQ
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCEEEEEHHHHHCCCCCEEEE
TTDLYSDIEALKNNIPTEYETKFHTAGKNINKLIIKKTF
HHHHHHHHHHHHHCCCCCHHHHHHHCCCCCEEEEEEECC
>Mature Secondary Structure
MRLRNKPVKEYLEKNDKYLISDKETKINLSDLFNNKKQPVHLEIGCGKGNFITNHALKES
CCCCCCCHHHHHHCCCCEEEECCCCEEEHHHHHCCCCCCEEEEEECCCCCEEECCCCCCC
DINFIGMEKEETVVGVALKKTLAEFEQRNKEVTNLKYFNDFAEDLSDIFAPSSIDKIYLN
CCCEEECCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCCEEEEE
FSDPPKARHSKKRLTYRTFLDIYANIIKSHGILEFKTDNDGLFAFSLEEIAENKNELIYQ
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCEEEEEHHHHHCCCCCEEEE
TTDLYSDIEALKNNIPTEYETKFHTAGKNINKLIIKKTF
HHHHHHHHHHHHHCCCCCHHHHHHHCCCCCEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA