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The map label for this gene is glcB [H]

Identifier: 50365030

GI number: 50365030

Start: 237897

End: 240026

Strand: Direct

Name: glcB [H]

Synonym: Mfl214

Alternate gene names: 50365030

Gene position: 237897-240026 (Clockwise)

Preceding gene: 50365029

Following gene: 50365032

Centisome position: 29.99

GC content: 33.19

Gene sequence:

>2130_bases
ATGAAAAGTTTTTCTTCTATTGAATGAAAAAGTAGTGCTAAAAACTCTTGAGAAATGTTCAAAAAGGGTTGTGGAAACAT
TATGCCTACACTTTCAAAACTAAGTAAAGCTTTCCTTTTACCTATTGCTTTACTTCCAATCGCGGGAGTATTCTTAGGTG
TAGGGTCAGCTATAGCTTCAAATTCAGTTGATGGTACTTTTGGTTATTTCTTAGGATCATTGCTTAATAAAATGGGTGAT
GTATGTTTTGGGAACTTACCAGTACTGTTCTGTATTTCAGTTGCCCTTGCATATACTAAAGACTCAGGAATTGCAGCTAT
TACAGCTGTTGTAGGTTTCTTAGTTATGAATGGTATGCAAGCAGCGTTATTACATACTCAACAATTAAACATTGATACAA
CAAACTTATGAGTACAAACAAGTATCGATGAAAATGGTAAAGCTGTTTGAGAACATTTTACTAGTGTAGTTGTAGATGCA
AACGGAAAAGGTAAAATTAATTGAGCAGACTCTTCAATTAATTTACAAAATATAATGGCTTGAGGAATTGGTTCTAGTGA
AGATAATCTTCGTATGGTTAATTCAAATTTTGAATTAATTGATGGAGCTGGATCAGTTTACTATTCATTATTATGAATTA
ATAAAATTCCTAATGGTTTAATTACTTCAAATATTGGTGTAAATTCATTAAATACAGGAGTATTTGCAGGTATTTTCGTT
GGAGCTATCGCAGCTAAATGTTACAATAAATTTCATCAAACTCAATTACCATCAGCAATTAGTTTCTTTAGCGGAACAAA
ATTAGTTCCGATTGTAACTTTTGTTGCTGTTATACCATTATCATTTATTTTTATGTTTATTTGACCATATGTTGGTATGG
GATTAGCAGCATTTGGTCAAGCATCAGGAAAATTACCTGGTGGTTTAGATTCATTTATCTATGAAGTAGCAGAACGTTCA
TTAGTTCCATTTGGACTACACCACGTATTCTATGCTCCATTATGATGGACAAATGCTGGAGGTTCAATTGCTGAAGCATT
CAACAAAGCAACTCCAGAACAACAAGCAGAATTTGTTAAAATTTGATCAGCAAATCATTCAGTTTTAGCTGAAACACTTT
TAAACGGTGTTTCAGAAGGATTAAAATTCCAAACAATTAGAAATATGATTTCAAGTGATTATCCTGATTTATGAAAATCA
ATGGGTGATCAAACTATGGCATATTCAGTTATCGCAAAAATTAATGTATTAAACTTTACAGACTTAGAAGCTTTAGGGTT
AAACATAGGTAGATTCCAATCAGGTAAATTCGGATTTATGTTATTAGGATTACCAATGGCTGGATTAGCTATGTGATTAA
ACGTACCTAAAGAAAACAGAAAAGAAGTTATGGGAATTTACTTCTCAGCAGCCTTTACTTGTTTCTTAACAGGTATTACA
GAACCAATCGAATATACATTCTTATTCTTAGCACCATGATTATTCTATGGTGTACATATGCCTTTAGCAGCTATTTCATT
CTTATTTGCTGGGTTATTCAAAACTCACGTATCAATGACTGTTTCTGGAGGATTTATTGATTACATCGTATTCGGAGTTA
TCCCTTACTTTGGATCAAAAGCAATGTCAGCTAAATCAGCATTTGCAATCTTAGGAGTTTCAGCCGCAATGGCGCCAGCT
TACTTCTTCGGATTCTACTTTGCAGTAAAATACGGAAATGTTATGGTACCAGGTCGTGATGGAAGTACAAACGTGCAATT
AGCAACAAAAGCTGAATATAAAGCATCAAAAGGATTAAATGTTGATGGAAGCAAAATTGAAGAGGGAAAATCTACAAAAG
CATCTGCAAAAGATGCTGTTGAAGAAGCAAGAATTCAAAAAGCTACAAAAATCATCGAATTCTTAGGTGGAGAAGCTAAT
ATCGTTGACGTTGATGCATGTGCTAGCCGTTTACGTTTAACTGTTAAAGATGGATCTTTAGTTGATAAAGATGGAATTAT
TTCTTTAGGTGGAGCTACTGGAGCTCTAATTAGAGGAACAAATGTACAAGTTGTTTATGGTGGAGAACAAGAAGCTATTA
AACCTCGTATGATTAAAATCTTAGATGAACAAAGAAAATCTAAAAAATAG

Upstream 100 bases:

>100_bases
TATTTTTCTATCTTTATTTGGATTTGTTATGGCGTTTTCAGGATTAATGATGTTAGTCTAATTTTGCACCATTAAAAACA
AATTATGAAAGGGAAAAAAG

Downstream 100 bases:

>100_bases
TAATATTTAAAAATAAAATGCTTATCGTAAGATAAGCATTTTTTATTTGAAGTAGTTTTCTATTTTTTCTTTTAAATATT
CTTTTTCTTCAGTATTAGTG

Product: glucose-specific PTS system IIABC component

Products: NA

Alternate protein names: EIICBA-Glc 2; Glucoside permease IIC component; PTS system glucoside-specific EIIC component; Glucoside-specific phosphotransferase enzyme IIB component; PTS system glucoside-specific EIIB component; Glucoside-specific phosphotransferase enzyme IIA component; PTS system glucoside-specific EIIA component [H]

Number of amino acids: Translated: 709; Mature: 709

Protein sequence:

>709_residues
MKSFSSIEWKSSAKNSWEMFKKGCGNIMPTLSKLSKAFLLPIALLPIAGVFLGVGSAIASNSVDGTFGYFLGSLLNKMGD
VCFGNLPVLFCISVALAYTKDSGIAAITAVVGFLVMNGMQAALLHTQQLNIDTTNLWVQTSIDENGKAVWEHFTSVVVDA
NGKGKINWADSSINLQNIMAWGIGSSEDNLRMVNSNFELIDGAGSVYYSLLWINKIPNGLITSNIGVNSLNTGVFAGIFV
GAIAAKCYNKFHQTQLPSAISFFSGTKLVPIVTFVAVIPLSFIFMFIWPYVGMGLAAFGQASGKLPGGLDSFIYEVAERS
LVPFGLHHVFYAPLWWTNAGGSIAEAFNKATPEQQAEFVKIWSANHSVLAETLLNGVSEGLKFQTIRNMISSDYPDLWKS
MGDQTMAYSVIAKINVLNFTDLEALGLNIGRFQSGKFGFMLLGLPMAGLAMWLNVPKENRKEVMGIYFSAAFTCFLTGIT
EPIEYTFLFLAPWLFYGVHMPLAAISFLFAGLFKTHVSMTVSGGFIDYIVFGVIPYFGSKAMSAKSAFAILGVSAAMAPA
YFFGFYFAVKYGNVMVPGRDGSTNVQLATKAEYKASKGLNVDGSKIEEGKSTKASAKDAVEEARIQKATKIIEFLGGEAN
IVDVDACASRLRLTVKDGSLVDKDGIISLGGATGALIRGTNVQVVYGGEQEAIKPRMIKILDEQRKSKK

Sequences:

>Translated_709_residues
MKSFSSIE*KSSAKNS*EMFKKGCGNIMPTLSKLSKAFLLPIALLPIAGVFLGVGSAIASNSVDGTFGYFLGSLLNKMGD
VCFGNLPVLFCISVALAYTKDSGIAAITAVVGFLVMNGMQAALLHTQQLNIDTTNL*VQTSIDENGKAV*EHFTSVVVDA
NGKGKIN*ADSSINLQNIMA*GIGSSEDNLRMVNSNFELIDGAGSVYYSLL*INKIPNGLITSNIGVNSLNTGVFAGIFV
GAIAAKCYNKFHQTQLPSAISFFSGTKLVPIVTFVAVIPLSFIFMFI*PYVGMGLAAFGQASGKLPGGLDSFIYEVAERS
LVPFGLHHVFYAPL*WTNAGGSIAEAFNKATPEQQAEFVKI*SANHSVLAETLLNGVSEGLKFQTIRNMISSDYPDL*KS
MGDQTMAYSVIAKINVLNFTDLEALGLNIGRFQSGKFGFMLLGLPMAGLAM*LNVPKENRKEVMGIYFSAAFTCFLTGIT
EPIEYTFLFLAP*LFYGVHMPLAAISFLFAGLFKTHVSMTVSGGFIDYIVFGVIPYFGSKAMSAKSAFAILGVSAAMAPA
YFFGFYFAVKYGNVMVPGRDGSTNVQLATKAEYKASKGLNVDGSKIEEGKSTKASAKDAVEEARIQKATKIIEFLGGEAN
IVDVDACASRLRLTVKDGSLVDKDGIISLGGATGALIRGTNVQVVYGGEQEAIKPRMIKILDEQRKSKK
>Mature_709_residues
MKSFSSIE*KSSAKNS*EMFKKGCGNIMPTLSKLSKAFLLPIALLPIAGVFLGVGSAIASNSVDGTFGYFLGSLLNKMGD
VCFGNLPVLFCISVALAYTKDSGIAAITAVVGFLVMNGMQAALLHTQQLNIDTTNL*VQTSIDENGKAV*EHFTSVVVDA
NGKGKIN*ADSSINLQNIMA*GIGSSEDNLRMVNSNFELIDGAGSVYYSLL*INKIPNGLITSNIGVNSLNTGVFAGIFV
GAIAAKCYNKFHQTQLPSAISFFSGTKLVPIVTFVAVIPLSFIFMFI*PYVGMGLAAFGQASGKLPGGLDSFIYEVAERS
LVPFGLHHVFYAPL*WTNAGGSIAEAFNKATPEQQAEFVKI*SANHSVLAETLLNGVSEGLKFQTIRNMISSDYPDL*KS
MGDQTMAYSVIAKINVLNFTDLEALGLNIGRFQSGKFGFMLLGLPMAGLAM*LNVPKENRKEVMGIYFSAAFTCFLTGIT
EPIEYTFLFLAP*LFYGVHMPLAAISFLFAGLFKTHVSMTVSGGFIDYIVFGVIPYFGSKAMSAKSAFAILGVSAAMAPA
YFFGFYFAVKYGNVMVPGRDGSTNVQLATKAEYKASKGLNVDGSKIEEGKSTKASAKDAVEEARIQKATKIIEFLGGEAN
IVDVDACASRLRLTVKDGSLVDKDGIISLGGATGALIRGTNVQVVYGGEQEAIKPRMIKILDEQRKSKK

Specific function: The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. This

COG id: COG1263

COG function: function code G; Phosphotransferase system IIC components, glucose/maltose/N-acetylglucosamine-specific

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIC type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1787343, Length=263, Percent_Identity=34.2205323193916, Blast_Score=129, Evalue=5e-31,
Organism=Escherichia coli, GI1786894, Length=271, Percent_Identity=32.8413284132841, Blast_Score=115, Evalue=1e-26,
Organism=Escherichia coli, GI1787908, Length=267, Percent_Identity=32.2097378277154, Blast_Score=113, Evalue=4e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011055
- InterPro:   IPR018113
- InterPro:   IPR001127
- InterPro:   IPR001996
- InterPro:   IPR003352
- InterPro:   IPR013013
- InterPro:   IPR011535
- InterPro:   IPR011299 [H]

Pfam domain/function: PF00358 PTS_EIIA_1; PF00367 PTS_EIIB; PF02378 PTS_EIIC [H]

EC number: =2.7.1.69 [H]

Molecular weight: Translated: 73924; Mature: 73924

Theoretical pI: Translated: 8.65; Mature: 8.65

Prosite motif: PS51098 PTS_EIIB_TYPE_1 ; PS51103 PTS_EIIC_TYPE_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKSFSSIEKSSAKNSEMFKKGCGNIMPTLSKLSKAFLLPIALLPIAGVFLGVGSAIASNS
CCCHHHHHHHCCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
VDGTFGYFLGSLLNKMGDVCFGNLPVLFCISVALAYTKDSGIAAITAVVGFLVMNGMQAA
CCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCHHHH
LLHTQQLNIDTTNLVQTSIDENGKAVEHFTSVVVDANGKGKINADSSINLQNIMAGIGSS
HHHHHCCCCCHHHHHHHCCCCCCHHHHHHHHHEEECCCCCCCCCCCCCCHHHHHHHCCCC
EDNLRMVNSNFELIDGAGSVYYSLLINKIPNGLITSNIGVNSLNTGVFAGIFVGAIAAKC
CCCEEEECCCCEEECCCHHHHHHHHHHHCCCCCEECCCCCCCCCHHHHHHHHHHHHHHHH
YNKFHQTQLPSAISFFSGTKLVPIVTFVAVIPLSFIFMFIPYVGMGLAAFGQASGKLPGG
HHHHHHHCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
LDSFIYEVAERSLVPFGLHHVFYAPLWTNAGGSIAEAFNKATPEQQAEFVKISANHSVLA
HHHHHHHHHHCCCCCCCHHHHHEEHHCCCCCCHHHHHHHCCCCHHCCCEEEEECCHHHHH
ETLLNGVSEGLKFQTIRNMISSDYPDLKSMGDQTMAYSVIAKINVLNFTDLEALGLNIGR
HHHHHHHHHCCHHHHHHHHHHCCCCCHHHCCCHHHHHHHHHHHEEECCCCHHHHCCCCCC
FQSGKFGFMLLGLPMAGLAMLNVPKENRKEVMGIYFSAAFTCFLTGITEPIEYTFLFLAP
CCCCCCCEEEECCCHHHHHEECCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHH
LFYGVHMPLAAISFLFAGLFKTHVSMTVSGGFIDYIVFGVIPYFGSKAMSAKSAFAILGV
HHHHHHHHHHHHHHHHHHHHHHHHEEEECCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHH
SAAMAPAYFFGFYFAVKYGNVMVPGRDGSTNVQLATKAEYKASKGLNVDGSKIEEGKSTK
HHHHHHHHHHHHHHHHHCCCEEECCCCCCCCEEEEECCHHHHCCCCCCCCCCCCCCCCCC
ASAKDAVEEARIQKATKIIEFLGGEANIVDVDACASRLRLTVKDGSLVDKDGIISLGGAT
HHHHHHHHHHHHHHHHHHHHHHCCCCCEEEHHHHHHHCEEEECCCCEECCCCEEEECCCC
GALIRGTNVQVVYGGEQEAIKPRMIKILDEQRKSKK
CEEEECCEEEEEECCCHHCCCHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKSFSSIEKSSAKNSEMFKKGCGNIMPTLSKLSKAFLLPIALLPIAGVFLGVGSAIASNS
CCCHHHHHHHCCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
VDGTFGYFLGSLLNKMGDVCFGNLPVLFCISVALAYTKDSGIAAITAVVGFLVMNGMQAA
CCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCHHHH
LLHTQQLNIDTTNLVQTSIDENGKAVEHFTSVVVDANGKGKINADSSINLQNIMAGIGSS
HHHHHCCCCCHHHHHHHCCCCCCHHHHHHHHHEEECCCCCCCCCCCCCCHHHHHHHCCCC
EDNLRMVNSNFELIDGAGSVYYSLLINKIPNGLITSNIGVNSLNTGVFAGIFVGAIAAKC
CCCEEEECCCCEEECCCHHHHHHHHHHHCCCCCEECCCCCCCCCHHHHHHHHHHHHHHHH
YNKFHQTQLPSAISFFSGTKLVPIVTFVAVIPLSFIFMFIPYVGMGLAAFGQASGKLPGG
HHHHHHHCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
LDSFIYEVAERSLVPFGLHHVFYAPLWTNAGGSIAEAFNKATPEQQAEFVKISANHSVLA
HHHHHHHHHHCCCCCCCHHHHHEEHHCCCCCCHHHHHHHCCCCHHCCCEEEEECCHHHHH
ETLLNGVSEGLKFQTIRNMISSDYPDLKSMGDQTMAYSVIAKINVLNFTDLEALGLNIGR
HHHHHHHHHCCHHHHHHHHHHCCCCCHHHCCCHHHHHHHHHHHEEECCCCHHHHCCCCCC
FQSGKFGFMLLGLPMAGLAMLNVPKENRKEVMGIYFSAAFTCFLTGITEPIEYTFLFLAP
CCCCCCCEEEECCCHHHHHEECCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHH
LFYGVHMPLAAISFLFAGLFKTHVSMTVSGGFIDYIVFGVIPYFGSKAMSAKSAFAILGV
HHHHHHHHHHHHHHHHHHHHHHHHEEEECCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHH
SAAMAPAYFFGFYFAVKYGNVMVPGRDGSTNVQLATKAEYKASKGLNVDGSKIEEGKSTK
HHHHHHHHHHHHHHHHHCCCEEECCCCCCCCEEEEECCHHHHCCCCCCCCCCCCCCCCCC
ASAKDAVEEARIQKATKIIEFLGGEANIVDVDACASRLRLTVKDGSLVDKDGIISLGGAT
HHHHHHHHHHHHHHHHHHHHHHCCCCCEEEHHHHHHHCEEEECCCCEECCCCEEEECCCC
GALIRGTNVQVVYGGEQEAIKPRMIKILDEQRKSKK
CEEEECCEEEEEECCCHHCCCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8602153; 10974121 [H]