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The map label for this gene is folD

Identifier: 50364982

GI number: 50364982

Start: 189963

End: 190823

Strand: Direct

Name: folD

Synonym: Mfl167

Alternate gene names: 50364982

Gene position: 189963-190823 (Clockwise)

Preceding gene: 50364981

Following gene: 50364984

Centisome position: 23.95

GC content: 25.78

Gene sequence:

>861_bases
ATGATTATTTTAGACGGAAAAAAAGTAGCATCAAGAAGAAAAGAAGAGTTAACTGCTAAAATTTCAAAATATCACAATAA
AGGGTTAAGAAAACCTAAATTAGTTGTTATTATGGTTGGTAATGATCCTGCTAGTGAAGTTTATGTTTCTCATAAAATTA
AAGTAGCTAATGAAGTTGGGATTTATTCAGAGCTTTTAAGATTTGATAAAGATATTAAAAAAGAAGAGTTGTACAATAAA
ATAAATGAATTAAATAATGATCAAAATATAGATGGTATTTTACTTCAATTACCATTACCTATTGATTTTATTGAAGAAGA
TTATTTACAAGCAATCACTCCATCAAAAGATGTTGATGGGTTCCACTATATTAACCAAGGTAAAATGTTGCAAGGTTATG
ATACAGTTTATCCGTGTACACCTTTAGGAATAATTAACTTATTAGAAGAATACAATATAAGTGTTAAGAATAAAGACATA
ACTTTAATTGGAACAAGTAATATAGTTGGTAAACCTTTAGGCATGATGTTATTGAATAATCAAGCAACTATAACAATGTG
TAATAAAAATACTAAAAATATTAAAAATCATACAATTAATGCAGATATTATTATTAGTGCTACAGGAAAACAATTTATTA
TTACTGAAGATATGATAAAAAAAGATGCAGTAATTATTGATGTTGGAATCATCAGAGATCCAGTTACAAATAAACTTGTT
GGTGATGTTGATTTTGAAAAAGTTAAACTAAAATCTAGTTATATAACTCCTGTTCCAGGTGGTGTTGGTCCAATGACTGT
TATTACATTAATGGAAAATACATTTGTGCTATATGAAAAACATATTAATAAATCAAAATAA

Upstream 100 bases:

>100_bases
AATTAGGAATATCAAATACATTATTAGCATTTATTAAACCATCAAGAAAAACAAACTTTAAATATTTAGAAGAAGACGTA
ACTATAGGGTAGGTGATATT

Downstream 100 bases:

>100_bases
AAACGCTTAATTGGCGTTTTTTTATACAAAAATTAAAAAATAATAAAAATTATTAAATTTTATTTACAACTTATTTTTTA
TATGATAAGATTTTTATTGT

Product: methylenetetrahydrofolate dehydrogenase

Products: NA

Alternate protein names: Methylenetetrahydrofolate dehydrogenase; Methenyltetrahydrofolate cyclohydrolase

Number of amino acids: Translated: 286; Mature: 286

Protein sequence:

>286_residues
MIILDGKKVASRRKEELTAKISKYHNKGLRKPKLVVIMVGNDPASEVYVSHKIKVANEVGIYSELLRFDKDIKKEELYNK
INELNNDQNIDGILLQLPLPIDFIEEDYLQAITPSKDVDGFHYINQGKMLQGYDTVYPCTPLGIINLLEEYNISVKNKDI
TLIGTSNIVGKPLGMMLLNNQATITMCNKNTKNIKNHTINADIIISATGKQFIITEDMIKKDAVIIDVGIIRDPVTNKLV
GDVDFEKVKLKSSYITPVPGGVGPMTVITLMENTFVLYEKHINKSK

Sequences:

>Translated_286_residues
MIILDGKKVASRRKEELTAKISKYHNKGLRKPKLVVIMVGNDPASEVYVSHKIKVANEVGIYSELLRFDKDIKKEELYNK
INELNNDQNIDGILLQLPLPIDFIEEDYLQAITPSKDVDGFHYINQGKMLQGYDTVYPCTPLGIINLLEEYNISVKNKDI
TLIGTSNIVGKPLGMMLLNNQATITMCNKNTKNIKNHTINADIIISATGKQFIITEDMIKKDAVIIDVGIIRDPVTNKLV
GDVDFEKVKLKSSYITPVPGGVGPMTVITLMENTFVLYEKHINKSK
>Mature_286_residues
MIILDGKKVASRRKEELTAKISKYHNKGLRKPKLVVIMVGNDPASEVYVSHKIKVANEVGIYSELLRFDKDIKKEELYNK
INELNNDQNIDGILLQLPLPIDFIEEDYLQAITPSKDVDGFHYINQGKMLQGYDTVYPCTPLGIINLLEEYNISVKNKDI
TLIGTSNIVGKPLGMMLLNNQATITMCNKNTKNIKNHTINADIIISATGKQFIITEDMIKKDAVIIDVGIIRDPVTNKLV
GDVDFEKVKLKSSYITPVPGGVGPMTVITLMENTFVLYEKHINKSK

Specific function: Catalyzes the oxidation of 5,10- methylenetetrahydrofolate to 5,10-methenyltetrahydrofolate and then the hydrolysis of 5,10-methenyltetrahydrofolate to 10- formyltetrahydrofolate

COG id: COG0190

COG function: function code H; 5,10-methylene-tetrahydrofolate dehydrogenase/Methenyl tetrahydrofolate cyclohydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tetrahydrofolate dehydrogenase/cyclohydrolase family

Homologues:

Organism=Homo sapiens, GI94721354, Length=306, Percent_Identity=44.4444444444444, Blast_Score=229, Evalue=3e-60,
Organism=Homo sapiens, GI222418558, Length=293, Percent_Identity=42.320819112628, Blast_Score=222, Evalue=2e-58,
Organism=Homo sapiens, GI222136639, Length=295, Percent_Identity=35.2542372881356, Blast_Score=190, Evalue=1e-48,
Organism=Escherichia coli, GI1786741, Length=273, Percent_Identity=47.2527472527472, Blast_Score=246, Evalue=2e-66,
Organism=Caenorhabditis elegans, GI17568735, Length=251, Percent_Identity=39.4422310756972, Blast_Score=186, Evalue=1e-47,
Organism=Saccharomyces cerevisiae, GI6319558, Length=292, Percent_Identity=40.4109589041096, Blast_Score=205, Evalue=5e-54,
Organism=Saccharomyces cerevisiae, GI6321643, Length=300, Percent_Identity=35.3333333333333, Blast_Score=178, Evalue=1e-45,
Organism=Saccharomyces cerevisiae, GI6322933, Length=256, Percent_Identity=26.171875, Blast_Score=73, Evalue=6e-14,
Organism=Drosophila melanogaster, GI17136816, Length=298, Percent_Identity=40.9395973154362, Blast_Score=214, Evalue=6e-56,
Organism=Drosophila melanogaster, GI17136818, Length=298, Percent_Identity=40.9395973154362, Blast_Score=214, Evalue=8e-56,
Organism=Drosophila melanogaster, GI62472483, Length=284, Percent_Identity=37.6760563380282, Blast_Score=180, Evalue=1e-45,
Organism=Drosophila melanogaster, GI45551871, Length=284, Percent_Identity=37.6760563380282, Blast_Score=180, Evalue=1e-45,
Organism=Drosophila melanogaster, GI24645718, Length=284, Percent_Identity=37.6760563380282, Blast_Score=179, Evalue=2e-45,
Organism=Drosophila melanogaster, GI17137370, Length=284, Percent_Identity=37.6760563380282, Blast_Score=179, Evalue=2e-45,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): FOLD_MESFL (Q6F1V0)

Other databases:

- EMBL:   AE017263
- RefSeq:   YP_053407.1
- HSSP:   P11586
- ProteinModelPortal:   Q6F1V0
- SMR:   Q6F1V0
- GeneID:   2898248
- GenomeReviews:   AE017263_GR
- KEGG:   mfl:Mfl167
- HOGENOM:   HBG328751
- OMA:   GIDNTKV
- ProtClustDB:   CLSK745873
- BioCyc:   MFLO265311:MFL167-MONOMER
- BRENDA:   1.5.1.5
- BRENDA:   3.5.4.9
- GO:   GO:0005488
- HAMAP:   MF_01576
- InterPro:   IPR016040
- InterPro:   IPR000672
- InterPro:   IPR020630
- InterPro:   IPR020867
- InterPro:   IPR020631
- Gene3D:   G3DSA:3.40.50.720
- PRINTS:   PR00085

Pfam domain/function: PF00763 THF_DHG_CYH; PF02882 THF_DHG_CYH_C

EC number: =1.5.1.5; =3.5.4.9

Molecular weight: Translated: 32105; Mature: 32105

Theoretical pI: Translated: 8.56; Mature: 8.56

Prosite motif: PS00766 THF_DHG_CYH_1; PS00767 THF_DHG_CYH_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIILDGKKVASRRKEELTAKISKYHNKGLRKPKLVVIMVGNDPASEVYVSHKIKVANEVG
CEEECCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCHHEEEEEEEEEHHHHH
IYSELLRFDKDIKKEELYNKINELNNDQNIDGILLQLPLPIDFIEEDYLQAITPSKDVDG
HHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCEEEEEECCCHHHHHHHHHHHCCCCCCCCC
FHYINQGKMLQGYDTVYPCTPLGIINLLEEYNISVKNKDITLIGTSNIVGKPLGMMLLNN
EEEECCCCEEECCCCCCCCCHHHHHHHHHHCCCEEECCEEEEEECCCCCCCCCCEEEECC
QATITMCNKNTKNIKNHTINADIIISATGKQFIITEDMIKKDAVIIDVGIIRDPVTNKLV
CEEEEEECCCCCCCCCEECCEEEEEEECCCEEEEEHHHHCCCEEEEEEECCCCCHHHHHC
GDVDFEKVKLKSSYITPVPGGVGPMTVITLMENTFVLYEKHINKSK
CCCCHHEEEEECCCCCCCCCCCCHHHEEEHHHCEEEEEEECCCCCC
>Mature Secondary Structure
MIILDGKKVASRRKEELTAKISKYHNKGLRKPKLVVIMVGNDPASEVYVSHKIKVANEVG
CEEECCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCHHEEEEEEEEEHHHHH
IYSELLRFDKDIKKEELYNKINELNNDQNIDGILLQLPLPIDFIEEDYLQAITPSKDVDG
HHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCEEEEEECCCHHHHHHHHHHHCCCCCCCCC
FHYINQGKMLQGYDTVYPCTPLGIINLLEEYNISVKNKDITLIGTSNIVGKPLGMMLLNN
EEEECCCCEEECCCCCCCCCHHHHHHHHHHCCCEEECCEEEEEECCCCCCCCCCEEEECC
QATITMCNKNTKNIKNHTINADIIISATGKQFIITEDMIKKDAVIIDVGIIRDPVTNKLV
CEEEEEECCCCCCCCCEECCEEEEEEECCCEEEEEHHHHCCCEEEEEEECCCCCHHHHHC
GDVDFEKVKLKSSYITPVPGGVGPMTVITLMENTFVLYEKHINKSK
CCCCHHEEEEECCCCCCCCCCCCHHHEEEHHHCEEEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA