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The map label for this gene is ligA [H]

Identifier: 50364975

GI number: 50364975

Start: 181534

End: 183534

Strand: Direct

Name: ligA [H]

Synonym: Mfl160

Alternate gene names: 50364975

Gene position: 181534-183534 (Clockwise)

Preceding gene: 50364974

Following gene: 50364976

Centisome position: 22.89

GC content: 27.04

Gene sequence:

>2001_bases
ATGACTAAAGAACAAGCAAGCAAATTAATTAATGATTTACGATTAAAACTTAATGAATGAGCAAAAGAATATTATGTTTT
AGATAACCCTAGTGTTGATGATGCTGAATATGACAAAACCATTCATCAACTTCTTGATCTTGAAAATCAATTCCCTGAAT
TAATAACATCTGATTCTATAACTCAAAAAGTTGGCGGAATTGTAAGTGATAAATTTGAAAAGCACACACATAAATATCCA
ATGTTAAGTCTTGGTGACATTTTTTCTTGAGATGAGTTCATTAACTTTAATAAACAAGTTGCAAAAGTTACAGGCACAGA
AAACAATGAATATACTGCAGAACTAAAAATTGATGGATTATCAATATCTTTAATTTATAAAGATGGTGTATTACAAAAAG
GTGTGACTCGTGGTGATGGTAAAGTCGGTGAGAATGTTACAACAAATGTAAAAACAATTAAATCAATACCATTATCAATA
CCTTCAAAAGATGAAATTGAAATTAGAGGAGAAGTTTTTTTAGGTAAAAAAGAATTTGCAAAAATTAATGAAGAAAGATT
ATTGAATGGAGACCAACTTTTTGCCAATCCAAGAAATGCAGCAGCTGGAACTTTAAGACAATTAGATTCTAAAATAGTTG
CTGAAAGAAATCTTGATGCTTATCTTTACTATTATTTCAACGAAAATAACCCAATTAATACACAGTTTGATTCTATTAAT
CAAATAAAGAATTTAGGTTTAAAAATTAATAAGGAAACTAAAATTTGTAAGACATTAGAAGAAGTAAAATTATACATTGA
ATATTACACTGAAAAAAGAAACGAATTAGATTATGAAATAGATGGTATAGTATTTAAACTAAACGATAAAAAATTACAAG
AAGAAGTTGGATACACAGCAAAAACACCAAAATGAGCAATTGCTTATAAATTTCCAGCTGAAGTTAAACAAACTAAATTA
TTAGACATATTTCCCACTGTTGGAAGAACTGGCAAAATTACATACAACGCTAAGCTAGAACCTGTGCAAATTGCTGGAAC
AACAGTTTCTGCAGCATCATTAAACAATGCTGAGTATATAATGGCAAAAGACCTTAGAATAAACTCAATTGTTAAAGTAA
AAAAAGCAGGAGATATTATTCCTGAAGTTATTAGTGCTATTAAAGATGAAAAATTTGATTTATTACCAATTTGAGAAAAA
GATGTTCAATGTCCTGCTTGTAATGAACTTTTAGAGAAAACTTCAACAGAGGTTGATCAATTTTGTGTTAACTTTAACTG
TCCAGCACAAATATTAAGAAGTTTAGAACATTTTGCAAGTAGAGGTGCTGCAAATATAGTTGGTCTTGGTGGTCAAACAA
TTAAAAAATTATTTGAAGAAAAATTAATAACAAATATTGCAGATATTTTTAAAGTTGAAGAACATAAAGAAAATATAATT
AATTTTGAAAAATTTGGAGAAAAAAGTTTTGAAAACTTAATTGCTTCAATTAAAGAATCTAAAAATAATTCATTTGAAAA
AACTTTATTTGGTTTAGGTATTAGACACGTTGGTTCTAAGACTGCTCTAACTTTAGCTCAAATATATAAAAATATTGATA
ATCTAAAAAATGCAACGTATGAAGAATTAAGTTCAATAGACTCAGTTGGAGAAGTGTTGGCTATGTCAATTGTAGATTGA
TTTAAAATTGAGTCTAATTTACAATTAATTAATGAATTAAAATCATTTGATGTTAATTTTGAATATTTGGGTCAAGCTAA
AAATACCGAATCAACAATTAGTGAAAAATCTTTTGTAATAACAGGTACACTATCTGAATCTAGAGATTATTTTAAAGATA
TAATTGAATTAAACAATGGTAAAGTTATAGGTTCTGTTTCAAAAAAAACTGATTATGTTTTAGCAGGGGAAAATGCTGGA
AGTAAATTAACAAAAGCTGAAGAATTAAACGTTAAAGTTATTAATGAAGAAGAGTTCTTTGCAATTCTAAAAGGAGAATA
A

Upstream 100 bases:

>100_bases
AAAACAGACTTTGTTCTGTTTTTTTTGTTGTTTAACTCTTGAAACCATAAAACAAAAACATATAAAAAAGTTTATAATTA
TATAGATATAAAGGAGCATC

Downstream 100 bases:

>100_bases
AATGAAAGATAAAAAGTATTATCAAGAGTTAGCTCAAGATTCAATGTTAAGATTTTCAGATGCGGAAATTGAAGAGATTG
TAGCTAAACAAGAAGATTTA

Product: DNA ligase, polydeoxyribonucleotide synthase NAD+

Products: NA

Alternate protein names: Polydeoxyribonucleotide synthase [NAD+] [H]

Number of amino acids: Translated: 666; Mature: 665

Protein sequence:

>666_residues
MTKEQASKLINDLRLKLNEWAKEYYVLDNPSVDDAEYDKTIHQLLDLENQFPELITSDSITQKVGGIVSDKFEKHTHKYP
MLSLGDIFSWDEFINFNKQVAKVTGTENNEYTAELKIDGLSISLIYKDGVLQKGVTRGDGKVGENVTTNVKTIKSIPLSI
PSKDEIEIRGEVFLGKKEFAKINEERLLNGDQLFANPRNAAAGTLRQLDSKIVAERNLDAYLYYYFNENNPINTQFDSIN
QIKNLGLKINKETKICKTLEEVKLYIEYYTEKRNELDYEIDGIVFKLNDKKLQEEVGYTAKTPKWAIAYKFPAEVKQTKL
LDIFPTVGRTGKITYNAKLEPVQIAGTTVSAASLNNAEYIMAKDLRINSIVKVKKAGDIIPEVISAIKDEKFDLLPIWEK
DVQCPACNELLEKTSTEVDQFCVNFNCPAQILRSLEHFASRGAANIVGLGGQTIKKLFEEKLITNIADIFKVEEHKENII
NFEKFGEKSFENLIASIKESKNNSFEKTLFGLGIRHVGSKTALTLAQIYKNIDNLKNATYEELSSIDSVGEVLAMSIVDW
FKIESNLQLINELKSFDVNFEYLGQAKNTESTISEKSFVITGTLSESRDYFKDIIELNNGKVIGSVSKKTDYVLAGENAG
SKLTKAEELNVKVINEEEFFAILKGE

Sequences:

>Translated_666_residues
MTKEQASKLINDLRLKLNE*AKEYYVLDNPSVDDAEYDKTIHQLLDLENQFPELITSDSITQKVGGIVSDKFEKHTHKYP
MLSLGDIFS*DEFINFNKQVAKVTGTENNEYTAELKIDGLSISLIYKDGVLQKGVTRGDGKVGENVTTNVKTIKSIPLSI
PSKDEIEIRGEVFLGKKEFAKINEERLLNGDQLFANPRNAAAGTLRQLDSKIVAERNLDAYLYYYFNENNPINTQFDSIN
QIKNLGLKINKETKICKTLEEVKLYIEYYTEKRNELDYEIDGIVFKLNDKKLQEEVGYTAKTPK*AIAYKFPAEVKQTKL
LDIFPTVGRTGKITYNAKLEPVQIAGTTVSAASLNNAEYIMAKDLRINSIVKVKKAGDIIPEVISAIKDEKFDLLPI*EK
DVQCPACNELLEKTSTEVDQFCVNFNCPAQILRSLEHFASRGAANIVGLGGQTIKKLFEEKLITNIADIFKVEEHKENII
NFEKFGEKSFENLIASIKESKNNSFEKTLFGLGIRHVGSKTALTLAQIYKNIDNLKNATYEELSSIDSVGEVLAMSIVD*
FKIESNLQLINELKSFDVNFEYLGQAKNTESTISEKSFVITGTLSESRDYFKDIIELNNGKVIGSVSKKTDYVLAGENAG
SKLTKAEELNVKVINEEEFFAILKGE
>Mature_665_residues
TKEQASKLINDLRLKLNE*AKEYYVLDNPSVDDAEYDKTIHQLLDLENQFPELITSDSITQKVGGIVSDKFEKHTHKYPM
LSLGDIFS*DEFINFNKQVAKVTGTENNEYTAELKIDGLSISLIYKDGVLQKGVTRGDGKVGENVTTNVKTIKSIPLSIP
SKDEIEIRGEVFLGKKEFAKINEERLLNGDQLFANPRNAAAGTLRQLDSKIVAERNLDAYLYYYFNENNPINTQFDSINQ
IKNLGLKINKETKICKTLEEVKLYIEYYTEKRNELDYEIDGIVFKLNDKKLQEEVGYTAKTPK*AIAYKFPAEVKQTKLL
DIFPTVGRTGKITYNAKLEPVQIAGTTVSAASLNNAEYIMAKDLRINSIVKVKKAGDIIPEVISAIKDEKFDLLPI*EKD
VQCPACNELLEKTSTEVDQFCVNFNCPAQILRSLEHFASRGAANIVGLGGQTIKKLFEEKLITNIADIFKVEEHKENIIN
FEKFGEKSFENLIASIKESKNNSFEKTLFGLGIRHVGSKTALTLAQIYKNIDNLKNATYEELSSIDSVGEVLAMSIVD*F
KIESNLQLINELKSFDVNFEYLGQAKNTESTISEKSFVITGTLSESRDYFKDIIELNNGKVIGSVSKKTDYVLAGENAGS
KLTKAEELNVKVINEEEFFAILKGE

Specific function: DNA ligase that catalyzes the formation of phosphodiester linkages between 5'-phosphoryl and 3'-hydroxyl groups in double-stranded DNA using NAD as a coenzyme and as the energy source for the reaction. It is essential for DNA replication and repair of dam

COG id: COG0272

COG function: function code L; NAD-dependent DNA ligase (contains BRCT domain type II)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 BRCT domain [H]

Homologues:

Organism=Escherichia coli, GI1788750, Length=653, Percent_Identity=40.7350689127106, Blast_Score=472, Evalue=1e-134,
Organism=Escherichia coli, GI87082305, Length=285, Percent_Identity=24.9122807017544, Blast_Score=80, Evalue=5e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001357
- InterPro:   IPR018239
- InterPro:   IPR004150
- InterPro:   IPR001679
- InterPro:   IPR013839
- InterPro:   IPR013840
- InterPro:   IPR003583
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR010994
- InterPro:   IPR004149 [H]

Pfam domain/function: PF00533 BRCT; PF01653 DNA_ligase_aden; PF03120 DNA_ligase_OB; PF03119 DNA_ligase_ZBD [H]

EC number: =6.5.1.2 [H]

Molecular weight: Translated: 74190; Mature: 74059

Theoretical pI: Translated: 4.99; Mature: 4.99

Prosite motif: PS50172 BRCT ; PS01055 DNA_LIGASE_N1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
0.6 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
1.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKEQASKLINDLRLKLNEAKEYYVLDNPSVDDAEYDKTIHQLLDLENQFPELITSDSIT
CCHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHH
QKVGGIVSDKFEKHTHKYPMLSLGDIFSDEFINFNKQVAKVTGTENNEYTAELKIDGLSI
HHHCCHHHHHHHHHHCCCCCEEHHHHHHHHHHCCCCCEEEEECCCCCCEEEEEEECCEEE
SLIYKDGVLQKGVTRGDGKVGENVTTNVKTIKSIPLSIPSKDEIEIRGEVFLGKKEFAKI
EEEEECCCHHCCCCCCCCCCCCCCCCCHHHHHCCCCCCCCCCCEEEEEEEEECHHHHHHC
NEERLLNGDQLFANPRNAAAGTLRQLDSKIVAERNLDAYLYYYFNENNPINTQFDSINQI
CHHHHCCCCCEECCCCCHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCHHHHHHH
KNLGLKINKETKICKTLEEVKLYIEYYTEKRNELDYEIDGIVFKLNDKKLQEEVGYTAKT
HHCCEEECCCHHHHHHHHHHHHHHHHHHHHCCCCCEEECEEEEEECCHHHHHHCCCCCCC
PKAIAYKFPAEVKQTKLLDIFPTVGRTGKITYNAKLEPVQIAGTTVSAASLNNAEYIMAK
CCCEEEECCCHHHHHEHHEEECCCCCCCEEEECCCCCEEEEECCEEEECCCCCCEEEEEE
DLRINSIVKVKKAGDIIPEVISAIKDEKFDLLPIEKDVQCPACNELLEKTSTEVDQFCVN
CCEECCEEEEHHHCCHHHHHHHHHCCCCCEEECCCCCCCCCHHHHHHHHHCCCHHHHEEC
FNCPAQILRSLEHFASRGAANIVGLGGQTIKKLFEEKLITNIADIFKVEEHKENIINFEK
CCCHHHHHHHHHHHHCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHH
FGEKSFENLIASIKESKNNSFEKTLFGLGIRHVGSKTALTLAQIYKNIDNLKNATYEELS
HCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCHHHHH
SIDSVGEVLAMSIVDFKIESNLQLINELKSFDVNFEYLGQAKNTESTISEKSFVITGTLS
HHHHHHHHHHHHHHHEEECCCHHHHHHHHHCCCCHHHHCCCCCCHHHHCCCCEEEEEECH
ESRDYFKDIIELNNGKVIGSVSKKTDYVLAGENAGSKLTKAEELNVKVINEEEFFAILKG
HHHHHHHHHHHCCCCEEEEECCCCCCEEEECCCCCCCCCCHHCCCEEEECCCCEEEEEEC
E
C
>Mature Secondary Structure 
TKEQASKLINDLRLKLNEAKEYYVLDNPSVDDAEYDKTIHQLLDLENQFPELITSDSIT
CHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHH
QKVGGIVSDKFEKHTHKYPMLSLGDIFSDEFINFNKQVAKVTGTENNEYTAELKIDGLSI
HHHCCHHHHHHHHHHCCCCCEEHHHHHHHHHHCCCCCEEEEECCCCCCEEEEEEECCEEE
SLIYKDGVLQKGVTRGDGKVGENVTTNVKTIKSIPLSIPSKDEIEIRGEVFLGKKEFAKI
EEEEECCCHHCCCCCCCCCCCCCCCCCHHHHHCCCCCCCCCCCEEEEEEEEECHHHHHHC
NEERLLNGDQLFANPRNAAAGTLRQLDSKIVAERNLDAYLYYYFNENNPINTQFDSINQI
CHHHHCCCCCEECCCCCHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCHHHHHHH
KNLGLKINKETKICKTLEEVKLYIEYYTEKRNELDYEIDGIVFKLNDKKLQEEVGYTAKT
HHCCEEECCCHHHHHHHHHHHHHHHHHHHHCCCCCEEECEEEEEECCHHHHHHCCCCCCC
PKAIAYKFPAEVKQTKLLDIFPTVGRTGKITYNAKLEPVQIAGTTVSAASLNNAEYIMAK
CCCEEEECCCHHHHHEHHEEECCCCCCCEEEECCCCCEEEEECCEEEECCCCCCEEEEEE
DLRINSIVKVKKAGDIIPEVISAIKDEKFDLLPIEKDVQCPACNELLEKTSTEVDQFCVN
CCEECCEEEEHHHCCHHHHHHHHHCCCCCEEECCCCCCCCCHHHHHHHHHCCCHHHHEEC
FNCPAQILRSLEHFASRGAANIVGLGGQTIKKLFEEKLITNIADIFKVEEHKENIINFEK
CCCHHHHHHHHHHHHCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHH
FGEKSFENLIASIKESKNNSFEKTLFGLGIRHVGSKTALTLAQIYKNIDNLKNATYEELS
HCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCHHHHH
SIDSVGEVLAMSIVDFKIESNLQLINELKSFDVNFEYLGQAKNTESTISEKSFVITGTLS
HHHHHHHHHHHHHHHEEECCCHHHHHHHHHCCCCHHHHCCCCCCHHHHCCCCEEEEEECH
ESRDYFKDIIELNNGKVIGSVSKKTDYVLAGENAGSKLTKAEELNVKVINEEEFFAILKG
HHHHHHHHHHHCCCCEEEEECCCCCCEEEECCCCCCCCCCHHCCCEEEECCCCEEEEEEC
E
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA