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The map label for this gene is rpoA

Identifier: 50364965

GI number: 50364965

Start: 160910

End: 161863

Strand: Direct

Name: rpoA

Synonym: Mfl150

Alternate gene names: 50364965

Gene position: 160910-161863 (Clockwise)

Preceding gene: 50364964

Following gene: 50364966

Centisome position: 20.29

GC content: 31.34

Gene sequence:

>954_bases
ATGAAACAATTTAATAAACCAGAATTCGGAATTGTAAAAGAATCACCAAATAAATTTTACGGTAAGTTTGAAGCAGCTCC
TCTTGAAAGAGGATTTGCTGTAACTTTAGGTAATGCATTAAGAAGAACATTATTATCATCAACACCGGGAGCAAGTGTTT
TCGCAATTAAAATTGCAGGAGCACAACACGAATTTACTTCAATCTCAGGTATTGAAGAAAATGTAACTCGTATAGTTCTT
AACGTTAAAAAATTAATTTTAAGAATTGATAGCGCGATTTACAATGACGACGAAACTGTTGAACTAAAAGTAGGTACATC
TACAATTGGTCCGGTTACTGCAGGAAGTTTAGTTTTACCAGCAGGAGTAGAGGTTTTAAATAAAGACCTAGTTATTGCAA
ACGTTGCTGAAGGTGGTAATTTAGATTTAGTATTATATGCTAAAAACTCAAGAGGTTACAAAACTTTCAAAGAAAATAAA
GAATTAAAAGATGTAGTACCAGGAATGATTACAATCGATTCAAACTACTCACCAATTATCAAAGTTGCTTATGGATCAAC
TCCAATCAACTTAGGTAAAGCGCAAGATTTTGAAAAATTAATCTTAGAAGTAGAAACAGATGGATCAATCTTAGCATCAG
ATGCTGTTTCATTAGCATCAAAAATTCTAATTTCACACTTTGATGTATTCACTACTTTAGCTGAAGAAGTAGAAGAAGTT
GCAATTATGGGTGTTGAAACTGTTGAAGAAAAAGAGTTAGACAAACCTGTTGAAGAATTAGAGTTTACTCAAAGAAGTTT
AAACTGCTTAAAACGTGCTGGAATCAGTACATTAAGAGAATTAGTTTCTAAAACTGAAGATGAAATTCAAGATATTAGAA
ACTTAGGACGTAAGTCATTAAAAGAAATTAAAGATAAAGTTGCGGCTTTAGAATTAACATTCAAACAAAATTAA

Upstream 100 bases:

>100_bases
GAAGTAACTTCAATTAAGGATTGTACACCAATCCCTCATAATGGAGTTCGTCCTAAAAAACGTCCAAGAAAATAATTATA
GAACAAAGGAGTTTTAGCGA

Downstream 100 bases:

>100_bases
GATTATAAGGAGGTAAATATATGTCATATATTCAAAAACAAGGTAAGAATACTGCATGAAGAGTAGCTTTAATGCGTAAC
TTAACTAGTGAATTAATTGT

Product: DNA-directed RNA polymerase subunit alpha

Products: NA

Alternate protein names: RNAP subunit alpha; RNA polymerase subunit alpha; Transcriptase subunit alpha

Number of amino acids: Translated: 317; Mature: 317

Protein sequence:

>317_residues
MKQFNKPEFGIVKESPNKFYGKFEAAPLERGFAVTLGNALRRTLLSSTPGASVFAIKIAGAQHEFTSISGIEENVTRIVL
NVKKLILRIDSAIYNDDETVELKVGTSTIGPVTAGSLVLPAGVEVLNKDLVIANVAEGGNLDLVLYAKNSRGYKTFKENK
ELKDVVPGMITIDSNYSPIIKVAYGSTPINLGKAQDFEKLILEVETDGSILASDAVSLASKILISHFDVFTTLAEEVEEV
AIMGVETVEEKELDKPVEELEFTQRSLNCLKRAGISTLRELVSKTEDEIQDIRNLGRKSLKEIKDKVAALELTFKQN

Sequences:

>Translated_317_residues
MKQFNKPEFGIVKESPNKFYGKFEAAPLERGFAVTLGNALRRTLLSSTPGASVFAIKIAGAQHEFTSISGIEENVTRIVL
NVKKLILRIDSAIYNDDETVELKVGTSTIGPVTAGSLVLPAGVEVLNKDLVIANVAEGGNLDLVLYAKNSRGYKTFKENK
ELKDVVPGMITIDSNYSPIIKVAYGSTPINLGKAQDFEKLILEVETDGSILASDAVSLASKILISHFDVFTTLAEEVEEV
AIMGVETVEEKELDKPVEELEFTQRSLNCLKRAGISTLRELVSKTEDEIQDIRNLGRKSLKEIKDKVAALELTFKQN
>Mature_317_residues
MKQFNKPEFGIVKESPNKFYGKFEAAPLERGFAVTLGNALRRTLLSSTPGASVFAIKIAGAQHEFTSISGIEENVTRIVL
NVKKLILRIDSAIYNDDETVELKVGTSTIGPVTAGSLVLPAGVEVLNKDLVIANVAEGGNLDLVLYAKNSRGYKTFKENK
ELKDVVPGMITIDSNYSPIIKVAYGSTPINLGKAQDFEKLILEVETDGSILASDAVSLASKILISHFDVFTTLAEEVEEV
AIMGVETVEEKELDKPVEELEFTQRSLNCLKRAGISTLRELVSKTEDEIQDIRNLGRKSLKEIKDKVAALELTFKQN

Specific function: DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates

COG id: COG0202

COG function: function code K; DNA-directed RNA polymerase, alpha subunit/40 kD subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA polymerase alpha chain family

Homologues:

Organism=Escherichia coli, GI1789690, Length=320, Percent_Identity=35.3125, Blast_Score=176, Evalue=2e-45,

Paralogues:

None

Copy number: 850 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 560 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). 6847 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 7,000 Molecules/Cell In: Glucose minimal media

Swissprot (AC and ID): RPOA_MESFL (Q6F1W7)

Other databases:

- EMBL:   AE017263
- RefSeq:   YP_053390.1
- ProteinModelPortal:   Q6F1W7
- GeneID:   2898191
- GenomeReviews:   AE017263_GR
- KEGG:   mfl:Mfl150
- HOGENOM:   HBG430844
- OMA:   EKPIATI
- ProtClustDB:   PRK05182
- BioCyc:   MFLO265311:MFL150-MONOMER
- BRENDA:   2.7.7.6
- HAMAP:   MF_00059
- InterPro:   IPR011261
- InterPro:   IPR011262
- InterPro:   IPR009025
- InterPro:   IPR011263
- InterPro:   IPR011260
- InterPro:   IPR011773
- Gene3D:   G3DSA:2.170.120.12
- ProDom:   PD001179
- SMART:   SM00662
- TIGRFAMs:   TIGR02027

Pfam domain/function: PF01000 RNA_pol_A_bac; PF03118 RNA_pol_A_CTD; PF01193 RNA_pol_L; SSF47789 RNAP_alpha_C; SSF56553 RNAP_insert; SSF55257 RNAP_RBP11-like

EC number: =2.7.7.6

Molecular weight: Translated: 34706; Mature: 34706

Theoretical pI: Translated: 4.88; Mature: 4.88

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
1.3 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKQFNKPEFGIVKESPNKFYGKFEAAPLERGFAVTLGNALRRTLLSSTPGASVFAIKIAG
CCCCCCCCCCEEECCCCHHCCCCCCCCCCCCCEEHHHHHHHHHHHCCCCCCEEEEEEEEC
AQHEFTSISGIEENVTRIVLNVKKLILRIDSAIYNDDETVELKVGTSTIGPVTAGSLVLP
CCCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCEECC
AGVEVLNKDLVIANVAEGGNLDLVLYAKNSRGYKTFKENKELKDVVPGMITIDSNYSPII
CCHHHHCCCEEEEEECCCCCEEEEEEECCCCCCCHHHCCCHHHHHCCCEEEECCCCCCEE
KVAYGSTPINLGKAQDFEKLILEVETDGSILASDAVSLASKILISHFDVFTTLAEEVEEV
EEEECCCCCCCCCCCCHHHHHEEECCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AIMGVETVEEKELDKPVEELEFTQRSLNCLKRAGISTLRELVSKTEDEIQDIRNLGRKSL
HHHCHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHH
KEIKDKVAALELTFKQN
HHHHHHHHHEEEEEECC
>Mature Secondary Structure
MKQFNKPEFGIVKESPNKFYGKFEAAPLERGFAVTLGNALRRTLLSSTPGASVFAIKIAG
CCCCCCCCCCEEECCCCHHCCCCCCCCCCCCCEEHHHHHHHHHHHCCCCCCEEEEEEEEC
AQHEFTSISGIEENVTRIVLNVKKLILRIDSAIYNDDETVELKVGTSTIGPVTAGSLVLP
CCCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCEECC
AGVEVLNKDLVIANVAEGGNLDLVLYAKNSRGYKTFKENKELKDVVPGMITIDSNYSPII
CCHHHHCCCEEEEEECCCCCEEEEEEECCCCCCCHHHCCCHHHHHCCCEEEECCCCCCEE
KVAYGSTPINLGKAQDFEKLILEVETDGSILASDAVSLASKILISHFDVFTTLAEEVEEV
EEEECCCCCCCCCCCCHHHHHEEECCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AIMGVETVEEKELDKPVEELEFTQRSLNCLKRAGISTLRELVSKTEDEIQDIRNLGRKSL
HHHCHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHH
KEIKDKVAALELTFKQN
HHHHHHHHHEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA