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The map label for this gene is polA [H]

Identifier: 50364870

GI number: 50364870

Start: 68965

End: 69918

Strand: Reverse

Name: polA [H]

Synonym: Mfl055

Alternate gene names: 50364870

Gene position: 69918-68965 (Counterclockwise)

Preceding gene: 50364871

Following gene: 50364869

Centisome position: 8.81

GC content: 28.83

Gene sequence:

>954_bases
ATGAATACATTAAATGAAAAACGCACGATATTAATTATCGACGGGTATCATTTGCTACATAAGGGTTACTACGGAACTCT
TAAGAGAAAGAAATTAGCAACTAACAGAGAAGGTGTTCCGATCAATGCGATTTACACTTTTGTTGCAAAAATTAATCAAT
TAGTAAAGAAAAATAACTACTACAGCATCATTGTTACTTTAGACATGGATGAAGGTTGTTGACGTCGAGAATTGTATCCA
GAATATAAAGCAAAAAGAAAAGAAACTCCTGAAGATTTAGTTCCACAAAAACAAATCATTAGAGAATTTTTAACTGCGGC
TAATATTCCATGATATGAAATGCCTAGATACGAAGCTGATGATATTATCGGAACAATTAACAGAATAGCTCTCAAATTAG
ACTATGACGTACATATATTGTCAAATGATAAAGATATTTTCCAGCTTGTTGGTGAAAGAACTACAGTAATTACTAATAGT
AGTAAAGATGACGAGCATATTTTAATTGATCACGAAAAAGTTGTTGAAAAATTTGGATGTCATCCTAGTCAAGTTGCTGA
TATAAAAGCGCTGATGGGTGATCCTAGTGACAATATCAAAGGTGTTAGATATTTACATTATGCTCAAGCAATAGACCTTT
TAAATAAATACGGAAATATTGATAATATTTTTGAACACATTGATGAAATTAATAAAAATATTTCAAAAAGATTAAACGAA
AGCAAAGAATTAATTTTAACCAATAAGAAAATTACAACAATTCAGGATAGATTGCCTATTGGTAGAGTTAATTTAAAACC
AATAAGAGTCAATTGATATGGTTTGAGTAAATTTCTAAAGAAACAAAAAATGTGAGCTTATGTTGCTGATGTTGAAAAAA
TGGCAAGTGAATCAAGAAATAAAAATTCTAAGGTTAATAAAATAAAAAATGAACACATTTCAAAAGAAAAATAA

Upstream 100 bases:

>100_bases
TTTGAAGAAACTCTTCTTGAAATCAAACATTTGCTTAATTAAGTATAATTATATTTAAATTAGATAGTATGTTATAATCT
CTTTGTAAGGATTTAGAAAA

Downstream 100 bases:

>100_bases
TTAATATACTATTTTAAAGGTTTTGGTTATAAGTTTTTTAGTTCTTTCAGTAACACTTTTCTTTGTAGGAAGTTTTTTTG
ACATGCAAATAAATTCTTGA

Product: 5'-3' exonuclease

Products: NA

Alternate protein names: Tth polymerase 1 [H]

Number of amino acids: Translated: 317; Mature: 317

Protein sequence:

>317_residues
MNTLNEKRTILIIDGYHLLHKGYYGTLKRKKLATNREGVPINAIYTFVAKINQLVKKNNYYSIIVTLDMDEGCWRRELYP
EYKAKRKETPEDLVPQKQIIREFLTAANIPWYEMPRYEADDIIGTINRIALKLDYDVHILSNDKDIFQLVGERTTVITNS
SKDDEHILIDHEKVVEKFGCHPSQVADIKALMGDPSDNIKGVRYLHYAQAIDLLNKYGNIDNIFEHIDEINKNISKRLNE
SKELILTNKKITTIQDRLPIGRVNLKPIRVNWYGLSKFLKKQKMWAYVADVEKMASESRNKNSKVNKIKNEHISKEK

Sequences:

>Translated_317_residues
MNTLNEKRTILIIDGYHLLHKGYYGTLKRKKLATNREGVPINAIYTFVAKINQLVKKNNYYSIIVTLDMDEGC*RRELYP
EYKAKRKETPEDLVPQKQIIREFLTAANIP*YEMPRYEADDIIGTINRIALKLDYDVHILSNDKDIFQLVGERTTVITNS
SKDDEHILIDHEKVVEKFGCHPSQVADIKALMGDPSDNIKGVRYLHYAQAIDLLNKYGNIDNIFEHIDEINKNISKRLNE
SKELILTNKKITTIQDRLPIGRVNLKPIRVN*YGLSKFLKKQKM*AYVADVEKMASESRNKNSKVNKIKNEHISKEK
>Mature_317_residues
MNTLNEKRTILIIDGYHLLHKGYYGTLKRKKLATNREGVPINAIYTFVAKINQLVKKNNYYSIIVTLDMDEGC*RRELYP
EYKAKRKETPEDLVPQKQIIREFLTAANIP*YEMPRYEADDIIGTINRIALKLDYDVHILSNDKDIFQLVGERTTVITNS
SKDDEHILIDHEKVVEKFGCHPSQVADIKALMGDPSDNIKGVRYLHYAQAIDLLNKYGNIDNIFEHIDEINKNISKRLNE
SKELILTNKKITTIQDRLPIGRVNLKPIRVN*YGLSKFLKKQKM*AYVADVEKMASESRNKNSKVNKIKNEHISKEK

Specific function: In Addition To Polymerase Activity, This DNA Polymerase Exhibits 3' To 5' And 5' To 3' Exonuclease Activity. It Is Able To Utilize Nicked Circular Duplex DNA As A Template And Can Unwind The Parental DNA Strand From Its Template. [C]

COG id: COG0258

COG function: function code L; 5'-3' exonuclease (including N-terminal domain of PolI)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 5'-3' exonuclease domain [H]

Homologues:

Organism=Escherichia coli, GI1790294, Length=292, Percent_Identity=28.4246575342466, Blast_Score=107, Evalue=1e-24,
Organism=Escherichia coli, GI226510970, Length=237, Percent_Identity=25.3164556962025, Blast_Score=75, Evalue=5e-15,

Paralogues:

None

Copy number: 400 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020046
- InterPro:   IPR020045
- InterPro:   IPR002421
- InterPro:   IPR020047
- InterPro:   IPR019760
- InterPro:   IPR001098
- InterPro:   IPR018320
- InterPro:   IPR002298
- InterPro:   IPR008918
- InterPro:   IPR003583
- InterPro:   IPR012337
- InterPro:   IPR015361 [H]

Pfam domain/function: PF01367 5_3_exonuc; PF02739 5_3_exonuc_N; PF00476 DNA_pol_A; PF09281 Taq-exonuc [H]

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 36173; Mature: 36173

Theoretical pI: Translated: 9.69; Mature: 9.69

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNTLNEKRTILIIDGYHLLHKGYYGTLKRKKLATNREGVPINAIYTFVAKINQLVKKNNY
CCCCCCCCEEEEECCHHHHHCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCC
YSIIVTLDMDEGCRRELYPEYKAKRKETPEDLVPQKQIIREFLTAANIPYEMPRYEADDI
EEEEEEEECCCCHHHHCCHHHHHHCCCCCHHCCCHHHHHHHHHHHCCCCCCCCCCCHHHH
IGTINRIALKLDYDVHILSNDKDIFQLVGERTTVITNSSKDDEHILIDHEKVVEKFGCHP
HHHHHHEEEEECCEEEEECCCHHHHHHHCCCEEEEECCCCCCCEEEECHHHHHHHHCCCH
SQVADIKALMGDPSDNIKGVRYLHYAQAIDLLNKYGNIDNIFEHIDEINKNISKRLNESK
HHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCC
ELILTNKKITTIQDRLPIGRVNLKPIRVNYGLSKFLKKQKMAYVADVEKMASESRNKNSK
EEEEECCCEEEHHHCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHH
VNKIKNEHISKEK
HHHHHHHHCCCCC
>Mature Secondary Structure
MNTLNEKRTILIIDGYHLLHKGYYGTLKRKKLATNREGVPINAIYTFVAKINQLVKKNNY
CCCCCCCCEEEEECCHHHHHCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCC
YSIIVTLDMDEGCRRELYPEYKAKRKETPEDLVPQKQIIREFLTAANIPYEMPRYEADDI
EEEEEEEECCCCHHHHCCHHHHHHCCCCCHHCCCHHHHHHHHHHHCCCCCCCCCCCHHHH
IGTINRIALKLDYDVHILSNDKDIFQLVGERTTVITNSSKDDEHILIDHEKVVEKFGCHP
HHHHHHEEEEECCEEEEECCCHHHHHHHCCCEEEEECCCCCCCEEEECHHHHHHHHCCCH
SQVADIKALMGDPSDNIKGVRYLHYAQAIDLLNKYGNIDNIFEHIDEINKNISKRLNESK
HHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCC
ELILTNKKITTIQDRLPIGRVNLKPIRVNYGLSKFLKKQKMAYVADVEKMASESRNKNSK
EEEEECCCEEEHHHCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHH
VNKIKNEHISKEK
HHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA