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The map label for this gene is trmE [H]

Identifier: 50364829

GI number: 50364829

Start: 18681

End: 20039

Strand: Reverse

Name: trmE [H]

Synonym: Mfl014

Alternate gene names: 50364829

Gene position: 20039-18681 (Counterclockwise)

Preceding gene: 50364830

Following gene: 50364818

Centisome position: 2.53

GC content: 25.83

Gene sequence:

>1359_bases
ATGAACAATTTAAATCAAACTATAGTAGCACCAACAACTAATATATCAACACAAGCTATTTCATTAATTAGAATTAGTGG
TGATGACAGTTTTAACATTATTAATAAATTATTAAAAACAAAAATTAAAGAAGAAAAAAATGTTTGAGTTAGAAAAATGT
TTGATGGTCAAGAGTTGGTTGATGAAGTTGTTATAACATCATTTGTTTCTCCTGCAAGCTTTACAGGTGAAAACGTTGTT
GAAATTGCTTGTCATGGGGGTATTTTAAACACTCAGAGAATAATCAATTTAATTATCAAGAATGGCGCAAGAATGGCTAA
TAAAGGTGAATTTAGTCAAAGAGCATTTTTAAACAATAAAATAGATTTAATTCAAGCTGAGGGTATTAATGATTTAATCT
TCGCTAAAAATGAATTAGCTTTAAAGATTGGTGTTAATAATATGAGTGGCGCCCATAATCAAAGTATTATAAATCTAAAA
GGTAATTTATTAGACATTATTTCTAGAATTCAAGTTTCAATTGATTATCCTGATTATGACGATGTTGAGGGAAGCAGTAT
TCCTGAATTAGCAAAAGCCTTATCTGGCATAAATGAAGAAGTTAACAATTTATTAAAAAGATCAAAAATGGCCATTAAAA
ATACTAATGGTATAAAAACTGCTATTGTTGGTAAAACAAATGTTGGAAAGTCTTCATTATTAAATGCATTATTAAATGAG
GATAAAGCAATAGTCACAGACATTCATGGTACAACAAGAGACATTGTAACTGGAGAAATTAATTTAGAAAATATAAGTTT
AAATTTAATTGATACAGCCGGTATTAGAGAAACAACTGATATTGTAGAAGGTCTTGGAATTGAAAAATCATTAAAAATTA
TTGATGAAGCAGAACTAGTTTTATTTGTTGTTGATTTCAAATCAATTAATGATGAAGAAAATAAAATGATTTTTGACAAA
TTAGAAAACAAAAATTACATACTTGTATTTAATAAAAGTGAATTTATTAATGACATAGAGAGAAATAAAATTAAAGACTT
ACATAATAATTTTGTCTATACTAGCGCGATTAATAATGACATAGATAACTTAATAATGAAAATTGAACAAATGCATATAA
ATGAAGAGATAATAAATAACGATTCTTTAATACTAATTAATTTACAGCAAATAACTCTTGTTGAGCAAGTAAAAGACAGA
CTTGAAAAATCTTTAAACGCAATTATTGGTGGTATGCCAATTGATATTGTTAATGTTGATCTTCATGAAGCTTGAGATTA
TTTAAATCAATTAATTGGTGAGCAATATGACGAAGAAATAATTGATAATATATTCAAAAAGTATTGTTTAGGTAAATAA

Upstream 100 bases:

>100_bases
GTGGTGCTATTGGCGGTAATATTGCTTAATTCAATTAGATTCTTCGGAATCTTTTTTATTGTTAAAATAGTATAATAAGT
AATAAAGACAGGAGATCTAC

Downstream 100 bases:

>100_bases
AAAACAAAGTCTTAATTTTAAACTTTGTTTTTTTATTAATTTTGTTTTAAAATTGCCAACATTCCGTAACAACCTGCATC
ATTTTTTAAATTAGATATTT

Product: tRNA modification GTPase TrmE

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 452; Mature: 452

Protein sequence:

>452_residues
MNNLNQTIVAPTTNISTQAISLIRISGDDSFNIINKLLKTKIKEEKNVWVRKMFDGQELVDEVVITSFVSPASFTGENVV
EIACHGGILNTQRIINLIIKNGARMANKGEFSQRAFLNNKIDLIQAEGINDLIFAKNELALKIGVNNMSGAHNQSIINLK
GNLLDIISRIQVSIDYPDYDDVEGSSIPELAKALSGINEEVNNLLKRSKMAIKNTNGIKTAIVGKTNVGKSSLLNALLNE
DKAIVTDIHGTTRDIVTGEINLENISLNLIDTAGIRETTDIVEGLGIEKSLKIIDEAELVLFVVDFKSINDEENKMIFDK
LENKNYILVFNKSEFINDIERNKIKDLHNNFVYTSAINNDIDNLIMKIEQMHINEEIINNDSLILINLQQITLVEQVKDR
LEKSLNAIIGGMPIDIVNVDLHEAWDYLNQLIGEQYDEEIIDNIFKKYCLGK

Sequences:

>Translated_452_residues
MNNLNQTIVAPTTNISTQAISLIRISGDDSFNIINKLLKTKIKEEKNV*VRKMFDGQELVDEVVITSFVSPASFTGENVV
EIACHGGILNTQRIINLIIKNGARMANKGEFSQRAFLNNKIDLIQAEGINDLIFAKNELALKIGVNNMSGAHNQSIINLK
GNLLDIISRIQVSIDYPDYDDVEGSSIPELAKALSGINEEVNNLLKRSKMAIKNTNGIKTAIVGKTNVGKSSLLNALLNE
DKAIVTDIHGTTRDIVTGEINLENISLNLIDTAGIRETTDIVEGLGIEKSLKIIDEAELVLFVVDFKSINDEENKMIFDK
LENKNYILVFNKSEFINDIERNKIKDLHNNFVYTSAINNDIDNLIMKIEQMHINEEIINNDSLILINLQQITLVEQVKDR
LEKSLNAIIGGMPIDIVNVDLHEA*DYLNQLIGEQYDEEIIDNIFKKYCLGK
>Mature_452_residues
MNNLNQTIVAPTTNISTQAISLIRISGDDSFNIINKLLKTKIKEEKNV*VRKMFDGQELVDEVVITSFVSPASFTGENVV
EIACHGGILNTQRIINLIIKNGARMANKGEFSQRAFLNNKIDLIQAEGINDLIFAKNELALKIGVNNMSGAHNQSIINLK
GNLLDIISRIQVSIDYPDYDDVEGSSIPELAKALSGINEEVNNLLKRSKMAIKNTNGIKTAIVGKTNVGKSSLLNALLNE
DKAIVTDIHGTTRDIVTGEINLENISLNLIDTAGIRETTDIVEGLGIEKSLKIIDEAELVLFVVDFKSINDEENKMIFDK
LENKNYILVFNKSEFINDIERNKIKDLHNNFVYTSAINNDIDNLIMKIEQMHINEEIINNDSLILINLQQITLVEQVKDR
LEKSLNAIIGGMPIDIVNVDLHEA*DYLNQLIGEQYDEEIIDNIFKKYCLGK

Specific function: Exhibits a very high intrinsic GTPase hydrolysis rate. Involved in the addition of a carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of certain tRNAs, forming tRNA- cmnm(5)s(2)U34 [H]

COG id: COG0486

COG function: function code R; Predicted GTPase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 G (guanine nucleotide-binding) domain [H]

Homologues:

Organism=Homo sapiens, GI193082991, Length=467, Percent_Identity=23.7687366167024, Blast_Score=144, Evalue=2e-34,
Organism=Homo sapiens, GI193082993, Length=456, Percent_Identity=24.3421052631579, Blast_Score=140, Evalue=2e-33,
Organism=Homo sapiens, GI193082988, Length=499, Percent_Identity=23.4468937875752, Blast_Score=136, Evalue=4e-32,
Organism=Escherichia coli, GI2367268, Length=460, Percent_Identity=30.8695652173913, Blast_Score=204, Evalue=7e-54,
Organism=Escherichia coli, GI87082120, Length=134, Percent_Identity=35.0746268656716, Blast_Score=77, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI17507259, Length=456, Percent_Identity=28.0701754385965, Blast_Score=164, Evalue=9e-41,
Organism=Saccharomyces cerevisiae, GI6323665, Length=499, Percent_Identity=28.8577154308617, Blast_Score=164, Evalue=3e-41,
Organism=Drosophila melanogaster, GI45550813, Length=471, Percent_Identity=27.3885350318471, Blast_Score=167, Evalue=1e-41,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018948
- InterPro:   IPR002917
- InterPro:   IPR001806
- InterPro:   IPR005225
- InterPro:   IPR004520 [H]

Pfam domain/function: PF01926 MMR_HSR1; PF10396 TrmE_N [H]

EC number: NA

Molecular weight: Translated: 50212; Mature: 50212

Theoretical pI: Translated: 4.58; Mature: 4.58

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNNLNQTIVAPTTNISTQAISLIRISGDDSFNIINKLLKTKIKEEKNVVRKMFDGQELVD
CCCCCCEEEECCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHH
EVVITSFVSPASFTGENVVEIACHGGILNTQRIINLIIKNGARMANKGEFSQRAFLNNKI
HHHHHHHCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHCCCCCCCCCCHHHHHHHCCCE
DLIQAEGINDLIFAKNELALKIGVNNMSGAHNQSIINLKGNLLDIISRIQVSIDYPDYDD
EEEEECCCCCEEEECCCEEEEEECCCCCCCCCCEEEEECCHHHHHHHHHEEEECCCCCCC
VEGSSIPELAKALSGINEEVNNLLKRSKMAIKNTNGIKTAIVGKTNVGKSSLLNALLNED
CCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCHHHHHHHHHCCC
KAIVTDIHGTTRDIVTGEINLENISLNLIDTAGIRETTDIVEGLGIEKSLKIIDEAELVL
CEEEEECCCCCCCEEEEEEEEEEEEEEEEECCCCCHHHHHHHCCCCCHHHHHCCCCEEEE
FVVDFKSINDEENKMIFDKLENKNYILVFNKSEFINDIERNKIKDLHNNFVYTSAINNDI
EEEECCCCCCCCCCCHHHHHCCCCEEEEECCHHHHHHHHHHHHHHHHCCEEEEEHHCCCH
DNLIMKIEQMHINEEIINNDSLILINLQQITLVEQVKDRLEKSLNAIIGGMPIDIVNVDL
HHHHHHHHHHHCCHHHHCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECC
HEADYLNQLIGEQYDEEIIDNIFKKYCLGK
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MNNLNQTIVAPTTNISTQAISLIRISGDDSFNIINKLLKTKIKEEKNVVRKMFDGQELVD
CCCCCCEEEECCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHH
EVVITSFVSPASFTGENVVEIACHGGILNTQRIINLIIKNGARMANKGEFSQRAFLNNKI
HHHHHHHCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHCCCCCCCCCCHHHHHHHCCCE
DLIQAEGINDLIFAKNELALKIGVNNMSGAHNQSIINLKGNLLDIISRIQVSIDYPDYDD
EEEEECCCCCEEEECCCEEEEEECCCCCCCCCCEEEEECCHHHHHHHHHEEEECCCCCCC
VEGSSIPELAKALSGINEEVNNLLKRSKMAIKNTNGIKTAIVGKTNVGKSSLLNALLNED
CCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCHHHHHHHHHCCC
KAIVTDIHGTTRDIVTGEINLENISLNLIDTAGIRETTDIVEGLGIEKSLKIIDEAELVL
CEEEEECCCCCCCEEEEEEEEEEEEEEEEECCCCCHHHHHHHCCCCCHHHHHCCCCEEEE
FVVDFKSINDEENKMIFDKLENKNYILVFNKSEFINDIERNKIKDLHNNFVYTSAINNDI
EEEECCCCCCCCCCCHHHHHCCCCEEEEECCHHHHHHHHHHHHHHHHCCEEEEEHHCCCH
DNLIMKIEQMHINEEIINNDSLILINLQQITLVEQVKDRLEKSLNAIIGGMPIDIVNVDL
HHHHHHHHHHHCCHHHHCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECC
HEADYLNQLIGEQYDEEIIDNIFKKYCLGK
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA