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The map label for this gene is gloA [H]

Identifier: 50085322

GI number: 50085322

Start: 2183391

End: 2183792

Strand: Reverse

Name: gloA [H]

Synonym: ACIAD2213

Alternate gene names: 50085322

Gene position: 2183792-2183391 (Counterclockwise)

Preceding gene: 50085323

Following gene: 50085321

Centisome position: 60.68

GC content: 40.3

Gene sequence:

>402_bases
ATGCGAATGCTGCATACAATGTTGCGAGTAGGCAATTTAGAACAATCAATCAAGTTCTATACTGAAGTCTTAGGTATGAC
ATTACTTCGTAAACGTGATTATGAGGAAGGCCGCTTTACTTTAGCATTTGTTGGCTATGGTCAAGAATCCGATCATACAG
TACTCGAACTTACACATAACTGGGATACTTCAAGTTATGACCTAGGAAATGGATATGGTCATATCGCGATTGGTGTTGAA
GATGCCTACAAAGCATGCGATCTGATTAAAGAACGTGGCGGTAATGTCGTTCGTGAAGCGGGCCCAATGAAAGGTGGCGT
GACTGTTATTGCATTTGTTGAAGATCCAGATGGCTACAAAATTGAGCTGATTCAACAAGATGAAAATGCACGTAATAATT
GA

Upstream 100 bases:

>100_bases
TCATTGGAATTCCAATTTTAGTGCTTGTTCTTTGGGTTGCAAAATTACTTAAGGAACATGAGGCTAAACAGACAAGTATT
AACAAGACAGGTGATTAATC

Downstream 100 bases:

>100_bases
TTTTAAAGTAAAAGTTTTTAAATAAATACCCAACATTTTATTTAAAAACTGGATTTATTTAATCAAACCAGTAACATACG
ATTTACCATGCAGTAAATTG

Product: lactoylglutathione lyase

Products: NA

Alternate protein names: Aldoketomutase; Glyoxalase I; Glx I; Ketone-aldehyde mutase; Methylglyoxalase; S-D-lactoylglutathione methylglyoxal lyase [H]

Number of amino acids: Translated: 133; Mature: 133

Protein sequence:

>133_residues
MRMLHTMLRVGNLEQSIKFYTEVLGMTLLRKRDYEEGRFTLAFVGYGQESDHTVLELTHNWDTSSYDLGNGYGHIAIGVE
DAYKACDLIKERGGNVVREAGPMKGGVTVIAFVEDPDGYKIELIQQDENARNN

Sequences:

>Translated_133_residues
MRMLHTMLRVGNLEQSIKFYTEVLGMTLLRKRDYEEGRFTLAFVGYGQESDHTVLELTHNWDTSSYDLGNGYGHIAIGVE
DAYKACDLIKERGGNVVREAGPMKGGVTVIAFVEDPDGYKIELIQQDENARNN
>Mature_133_residues
MRMLHTMLRVGNLEQSIKFYTEVLGMTLLRKRDYEEGRFTLAFVGYGQESDHTVLELTHNWDTSSYDLGNGYGHIAIGVE
DAYKACDLIKERGGNVVREAGPMKGGVTVIAFVEDPDGYKIELIQQDENARNN

Specific function: Catalyzes the conversion of hemimercaptal, formed from methylglyoxal and glutathione, to S-lactoylglutathione [H]

COG id: COG0346

COG function: function code E; Lactoylglutathione lyase and related lyases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glyoxalase I family [H]

Homologues:

Organism=Homo sapiens, GI118402586, Length=152, Percent_Identity=40.1315789473684, Blast_Score=103, Evalue=6e-23,
Organism=Homo sapiens, GI217330598, Length=133, Percent_Identity=32.3308270676692, Blast_Score=69, Evalue=1e-12,
Organism=Escherichia coli, GI1787940, Length=131, Percent_Identity=61.8320610687023, Blast_Score=178, Evalue=1e-46,
Organism=Caenorhabditis elegans, GI17552228, Length=141, Percent_Identity=31.2056737588652, Blast_Score=78, Evalue=2e-15,
Organism=Saccharomyces cerevisiae, GI6323639, Length=141, Percent_Identity=41.1347517730496, Blast_Score=102, Evalue=2e-23,
Organism=Drosophila melanogaster, GI20129743, Length=144, Percent_Identity=39.5833333333333, Blast_Score=99, Evalue=1e-21,
Organism=Drosophila melanogaster, GI19920404, Length=136, Percent_Identity=33.8235294117647, Blast_Score=78, Evalue=2e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004360
- InterPro:   IPR004361
- InterPro:   IPR018146 [H]

Pfam domain/function: PF00903 Glyoxalase [H]

EC number: =4.4.1.5 [H]

Molecular weight: Translated: 15004; Mature: 15004

Theoretical pI: Translated: 4.80; Mature: 4.80

Prosite motif: PS00934 GLYOXALASE_I_1 ; PS00935 GLYOXALASE_I_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRMLHTMLRVGNLEQSIKFYTEVLGMTLLRKRDYEEGRFTLAFVGYGQESDHTVLELTHN
CHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCCCEEEEEEEC
WDTSSYDLGNGYGHIAIGVEDAYKACDLIKERGGNVVREAGPMKGGVTVIAFVEDPDGYK
CCCCCEECCCCEEEEEEEHHHHHHHHHHHHHCCCCEEECCCCCCCCEEEEEEEECCCCCE
IELIQQDENARNN
EEEEECCCCCCCC
>Mature Secondary Structure
MRMLHTMLRVGNLEQSIKFYTEVLGMTLLRKRDYEEGRFTLAFVGYGQESDHTVLELTHN
CHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCCCEEEEEEEC
WDTSSYDLGNGYGHIAIGVEDAYKACDLIKERGGNVVREAGPMKGGVTVIAFVEDPDGYK
CCCCCEECCCCEEEEEEEHHHHHHHHHHHHHCCCCEEECCCCCCCCEEEEEEEECCCCCE
IELIQQDENARNN
EEEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10761919 [H]