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The map label for this gene is menE [H]

Identifier: 50085226

GI number: 50085226

Start: 2095750

End: 2097201

Strand: Reverse

Name: menE [H]

Synonym: ACIAD2106

Alternate gene names: 50085226

Gene position: 2097201-2095750 (Counterclockwise)

Preceding gene: 50085227

Following gene: 50085225

Centisome position: 58.28

GC content: 39.39

Gene sequence:

>1452_bases
ATGTTGCATAAACTTTCAGATTTTAAATCTAAATTAGAAAATTATCAGTTTCAGCAACCTTATAATATTGCTTTATCTGA
TAAATATCAGACAATCAACTATGCTGATTTACTTAGTGAGGTCAATAAACGACAGGAAATATTAAAACAACTGGATGTAA
AAGTTGTCGCTTTATATTTAGAAAATTCAGTTGATTTAATGCTTTGGGATTTGACATTATTATTTTCTGAAATTCCAACT
ATTATTTTACCGCCATTTTTTTCTTCATCACAACTCAATTATTGTCTTGAGATTTCTCAGGCAGATTTAGTGATTTCAAA
TGATAATCTAAATTTGGATCATCTTGATCTTTATTTTAAGGATGCTTGTTTTTGGCGGTTAAAGTCAGCAAAAGCTGTCG
AATTTCCTCAATCAACACAAAAACTGACATTTACGTCTGGTACCACTGGACATCCCAAAGCGGTATGTCTGGGTAGTGAG
CAAATTCTGACTGTGGCGAAATCCTTAAGTGAAGCGATTGATGAAACAAAACCACAAAAACACCTGGCTCTGATGCCTTT
AGCTGTTCTACTTGAAAATATTGGATGTTATGCAATGTTGTATAGAGGAGCACAGATCGAGCTGGTAAGTGCTTCCGATC
TAGGATTGAGAGGGGCCAGTGGTGTCGATGTTCATCAGTTTTCACATTATTTAAATCAAAGTAAGCCGCATAGTCTGATT
TTAGTACCTCAGTTTTTAAGCGTTTTGGTTGAGGCTGTTTCATCCAAACTTTTACGGGCCGAAGATTATCGCTTTATTGC
GGTAGGTGGCGGTCATGTGAATCCAGATTTGCTTAAACGTGCGCAAACCTTAAATCTGCCAGTCTATGAAGGTTACGGTT
TATCTGAATGTGCTTCTGTGGTTGCCTTAAATACACCAGCGCAGCAACGAGCTGGCAGTGTCGGAAAACCTTTAGCTCAT
GTAGAGGTCAGTATTGCCAATGATGGCGAAATTCTGGTCGCAAATAATCAATGTCTTGGTTATCTAGGGAGCAACCATGC
ACCCGTAAAATACTGGCCGACTGGTGATTTAGGCATGCTTGATGAAGATGGCTATCTTTACGTACATGGCCGTAAGAAAC
ATCAGTTTATTACCAGTTTTGGTCGTAATGTTAACCCTGAATGGGTAGAGGCATTGCTGAGTCAAACTGGCGTAGTGGGT
CAGGCATTTGTCTATGGTGAGGCTTTACCTGAAAACTATGCGCTGCTTTGGCCTGTACATAGTGCGATTAGCAATGATCA
GATTGAGCAGGTCGTGGAACAGGCCAATATTAAATTGCCTGATTATGCCCGTGTCAAACACTGGGTACGTTTAACAGAAC
CGTTTAGTACAACCAATCAACTCGCAACAGCGAATGGTCGATTACGACGTGATGAGATCGTCAGTCGTTACCACAATGTA
TTTTCAGCTTAA

Upstream 100 bases:

>100_bases
CATGTATTTGCAGCAGATGTAAAATTATGCGCATTCAATTTGAATCAGTTACAGGTATTTGAGTCTTTGGGATTAAAACC
TCTTACCGAGGATATCGATC

Downstream 100 bases:

>100_bases
AGGAAGAAATTTATGTCATTTTTTCAACAGCTTCAACAAGCAACCGCTCAAGAAAGACAAAAATTGTTTGCGACTTCACT
GATTGATGATGCCTTGAATA

Product: long-chain-fatty-acid-CoA ligase

Products: NA

Alternate protein names: OSB-CoA synthetase; o-succinylbenzoyl-CoA synthetase [H]

Number of amino acids: Translated: 483; Mature: 483

Protein sequence:

>483_residues
MLHKLSDFKSKLENYQFQQPYNIALSDKYQTINYADLLSEVNKRQEILKQLDVKVVALYLENSVDLMLWDLTLLFSEIPT
IILPPFFSSSQLNYCLEISQADLVISNDNLNLDHLDLYFKDACFWRLKSAKAVEFPQSTQKLTFTSGTTGHPKAVCLGSE
QILTVAKSLSEAIDETKPQKHLALMPLAVLLENIGCYAMLYRGAQIELVSASDLGLRGASGVDVHQFSHYLNQSKPHSLI
LVPQFLSVLVEAVSSKLLRAEDYRFIAVGGGHVNPDLLKRAQTLNLPVYEGYGLSECASVVALNTPAQQRAGSVGKPLAH
VEVSIANDGEILVANNQCLGYLGSNHAPVKYWPTGDLGMLDEDGYLYVHGRKKHQFITSFGRNVNPEWVEALLSQTGVVG
QAFVYGEALPENYALLWPVHSAISNDQIEQVVEQANIKLPDYARVKHWVRLTEPFSTTNQLATANGRLRRDEIVSRYHNV
FSA

Sequences:

>Translated_483_residues
MLHKLSDFKSKLENYQFQQPYNIALSDKYQTINYADLLSEVNKRQEILKQLDVKVVALYLENSVDLMLWDLTLLFSEIPT
IILPPFFSSSQLNYCLEISQADLVISNDNLNLDHLDLYFKDACFWRLKSAKAVEFPQSTQKLTFTSGTTGHPKAVCLGSE
QILTVAKSLSEAIDETKPQKHLALMPLAVLLENIGCYAMLYRGAQIELVSASDLGLRGASGVDVHQFSHYLNQSKPHSLI
LVPQFLSVLVEAVSSKLLRAEDYRFIAVGGGHVNPDLLKRAQTLNLPVYEGYGLSECASVVALNTPAQQRAGSVGKPLAH
VEVSIANDGEILVANNQCLGYLGSNHAPVKYWPTGDLGMLDEDGYLYVHGRKKHQFITSFGRNVNPEWVEALLSQTGVVG
QAFVYGEALPENYALLWPVHSAISNDQIEQVVEQANIKLPDYARVKHWVRLTEPFSTTNQLATANGRLRRDEIVSRYHNV
FSA
>Mature_483_residues
MLHKLSDFKSKLENYQFQQPYNIALSDKYQTINYADLLSEVNKRQEILKQLDVKVVALYLENSVDLMLWDLTLLFSEIPT
IILPPFFSSSQLNYCLEISQADLVISNDNLNLDHLDLYFKDACFWRLKSAKAVEFPQSTQKLTFTSGTTGHPKAVCLGSE
QILTVAKSLSEAIDETKPQKHLALMPLAVLLENIGCYAMLYRGAQIELVSASDLGLRGASGVDVHQFSHYLNQSKPHSLI
LVPQFLSVLVEAVSSKLLRAEDYRFIAVGGGHVNPDLLKRAQTLNLPVYEGYGLSECASVVALNTPAQQRAGSVGKPLAH
VEVSIANDGEILVANNQCLGYLGSNHAPVKYWPTGDLGMLDEDGYLYVHGRKKHQFITSFGRNVNPEWVEALLSQTGVVG
QAFVYGEALPENYALLWPVHSAISNDQIEQVVEQANIKLPDYARVKHWVRLTEPFSTTNQLATANGRLRRDEIVSRYHNV
FSA

Specific function: Converts 2-succinylbenzoate (OSB) to 2-succinylbenzoyl- CoA (OSB-CoA) [H]

COG id: COG1022

COG function: function code I; Long-chain acyl-CoA synthetases (AMP-forming)

Gene ontology:

Cell location: Integral Membrane Protein. Inner Membrane [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family. MenE subfamily [H]

Homologues:

Organism=Escherichia coli, GI1788107, Length=294, Percent_Identity=26.1904761904762, Blast_Score=62, Evalue=7e-11,
Organism=Caenorhabditis elegans, GI17558820, Length=357, Percent_Identity=24.9299719887955, Blast_Score=82, Evalue=6e-16,
Organism=Caenorhabditis elegans, GI32563687, Length=453, Percent_Identity=24.0618101545254, Blast_Score=69, Evalue=5e-12,
Organism=Drosophila melanogaster, GI24648257, Length=369, Percent_Identity=24.9322493224932, Blast_Score=75, Evalue=9e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020845
- InterPro:   IPR000873
- InterPro:   IPR010192
- InterPro:   IPR023015 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: =6.2.1.26 [H]

Molecular weight: Translated: 53852; Mature: 53852

Theoretical pI: Translated: 6.23; Mature: 6.23

Prosite motif: PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLHKLSDFKSKLENYQFQQPYNIALSDKYQTINYADLLSEVNKRQEILKQLDVKVVALYL
CCCCHHHHHHHHHCCCCCCCCEEEECCCCCCCCHHHHHHHHHHHHHHHHHHCCEEEEEEE
ENSVDLMLWDLTLLFSEIPTIILPPFFSSSQLNYCLEISQADLVISNDNLNLDHLDLYFK
CCCCCEEHHHHHHHHHHCCCEEECCCCCCCCCCEEEEECCCEEEEECCCCCCCHHHHHHH
DACFWRLKSAKAVEFPQSTQKLTFTSGTTGHPKAVCLGSEQILTVAKSLSEAIDETKPQK
HHHHHHCCCCCEECCCCCCCEEEEECCCCCCCCEEEECCHHHHHHHHHHHHHHHHCCCHH
HLALMPLAVLLENIGCYAMLYRGAQIELVSASDLGLRGASGVDVHQFSHYLNQSKPHSLI
HHHHHHHHHHHHCCCEEEEEECCCEEEEEECCCCCCCCCCCCCHHHHHHHHCCCCCCEEE
LVPQFLSVLVEAVSSKLLRAEDYRFIAVGGGHVNPDLLKRAQTLNLPVYEGYGLSECASV
EHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHCCCCCCCCCCHHHHHHH
VALNTPAQQRAGSVGKPLAHVEVSIANDGEILVANNQCLGYLGSNHAPVKYWPTGDLGML
EEECCCHHHHCCCCCCCEEEEEEEEECCCEEEEECCCEEEEECCCCCCEEEECCCCCCCC
DEDGYLYVHGRKKHQFITSFGRNVNPEWVEALLSQTGVVGQAFVYGEALPENYALLWPVH
CCCCEEEEECCCHHHHHHHHCCCCCHHHHHHHHHHCCCCEEEEEECCCCCCCCEEEEECH
SAISNDQIEQVVEQANIKLPDYARVKHWVRLTEPFSTTNQLATANGRLRRDEIVSRYHNV
HHCCHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCCCCCCEECCCCCCCHHHHHHHHHHH
FSA
HCC
>Mature Secondary Structure
MLHKLSDFKSKLENYQFQQPYNIALSDKYQTINYADLLSEVNKRQEILKQLDVKVVALYL
CCCCHHHHHHHHHCCCCCCCCEEEECCCCCCCCHHHHHHHHHHHHHHHHHHCCEEEEEEE
ENSVDLMLWDLTLLFSEIPTIILPPFFSSSQLNYCLEISQADLVISNDNLNLDHLDLYFK
CCCCCEEHHHHHHHHHHCCCEEECCCCCCCCCCEEEEECCCEEEEECCCCCCCHHHHHHH
DACFWRLKSAKAVEFPQSTQKLTFTSGTTGHPKAVCLGSEQILTVAKSLSEAIDETKPQK
HHHHHHCCCCCEECCCCCCCEEEEECCCCCCCCEEEECCHHHHHHHHHHHHHHHHCCCHH
HLALMPLAVLLENIGCYAMLYRGAQIELVSASDLGLRGASGVDVHQFSHYLNQSKPHSLI
HHHHHHHHHHHHCCCEEEEEECCCEEEEEECCCCCCCCCCCCCHHHHHHHHCCCCCCEEE
LVPQFLSVLVEAVSSKLLRAEDYRFIAVGGGHVNPDLLKRAQTLNLPVYEGYGLSECASV
EHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHCCCCCCCCCCHHHHHHH
VALNTPAQQRAGSVGKPLAHVEVSIANDGEILVANNQCLGYLGSNHAPVKYWPTGDLGML
EEECCCHHHHCCCCCCCEEEEEEEEECCCEEEEECCCEEEEECCCCCCEEEECCCCCCCC
DEDGYLYVHGRKKHQFITSFGRNVNPEWVEALLSQTGVVGQAFVYGEALPENYALLWPVH
CCCCEEEEECCCHHHHHHHHCCCCCHHHHHHHHHHCCCCEEEEEECCCCCCCCEEEEECH
SAISNDQIEQVVEQANIKLPDYARVKHWVRLTEPFSTTNQLATANGRLRRDEIVSRYHNV
HHCCHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCCCCCCEECCCCCCCHHHHHHHHHHH
FSA
HCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 12663927 [H]