Click here to switch to the map view.

The map label for this gene is eno

Identifier: 50085136

GI number: 50085136

Start: 1991399

End: 1992694

Strand: Reverse

Name: eno

Synonym: ACIAD2001

Alternate gene names: 50085136

Gene position: 1992694-1991399 (Counterclockwise)

Preceding gene: 50085137

Following gene: 50085135

Centisome position: 55.37

GC content: 41.28

Gene sequence:

>1296_bases
TTGTTCATGAGCCAAATCGTTGACATTCGTGCACGTGAAATTTTGGACTCTCGTGGTAACCCGACCATCGAAGCAGACGT
AATCTTAGCATCTGGCGTAGTTGGCCGTGCATGTGCACCATCTGGTGCTTCAACTGGTTCTCGTGAAGCGTTAGAACTTC
GTGATGGCGACAAAGCACGCTATTTGGGTAAAGGTGTTAAAACTGCGGTTAACAATGTCAACACCATTATCCGCGATGCT
TTAGTGGGCAAATCAGTATTTGAACAAAAAGACATCGATAATACGATGATTGAGCTTGATGGTACTGAAAATAAAGAAAA
ATTAGGCGCAAATGCAACTTTAGCGGTATCGCTAGCTGCTGCACGCGCTGCTGCCGATGAAAAGAAAATTCCTCTTTTCC
AATACATCGCAGACTTACGTGGTCAAACCATTTTGACGATGCCTGTACCTATGATGAACATCATCAATGGTGGTTCGCAT
GCAGATAATAACGTTGATATTCAAGAATTTATGATTGAGCCTGTTGGCTTCACTTCATTCTCTGAAGCATTGCGTGCAGG
TGCTGAAATCTTCCATTCTCTAAAATCAGTTTTAAACAAAAAAGGTTTAAACACTGCTGTAGGTGATGAAGGTGGTTTTG
CGCCAAACTTGCGTTCTAACGAAGAGGCAATCACTGTTATTCTTGAAGCGATTGGTCAAACTGGCTATAAGGCGGGTTCT
GACATCATGCTTGCACTTGACTGTGCATCTTCAGAATTCTACAAAAATGGTCAGTACATCTTGGCAGGTGAAGGCAATAA
AGCATTCACAAGCAATCAGTTCTCTGACTATTTAGCGGGTCTTGTAAACCAGTATCCAATTATCTCAATTGAAGATGGTT
TAGATGAGTCTGATTGGGAAGGTTGGTCTTACTTGACATCTATTTTAGGTGACAAAATCCAGTTGGTGGGTGATGACTTG
TTCGTTACCAACCCGAAAATCCTTCAACGTGGTATCAATGAGAAAGTAGGTAACTCTATCTTAATCAAGTACAATCAAAT
TGGTACCTTGACTGAAACGCTTGATGCAATCTATCTTGCTAAAGACAATGGTTACTCTACAGTGATTTCACACCGTTCAG
GTGAAACTGAAGATTCAACCATCGCCGATCTTGCTGTGGGTACTGCTGCAGGTCAAATCAAAACAGGTTCACTTTGCCGT
TCTGATCGTGTAGCAAAATATAACCAATTACTTCGTATTGAAGAATTGACTAAAGCAGCTTATCGCGGTAAAGCTGAATT
CAAAGGCTTAAATTAA

Upstream 100 bases:

>100_bases
GGCACAATTAAAAGAATTGGATGCTTTAGTTAAAGGATTCAAAAAGTTAGATACTCATTGATGCAAATGATTGCATCTTA
TTTTTCATTCAATTGAGGAA

Downstream 100 bases:

>100_bases
TCTTTGGATACTATGTTAGAAGTCTTTAAATCAACTTCGAGCAAACTGATATTGGGGCTTGTGATTGTATTGGTCGCAGG
TCTTCAATATAGCTTTTGGT

Product: phosphopyruvate hydratase

Products: NA

Alternate protein names: 2-phospho-D-glycerate hydro-lyase; 2-phosphoglycerate dehydratase

Number of amino acids: Translated: 431; Mature: 431

Protein sequence:

>431_residues
MFMSQIVDIRAREILDSRGNPTIEADVILASGVVGRACAPSGASTGSREALELRDGDKARYLGKGVKTAVNNVNTIIRDA
LVGKSVFEQKDIDNTMIELDGTENKEKLGANATLAVSLAAARAAADEKKIPLFQYIADLRGQTILTMPVPMMNIINGGSH
ADNNVDIQEFMIEPVGFTSFSEALRAGAEIFHSLKSVLNKKGLNTAVGDEGGFAPNLRSNEEAITVILEAIGQTGYKAGS
DIMLALDCASSEFYKNGQYILAGEGNKAFTSNQFSDYLAGLVNQYPIISIEDGLDESDWEGWSYLTSILGDKIQLVGDDL
FVTNPKILQRGINEKVGNSILIKYNQIGTLTETLDAIYLAKDNGYSTVISHRSGETEDSTIADLAVGTAAGQIKTGSLCR
SDRVAKYNQLLRIEELTKAAYRGKAEFKGLN

Sequences:

>Translated_431_residues
MFMSQIVDIRAREILDSRGNPTIEADVILASGVVGRACAPSGASTGSREALELRDGDKARYLGKGVKTAVNNVNTIIRDA
LVGKSVFEQKDIDNTMIELDGTENKEKLGANATLAVSLAAARAAADEKKIPLFQYIADLRGQTILTMPVPMMNIINGGSH
ADNNVDIQEFMIEPVGFTSFSEALRAGAEIFHSLKSVLNKKGLNTAVGDEGGFAPNLRSNEEAITVILEAIGQTGYKAGS
DIMLALDCASSEFYKNGQYILAGEGNKAFTSNQFSDYLAGLVNQYPIISIEDGLDESDWEGWSYLTSILGDKIQLVGDDL
FVTNPKILQRGINEKVGNSILIKYNQIGTLTETLDAIYLAKDNGYSTVISHRSGETEDSTIADLAVGTAAGQIKTGSLCR
SDRVAKYNQLLRIEELTKAAYRGKAEFKGLN
>Mature_431_residues
MFMSQIVDIRAREILDSRGNPTIEADVILASGVVGRACAPSGASTGSREALELRDGDKARYLGKGVKTAVNNVNTIIRDA
LVGKSVFEQKDIDNTMIELDGTENKEKLGANATLAVSLAAARAAADEKKIPLFQYIADLRGQTILTMPVPMMNIINGGSH
ADNNVDIQEFMIEPVGFTSFSEALRAGAEIFHSLKSVLNKKGLNTAVGDEGGFAPNLRSNEEAITVILEAIGQTGYKAGS
DIMLALDCASSEFYKNGQYILAGEGNKAFTSNQFSDYLAGLVNQYPIISIEDGLDESDWEGWSYLTSILGDKIQLVGDDL
FVTNPKILQRGINEKVGNSILIKYNQIGTLTETLDAIYLAKDNGYSTVISHRSGETEDSTIADLAVGTAAGQIKTGSLCR
SDRVAKYNQLLRIEELTKAAYRGKAEFKGLN

Specific function: Catalyzes the reversible conversion of 2- phosphoglycerate into phosphoenolpyruvate. It is essential for the degradation of carbohydrates via glycolysis

COG id: COG0148

COG function: function code G; Enolase

Gene ontology:

Cell location: Cytoplasm. Secreted. Cell surface. Note=Fractions of enolase are present in both the cytoplasm and on the cell surface. The export of enolase possibly depends on the covalent binding to the substrate; once secreted, it remains attached to the bacterial ce

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enolase family

Homologues:

Organism=Homo sapiens, GI5803011, Length=418, Percent_Identity=54.5454545454545, Blast_Score=445, Evalue=1e-125,
Organism=Homo sapiens, GI4503571, Length=434, Percent_Identity=52.3041474654378, Blast_Score=440, Evalue=1e-123,
Organism=Homo sapiens, GI301897477, Length=426, Percent_Identity=53.0516431924883, Blast_Score=431, Evalue=1e-121,
Organism=Homo sapiens, GI301897469, Length=426, Percent_Identity=53.0516431924883, Blast_Score=431, Evalue=1e-121,
Organism=Homo sapiens, GI301897479, Length=424, Percent_Identity=47.877358490566, Blast_Score=373, Evalue=1e-103,
Organism=Homo sapiens, GI169201331, Length=340, Percent_Identity=27.9411764705882, Blast_Score=113, Evalue=3e-25,
Organism=Homo sapiens, GI169201757, Length=340, Percent_Identity=27.9411764705882, Blast_Score=113, Evalue=3e-25,
Organism=Homo sapiens, GI239744207, Length=340, Percent_Identity=27.9411764705882, Blast_Score=113, Evalue=3e-25,
Organism=Homo sapiens, GI310129182, Length=206, Percent_Identity=26.2135922330097, Blast_Score=68, Evalue=2e-11,
Organism=Homo sapiens, GI310110045, Length=206, Percent_Identity=26.2135922330097, Blast_Score=68, Evalue=2e-11,
Organism=Homo sapiens, GI310120572, Length=206, Percent_Identity=26.2135922330097, Blast_Score=68, Evalue=2e-11,
Organism=Escherichia coli, GI1789141, Length=430, Percent_Identity=68.8372093023256, Blast_Score=586, Evalue=1e-169,
Organism=Caenorhabditis elegans, GI71995829, Length=419, Percent_Identity=53.2219570405728, Blast_Score=435, Evalue=1e-122,
Organism=Caenorhabditis elegans, GI17536383, Length=419, Percent_Identity=53.2219570405728, Blast_Score=434, Evalue=1e-122,
Organism=Caenorhabditis elegans, GI32563855, Length=179, Percent_Identity=49.7206703910614, Blast_Score=188, Evalue=5e-48,
Organism=Saccharomyces cerevisiae, GI6321693, Length=421, Percent_Identity=50.1187648456057, Blast_Score=391, Evalue=1e-109,
Organism=Saccharomyces cerevisiae, GI6324974, Length=422, Percent_Identity=48.5781990521327, Blast_Score=382, Evalue=1e-107,
Organism=Saccharomyces cerevisiae, GI6324969, Length=422, Percent_Identity=48.5781990521327, Blast_Score=382, Evalue=1e-107,
Organism=Saccharomyces cerevisiae, GI6323985, Length=422, Percent_Identity=48.3412322274881, Blast_Score=380, Evalue=1e-106,
Organism=Saccharomyces cerevisiae, GI6321968, Length=432, Percent_Identity=48.6111111111111, Blast_Score=364, Evalue=1e-101,
Organism=Drosophila melanogaster, GI24580918, Length=416, Percent_Identity=54.5673076923077, Blast_Score=417, Evalue=1e-117,
Organism=Drosophila melanogaster, GI24580916, Length=416, Percent_Identity=54.5673076923077, Blast_Score=417, Evalue=1e-117,
Organism=Drosophila melanogaster, GI24580920, Length=416, Percent_Identity=54.5673076923077, Blast_Score=417, Evalue=1e-117,
Organism=Drosophila melanogaster, GI24580914, Length=416, Percent_Identity=54.5673076923077, Blast_Score=417, Evalue=1e-117,
Organism=Drosophila melanogaster, GI281360527, Length=416, Percent_Identity=54.5673076923077, Blast_Score=416, Evalue=1e-116,
Organism=Drosophila melanogaster, GI17137654, Length=416, Percent_Identity=54.5673076923077, Blast_Score=416, Evalue=1e-116,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1660 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 20 Molecules/Cell In: Stationary Phase,

Swissprot (AC and ID): ENO_ACIAD (Q6FAT9)

Other databases:

- EMBL:   CR543861
- RefSeq:   YP_046646.1
- ProteinModelPortal:   Q6FAT9
- SMR:   Q6FAT9
- STRING:   Q6FAT9
- GeneID:   2878443
- GenomeReviews:   CR543861_GR
- KEGG:   aci:ACIAD2001
- NMPDR:   fig|62977.3.peg.1908
- eggNOG:   COG0148
- HOGENOM:   HBG726599
- OMA:   DIAVGTN
- PhylomeDB:   Q6FAT9
- ProtClustDB:   PRK00077
- BioCyc:   ASP62977:ACIAD2001-MONOMER
- GO:   GO:0006096
- HAMAP:   MF_00318
- InterPro:   IPR000941
- InterPro:   IPR020810
- InterPro:   IPR020809
- InterPro:   IPR020811
- PIRSF:   PIRSF001400
- PRINTS:   PR00148
- TIGRFAMs:   TIGR01060

Pfam domain/function: PF00113 Enolase_C; PF03952 Enolase_N

EC number: =4.2.1.11

Molecular weight: Translated: 46413; Mature: 46413

Theoretical pI: Translated: 4.63; Mature: 4.63

Prosite motif: PS00164 ENOLASE

Important sites: ACT_SITE 210-210 ACT_SITE 343-343 BINDING 160-160 BINDING 169-169 BINDING 291-291 BINDING 318-318 BINDING 343-343 BINDING 394-394

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFMSQIVDIRAREILDSRGNPTIEADVILASGVVGRACAPSGASTGSREALELRDGDKAR
CCHHHHHHHHHHHHHHCCCCCCEEEEEEEECCCCCCCCCCCCCCCCCCCEEECCCCCHHH
YLGKGVKTAVNNVNTIIRDALVGKSVFEQKDIDNTMIELDGTENKEKLGANATLAVSLAA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCHHHHCCCCEEEEEHHH
ARAAADEKKIPLFQYIADLRGQTILTMPVPMMNIINGGSHADNNVDIQEFMIEPVGFTSF
HHHHCCCCCCHHHHHHHHHCCCEEEEECCCHHHHHCCCCCCCCCCCHHHHHCCCCCCCHH
SEALRAGAEIFHSLKSVLNKKGLNTAVGDEGGFAPNLRSNEEAITVILEAIGQTGYKAGS
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCC
DIMLALDCASSEFYKNGQYILAGEGNKAFTSNQFSDYLAGLVNQYPIISIEDGLDESDWE
CEEEEEECCCHHHHCCCCEEEEECCCCCCCCCHHHHHHHHHHHCCCEEEECCCCCCCCCC
GWSYLTSILGDKIQLVGDDLFVTNPKILQRGINEKVGNSILIKYNQIGTLTETLDAIYLA
HHHHHHHHHCCCEEEECCCEEECCHHHHHHCCHHHHCCEEEEEECCCCCHHHHHHEEEEE
KDNGYSTVISHRSGETEDSTIADLAVGTAAGQIKTGSLCRSDRVAKYNQLLRIEELTKAA
CCCCCEEEEECCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
YRGKAEFKGLN
HCCCCCCCCCC
>Mature Secondary Structure
MFMSQIVDIRAREILDSRGNPTIEADVILASGVVGRACAPSGASTGSREALELRDGDKAR
CCHHHHHHHHHHHHHHCCCCCCEEEEEEEECCCCCCCCCCCCCCCCCCCEEECCCCCHHH
YLGKGVKTAVNNVNTIIRDALVGKSVFEQKDIDNTMIELDGTENKEKLGANATLAVSLAA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCHHHHCCCCEEEEEHHH
ARAAADEKKIPLFQYIADLRGQTILTMPVPMMNIINGGSHADNNVDIQEFMIEPVGFTSF
HHHHCCCCCCHHHHHHHHHCCCEEEEECCCHHHHHCCCCCCCCCCCHHHHHCCCCCCCHH
SEALRAGAEIFHSLKSVLNKKGLNTAVGDEGGFAPNLRSNEEAITVILEAIGQTGYKAGS
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCC
DIMLALDCASSEFYKNGQYILAGEGNKAFTSNQFSDYLAGLVNQYPIISIEDGLDESDWE
CEEEEEECCCHHHHCCCCEEEEECCCCCCCCCHHHHHHHHHHHCCCEEEECCCCCCCCCC
GWSYLTSILGDKIQLVGDDLFVTNPKILQRGINEKVGNSILIKYNQIGTLTETLDAIYLA
HHHHHHHHHCCCEEEECCCEEECCHHHHHHCCHHHHCCEEEEEECCCCCHHHHHHEEEEE
KDNGYSTVISHRSGETEDSTIADLAVGTAAGQIKTGSLCRSDRVAKYNQLLRIEELTKAA
CCCCCEEEEECCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
YRGKAEFKGLN
HCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA