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The map label for this gene is pgk

Identifier: 50085067

GI number: 50085067

Start: 1916850

End: 1918037

Strand: Reverse

Name: pgk

Synonym: ACIAD1927

Alternate gene names: 50085067

Gene position: 1918037-1916850 (Counterclockwise)

Preceding gene: 50085071

Following gene: 50085066

Centisome position: 53.3

GC content: 43.27

Gene sequence:

>1188_bases
ATGAACTTTAAGCGTATGACGGACCTCGACTTAAGCGAAAAACGTGTATTAATTCGTGAAGATTTAAATGTACCAGTAAA
AAATGGCCAGATTACCAGTGATGCTCGTTTGCGTGCAGCATTACCAACCATTCAGGCTGCACTTGCTCAAGGTGCTGCTG
TGATGGTTTGCTCTCACTTAGGGCGTCCTGTTGAAGGTGAACCAAAACCTGAACAGTCACTTGCACCTGTTGCAGCTTAC
CTTTCAGATGCATTAGGGCAGGAAGTTAAACTTTTGACTGACTACCTTGAAGGGGTTGAGATAGCACCAGGTCAAGTTGT
TCTTCTTGAAAATGTACGTTTTAACCTCGGCGAAAAGAAAAATAATGCTGAACTTGCACAAAAATATGCAGCACTCTGTG
ATGTATTCGTGATGGATGCATTTGGCACTGCACATCGTGCAGAAGCATCAACCGAAGGTGCTGCTCGTTATGCCAAAGTT
GCAGCGGCAGGCCCATTGCTTGCGACTGAGCTAGATGCATTAGGTCGTGCACTTCAAACCCCTGAAAAGCCAATGGTGGC
GATTGTTGCAGGTTCTAAAGTTTCAACAAAACTTGATGTTTTAACCTCACTTTCTGACATTTGCGGTCAATTGATTGTGG
GTGGTGGAATTGCCAATACCTTCTTGGCAGCAGCTGGTTTCAATGTAGGCAAATCGCTTTGTGAAAATGATTTGATTGAT
ACCGCTAAAGCAATTGCCGCTAAAGTATCTGTTCCACTTCCTACGGATGTGGTGGTAGCTGATGCCACAGAGATTGACTT
TGCTGACTTCTTGGGTTCATTAGCAAAAGCACAAGCAATTGTGAAAAAAGTGGAAGATATTGCTGACAATGACATGATTC
TGGATGTGGGTCCTGACACGGCGAAAGCATTTGCTGAGATCTTAAAAACAGCTAAAACGATACTTTGGAACGGCCCAGTT
GGTGTATTTGAAGTAGATCAGTTTGGTGAAGGAACCAAAACGCTTTCATTGGCAATTGCTGAATCTAAAGGATTTTCTAT
TGCAGGTGGTGGTGATACTTTGGCTGCAATTGATAAATATAAAGTTGCTGACAAAATTGGTTATATCTCAACAGGTGGCG
GCGCATTCCTTGAGTTTGTAGAAGGAAAAACCTTACCAGCCGTTGCTGTATTACTCGAACGTGCCTAA

Upstream 100 bases:

>100_bases
ATCTTTCGGCAGCTTTTCTACAAAAAAACTCTCTTTTTTTATCATAATCATTTAAAATATCGCCATTTATAATTTTCATA
AGAGTAAAAGAGAGCACTCA

Downstream 100 bases:

>100_bases
TCGCTGACGTGTTTTTAAAAGAACCGGCCTTATGGTCGGTTCTTTCATAAGAGATTCATCGAACTGTCACATATCTGCAA
TAAAATATTTTTCAGTTAAA

Product: phosphoglycerate kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 395; Mature: 395

Protein sequence:

>395_residues
MNFKRMTDLDLSEKRVLIREDLNVPVKNGQITSDARLRAALPTIQAALAQGAAVMVCSHLGRPVEGEPKPEQSLAPVAAY
LSDALGQEVKLLTDYLEGVEIAPGQVVLLENVRFNLGEKKNNAELAQKYAALCDVFVMDAFGTAHRAEASTEGAARYAKV
AAAGPLLATELDALGRALQTPEKPMVAIVAGSKVSTKLDVLTSLSDICGQLIVGGGIANTFLAAAGFNVGKSLCENDLID
TAKAIAAKVSVPLPTDVVVADATEIDFADFLGSLAKAQAIVKKVEDIADNDMILDVGPDTAKAFAEILKTAKTILWNGPV
GVFEVDQFGEGTKTLSLAIAESKGFSIAGGGDTLAAIDKYKVADKIGYISTGGGAFLEFVEGKTLPAVAVLLERA

Sequences:

>Translated_395_residues
MNFKRMTDLDLSEKRVLIREDLNVPVKNGQITSDARLRAALPTIQAALAQGAAVMVCSHLGRPVEGEPKPEQSLAPVAAY
LSDALGQEVKLLTDYLEGVEIAPGQVVLLENVRFNLGEKKNNAELAQKYAALCDVFVMDAFGTAHRAEASTEGAARYAKV
AAAGPLLATELDALGRALQTPEKPMVAIVAGSKVSTKLDVLTSLSDICGQLIVGGGIANTFLAAAGFNVGKSLCENDLID
TAKAIAAKVSVPLPTDVVVADATEIDFADFLGSLAKAQAIVKKVEDIADNDMILDVGPDTAKAFAEILKTAKTILWNGPV
GVFEVDQFGEGTKTLSLAIAESKGFSIAGGGDTLAAIDKYKVADKIGYISTGGGAFLEFVEGKTLPAVAVLLERA
>Mature_395_residues
MNFKRMTDLDLSEKRVLIREDLNVPVKNGQITSDARLRAALPTIQAALAQGAAVMVCSHLGRPVEGEPKPEQSLAPVAAY
LSDALGQEVKLLTDYLEGVEIAPGQVVLLENVRFNLGEKKNNAELAQKYAALCDVFVMDAFGTAHRAEASTEGAARYAKV
AAAGPLLATELDALGRALQTPEKPMVAIVAGSKVSTKLDVLTSLSDICGQLIVGGGIANTFLAAAGFNVGKSLCENDLID
TAKAIAAKVSVPLPTDVVVADATEIDFADFLGSLAKAQAIVKKVEDIADNDMILDVGPDTAKAFAEILKTAKTILWNGPV
GVFEVDQFGEGTKTLSLAIAESKGFSIAGGGDTLAAIDKYKVADKIGYISTGGGAFLEFVEGKTLPAVAVLLERA

Specific function: Second phase of glycolysis; second step. [C]

COG id: COG0126

COG function: function code G; 3-phosphoglycerate kinase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphoglycerate kinase family

Homologues:

Organism=Homo sapiens, GI4505763, Length=416, Percent_Identity=40.8653846153846, Blast_Score=257, Evalue=1e-68,
Organism=Homo sapiens, GI31543397, Length=423, Percent_Identity=39.9527186761229, Blast_Score=257, Evalue=2e-68,
Organism=Escherichia coli, GI1789294, Length=395, Percent_Identity=64.0506329113924, Blast_Score=491, Evalue=1e-140,
Organism=Caenorhabditis elegans, GI17508823, Length=425, Percent_Identity=41.4117647058824, Blast_Score=277, Evalue=6e-75,
Organism=Saccharomyces cerevisiae, GI6319857, Length=421, Percent_Identity=41.5676959619952, Blast_Score=258, Evalue=1e-69,
Organism=Drosophila melanogaster, GI17136394, Length=427, Percent_Identity=40.7494145199063, Blast_Score=253, Evalue=2e-67,
Organism=Drosophila melanogaster, GI24581203, Length=419, Percent_Identity=38.1861575178998, Blast_Score=233, Evalue=2e-61,

Paralogues:

None

Copy number: 2990 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1460 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). [C]

Swissprot (AC and ID): PGK_ACIAD (Q6FB08)

Other databases:

- EMBL:   CR543861
- RefSeq:   YP_046577.1
- HSSP:   P36204
- ProteinModelPortal:   Q6FB08
- SMR:   Q6FB08
- STRING:   Q6FB08
- GeneID:   2879593
- GenomeReviews:   CR543861_GR
- KEGG:   aci:ACIAD1927
- NMPDR:   fig|62977.3.peg.1839
- eggNOG:   COG0126
- HOGENOM:   HBG453500
- OMA:   NCAIILP
- PhylomeDB:   Q6FB08
- ProtClustDB:   PRK00073
- BioCyc:   ASP62977:ACIAD1927-MONOMER
- GO:   GO:0005737
- GO:   GO:0006096
- HAMAP:   MF_00145
- InterPro:   IPR001576
- InterPro:   IPR015901
- InterPro:   IPR015911
- InterPro:   IPR015824
- Gene3D:   G3DSA:3.40.50.1270
- Gene3D:   G3DSA:3.40.50.1260
- PANTHER:   PTHR11406
- PIRSF:   PIRSF000724
- PRINTS:   PR00477

Pfam domain/function: PF00162 PGK; SSF53748 PGK

EC number: =2.7.2.3

Molecular weight: Translated: 41257; Mature: 41257

Theoretical pI: Translated: 4.57; Mature: 4.57

Prosite motif: PS00111 PGLYCERATE_KINASE

Important sites: BINDING 36-36 BINDING 113-113 BINDING 146-146 BINDING 197-197 BINDING 324-324

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNFKRMTDLDLSEKRVLIREDLNVPVKNGQITSDARLRAALPTIQAALAQGAAVMVCSHL
CCCCCCCCCCCCCCEEEEECCCCCCCCCCEECCCCHHHHHHHHHHHHHHCCHHHHHHHHC
GRPVEGEPKPEQSLAPVAAYLSDALGQEVKLLTDYLEGVEIAPGQVVLLENVRFNLGEKK
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCEECCCCEEEEECCCCCCCCCC
NNAELAQKYAALCDVFVMDAFGTAHRAEASTEGAARYAKVAAAGPLLATELDALGRALQT
CHHHHHHHHHHHHHHHHHHHCCCHHHCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHCC
PEKPMVAIVAGSKVSTKLDVLTSLSDICGQLIVGGGIANTFLAAAGFNVGKSLCENDLID
CCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCHHHHHHHHHHHHH
TAKAIAAKVSVPLPTDVVVADATEIDFADFLGSLAKAQAIVKKVEDIADNDMILDVGPDT
HHHHHHHHCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCH
AKAFAEILKTAKTILWNGPVGVFEVDQFGEGTKTLSLAIAESKGFSIAGGGDTLAAIDKY
HHHHHHHHHHHHHEEECCCCCEEEECCCCCCCCEEEEEEECCCCCEEECCCCCHHHHHHH
KVADKIGYISTGGGAFLEFVEGKTLPAVAVLLERA
HHHHHCCEEECCCHHHHHHHCCCCHHHHHHHHHCC
>Mature Secondary Structure
MNFKRMTDLDLSEKRVLIREDLNVPVKNGQITSDARLRAALPTIQAALAQGAAVMVCSHL
CCCCCCCCCCCCCCEEEEECCCCCCCCCCEECCCCHHHHHHHHHHHHHHCCHHHHHHHHC
GRPVEGEPKPEQSLAPVAAYLSDALGQEVKLLTDYLEGVEIAPGQVVLLENVRFNLGEKK
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCEECCCCEEEEECCCCCCCCCC
NNAELAQKYAALCDVFVMDAFGTAHRAEASTEGAARYAKVAAAGPLLATELDALGRALQT
CHHHHHHHHHHHHHHHHHHHCCCHHHCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHCC
PEKPMVAIVAGSKVSTKLDVLTSLSDICGQLIVGGGIANTFLAAAGFNVGKSLCENDLID
CCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCHHHHHHHHHHHHH
TAKAIAAKVSVPLPTDVVVADATEIDFADFLGSLAKAQAIVKKVEDIADNDMILDVGPDT
HHHHHHHHCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCH
AKAFAEILKTAKTILWNGPVGVFEVDQFGEGTKTLSLAIAESKGFSIAGGGDTLAAIDKY
HHHHHHHHHHHHHEEECCCCCEEEECCCCCCCCEEEEEEECCCCCEEECCCCCHHHHHHH
KVADKIGYISTGGGAFLEFVEGKTLPAVAVLLERA
HHHHHCCEEECCCHHHHHHHCCCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA