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The map label for this gene is secDF

Identifier: 49481498

GI number: 49481498

Start: 4207769

End: 4210033

Strand: Reverse

Name: secDF

Synonym: BT9727_4144

Alternate gene names: 49481498

Gene position: 4210033-4207769 (Counterclockwise)

Preceding gene: 49480666

Following gene: 49481497

Centisome position: 80.38

GC content: 37.04

Gene sequence:

>2265_bases
ATGGCAAAGCGTGGTACGAGAATTGCCGCCTTTTTCCTCATCGTTTTATTAATCGGTGGAGTAATTGGTGCGGCAGGAAA
AGACATAGCAAAAGGAATTAGTCTAGGACTAGACCTTCGCGGTGGTTTTGAAATTCTGTATGAAGTAAAACCAGCCAAAA
AAGGTGATAAAATCGATCGAGACGTACTTGTAAGTACAGTAGGTGCTCTTGAAAATCGTGTCAATGTTCTCGGTGTAAGT
GAGCCGAACATTCAAATCGAAGGACAAGATCGAATTCGTGTACAGCTTGCTGGTGTACAAGATCAGCAAAAAGCTCGTGA
AATGTTGTCAACACAAGCGAAATTAACATTCCGTGATGTAGATGACAATCTTCTTATGGATGGAACGGATTTAAAAGGTG
GCGGAGCGAAGCAAACGTTTGATGAGCAAGGCCGTGCGAGTGTAGGTCTTACACTAAAAAGCGCTGAAAAATTCCGTGAA
GTAACTGAAAAAATTTCAAAAATGCCACCACCAACAAATTTAATGGTAATTTGGCTTGATTTTGAAGAAGGAAAAGATTC
GTATAAAGCAGAATCTGCAAAACCGAATCCGAAATTCTTATCAGCAGCAACAGTAAACCAAGTGTTTAACCAAGCTGAAG
TATCTATCGTAGGTGGAAACTTCACAGTTGAAAGTGCGAAACAACTTTCATCTTTATTAAATGCAGGTGCACTTCCGGTT
GATTTAAAAGAAATGTATTCAACATCAGTTGGAGCGAAGTTTGGTCAACAAGCGTTAGAGCAAACAATTTTCGCTAGTGC
GATTGGTATCGCTCTTATTTTCTTATTTATGCTTGTATTCTACCGTTTACCAGGAATTGTAGCGGTTATTATGTTAGGTT
TATACATTTTCGTTACATTACTTGTCTTTAACTGGATGCATGCAGTTCTGACGTTGCCAGGTATTGCGGCGTTAGTGCTC
GGGGTAGGTATCGCAGTTGATGCGAATATTATTACGTACGAGAGACTAAAAGAAGAGCTGAAAATTGGTAAGTCAATGAT
GTCAGCATTCCGTGCTGGTAACCATCGTTCATTAGCAACAATTTTAGATGCGAACATTACAACACTTGCAGCAGCTGGTG
TATTATTCGTTTACGGTAATAGCTCTGTAAAAGGATTCGCAACAAGTTTAATTGTAAGTATTTTAGTTGGTTTTATTACG
AACGTATTCGGTACACGTTTCTTACTAAGTTTACTTGTAAAGAGCCGTTACTTCGATAAAAAACCAGGATACTTTGGTGT
GAAACAAAAGGATATTATTCCGTTAACAAAAGGTGTAGTACATCCACCAACGAAATTTGATCGTATTAACTTTGTAAATG
TCGGTCATAAATTCCTTATCTTCTCGATTGTAGTTGTAATTGCAGGTGCAATTATTTTACCAATTTTCAAAATGAATCTT
GGTATTGACTTTGCGAGTGGTACACGTATCGACTTGCAATCAAAACAATCAGTTACTGTGTCTGACGTTCATAAAGATTT
TAAAGAATTGAACATTGATGTGAAAGAAGAAAATATTGTACCGACTGGAGATGACAATAAAGGATTCGCAGTTCGTACAT
TAGGTGTTCTATCAAAAGATGAAATTGCGAAAACAAAAACATTCTTCCACGATAAATATGGTACAGATCCAAACGTAAGT
ACAGTTTCACCGACAATCGGTAAAGAAATTGCACGAAATGCATTTATCGCAGTATTAATTGCTTCTGCAGTTATCATTTT
ATACGTAAGTATTCGATTCCGATTTACGTACGCATTATCTGCAGTACTTGCATTACTACATGATGCATTCGTTATGATTG
TTATATTTAGTATTTTACAATTAGAGGTAGACTTAACGTTTATCGCTGCGGTATTAACAATCATCGGTTATTCTATTAAT
GACTCGATTGTTACGTTTGATAGAAACCGTGAATTATACAAACAGAAAAAACGAGTTCGTGATATAAAAGACTTAGAAGA
AATCGTAAATGCAAGTATTCGCCAAACGCTTGGTCGTTCGATTAACACCGTATTAACAGTGTTATTCCCAGTAATTGCAC
TGCTTATCTTCGGTAGTGAGTCACTACGTAACTTCTCGTTTGCGTTACTAGTTGGATTAATTGTAGGTACGTATTCTTCT
GTTTTCGTTGCCTCTCAAATTTGGTTAATGCTTGAAAACCGTCGTTTGAAAAAGGGTAAAAACAAGAAGAAGGTTGAGAA
AGTAGAATCTGAACCGCAAGTATAA

Upstream 100 bases:

>100_bases
AAATTATAGAAAGGAAGTCTACTAACTAGGCGTATGCACACGTGTAAAAGTGAATCGTAAAAGATTTATTTGAAGTGTTA
GTAGCGGAAAGAGGGTACAT

Downstream 100 bases:

>100_bases
AAAAGATGATGTCTCTTTAGGAGGCATCTCTTTTTTCGTTTGTGGATACAATAAGATAGCAACTTTGAAATGATAAAATG
GAAGAAAGTGGTGATGTTAT

Product: bifunctional preprotein translocase subunit SecD/SecF

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 754; Mature: 753

Protein sequence:

>754_residues
MAKRGTRIAAFFLIVLLIGGVIGAAGKDIAKGISLGLDLRGGFEILYEVKPAKKGDKIDRDVLVSTVGALENRVNVLGVS
EPNIQIEGQDRIRVQLAGVQDQQKAREMLSTQAKLTFRDVDDNLLMDGTDLKGGGAKQTFDEQGRASVGLTLKSAEKFRE
VTEKISKMPPPTNLMVIWLDFEEGKDSYKAESAKPNPKFLSAATVNQVFNQAEVSIVGGNFTVESAKQLSSLLNAGALPV
DLKEMYSTSVGAKFGQQALEQTIFASAIGIALIFLFMLVFYRLPGIVAVIMLGLYIFVTLLVFNWMHAVLTLPGIAALVL
GVGIAVDANIITYERLKEELKIGKSMMSAFRAGNHRSLATILDANITTLAAAGVLFVYGNSSVKGFATSLIVSILVGFIT
NVFGTRFLLSLLVKSRYFDKKPGYFGVKQKDIIPLTKGVVHPPTKFDRINFVNVGHKFLIFSIVVVIAGAIILPIFKMNL
GIDFASGTRIDLQSKQSVTVSDVHKDFKELNIDVKEENIVPTGDDNKGFAVRTLGVLSKDEIAKTKTFFHDKYGTDPNVS
TVSPTIGKEIARNAFIAVLIASAVIILYVSIRFRFTYALSAVLALLHDAFVMIVIFSILQLEVDLTFIAAVLTIIGYSIN
DSIVTFDRNRELYKQKKRVRDIKDLEEIVNASIRQTLGRSINTVLTVLFPVIALLIFGSESLRNFSFALLVGLIVGTYSS
VFVASQIWLMLENRRLKKGKNKKKVEKVESEPQV

Sequences:

>Translated_754_residues
MAKRGTRIAAFFLIVLLIGGVIGAAGKDIAKGISLGLDLRGGFEILYEVKPAKKGDKIDRDVLVSTVGALENRVNVLGVS
EPNIQIEGQDRIRVQLAGVQDQQKAREMLSTQAKLTFRDVDDNLLMDGTDLKGGGAKQTFDEQGRASVGLTLKSAEKFRE
VTEKISKMPPPTNLMVIWLDFEEGKDSYKAESAKPNPKFLSAATVNQVFNQAEVSIVGGNFTVESAKQLSSLLNAGALPV
DLKEMYSTSVGAKFGQQALEQTIFASAIGIALIFLFMLVFYRLPGIVAVIMLGLYIFVTLLVFNWMHAVLTLPGIAALVL
GVGIAVDANIITYERLKEELKIGKSMMSAFRAGNHRSLATILDANITTLAAAGVLFVYGNSSVKGFATSLIVSILVGFIT
NVFGTRFLLSLLVKSRYFDKKPGYFGVKQKDIIPLTKGVVHPPTKFDRINFVNVGHKFLIFSIVVVIAGAIILPIFKMNL
GIDFASGTRIDLQSKQSVTVSDVHKDFKELNIDVKEENIVPTGDDNKGFAVRTLGVLSKDEIAKTKTFFHDKYGTDPNVS
TVSPTIGKEIARNAFIAVLIASAVIILYVSIRFRFTYALSAVLALLHDAFVMIVIFSILQLEVDLTFIAAVLTIIGYSIN
DSIVTFDRNRELYKQKKRVRDIKDLEEIVNASIRQTLGRSINTVLTVLFPVIALLIFGSESLRNFSFALLVGLIVGTYSS
VFVASQIWLMLENRRLKKGKNKKKVEKVESEPQV
>Mature_753_residues
AKRGTRIAAFFLIVLLIGGVIGAAGKDIAKGISLGLDLRGGFEILYEVKPAKKGDKIDRDVLVSTVGALENRVNVLGVSE
PNIQIEGQDRIRVQLAGVQDQQKAREMLSTQAKLTFRDVDDNLLMDGTDLKGGGAKQTFDEQGRASVGLTLKSAEKFREV
TEKISKMPPPTNLMVIWLDFEEGKDSYKAESAKPNPKFLSAATVNQVFNQAEVSIVGGNFTVESAKQLSSLLNAGALPVD
LKEMYSTSVGAKFGQQALEQTIFASAIGIALIFLFMLVFYRLPGIVAVIMLGLYIFVTLLVFNWMHAVLTLPGIAALVLG
VGIAVDANIITYERLKEELKIGKSMMSAFRAGNHRSLATILDANITTLAAAGVLFVYGNSSVKGFATSLIVSILVGFITN
VFGTRFLLSLLVKSRYFDKKPGYFGVKQKDIIPLTKGVVHPPTKFDRINFVNVGHKFLIFSIVVVIAGAIILPIFKMNLG
IDFASGTRIDLQSKQSVTVSDVHKDFKELNIDVKEENIVPTGDDNKGFAVRTLGVLSKDEIAKTKTFFHDKYGTDPNVST
VSPTIGKEIARNAFIAVLIASAVIILYVSIRFRFTYALSAVLALLHDAFVMIVIFSILQLEVDLTFIAAVLTIIGYSIND
SIVTFDRNRELYKQKKRVRDIKDLEEIVNASIRQTLGRSINTVLTVLFPVIALLIFGSESLRNFSFALLVGLIVGTYSSV
FVASQIWLMLENRRLKKGKNKKKVEKVESEPQV

Specific function: Required for efficient translocation of secretory pre- proteins under conditions of hypersecretion but is not required for the release of mature proteins from the membrane [H]

COG id: COG0342

COG function: function code U; Preprotein translocase subunit SecD

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the secD/secF family. SecF subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786609, Length=379, Percent_Identity=31.1345646437995, Blast_Score=157, Evalue=2e-39,
Organism=Escherichia coli, GI1786610, Length=291, Percent_Identity=31.6151202749141, Blast_Score=137, Evalue=2e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005791
- InterPro:   IPR022813
- InterPro:   IPR022646
- InterPro:   IPR022645
- InterPro:   IPR005665 [H]

Pfam domain/function: PF07549 Sec_GG; PF02355 SecD_SecF [H]

EC number: NA

Molecular weight: Translated: 82611; Mature: 82480

Theoretical pI: Translated: 10.07; Mature: 10.07

Prosite motif: PS00501 SPASE_I_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKRGTRIAAFFLIVLLIGGVIGAAGKDIAKGISLGLDLRGGFEILYEVKPAKKGDKIDR
CCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCCEEEEEEECCCCCCCCCHH
DVLVSTVGALENRVNVLGVSEPNIQIEGQDRIRVQLAGVQDQQKAREMLSTQAKLTFRDV
HHHHHHHHHHHCCCEEEECCCCCEEECCCCEEEEEEECCCHHHHHHHHHHHHHHEEEEEC
DDNLLMDGTDLKGGGAKQTFDEQGRASVGLTLKSAEKFREVTEKISKMPPPTNLMVIWLD
CCCEEECCCCCCCCCCCCCHHHCCCCEEEEEHHHHHHHHHHHHHHHCCCCCCCEEEEEEE
FEEGKDSYKAESAKPNPKFLSAATVNQVFNQAEVSIVGGNFTVESAKQLSSLLNAGALPV
ECCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCEEEEECCCEEHHHHHHHHHHHCCCCCCC
DLKEMYSTSVGAKFGQQALEQTIFASAIGIALIFLFMLVFYRLPGIVAVIMLGLYIFVTL
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LVFNWMHAVLTLPGIAALVLGVGIAVDANIITYERLKEELKIGKSMMSAFRAGNHRSLAT
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHH
ILDANITTLAAAGVLFVYGNSSVKGFATSLIVSILVGFITNVFGTRFLLSLLVKSRYFDK
HHHCCHHHHHHCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
KPGYFGVKQKDIIPLTKGVVHPPTKFDRINFVNVGHKFLIFSIVVVIAGAIILPIFKMNL
CCCCCCCCCCCCCCHHCCCCCCCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHC
GIDFASGTRIDLQSKQSVTVSDVHKDFKELNIDVKEENIVPTGDDNKGFAVRTLGVLSKD
CEEECCCCEEEECCCCCCHHHHHHHHHHHCCCCEECCCCCCCCCCCCCEEEEEECCCCHH
EIAKTKTFFHDKYGTDPNVSTVSPTIGKEIARNAFIAVLIASAVIILYVSIRFRFTYALS
HHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AVLALLHDAFVMIVIFSILQLEVDLTFIAAVLTIIGYSINDSIVTFDRNRELYKQKKRVR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCHHHHHHHHHHH
DIKDLEEIVNASIRQTLGRSINTVLTVLFPVIALLIFGSESLRNFSFALLVGLIVGTYSS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHH
VFVASQIWLMLENRRLKKGKNKKKVEKVESEPQV
HHHHHHHHHHHHCCHHHCCCCHHHHHHHHCCCCC
>Mature Secondary Structure 
AKRGTRIAAFFLIVLLIGGVIGAAGKDIAKGISLGLDLRGGFEILYEVKPAKKGDKIDR
CCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCCEEEEEEECCCCCCCCCHH
DVLVSTVGALENRVNVLGVSEPNIQIEGQDRIRVQLAGVQDQQKAREMLSTQAKLTFRDV
HHHHHHHHHHHCCCEEEECCCCCEEECCCCEEEEEEECCCHHHHHHHHHHHHHHEEEEEC
DDNLLMDGTDLKGGGAKQTFDEQGRASVGLTLKSAEKFREVTEKISKMPPPTNLMVIWLD
CCCEEECCCCCCCCCCCCCHHHCCCCEEEEEHHHHHHHHHHHHHHHCCCCCCCEEEEEEE
FEEGKDSYKAESAKPNPKFLSAATVNQVFNQAEVSIVGGNFTVESAKQLSSLLNAGALPV
ECCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCEEEEECCCEEHHHHHHHHHHHCCCCCCC
DLKEMYSTSVGAKFGQQALEQTIFASAIGIALIFLFMLVFYRLPGIVAVIMLGLYIFVTL
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LVFNWMHAVLTLPGIAALVLGVGIAVDANIITYERLKEELKIGKSMMSAFRAGNHRSLAT
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHH
ILDANITTLAAAGVLFVYGNSSVKGFATSLIVSILVGFITNVFGTRFLLSLLVKSRYFDK
HHHCCHHHHHHCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
KPGYFGVKQKDIIPLTKGVVHPPTKFDRINFVNVGHKFLIFSIVVVIAGAIILPIFKMNL
CCCCCCCCCCCCCCHHCCCCCCCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHC
GIDFASGTRIDLQSKQSVTVSDVHKDFKELNIDVKEENIVPTGDDNKGFAVRTLGVLSKD
CEEECCCCEEEECCCCCCHHHHHHHHHHHCCCCEECCCCCCCCCCCCCEEEEEECCCCHH
EIAKTKTFFHDKYGTDPNVSTVSPTIGKEIARNAFIAVLIASAVIILYVSIRFRFTYALS
HHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AVLALLHDAFVMIVIFSILQLEVDLTFIAAVLTIIGYSINDSIVTFDRNRELYKQKKRVR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCHHHHHHHHHHH
DIKDLEEIVNASIRQTLGRSINTVLTVLFPVIALLIFGSESLRNFSFALLVGLIVGTYSS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHH
VFVASQIWLMLENRRLKKGKNKKKVEKVESEPQV
HHHHHHHHHHHHCCHHHCCCCHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9694879; 9384377 [H]