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The map label for this gene is nos [H]

Identifier: 49188283

GI number: 49188283

Start: 5186015

End: 5187085

Strand: Reverse

Name: nos [H]

Synonym: BAS5299

Alternate gene names: 49188283

Gene position: 5187085-5186015 (Counterclockwise)

Preceding gene: 49188285

Following gene: 49188282

Centisome position: 99.2

GC content: 37.54

Gene sequence:

>1071_bases
ATGAGTAAAACGAAACAATTAATAGAGGAAGCGAGTCATTTTATTACGATTTGCTATAAAGAGCTTAGTAAAGAACATTT
CATAGAAGAACGCATGAAAGAAATTCAAGCTGAGATAGAGAAGACAGGGACATATGAGCATACATTTGAAGAACTTGTTC
ATGGATCGCGAATGGCATGGCGCAATAGTAATCGATGTATCGGAAGACTATTTTGGAGTAAGATGCACATATTAGATGCA
CGTGAAGTAAACGATGAGGAAGGTGTATATCATGCATTAATTCATCATATTAAATATGCAACGAACGACGGAAAAGTGAA
ACCGACAATTACAATTTTTAAGCAATATCAAGGTGAAGAAAATAATATACGAATTTATAATCATCAATTAATTCGATATG
CAGGATATAAAACAGAAATGGGAGTGACTGGTGACTCTCATTCCACTGCATTTACAGATTTTTGTCAGGAACTTGGCTGG
CAAGGAGAAGGCACGAATTTTGATGTATTGCCACTTGTGTTTTCTATAGATGGAAAAGCGCCTATATATAAAGAAATTCC
GAAAGAAGAAGTAAAAGAAGTGCCAATTGAACACCCAGAATACCCGATTTCATCACTTGGAGCAAAATGGTACGGGGTTC
CAATGATTTCAGATATGCGCTTAGAAATTGGTGGTATTTCCTATACAGCAGCTCCGTTTAATGGATGGTACATGGGTACG
GAAATTGGGGCGCGTAACTTAGCGGATCATGATCGTTATAATTTACTGCCCGCCGTTGCGGAGATGATGGATCTAGATAC
ATCGAGAAACGGTACATTATGGAAAGACAAGGCATTAATTGAATTAAACGTGGCTGTTTTACATTCCTTTAAAAAACAAG
GAGTTAGTATTGTTGATCATCATACTGCTGCACAACAATTTCAGCAATTTGAGAAGCAAGAAGCTGCTTGTGGTCGTGTT
GTAACCGGCAATTGGGTGTGGCTCATTCCACCGTTATCTCCAGCTACCACTCATATTTATCATAAACCGTATCCAAATGA
AATTTTGAAGCCGAATTTCTTTCATAAATAG

Upstream 100 bases:

>100_bases
TTACAATAAAAGTAACACTGCGACTTTCATTTGTGACTCTCGTTCTTTTTGCTTATACTGTAAAGTAGTAATGTTTTGTT
GAAATAGAAGGAGATCGTTT

Downstream 100 bases:

>100_bases
TTTGTAGTATAAGGCATTTTCTATAAAAATATAATTTTTTTAATTACTGTTACGTAGCAAATGGAAGCCTTTACTCTCGG
TTATTTTTGGAAGGAGCTTT

Product: nitric-oxide synthase, oxygenase subunit

Products: citrulline; nitric oxide; NADP+

Alternate protein names: NOSoxy-like protein [H]

Number of amino acids: Translated: 356; Mature: 355

Protein sequence:

>356_residues
MSKTKQLIEEASHFITICYKELSKEHFIEERMKEIQAEIEKTGTYEHTFEELVHGSRMAWRNSNRCIGRLFWSKMHILDA
REVNDEEGVYHALIHHIKYATNDGKVKPTITIFKQYQGEENNIRIYNHQLIRYAGYKTEMGVTGDSHSTAFTDFCQELGW
QGEGTNFDVLPLVFSIDGKAPIYKEIPKEEVKEVPIEHPEYPISSLGAKWYGVPMISDMRLEIGGISYTAAPFNGWYMGT
EIGARNLADHDRYNLLPAVAEMMDLDTSRNGTLWKDKALIELNVAVLHSFKKQGVSIVDHHTAAQQFQQFEKQEAACGRV
VTGNWVWLIPPLSPATTHIYHKPYPNEILKPNFFHK

Sequences:

>Translated_356_residues
MSKTKQLIEEASHFITICYKELSKEHFIEERMKEIQAEIEKTGTYEHTFEELVHGSRMAWRNSNRCIGRLFWSKMHILDA
REVNDEEGVYHALIHHIKYATNDGKVKPTITIFKQYQGEENNIRIYNHQLIRYAGYKTEMGVTGDSHSTAFTDFCQELGW
QGEGTNFDVLPLVFSIDGKAPIYKEIPKEEVKEVPIEHPEYPISSLGAKWYGVPMISDMRLEIGGISYTAAPFNGWYMGT
EIGARNLADHDRYNLLPAVAEMMDLDTSRNGTLWKDKALIELNVAVLHSFKKQGVSIVDHHTAAQQFQQFEKQEAACGRV
VTGNWVWLIPPLSPATTHIYHKPYPNEILKPNFFHK
>Mature_355_residues
SKTKQLIEEASHFITICYKELSKEHFIEERMKEIQAEIEKTGTYEHTFEELVHGSRMAWRNSNRCIGRLFWSKMHILDAR
EVNDEEGVYHALIHHIKYATNDGKVKPTITIFKQYQGEENNIRIYNHQLIRYAGYKTEMGVTGDSHSTAFTDFCQELGWQ
GEGTNFDVLPLVFSIDGKAPIYKEIPKEEVKEVPIEHPEYPISSLGAKWYGVPMISDMRLEIGGISYTAAPFNGWYMGTE
IGARNLADHDRYNLLPAVAEMMDLDTSRNGTLWKDKALIELNVAVLHSFKKQGVSIVDHHTAAQQFQQFEKQEAACGRVV
TGNWVWLIPPLSPATTHIYHKPYPNEILKPNFFHK

Specific function: Catalyzes the production of nitric oxide [H]

COG id: COG4362

COG function: function code PE; Nitric oxide synthase, oxygenase domain

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the NOS family. Bacterial NOS oxygenase subfamily [H]

Homologues:

Organism=Homo sapiens, GI10835173, Length=354, Percent_Identity=44.3502824858757, Blast_Score=331, Evalue=6e-91,
Organism=Homo sapiens, GI24041029, Length=356, Percent_Identity=41.2921348314607, Blast_Score=320, Evalue=2e-87,
Organism=Homo sapiens, GI40254422, Length=358, Percent_Identity=42.7374301675978, Blast_Score=318, Evalue=7e-87,
Organism=Homo sapiens, GI231571329, Length=356, Percent_Identity=42.6966292134831, Blast_Score=317, Evalue=2e-86,
Organism=Homo sapiens, GI231571356, Length=356, Percent_Identity=42.6966292134831, Blast_Score=316, Evalue=2e-86,
Organism=Homo sapiens, GI231571314, Length=356, Percent_Identity=42.6966292134831, Blast_Score=316, Evalue=2e-86,
Organism=Drosophila melanogaster, GI78706868, Length=355, Percent_Identity=43.3802816901408, Blast_Score=332, Evalue=2e-91,
Organism=Drosophila melanogaster, GI24583543, Length=355, Percent_Identity=43.3802816901408, Blast_Score=332, Evalue=2e-91,
Organism=Drosophila melanogaster, GI78706876, Length=355, Percent_Identity=43.3802816901408, Blast_Score=332, Evalue=2e-91,
Organism=Drosophila melanogaster, GI78706866, Length=355, Percent_Identity=43.3802816901408, Blast_Score=332, Evalue=2e-91,
Organism=Drosophila melanogaster, GI78706882, Length=355, Percent_Identity=43.3802816901408, Blast_Score=332, Evalue=3e-91,
Organism=Drosophila melanogaster, GI78706870, Length=355, Percent_Identity=43.3802816901408, Blast_Score=332, Evalue=3e-91,
Organism=Drosophila melanogaster, GI78706878, Length=355, Percent_Identity=43.3802816901408, Blast_Score=332, Evalue=3e-91,
Organism=Drosophila melanogaster, GI78706880, Length=355, Percent_Identity=43.3802816901408, Blast_Score=332, Evalue=3e-91,
Organism=Drosophila melanogaster, GI78706872, Length=154, Percent_Identity=40.9090909090909, Blast_Score=129, Evalue=3e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017142
- InterPro:   IPR004030 [H]

Pfam domain/function: PF02898 NO_synthase [H]

EC number: 1.14.13.39

Molecular weight: Translated: 40948; Mature: 40817

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: PS60001 NOS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKTKQLIEEASHFITICYKELSKEHFIEERMKEIQAEIEKTGTYEHTFEELVHGSRMAW
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCHHEE
RNSNRCIGRLFWSKMHILDAREVNDEEGVYHALIHHIKYATNDGKVKPTITIFKQYQGEE
CCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCCCEEEEEEHHCCCC
NNIRIYNHQLIRYAGYKTEMGVTGDSHSTAFTDFCQELGWQGEGTNFDVLPLVFSIDGKA
CCEEEEEEEEEEEECCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCEEEEEEEEEECCCC
PIYKEIPKEEVKEVPIEHPEYPISSLGAKWYGVPMISDMRLEIGGISYTAAPFNGWYMGT
CHHHHCCHHHHHHCCCCCCCCCHHHCCCCEECCCCEECCEEEECCEEEEECCCCCEEECC
EIGARNLADHDRYNLLPAVAEMMDLDTSRNGTLWKDKALIELNVAVLHSFKKQGVSIVDH
HHCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCEEECCEEEEEHHHHHHHHHHCCCEEEHH
HTAAQQFQQFEKQEAACGRVVTGNWVWLIPPLSPATTHIYHKPYPNEILKPNFFHK
HHHHHHHHHHHHHHHHCCCEEECCEEEEECCCCCCCCEEECCCCCCCCCCCCCCCC
>Mature Secondary Structure 
SKTKQLIEEASHFITICYKELSKEHFIEERMKEIQAEIEKTGTYEHTFEELVHGSRMAW
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCHHEE
RNSNRCIGRLFWSKMHILDAREVNDEEGVYHALIHHIKYATNDGKVKPTITIFKQYQGEE
CCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCCCEEEEEEHHCCCC
NNIRIYNHQLIRYAGYKTEMGVTGDSHSTAFTDFCQELGWQGEGTNFDVLPLVFSIDGKA
CCEEEEEEEEEEEECCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCEEEEEEEEEECCCC
PIYKEIPKEEVKEVPIEHPEYPISSLGAKWYGVPMISDMRLEIGGISYTAAPFNGWYMGT
CHHHHCCHHHHHHCCCCCCCCCHHHCCCCEECCCCEECCEEEECCEEEEECCCCCEEECC
EIGARNLADHDRYNLLPAVAEMMDLDTSRNGTLWKDKALIELNVAVLHSFKKQGVSIVDH
HHCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCEEECCEEEEEHHHHHHHHHHCCCEEEHH
HTAAQQFQQFEKQEAACGRVVTGNWVWLIPPLSPATTHIYHKPYPNEILKPNFFHK
HHHHHHHHHHHHHHHHCCCEEECCEEEEECCCCCCCCEEECCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: L-arginine; NADPH; H+; O2

Specific reaction: L-arginine + n NADPH + n H+ + m O2 = citrulline + nitric oxide + n NADP+

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9272861; 9384377; 11856757; 12220171 [H]