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The map label for this gene is dacA [H]

Identifier: 49188225

GI number: 49188225

Start: 5125708

End: 5126979

Strand: Direct

Name: dacA [H]

Synonym: BAS5240

Alternate gene names: 49188225

Gene position: 5125708-5126979 (Clockwise)

Preceding gene: 49188224

Following gene: 49188226

Centisome position: 98.03

GC content: 35.38

Gene sequence:

>1272_bases
ATGAAGAAAATCTTGTCTATTTTACTAGCGCTTTTATTGTCAATTTCTTCCTTAGGAGTAACAACATCCCATGCAGAAGA
GAAAATACACATTGAGGCGGCGGCTGCACTTCTATTTGATGCAGATACAGGAAAAATACTGCATGAACAAAATCCTGATG
AATTACTAGCTATCGCTAGTATGTCAAAGTTAATTGTTGTTTATGCTGTATTAGAAGCAATTAAAGAAGGAAAAATCACA
TGGGATACAAAGGTAAACATTTCTGATTATGCTTATGAAGTTTCACGTAATAACGAATTCTCTAACGTACCGTTCGAAAA
AGGACGTCAATATACTGTAAGAGAACTATATCATTCTATCGTTATCTTCTCTGCAAACGGATCTAGTATTGCCTTAGCAG
AGCTTCTTGCAGGAAGTGAGAAAAACTTCTTAAACCTTGCAAATGAACATGCAAAAAAACTAGGGTTAAAGAAATATAAA
TTTGTAAACGCTACTGGTTTAAATAACACTGACTTAAAAGGAAAACATCCTGAGGGTACTGATCCAAATGCAGAAAACTC
TATGTCAGCTCGCGATATGGGTATACTTTCAAAAGCAATGATTACAAAGTATCCGGAAATGCTAGAGGATACAAAACAAA
GATTTAGAAACTTCCCAGATAATCATCCGAAACCAATTCGTATGGAAAACTGGAACTGGATGTTACCAGGGGCTGCTTTC
GCTTATGAAGGTACTGATGGTTTAAAAACCGGAAGTTCTGATACAGCTGGATACGGATTTACCATTACTGCTAAACGTGG
TGATGTACGTCTTATTTCGGTTATTATTAAAACAAAATCAATGGACGAGCGCTTCACAGAATCTCGTGAATTAATCGAAT
ACGGGTTTAATAACTTTGAAAAACAAAAGCTAAAGGTTGATAAAAACAATACACTCTCTGTCGCACAAGGGAAAGAGGAT
CAAGTAACTGTTGCACCGGAAAAAGAAATAACCGTAATTGCGAAGAAAGGTAGCAAAAATCCTTATAAAATTGGAACTGA
AGTAGATAAATCTCTTGCTGAAGATGGGCATTTAGTTGCTCCTATTAAGAAGGATGCCAAAGTAGGCTCAATTACTTTAG
AATCAACTGATAAATATGGTTTCTTAGACGGTAGTAACAGTATGAAAGTTACTGCAAAAACGACAGAAGAAGTTGAAAAA
GCAAATTGGTTTGTTTTAACAATGCGCTCTATTGGAGATTTCTTCTCAAACTTATGGTCTAAAGTTTTTTAA

Upstream 100 bases:

>100_bases
CTTTTCAAATTTCTTGTAAAATTGTAACCTTTTCATATCCAAATTTATGCTAAAATGGTACGAGCTACATGCTAATAACG
ATGCGAAGGTGATAATTTAT

Downstream 100 bases:

>100_bases
TAAAAAAGCTGGCTCACGCTAGCTTTTTTTATTTTCCACTCTCATTGTAAAACTTCGTTTGGTTGTCATATCTGAGTCCT
TTTTCTCATACGTATGAACT

Product: D-alanyl-D-alanine carboxypeptidase

Products: NA

Alternate protein names: CPase; DD-carboxypeptidase; DD-peptidase; Penicillin-binding protein 5; PBP-5 [H]

Number of amino acids: Translated: 423; Mature: 423

Protein sequence:

>423_residues
MKKILSILLALLLSISSLGVTTSHAEEKIHIEAAAALLFDADTGKILHEQNPDELLAIASMSKLIVVYAVLEAIKEGKIT
WDTKVNISDYAYEVSRNNEFSNVPFEKGRQYTVRELYHSIVIFSANGSSIALAELLAGSEKNFLNLANEHAKKLGLKKYK
FVNATGLNNTDLKGKHPEGTDPNAENSMSARDMGILSKAMITKYPEMLEDTKQRFRNFPDNHPKPIRMENWNWMLPGAAF
AYEGTDGLKTGSSDTAGYGFTITAKRGDVRLISVIIKTKSMDERFTESRELIEYGFNNFEKQKLKVDKNNTLSVAQGKED
QVTVAPEKEITVIAKKGSKNPYKIGTEVDKSLAEDGHLVAPIKKDAKVGSITLESTDKYGFLDGSNSMKVTAKTTEEVEK
ANWFVLTMRSIGDFFSNLWSKVF

Sequences:

>Translated_423_residues
MKKILSILLALLLSISSLGVTTSHAEEKIHIEAAAALLFDADTGKILHEQNPDELLAIASMSKLIVVYAVLEAIKEGKIT
WDTKVNISDYAYEVSRNNEFSNVPFEKGRQYTVRELYHSIVIFSANGSSIALAELLAGSEKNFLNLANEHAKKLGLKKYK
FVNATGLNNTDLKGKHPEGTDPNAENSMSARDMGILSKAMITKYPEMLEDTKQRFRNFPDNHPKPIRMENWNWMLPGAAF
AYEGTDGLKTGSSDTAGYGFTITAKRGDVRLISVIIKTKSMDERFTESRELIEYGFNNFEKQKLKVDKNNTLSVAQGKED
QVTVAPEKEITVIAKKGSKNPYKIGTEVDKSLAEDGHLVAPIKKDAKVGSITLESTDKYGFLDGSNSMKVTAKTTEEVEK
ANWFVLTMRSIGDFFSNLWSKVF
>Mature_423_residues
MKKILSILLALLLSISSLGVTTSHAEEKIHIEAAAALLFDADTGKILHEQNPDELLAIASMSKLIVVYAVLEAIKEGKIT
WDTKVNISDYAYEVSRNNEFSNVPFEKGRQYTVRELYHSIVIFSANGSSIALAELLAGSEKNFLNLANEHAKKLGLKKYK
FVNATGLNNTDLKGKHPEGTDPNAENSMSARDMGILSKAMITKYPEMLEDTKQRFRNFPDNHPKPIRMENWNWMLPGAAF
AYEGTDGLKTGSSDTAGYGFTITAKRGDVRLISVIIKTKSMDERFTESRELIEYGFNNFEKQKLKVDKNNTLSVAQGKED
QVTVAPEKEITVIAKKGSKNPYKIGTEVDKSLAEDGHLVAPIKKDAKVGSITLESTDKYGFLDGSNSMKVTAKTTEEVEK
ANWFVLTMRSIGDFFSNLWSKVF

Specific function: Removes C-terminal D-alanyl residues from sugar-peptide cell wall precursors [H]

COG id: COG1686

COG function: function code M; D-alanyl-D-alanine carboxypeptidase

Gene ontology:

Cell location: Secreted, cell wall [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S11 family [H]

Homologues:

Organism=Escherichia coli, GI1787062, Length=417, Percent_Identity=31.1750599520384, Blast_Score=145, Evalue=5e-36,
Organism=Escherichia coli, GI1786851, Length=410, Percent_Identity=29.2682926829268, Blast_Score=140, Evalue=2e-34,
Organism=Escherichia coli, GI87082022, Length=429, Percent_Identity=27.5058275058275, Blast_Score=124, Evalue=8e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR015956
- InterPro:   IPR018044
- InterPro:   IPR012907
- InterPro:   IPR001967 [H]

Pfam domain/function: PF07943 PBP5_C; PF00768 Peptidase_S11 [H]

EC number: =3.4.16.4 [H]

Molecular weight: Translated: 47014; Mature: 47014

Theoretical pI: Translated: 7.91; Mature: 7.91

Prosite motif: PS00018 EF_HAND_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKILSILLALLLSISSLGVTTSHAEEKIHIEAAAALLFDADTGKILHEQNPDELLAIAS
CHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEEEEEEEECCCCCEECCCCHHHHHHHHH
MSKLIVVYAVLEAIKEGKITWDTKVNISDYAYEVSRNNEFSNVPFEKGRQYTVRELYHSI
HHHHHHHHHHHHHHHCCCEEEEEEECCHHHEEEECCCCCCCCCCHHCCCCHHHHHHHCEE
VIFSANGSSIALAELLAGSEKNFLNLANEHAKKLGLKKYKFVNATGLNNTDLKGKHPEGT
EEEECCCCCCHHHHHHCCCCCHHHHHHHHHHHHCCCHHEEEEECCCCCCCCCCCCCCCCC
DPNAENSMSARDMGILSKAMITKYPEMLEDTKQRFRNFPDNHPKPIRMENWNWMLPGAAF
CCCCCCCCCHHHHHHHHHHHHHHCHHHHHHHHHHHHCCCCCCCCCEEECCCCEECCCCEE
AYEGTDGLKTGSSDTAGYGFTITAKRGDVRLISVIIKTKSMDERFTESRELIEYGFNNFE
EECCCCCCCCCCCCCCCCCEEEEECCCCEEEEEEEEHHCCHHHHHHHHHHHHHHCCCCCC
KQKLKVDKNNTLSVAQGKEDQVTVAPEKEITVIAKKGSKNPYKIGTEVDKSLAEDGHLVA
CHHEEECCCCEEEEECCCCCCEEECCCCCEEEEEECCCCCCEECCCHHHHHHHCCCCEEE
PIKKDAKVGSITLESTDKYGFLDGSNSMKVTAKTTEEVEKANWFVLTMRSIGDFFSNLWS
ECCCCCCCCEEEEECCCCEEEEECCCCEEEEECCHHHHHHCCEEEEEHHHHHHHHHHHHH
KVF
HCC
>Mature Secondary Structure
MKKILSILLALLLSISSLGVTTSHAEEKIHIEAAAALLFDADTGKILHEQNPDELLAIAS
CHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEEEEEEEECCCCCEECCCCHHHHHHHHH
MSKLIVVYAVLEAIKEGKITWDTKVNISDYAYEVSRNNEFSNVPFEKGRQYTVRELYHSI
HHHHHHHHHHHHHHHCCCEEEEEEECCHHHEEEECCCCCCCCCCHHCCCCHHHHHHHCEE
VIFSANGSSIALAELLAGSEKNFLNLANEHAKKLGLKKYKFVNATGLNNTDLKGKHPEGT
EEEECCCCCCHHHHHHCCCCCHHHHHHHHHHHHCCCHHEEEEECCCCCCCCCCCCCCCCC
DPNAENSMSARDMGILSKAMITKYPEMLEDTKQRFRNFPDNHPKPIRMENWNWMLPGAAF
CCCCCCCCCHHHHHHHHHHHHHHCHHHHHHHHHHHHCCCCCCCCCEEECCCCEECCCCEE
AYEGTDGLKTGSSDTAGYGFTITAKRGDVRLISVIIKTKSMDERFTESRELIEYGFNNFE
EECCCCCCCCCCCCCCCCCEEEEECCCCEEEEEEEEHHCCHHHHHHHHHHHHHHCCCCCC
KQKLKVDKNNTLSVAQGKEDQVTVAPEKEITVIAKKGSKNPYKIGTEVDKSLAEDGHLVA
CHHEEECCCCEEEEECCCCCCEEECCCCCEEEEEECCCCCCEECCCHHHHHHHCCCCEEE
PIKKDAKVGSITLESTDKYGFLDGSNSMKVTAKTTEEVEKANWFVLTMRSIGDFFSNLWS
ECCCCCCCCEEEEECCCCEEEEECCCCEEEEECCHHHHHHCCEEEEEHHHHHHHHHHHHH
KVF
HCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8370539; 6780559; 111240 [H]