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The map label for this gene is pbpG [H]

Identifier: 49188209

GI number: 49188209

Start: 5111034

End: 5113085

Strand: Direct

Name: pbpG [H]

Synonym: BAS5223

Alternate gene names: 49188209

Gene position: 5111034-5113085 (Clockwise)

Preceding gene: 49188208

Following gene: 49188212

Centisome position: 97.75

GC content: 38.35

Gene sequence:

>2052_bases
ATGGATCAAACTATGAATCCGAAACTTAAAAAATATAAACGTCTTTTCTTCACCGTAATGTTTTCTTCTGTTCTTTTTTT
CGTTTTCTCCTTTTTTGTTATTATTATAGTTGCAAAAATTATGGGACCTCCTCCTGTTGCTGTCCCGCAAACAAGTGTCT
TTTACGCTAATGATGACACTGTTATAGGACAAAGTAATCAGGTACAAAAACGCTACAATGTATCTCTCAATGAAATTTCT
CCTTATGTAAAAGAGGCAACACTATCTATCGAAGATCAACGATTCTACAAACATCATGGCTTTGATATGAAGCGTATTGC
TGGTGCCATTGTTGCCGATTTAAAAGCGATGGCAAAGGTCCAAGGTGCTAGTACGATTACACAACAATACGCACGTAATC
TGTATTTAGATCATGATAAAACGTGGAAACGTAAACTATTAGAAGCAATGTACACAATCCGCCTTGAAGTAAACTATAAT
AAAAATCATATTTTAGAAGGATATTTAAATACAATTTATTACGGGCATGGTGCTTACGGAATCGAAGCTGCGTCTCGTCT
ATATTTCGATAAAACAGCAAAAGAATTAACATTAGCGGAGGCTAGTATGCTCGCAGGTATCCCGAAAGGACCTAGTGTCT
ATTCTCCCTTTTTAAAAGAAGATCGTGCGAAAGGACGACAATCTCTCATATTAGATGAAATGGTAGAACAAGGTTATATT
ACGAAGAAACAAGCAGCTTCAGCGAAGAAGGAGCCACTAACTTTCGCCTCATTAGATACGAAAAAGGTGGCAGAGGTTGC
ACCTTATTTCCAAGATGCTGTACAAGCTTCTCTTCTTCGTGATGTCGGATTAGATGAGCAAGCGTTACAAAAAGGTGGCT
TGCGTATTTATACAACACTAGACCCGAAATTACAATCGGTTGCAGAGCAAGCTGTAAAAGACCACATACCTGACACAACA
AACATACAAACCGCTCTTGTCTCTATGAATCCAAAAACAGGTGAAGTAGCTGCCCTTGTTGGTGGAACTGATTATAATAC
GAGTCAATTTAACAGGGCTACACAGGGCGCTCGCCAGCCTGGTTCTACGTTTAAGCCATTCCTATATTACGCGGCATTAG
AACGAGGATTCACCCCTGCTACGCGCTTAAAAAGCGAATACACTGTTTTCACTTTAGGTGATGGTGTTTCAAAGTATAAA
CCGAAAAACTATAAGGACTATTATGCAGACGATTTTGTAACAATGGCGCAAGCTCTTGCAGTCTCTGATAACGTATACGC
CGTGAAGACCAATTTATTTTTAGGCGAAGACATTCTTACAAAAACAGCGAAGCAATTCGGAATTAAGAGTGCATTAAAAG
ATGTACCGTCTCTTGCTCTCGGCACATCTCCTGTAAAACCAATTGAAATGGTTAACGCTTATAGCATGTTCGCAAACGGT
GGAAAAGAAGTAAAACCTACTTTCATTCGCCGCATTATGGATCATGAAGGCAATATATTATACGATGCTCATTTAGAGAG
TAAACAAGTTCTTGATAAGAACAAAGCGTTTGTAATGGAAGAAATGATGACAGGCATGTTTAATAAAAAACTAAGTAGTT
ATGCCGCTGTAACTGGTCAATCGATGTTATCTAAACTATCCAGAACCTATGCCGGAAAATCTGGTTCTACTGAAACAGAT
AGTTGGATGATTGGGTTTACCCCGCAAATCGTAACGGGTGTATGGGTTGGATATGATCAACCAAAATCAATTTCAAACGT
AGCAGAACAAGGATATGCGAAAAGAATATGGACCGATACGATGGAGAAAGGCTTAGATGGACAACCTAAAAAAGAATTTA
AACGGCCAAGTGACGTCGTTGCAGTCGATATTAACCCTGAAAACGGGAAAATCGCTACAAAAAATTGTCCAATTTCAGTG
AAAATGTATTTTGCTAAAGGTACCGAGCCTACAGAATATTGTATGGATCATGTCGATGATAAAGAAGAATTTGAAAAGAC
AAGTGAAGAAAAGAAAAAAACAAGTTGGTGGAAAAAATATCTTCCTTGGTGA

Upstream 100 bases:

>100_bases
ATTGAAGGAGCTTTTTGTTTTCAAAATTTCATTGTTTAAAATAACCGAAAACCCTCAATACTAATACAATAATGTTTGTA
TGAATGAGGGGCTGAAAAAG

Downstream 100 bases:

>100_bases
AACACGAGGCTATTCCAATAAAATGTGGAATAGCCTTTATTGTCATTCTTTGTCGGTAAATCGATATTCCTTGTCGAATC
GTCGATATATTAAAAAAATC

Product: penicillin-binding protein

Products: NA

Alternate protein names: PBP-2D; PBP2d [H]

Number of amino acids: Translated: 683; Mature: 683

Protein sequence:

>683_residues
MDQTMNPKLKKYKRLFFTVMFSSVLFFVFSFFVIIIVAKIMGPPPVAVPQTSVFYANDDTVIGQSNQVQKRYNVSLNEIS
PYVKEATLSIEDQRFYKHHGFDMKRIAGAIVADLKAMAKVQGASTITQQYARNLYLDHDKTWKRKLLEAMYTIRLEVNYN
KNHILEGYLNTIYYGHGAYGIEAASRLYFDKTAKELTLAEASMLAGIPKGPSVYSPFLKEDRAKGRQSLILDEMVEQGYI
TKKQAASAKKEPLTFASLDTKKVAEVAPYFQDAVQASLLRDVGLDEQALQKGGLRIYTTLDPKLQSVAEQAVKDHIPDTT
NIQTALVSMNPKTGEVAALVGGTDYNTSQFNRATQGARQPGSTFKPFLYYAALERGFTPATRLKSEYTVFTLGDGVSKYK
PKNYKDYYADDFVTMAQALAVSDNVYAVKTNLFLGEDILTKTAKQFGIKSALKDVPSLALGTSPVKPIEMVNAYSMFANG
GKEVKPTFIRRIMDHEGNILYDAHLESKQVLDKNKAFVMEEMMTGMFNKKLSSYAAVTGQSMLSKLSRTYAGKSGSTETD
SWMIGFTPQIVTGVWVGYDQPKSISNVAEQGYAKRIWTDTMEKGLDGQPKKEFKRPSDVVAVDINPENGKIATKNCPISV
KMYFAKGTEPTEYCMDHVDDKEEFEKTSEEKKKTSWWKKYLPW

Sequences:

>Translated_683_residues
MDQTMNPKLKKYKRLFFTVMFSSVLFFVFSFFVIIIVAKIMGPPPVAVPQTSVFYANDDTVIGQSNQVQKRYNVSLNEIS
PYVKEATLSIEDQRFYKHHGFDMKRIAGAIVADLKAMAKVQGASTITQQYARNLYLDHDKTWKRKLLEAMYTIRLEVNYN
KNHILEGYLNTIYYGHGAYGIEAASRLYFDKTAKELTLAEASMLAGIPKGPSVYSPFLKEDRAKGRQSLILDEMVEQGYI
TKKQAASAKKEPLTFASLDTKKVAEVAPYFQDAVQASLLRDVGLDEQALQKGGLRIYTTLDPKLQSVAEQAVKDHIPDTT
NIQTALVSMNPKTGEVAALVGGTDYNTSQFNRATQGARQPGSTFKPFLYYAALERGFTPATRLKSEYTVFTLGDGVSKYK
PKNYKDYYADDFVTMAQALAVSDNVYAVKTNLFLGEDILTKTAKQFGIKSALKDVPSLALGTSPVKPIEMVNAYSMFANG
GKEVKPTFIRRIMDHEGNILYDAHLESKQVLDKNKAFVMEEMMTGMFNKKLSSYAAVTGQSMLSKLSRTYAGKSGSTETD
SWMIGFTPQIVTGVWVGYDQPKSISNVAEQGYAKRIWTDTMEKGLDGQPKKEFKRPSDVVAVDINPENGKIATKNCPISV
KMYFAKGTEPTEYCMDHVDDKEEFEKTSEEKKKTSWWKKYLPW
>Mature_683_residues
MDQTMNPKLKKYKRLFFTVMFSSVLFFVFSFFVIIIVAKIMGPPPVAVPQTSVFYANDDTVIGQSNQVQKRYNVSLNEIS
PYVKEATLSIEDQRFYKHHGFDMKRIAGAIVADLKAMAKVQGASTITQQYARNLYLDHDKTWKRKLLEAMYTIRLEVNYN
KNHILEGYLNTIYYGHGAYGIEAASRLYFDKTAKELTLAEASMLAGIPKGPSVYSPFLKEDRAKGRQSLILDEMVEQGYI
TKKQAASAKKEPLTFASLDTKKVAEVAPYFQDAVQASLLRDVGLDEQALQKGGLRIYTTLDPKLQSVAEQAVKDHIPDTT
NIQTALVSMNPKTGEVAALVGGTDYNTSQFNRATQGARQPGSTFKPFLYYAALERGFTPATRLKSEYTVFTLGDGVSKYK
PKNYKDYYADDFVTMAQALAVSDNVYAVKTNLFLGEDILTKTAKQFGIKSALKDVPSLALGTSPVKPIEMVNAYSMFANG
GKEVKPTFIRRIMDHEGNILYDAHLESKQVLDKNKAFVMEEMMTGMFNKKLSSYAAVTGQSMLSKLSRTYAGKSGSTETD
SWMIGFTPQIVTGVWVGYDQPKSISNVAEQGYAKRIWTDTMEKGLDGQPKKEFKRPSDVVAVDINPENGKIATKNCPISV
KMYFAKGTEPTEYCMDHVDDKEEFEKTSEEKKKTSWWKKYLPW

Specific function: Involved in the polymerization and cross-linking of spore peptidoglycan. May be required for synthesis of the spore germ cell wall, the first layer of peptidoglycan synthesized on the surface of the inner forespore membrane [H]

COG id: COG0744

COG function: function code M; Membrane carboxypeptidase (penicillin-binding protein)

Gene ontology:

Cell location: Cell membrane; Single-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the transpeptidase family [H]

Homologues:

Organism=Escherichia coli, GI1786343, Length=537, Percent_Identity=29.2364990689013, Blast_Score=202, Evalue=4e-53,
Organism=Escherichia coli, GI87082258, Length=265, Percent_Identity=39.2452830188679, Blast_Score=191, Evalue=1e-49,
Organism=Escherichia coli, GI1788867, Length=605, Percent_Identity=27.4380165289256, Blast_Score=174, Evalue=2e-44,
Organism=Escherichia coli, GI1789601, Length=165, Percent_Identity=38.7878787878788, Blast_Score=126, Evalue=5e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR001264
- InterPro:   IPR011816
- InterPro:   IPR001460 [H]

Pfam domain/function: PF00912 Transgly; PF00905 Transpeptidase [H]

EC number: 2.4.1.129; 3.4.-.- [C]

Molecular weight: Translated: 76656; Mature: 76656

Theoretical pI: Translated: 9.49; Mature: 9.49

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDQTMNPKLKKYKRLFFTVMFSSVLFFVFSFFVIIIVAKIMGPPPVAVPQTSVFYANDDT
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCCE
VIGQSNQVQKRYNVSLNEISPYVKEATLSIEDQRFYKHHGFDMKRIAGAIVADLKAMAKV
EECCCHHHHHHHCCCHHHHCHHHHHHHCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHH
QGASTITQQYARNLYLDHDKTWKRKLLEAMYTIRLEVNYNKNHILEGYLNTIYYGHGAYG
CCHHHHHHHHHHHCCCCCCHHHHHHHHHHHEEEEEEEECCCCHHHHHHHHHEEECCCCHH
IEAASRLYFDKTAKELTLAEASMLAGIPKGPSVYSPFLKEDRAKGRQSLILDEMVEQGYI
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHCHHHHHHHHCHHHHHHHHHHHHCCCC
TKKQAASAKKEPLTFASLDTKKVAEVAPYFQDAVQASLLRDVGLDEQALQKGGLRIYTTL
CHHHHHCCCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCEEEEEEC
DPKLQSVAEQAVKDHIPDTTNIQTALVSMNPKTGEVAALVGGTDYNTSQFNRATQGARQP
CHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCEEEEECCCCCCHHHHHHHHHCCCCC
GSTFKPFLYYAALERGFTPATRLKSEYTVFTLGDGVSKYKPKNYKDYYADDFVTMAQALA
CCCCHHHHHHHHHHHCCCCHHHCCCCEEEEEECCCHHHCCCCCCCCHHHHHHHHHHHHHH
VSDNVYAVKTNLFLGEDILTKTAKQFGIKSALKDVPSLALGTSPVKPIEMVNAYSMFANG
HCCCEEEEEECEEECHHHHHHHHHHHHHHHHHHHCCHHHCCCCCCCHHHHHHHHHHHHCC
GKEVKPTFIRRIMDHEGNILYDAHLESKQVLDKNKAFVMEEMMTGMFNKKLSSYAAVTGQ
CCCCCHHHHHHHHCCCCCEEEECCCCHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHH
SMLSKLSRTYAGKSGSTETDSWMIGFTPQIVTGVWVGYDQPKSISNVAEQGYAKRIWTDT
HHHHHHHHHHCCCCCCCCCCCEEEECCHHHHHHEEECCCCCHHHHHHHHHHHHHHHHHHH
MEKGLDGQPKKEFKRPSDVVAVDINPENGKIATKNCPISVKMYFAKGTEPTEYCMDHVDD
HHCCCCCCCHHHHCCCCCEEEEEECCCCCEEEECCCCEEEEEEEECCCCHHHHHHHCCCC
KEEFEKTSEEKKKTSWWKKYLPW
HHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MDQTMNPKLKKYKRLFFTVMFSSVLFFVFSFFVIIIVAKIMGPPPVAVPQTSVFYANDDT
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCCE
VIGQSNQVQKRYNVSLNEISPYVKEATLSIEDQRFYKHHGFDMKRIAGAIVADLKAMAKV
EECCCHHHHHHHCCCHHHHCHHHHHHHCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHH
QGASTITQQYARNLYLDHDKTWKRKLLEAMYTIRLEVNYNKNHILEGYLNTIYYGHGAYG
CCHHHHHHHHHHHCCCCCCHHHHHHHHHHHEEEEEEEECCCCHHHHHHHHHEEECCCCHH
IEAASRLYFDKTAKELTLAEASMLAGIPKGPSVYSPFLKEDRAKGRQSLILDEMVEQGYI
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHCHHHHHHHHCHHHHHHHHHHHHCCCC
TKKQAASAKKEPLTFASLDTKKVAEVAPYFQDAVQASLLRDVGLDEQALQKGGLRIYTTL
CHHHHHCCCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCEEEEEEC
DPKLQSVAEQAVKDHIPDTTNIQTALVSMNPKTGEVAALVGGTDYNTSQFNRATQGARQP
CHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCEEEEECCCCCCHHHHHHHHHCCCCC
GSTFKPFLYYAALERGFTPATRLKSEYTVFTLGDGVSKYKPKNYKDYYADDFVTMAQALA
CCCCHHHHHHHHHHHCCCCHHHCCCCEEEEEECCCHHHCCCCCCCCHHHHHHHHHHHHHH
VSDNVYAVKTNLFLGEDILTKTAKQFGIKSALKDVPSLALGTSPVKPIEMVNAYSMFANG
HCCCEEEEEECEEECHHHHHHHHHHHHHHHHHHHCCHHHCCCCCCCHHHHHHHHHHHHCC
GKEVKPTFIRRIMDHEGNILYDAHLESKQVLDKNKAFVMEEMMTGMFNKKLSSYAAVTGQ
CCCCCHHHHHHHHCCCCCEEEECCCCHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHH
SMLSKLSRTYAGKSGSTETDSWMIGFTPQIVTGVWVGYDQPKSISNVAEQGYAKRIWTDT
HHHHHHHHHHCCCCCCCCCCCEEEECCHHHHHHEEECCCCCHHHHHHHHHHHHHHHHHHH
MEKGLDGQPKKEFKRPSDVVAVDINPENGKIATKNCPISVKMYFAKGTEPTEYCMDHVDD
HHCCCCCCCHHHHCCCCCEEEEEECCCCCEEEECCCCEEEEEEEECCCCHHHHHHHCCCC
KEEFEKTSEEKKKTSWWKKYLPW
HHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferases; Acyltransferases; Aminoacyltransferases [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9353933; 9384377 [H]