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The map label for this gene is mapB [H]

Identifier: 49188190

GI number: 49188190

Start: 5087559

End: 5088305

Strand: Direct

Name: mapB [H]

Synonym: BAS5204

Alternate gene names: 49188190

Gene position: 5087559-5088305 (Clockwise)

Preceding gene: 49188183

Following gene: 49188191

Centisome position: 97.3

GC content: 38.69

Gene sequence:

>747_bases
ATGATTATTCGTAATGAACAAGATTTAGAAGGCTTACGAAAAATCGGCCGCATCGTTGCGCTTGCACGTGAAGAAATGAA
AAAAGAAGCGAAGCCAGGTATGACAACGAAAGAGCTTGATTTGATCGGTAAAAAAGTATTAGATGAGCATGGTGCAATTT
CTGCACCTGAAAAAGAATATGATTTCCCTGGTGTAACTTGCATCAGTGTAAACGAAGAAGTTGCTCACGGTATTCCAGGT
GATCGCGTATTAAAAGAAGGCGACCTTGTAAATGTCGACGTATCTGCAGCACTTGATGGTTATTATGCAGATACAGGTAT
TTCATTTGTACTTGGAGAAGATGAAGCAAAAGAAAAGCTTTGCCAAGCAGCTGTTGATGCCTTTTGGGCAGCAATGAAAA
AAGTGAAAGCTGGTTCAAAACAAAACCAAATTGGTCGTGCTGTTTCAAACTTTGCACATAAAAATGGATACAATGTTATT
CAAAATTTAACTGGTCACGGTATTGGTCTTAGCTTACATGAAGCACCAAACCATATTTTAAGTTACTTTGACCCAATGGA
TAATGCGCTTCTAAAAGACGGTCTTGTTATCGCTGTAGAACCATTTATTTCTATGAAAGCTGATCACATTATCGAACGTG
GCGATGATGGTTGGACATTCGTTACACCTGATAAAAGTCTTGTTGCACAATGTGAACATACAGTTGTCGTAACTCGCGGC
GAGCCGATTATTTTAACAGAAATATAA

Upstream 100 bases:

>100_bases
ATGAGACGAATCAACCCGCATACTGCAAGCGAGTAGCAGGACGAAAATAAACATGATACAATCGGGTAAGATATATATTC
GAGGAAGGACAGTGGTTTTC

Downstream 100 bases:

>100_bases
AATTAAAAATGACCGTTTTTTAGAATTTTTCTACTAAAACGGTCATTTTTAATTTTTGGAAAATATTTGTTAAACCGCTG
TTGAACTCTTTATCATTTAA

Product: methionine aminopeptidase

Products: NA

Alternate protein names: MAP 2 [H]

Number of amino acids: Translated: 248; Mature: 248

Protein sequence:

>248_residues
MIIRNEQDLEGLRKIGRIVALAREEMKKEAKPGMTTKELDLIGKKVLDEHGAISAPEKEYDFPGVTCISVNEEVAHGIPG
DRVLKEGDLVNVDVSAALDGYYADTGISFVLGEDEAKEKLCQAAVDAFWAAMKKVKAGSKQNQIGRAVSNFAHKNGYNVI
QNLTGHGIGLSLHEAPNHILSYFDPMDNALLKDGLVIAVEPFISMKADHIIERGDDGWTFVTPDKSLVAQCEHTVVVTRG
EPIILTEI

Sequences:

>Translated_248_residues
MIIRNEQDLEGLRKIGRIVALAREEMKKEAKPGMTTKELDLIGKKVLDEHGAISAPEKEYDFPGVTCISVNEEVAHGIPG
DRVLKEGDLVNVDVSAALDGYYADTGISFVLGEDEAKEKLCQAAVDAFWAAMKKVKAGSKQNQIGRAVSNFAHKNGYNVI
QNLTGHGIGLSLHEAPNHILSYFDPMDNALLKDGLVIAVEPFISMKADHIIERGDDGWTFVTPDKSLVAQCEHTVVVTRG
EPIILTEI
>Mature_248_residues
MIIRNEQDLEGLRKIGRIVALAREEMKKEAKPGMTTKELDLIGKKVLDEHGAISAPEKEYDFPGVTCISVNEEVAHGIPG
DRVLKEGDLVNVDVSAALDGYYADTGISFVLGEDEAKEKLCQAAVDAFWAAMKKVKAGSKQNQIGRAVSNFAHKNGYNVI
QNLTGHGIGLSLHEAPNHILSYFDPMDNALLKDGLVIAVEPFISMKADHIIERGDDGWTFVTPDKSLVAQCEHTVVVTRG
EPIILTEI

Specific function: Removes the amino-terminal methionine from nascent proteins [H]

COG id: COG0024

COG function: function code J; Methionine aminopeptidase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M24A family [H]

Homologues:

Organism=Homo sapiens, GI164420681, Length=247, Percent_Identity=36.8421052631579, Blast_Score=149, Evalue=2e-36,
Organism=Homo sapiens, GI40385867, Length=247, Percent_Identity=32.3886639676113, Blast_Score=137, Evalue=1e-32,
Organism=Escherichia coli, GI1786364, Length=247, Percent_Identity=38.4615384615385, Blast_Score=171, Evalue=3e-44,
Organism=Escherichia coli, GI1788728, Length=246, Percent_Identity=24.7967479674797, Blast_Score=68, Evalue=7e-13,
Organism=Caenorhabditis elegans, GI71996291, Length=247, Percent_Identity=34.0080971659919, Blast_Score=134, Evalue=4e-32,
Organism=Saccharomyces cerevisiae, GI6323273, Length=240, Percent_Identity=36.6666666666667, Blast_Score=142, Evalue=5e-35,
Organism=Drosophila melanogaster, GI21355531, Length=246, Percent_Identity=32.9268292682927, Blast_Score=134, Evalue=4e-32,
Organism=Drosophila melanogaster, GI24583427, Length=246, Percent_Identity=30.8943089430894, Blast_Score=127, Evalue=5e-30,

Paralogues:

None

Copy number: 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001714
- InterPro:   IPR000994
- InterPro:   IPR002467 [H]

Pfam domain/function: PF00557 Peptidase_M24 [H]

EC number: =3.4.11.18 [H]

Molecular weight: Translated: 27062; Mature: 27062

Theoretical pI: Translated: 4.94; Mature: 4.94

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIIRNEQDLEGLRKIGRIVALAREEMKKEAKPGMTTKELDLIGKKVLDEHGAISAPEKEY
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCCCCC
DFPGVTCISVNEEVAHGIPGDRVLKEGDLVNVDVSAALDGYYADTGISFVLGEDEAKEKL
CCCCEEEEECCHHHHCCCCCHHEECCCCEEEEEHHHHHCCEECCCCCEEEECCCHHHHHH
CQAAVDAFWAAMKKVKAGSKQNQIGRAVSNFAHKNGYNVIQNLTGHGIGLSLHEAPNHIL
HHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCHHHHHHCCCCCCEEEHHHHHHHHH
SYFDPMDNALLKDGLVIAVEPFISMKADHIIERGDDGWTFVTPDKSLVAQCEHTVVVTRG
HHHCHHHHHHHHCCEEEEECCHHHHHHHHHHHCCCCCCEEECCCHHHHHHCCCEEEEECC
EPIILTEI
CEEEEECC
>Mature Secondary Structure
MIIRNEQDLEGLRKIGRIVALAREEMKKEAKPGMTTKELDLIGKKVLDEHGAISAPEKEY
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCCCCC
DFPGVTCISVNEEVAHGIPGDRVLKEGDLVNVDVSAALDGYYADTGISFVLGEDEAKEKL
CCCCEEEEECCHHHHCCCCCHHEECCCCEEEEEHHHHHCCEECCCCCEEEECCCHHHHHH
CQAAVDAFWAAMKKVKAGSKQNQIGRAVSNFAHKNGYNVIQNLTGHGIGLSLHEAPNHIL
HHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCHHHHHHCCCCCCEEEHHHHHHHHH
SYFDPMDNALLKDGLVIAVEPFISMKADHIIERGDDGWTFVTPDKSLVAQCEHTVVVTRG
HHHCHHHHHHHHCCEEEEECCHHHHHHHHHHHCCCCCCEEECCCHHHHHHCCCEEEEECC
EPIILTEI
CEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9272861; 9384377 [H]