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The map label for this gene is glyA [H]

Identifier: 49188151

GI number: 49188151

Start: 5047590

End: 5048834

Strand: Reverse

Name: glyA [H]

Synonym: BAS5165

Alternate gene names: 49188151

Gene position: 5048834-5047590 (Counterclockwise)

Preceding gene: 49188152

Following gene: 49188150

Centisome position: 96.56

GC content: 40.0

Gene sequence:

>1245_bases
TTGGTGGATCATTTAAAACGTCAAGATGAAAAGGTATTTGCTGCAATTGAGGCAGAACTAGGAAGACAGCGTTCGAAGAT
TGAGTTAATTGCTTCGGAAAACTTCGTAAGTGAAGCGGTAATGGAGGCGCAAGGTTCTGTTTTAACGAATAAGTATGCTG
AAGGATATCCTGGGAAACGTTACTATGGTGGTTGTGAACACGTAGACGTAGTAGAAGATATCGCACGTGATCGCGTAAAA
GAAATTTTCGGCGCAGAGCACGTAAATGTTCAACCGCATTCTGGTGCACAAGCGAACATGGCAGTATACTTCACGATTTT
AGAGCAAGGCGATACAGTACTTGGTATGAATTTATCTCATGGTGGTCACTTAACACACGGAAGCCCTGTTAACTTCAGTG
GAGTACAATATAATTTCGTAGAATATGGCGTGGATGCTGAATCTCACTGTATTAATTACGATGATGTATTAGCAAAAGCG
AAAGAACATAAACCAAAATTAATCGTTGCAGGTGCAAGTGCATACCCTCGTGTTATCGATTTCAAACGATTCCGTGAGAT
TGCAGATGAAGTGGGCGCATACTTAATGGTTGATATGGCACATATCGCTGGTTTAGTAGCTGCTGGTTTACATCCAAACC
CAGTACCACATGCACATTTCGTTACAACGACAACACATAAAACGTTACGTGGCCCACGTGGTGGTATGATTTTATGTGAA
GAACAATTTGCAAAACAAATTGATAAATCAATCTTCCCTGGTATTCAAGGTGGTCCACTTATGCACGTAATCGCTGCAAA
AGCTGTTGCGTTTGGTGAGGCACTTCAAGATGATTTCAAAACATATGCACAAAATATCATTAACAATGCGAACCGCTTAG
CTGAAGGTCTTCAAAAAGAAGGACTTACACTTGTTTCTGGTGGAACAGACAATCACTTAATCTTAATTGATGTTCGTAAC
TTAGAAATCACAGGTAAAGTAGCAGAGCACGTATTAGACGAAGTTGGTATTACTGTGAACAAAAATACAATTCCATTTGA
AACAGCAAGCCCATTTGTAACAAGTGGTGTACGTATCGGTACAGCAGCTGTAACATCTCGCGGTTTCGGTTTAGAAGACA
TGGATGAAATTGCTTCACTTATTGCTTACACATTAAAAAATCATGAAAATGAAGCTGCATTAGAAGAAGCACGTAAGCGT
GTAGAAGCGTTAACGAGCAAATTCCCAATGTACACAGATCTATAA

Upstream 100 bases:

>100_bases
ATGAAGGTTCGAAAGAATTACAACCTGAATTTTCGTTTTTTCTGAATAAATAAGAAAAAACTGTTGGGAATCGATGTAAT
TCGTTCAGAAAAGGGGGATT

Downstream 100 bases:

>100_bases
TAGATTGAAGTAGACTGCTGAAACGTAATTGTTTTGGCAGTCTTTTTTGTGGACATATATTGTTCTTGAAGTATGTATAC
AAATGATGAATAAATTTTGG

Product: serine hydroxymethyltransferase

Products: NA

Alternate protein names: SHMT; Serine methylase [H]

Number of amino acids: Translated: 414; Mature: 414

Protein sequence:

>414_residues
MVDHLKRQDEKVFAAIEAELGRQRSKIELIASENFVSEAVMEAQGSVLTNKYAEGYPGKRYYGGCEHVDVVEDIARDRVK
EIFGAEHVNVQPHSGAQANMAVYFTILEQGDTVLGMNLSHGGHLTHGSPVNFSGVQYNFVEYGVDAESHCINYDDVLAKA
KEHKPKLIVAGASAYPRVIDFKRFREIADEVGAYLMVDMAHIAGLVAAGLHPNPVPHAHFVTTTTHKTLRGPRGGMILCE
EQFAKQIDKSIFPGIQGGPLMHVIAAKAVAFGEALQDDFKTYAQNIINNANRLAEGLQKEGLTLVSGGTDNHLILIDVRN
LEITGKVAEHVLDEVGITVNKNTIPFETASPFVTSGVRIGTAAVTSRGFGLEDMDEIASLIAYTLKNHENEAALEEARKR
VEALTSKFPMYTDL

Sequences:

>Translated_414_residues
MVDHLKRQDEKVFAAIEAELGRQRSKIELIASENFVSEAVMEAQGSVLTNKYAEGYPGKRYYGGCEHVDVVEDIARDRVK
EIFGAEHVNVQPHSGAQANMAVYFTILEQGDTVLGMNLSHGGHLTHGSPVNFSGVQYNFVEYGVDAESHCINYDDVLAKA
KEHKPKLIVAGASAYPRVIDFKRFREIADEVGAYLMVDMAHIAGLVAAGLHPNPVPHAHFVTTTTHKTLRGPRGGMILCE
EQFAKQIDKSIFPGIQGGPLMHVIAAKAVAFGEALQDDFKTYAQNIINNANRLAEGLQKEGLTLVSGGTDNHLILIDVRN
LEITGKVAEHVLDEVGITVNKNTIPFETASPFVTSGVRIGTAAVTSRGFGLEDMDEIASLIAYTLKNHENEAALEEARKR
VEALTSKFPMYTDL
>Mature_414_residues
MVDHLKRQDEKVFAAIEAELGRQRSKIELIASENFVSEAVMEAQGSVLTNKYAEGYPGKRYYGGCEHVDVVEDIARDRVK
EIFGAEHVNVQPHSGAQANMAVYFTILEQGDTVLGMNLSHGGHLTHGSPVNFSGVQYNFVEYGVDAESHCINYDDVLAKA
KEHKPKLIVAGASAYPRVIDFKRFREIADEVGAYLMVDMAHIAGLVAAGLHPNPVPHAHFVTTTTHKTLRGPRGGMILCE
EQFAKQIDKSIFPGIQGGPLMHVIAAKAVAFGEALQDDFKTYAQNIINNANRLAEGLQKEGLTLVSGGTDNHLILIDVRN
LEITGKVAEHVLDEVGITVNKNTIPFETASPFVTSGVRIGTAAVTSRGFGLEDMDEIASLIAYTLKNHENEAALEEARKR
VEALTSKFPMYTDL

Specific function: Interconversion of serine and glycine [H]

COG id: COG0112

COG function: function code E; Glycine/serine hydroxymethyltransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the SHMT family [H]

Homologues:

Organism=Homo sapiens, GI19923315, Length=453, Percent_Identity=41.0596026490066, Blast_Score=343, Evalue=2e-94,
Organism=Homo sapiens, GI261862352, Length=453, Percent_Identity=41.0596026490066, Blast_Score=342, Evalue=3e-94,
Organism=Homo sapiens, GI261862350, Length=453, Percent_Identity=41.0596026490066, Blast_Score=342, Evalue=3e-94,
Organism=Homo sapiens, GI261862348, Length=453, Percent_Identity=41.0596026490066, Blast_Score=342, Evalue=3e-94,
Organism=Homo sapiens, GI261862346, Length=448, Percent_Identity=41.5178571428571, Blast_Score=332, Evalue=5e-91,
Organism=Homo sapiens, GI22547186, Length=457, Percent_Identity=41.7943107221007, Blast_Score=322, Evalue=5e-88,
Organism=Homo sapiens, GI22547189, Length=442, Percent_Identity=40.9502262443439, Blast_Score=294, Evalue=1e-79,
Organism=Escherichia coli, GI1788902, Length=417, Percent_Identity=57.7937649880096, Blast_Score=477, Evalue=1e-136,
Organism=Caenorhabditis elegans, GI25144732, Length=453, Percent_Identity=42.6048565121413, Blast_Score=356, Evalue=1e-98,
Organism=Caenorhabditis elegans, GI25144729, Length=453, Percent_Identity=42.6048565121413, Blast_Score=356, Evalue=2e-98,
Organism=Saccharomyces cerevisiae, GI6319739, Length=422, Percent_Identity=45.7345971563981, Blast_Score=362, Evalue=1e-101,
Organism=Saccharomyces cerevisiae, GI6323087, Length=405, Percent_Identity=43.7037037037037, Blast_Score=322, Evalue=7e-89,
Organism=Drosophila melanogaster, GI24640005, Length=452, Percent_Identity=42.0353982300885, Blast_Score=363, Evalue=1e-101,
Organism=Drosophila melanogaster, GI221329721, Length=452, Percent_Identity=42.0353982300885, Blast_Score=362, Evalue=1e-100,

Paralogues:

None

Copy number: 3180 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 300 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 12,000 Molecules/Cell In: Glucose minimal

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422
- InterPro:   IPR001085
- InterPro:   IPR019798 [H]

Pfam domain/function: PF00464 SHMT [H]

EC number: =2.1.2.1 [H]

Molecular weight: Translated: 45211; Mature: 45211

Theoretical pI: Translated: 5.79; Mature: 5.79

Prosite motif: PS00096 SHMT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVDHLKRQDEKVFAAIEAELGRQRSKIELIASENFVSEAVMEAQGSVLTNKYAEGYPGKR
CCCCHHHHHHHHHHHHHHHHCCCCCCEEEEECCHHHHHHHHHHCCCHHHHHHHCCCCCCC
YYGGCEHVDVVEDIARDRVKEIFGAEHVNVQPHSGAQANMAVYFTILEQGDTVLGMNLSH
CCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEEEEECCCEEEEEEECC
GGHLTHGSPVNFSGVQYNFVEYGVDAESHCINYDDVLAKAKEHKPKLIVAGASAYPRVID
CCCCCCCCCCCCCCEEEEHEEECCCCHHHCCCHHHHHHHHHHCCCCEEEECCCCCCHHHH
FKRFREIADEVGAYLMVDMAHIAGLVAAGLHPNPVPHAHFVTTTTHKTLRGPRGGMILCE
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCHHHHCCCCCCEEEEH
EQFAKQIDKSIFPGIQGGPLMHVIAAKAVAFGEALQDDFKTYAQNIINNANRLAEGLQKE
HHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GLTLVSGGTDNHLILIDVRNLEITGKVAEHVLDEVGITVNKNTIPFETASPFVTSGVRIG
CCEEEECCCCCEEEEEEECCEEECHHHHHHHHHHCCCEECCCCCCCCCCCCHHHCCEEEC
TAAVTSRGFGLEDMDEIASLIAYTLKNHENEAALEEARKRVEALTSKFPMYTDL
HHHHHCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MVDHLKRQDEKVFAAIEAELGRQRSKIELIASENFVSEAVMEAQGSVLTNKYAEGYPGKR
CCCCHHHHHHHHHHHHHHHHCCCCCCEEEEECCHHHHHHHHHHCCCHHHHHHHCCCCCCC
YYGGCEHVDVVEDIARDRVKEIFGAEHVNVQPHSGAQANMAVYFTILEQGDTVLGMNLSH
CCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEEEEECCCEEEEEEECC
GGHLTHGSPVNFSGVQYNFVEYGVDAESHCINYDDVLAKAKEHKPKLIVAGASAYPRVID
CCCCCCCCCCCCCCEEEEHEEECCCCHHHCCCHHHHHHHHHHCCCCEEEECCCCCCHHHH
FKRFREIADEVGAYLMVDMAHIAGLVAAGLHPNPVPHAHFVTTTTHKTLRGPRGGMILCE
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCHHHHCCCCCCEEEEH
EQFAKQIDKSIFPGIQGGPLMHVIAAKAVAFGEALQDDFKTYAQNIINNANRLAEGLQKE
HHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GLTLVSGGTDNHLILIDVRNLEITGKVAEHVLDEVGITVNKNTIPFETASPFVTSGVRIG
CCEEEECCCCCEEEEEEECCEEECHHHHHHHHHHCCCEECCCCCCCCCCCCHHHCCEEEC
TAAVTSRGFGLEDMDEIASLIAYTLKNHENEAALEEARKRVEALTSKFPMYTDL
HHHHHCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA