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The map label for this gene is wcaI [H]

Identifier: 49188113

GI number: 49188113

Start: 5011857

End: 5013098

Strand: Reverse

Name: wcaI [H]

Synonym: BAS5127

Alternate gene names: 49188113

Gene position: 5013098-5011857 (Counterclockwise)

Preceding gene: 49188114

Following gene: 49188112

Centisome position: 95.88

GC content: 31.32

Gene sequence:

>1242_bases
ATGAAAAAAATTTTAATGGTAACACAAAATTTTTATCCAGAAATCGGTAGTGCCTCTAATAGGATGAAAAATATATACTT
GGAATTAAATGAACGAGGTTATGATGTCAAGATATTAACTTCTGATCCTAGTTATCCAAATCGTAATTTATACAAAGATT
CGAGTTATTGGTATGATGAAAATATAGAAAAAGATATAGTACGTATCCATCCAAAAACACGAAAATATACAAGAAATCTT
TTTCGAAGGTTAATTTTGTACATAGAAGTTGCCTTTAGGTTAATTTTGGCAATATGTAAAGATAAAGAGAAATATGATTA
TATATTTGTTTCCACACCATCCATCTTTATACCTGTAGCAGGGATGTTTGCGAAACGAAAAATGAAGGCGAAATTAATTG
TAGATGTAAGGGATTTATGGCCGGAGTCCTTAATAGGCATAGGTTTTTTTAATAAAAATTGGATACTAAAATTTGCTTAT
AAATTAGAGTATAAAATATATCATGCTGCCGATAACATTATTATTAATAGTAAAGGTTTTTATTCTTATATTTCCTCTAC
AGGTATTGCCCCTAATCGCATTTCATTTATGCCTAATTCTTTAACGGAAAAAGAATTATCTACTGTTCCAAAGAAGAACA
TTAGTGATCAATTAACAGTGATTTATACAGGGAATATAGGATTAGCGCAAGATATAGAAAAATTAATTTTGATTGCAGAG
CATTTGAAAGAGTATAAGAATATTTCATTTAAGATTATTGGATATGGTTATCAAAAGAAAGAGCTTGGAGAGAGTATTGA
AGCAAAGCAACTTCCAAATATGCAATTGATTGAGCCCAAAAATCGTGAAGATACCCTCGCTGAAATTGTTGATGCAGATA
TAGCATATGTGAGTTTAGTAGAAAAAGATGTATTTAAAAAGGTTCTCCCAAGGAAAGTAATGGATTATATGAGTATGAGA
AAACCGATTGTTGCAGATGTTGCTGGTTATGCAAAAGAGGTTATTGAAGACGCTCAGTGTGGTTTTGTCACTGAAGATAG
AACGGTTGCTGAACTAAGCGATTACATTATCAAGTTAGCACAGGATAAGCAGTTACGAAATCGACTAGGGGAGAATGGAT
ATCAATATGCATTTCGAACATTACGCTGGAAAACAAATATTGAAACTTTATTGAAAATATTGGAGGAAAGAGATGTCACA
GAAGAAAGTGTGCATGTTTGTATGGAATCACTTCACAAATGA

Upstream 100 bases:

>100_bases
CTTACATTTAGATTTTTTGAGAATATTTGATTTGAAGATTTAAATAACAATGATTGTTTAAGTAATACTAACATCATAAG
TGAGCGGAGATTAATTTAGT

Downstream 100 bases:

>100_bases
TGCACGTGTATTAAGAGAATGTACAGCCTTAGCAGAATCTGGATATGAGGTTGATTTAATTTGTATTCACGATTGGAAAC
AAGAGGATCTTCCTTTATGG

Product: glycosyl transferase, group 1 family protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 413; Mature: 413

Protein sequence:

>413_residues
MKKILMVTQNFYPEIGSASNRMKNIYLELNERGYDVKILTSDPSYPNRNLYKDSSYWYDENIEKDIVRIHPKTRKYTRNL
FRRLILYIEVAFRLILAICKDKEKYDYIFVSTPSIFIPVAGMFAKRKMKAKLIVDVRDLWPESLIGIGFFNKNWILKFAY
KLEYKIYHAADNIIINSKGFYSYISSTGIAPNRISFMPNSLTEKELSTVPKKNISDQLTVIYTGNIGLAQDIEKLILIAE
HLKEYKNISFKIIGYGYQKKELGESIEAKQLPNMQLIEPKNREDTLAEIVDADIAYVSLVEKDVFKKVLPRKVMDYMSMR
KPIVADVAGYAKEVIEDAQCGFVTEDRTVAELSDYIIKLAQDKQLRNRLGENGYQYAFRTLRWKTNIETLLKILEERDVT
EESVHVCMESLHK

Sequences:

>Translated_413_residues
MKKILMVTQNFYPEIGSASNRMKNIYLELNERGYDVKILTSDPSYPNRNLYKDSSYWYDENIEKDIVRIHPKTRKYTRNL
FRRLILYIEVAFRLILAICKDKEKYDYIFVSTPSIFIPVAGMFAKRKMKAKLIVDVRDLWPESLIGIGFFNKNWILKFAY
KLEYKIYHAADNIIINSKGFYSYISSTGIAPNRISFMPNSLTEKELSTVPKKNISDQLTVIYTGNIGLAQDIEKLILIAE
HLKEYKNISFKIIGYGYQKKELGESIEAKQLPNMQLIEPKNREDTLAEIVDADIAYVSLVEKDVFKKVLPRKVMDYMSMR
KPIVADVAGYAKEVIEDAQCGFVTEDRTVAELSDYIIKLAQDKQLRNRLGENGYQYAFRTLRWKTNIETLLKILEERDVT
EESVHVCMESLHK
>Mature_413_residues
MKKILMVTQNFYPEIGSASNRMKNIYLELNERGYDVKILTSDPSYPNRNLYKDSSYWYDENIEKDIVRIHPKTRKYTRNL
FRRLILYIEVAFRLILAICKDKEKYDYIFVSTPSIFIPVAGMFAKRKMKAKLIVDVRDLWPESLIGIGFFNKNWILKFAY
KLEYKIYHAADNIIINSKGFYSYISSTGIAPNRISFMPNSLTEKELSTVPKKNISDQLTVIYTGNIGLAQDIEKLILIAE
HLKEYKNISFKIIGYGYQKKELGESIEAKQLPNMQLIEPKNREDTLAEIVDADIAYVSLVEKDVFKKVLPRKVMDYMSMR
KPIVADVAGYAKEVIEDAQCGFVTEDRTVAELSDYIIKLAQDKQLRNRLGENGYQYAFRTLRWKTNIETLLKILEERDVT
EESVHVCMESLHK

Specific function: Slime polysaccharide colanic acid biosynthesis. [C]

COG id: COG0438

COG function: function code M; Glycosyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1788363, Length=332, Percent_Identity=21.3855421686747, Blast_Score=71, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001296 [H]

Pfam domain/function: PF00534 Glycos_transf_1 [H]

EC number: NA

Molecular weight: Translated: 48147; Mature: 48147

Theoretical pI: Translated: 9.27; Mature: 9.27

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKILMVTQNFYPEIGSASNRMKNIYLELNERGYDVKILTSDPSYPNRNLYKDSSYWYDE
CCCEEEEECCCCCCCCCCCCHHEEEEEEECCCCCEEEEEECCCCCCCCCCCCCCCCCCCC
NIEKDIVRIHPKTRKYTRNLFRRLILYIEVAFRLILAICKDKEKYDYIFVSTPSIFIPVA
CCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCEEHHH
GMFAKRKMKAKLIVDVRDLWPESLIGIGFFNKNWILKFAYKLEYKIYHAADNIIINSKGF
HHHHHHHHHHHEEEEHHHCCHHHHHCCEEECCCEEEEEEEEEEEEEEEECCCEEEECCCH
YSYISSTGIAPNRISFMPNSLTEKELSTVPKKNISDQLTVIYTGNIGLAQDIEKLILIAE
HHHHHHCCCCCCCEECCCCCCCHHHHHHCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHH
HLKEYKNISFKIIGYGYQKKELGESIEAKQLPNMQLIEPKNREDTLAEIVDADIAYVSLV
HHHHHHCCEEEEEECCCCHHHHCCCHHHHHCCCCEEECCCCCHHHHHHHHHHHHHHHHHH
EKDVFKKVLPRKVMDYMSMRKPIVADVAGYAKEVIEDAQCGFVTEDRTVAELSDYIIKLA
HHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHH
QDKQLRNRLGENGYQYAFRTLRWKTNIETLLKILEERDVTEESVHVCMESLHK
CCHHHHHHHCCCHHHHHHHHHEECCCHHHHHHHHHHCCCCHHHHHHHHHHHCC
>Mature Secondary Structure
MKKILMVTQNFYPEIGSASNRMKNIYLELNERGYDVKILTSDPSYPNRNLYKDSSYWYDE
CCCEEEEECCCCCCCCCCCCHHEEEEEEECCCCCEEEEEECCCCCCCCCCCCCCCCCCCC
NIEKDIVRIHPKTRKYTRNLFRRLILYIEVAFRLILAICKDKEKYDYIFVSTPSIFIPVA
CCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCEEHHH
GMFAKRKMKAKLIVDVRDLWPESLIGIGFFNKNWILKFAYKLEYKIYHAADNIIINSKGF
HHHHHHHHHHHEEEEHHHCCHHHHHCCEEECCCEEEEEEEEEEEEEEEECCCEEEECCCH
YSYISSTGIAPNRISFMPNSLTEKELSTVPKKNISDQLTVIYTGNIGLAQDIEKLILIAE
HHHHHHCCCCCCCEECCCCCCCHHHHHHCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHH
HLKEYKNISFKIIGYGYQKKELGESIEAKQLPNMQLIEPKNREDTLAEIVDADIAYVSLV
HHHHHHCCEEEEEECCCCHHHHCCCHHHHHCCCCEEECCCCCHHHHHHHHHHHHHHHHHH
EKDVFKKVLPRKVMDYMSMRKPIVADVAGYAKEVIEDAQCGFVTEDRTVAELSDYIIKLA
HHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHH
QDKQLRNRLGENGYQYAFRTLRWKTNIETLLKILEERDVTEESVHVCMESLHK
CCHHHHHHHCCCHHHHHHHHHEECCCHHHHHHHHHHCCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7815923; 8759852; 9097040; 9278503 [H]