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The map label for this gene is ppaX

Identifier: 49187996

GI number: 49187996

Start: 4881949

End: 4882599

Strand: Reverse

Name: ppaX

Synonym: BAS5010

Alternate gene names: 49187996

Gene position: 4882599-4881949 (Counterclockwise)

Preceding gene: 49187997

Following gene: 49187995

Centisome position: 93.38

GC content: 34.25

Gene sequence:

>651_bases
ATGAAAATAAATACAGTGTTATTTGATTTAGATGGAACGTTAATTAATACAAACGAACTTATTATTTCTTCTTTTTTACA
TACTTTACATACATATTATCCAAATCAATATAAGCGTGAAGATGTATTGCCTTTTATCGGTCCATCTTTACATGATACTT
TCAGTAAGATTGATGAAAGTAAGGTTGAAGAGTTAATTACGAGTTATCGTCAATTTAACCATGATCATCATGATGAATTA
GTAGAAGAATATGAAACTGTATATGAAACAGTTCAAGAGTTAAAGAAGCAAGGCTATAAGGTTGGTATTGTTACAACGAA
AGCACGACAAACTGTTGAGATGGGATTGAAGTTATCAAAGCTTGATGAGTTTTTTGATGTTGTCGTGACAATTGATGATG
TAGAGCATGTGAAACCACATCCAGAGCCGCTTCAAAAAGCGCTTCAGTTATTAGATGCGAAACCAGAAGAAGCATTGATG
GTTGGGGATAATCATCATGATATTGTTGGTGGACAAAATGCGGGTACGAAAACAGCGGCGGTTTCATGGACATTGAAAGG
TAGAGCGTATTTAGAAACTTACAAGCCAGACTTTATGCTAGATAAAATGAGTGATTTATTGCTGATTTTGTCCGACATGA
ATCGTTCGTAA

Upstream 100 bases:

>100_bases
GGGCAAGCTGATAAAAGATATTCGGAAAATTAGAGACAAGTAGCATCTTATTATAAGATGCTGCTTCTTTCATATTCAGG
AAAGAGAAAGGACTAAGGCA

Downstream 100 bases:

>100_bases
AGATAAGTAGAGGAGCGATTTAAGTGCGACGGACAACGCGCTATCCTGTTTCAGGAGAAAGTTCACTATGGAATGTGTAT
AAAACAGTGTCTTTTTGGAA

Product: pyrophosphatase PpaX

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 216; Mature: 216

Protein sequence:

>216_residues
MKINTVLFDLDGTLINTNELIISSFLHTLHTYYPNQYKREDVLPFIGPSLHDTFSKIDESKVEELITSYRQFNHDHHDEL
VEEYETVYETVQELKKQGYKVGIVTTKARQTVEMGLKLSKLDEFFDVVVTIDDVEHVKPHPEPLQKALQLLDAKPEEALM
VGDNHHDIVGGQNAGTKTAAVSWTLKGRAYLETYKPDFMLDKMSDLLLILSDMNRS

Sequences:

>Translated_216_residues
MKINTVLFDLDGTLINTNELIISSFLHTLHTYYPNQYKREDVLPFIGPSLHDTFSKIDESKVEELITSYRQFNHDHHDEL
VEEYETVYETVQELKKQGYKVGIVTTKARQTVEMGLKLSKLDEFFDVVVTIDDVEHVKPHPEPLQKALQLLDAKPEEALM
VGDNHHDIVGGQNAGTKTAAVSWTLKGRAYLETYKPDFMLDKMSDLLLILSDMNRS
>Mature_216_residues
MKINTVLFDLDGTLINTNELIISSFLHTLHTYYPNQYKREDVLPFIGPSLHDTFSKIDESKVEELITSYRQFNHDHHDEL
VEEYETVYETVQELKKQGYKVGIVTTKARQTVEMGLKLSKLDEFFDVVVTIDDVEHVKPHPEPLQKALQLLDAKPEEALM
VGDNHHDIVGGQNAGTKTAAVSWTLKGRAYLETYKPDFMLDKMSDLLLILSDMNRS

Specific function: Hydrolyzes pyrophosphate formed during P-Ser-HPr dephosphorylation by HPrK/P. Might play a role in controlling the intracellular pyrophosphate pool

COG id: COG0546

COG function: function code R; Predicted phosphatases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HAD-like hydrolase superfamily. PpaX family

Homologues:

Organism=Escherichia coli, GI1789787, Length=234, Percent_Identity=25.6410256410256, Blast_Score=83, Evalue=1e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PPAX_BACAA (C3P0C8)

Other databases:

- EMBL:   CP001598
- RefSeq:   YP_002869380.1
- ProteinModelPortal:   C3P0C8
- SMR:   C3P0C8
- EnsemblBacteria:   EBBACT00000126563
- GeneID:   7853077
- GenomeReviews:   CP001598_GR
- KEGG:   bai:BAA_5420
- GeneTree:   EBGT00050000000474
- ProtClustDB:   PRK13288
- HAMAP:   MF_01250
- InterPro:   IPR005834
- InterPro:   IPR023214
- InterPro:   IPR006439
- InterPro:   IPR006402
- InterPro:   IPR005833
- Gene3D:   G3DSA:3.40.50.1000
- PRINTS:   PR00413
- TIGRFAMs:   TIGR01549
- TIGRFAMs:   TIGR01509

Pfam domain/function: PF00702 Hydrolase; SSF56784 SSF56784

EC number: =3.6.1.1

Molecular weight: Translated: 24767; Mature: 24767

Theoretical pI: Translated: 4.89; Mature: 4.89

Prosite motif: NA

Important sites: ACT_SITE 9-9

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKINTVLFDLDGTLINTNELIISSFLHTLHTYYPNQYKREDVLPFIGPSLHDTFSKIDES
CCCEEEEEECCCCEECCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHH
KVEELITSYRQFNHDHHDELVEEYETVYETVQELKKQGYKVGIVTTKARQTVEMGLKLSK
HHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEHHHHHHHHHCCHHHH
LDEFFDVVVTIDDVEHVKPHPEPLQKALQLLDAKPEEALMVGDNHHDIVGGQNAGTKTAA
HHHHHHHHHEECCHHHCCCCCHHHHHHHHHHCCCCCCEEEECCCCCCCCCCCCCCCCEEE
VSWTLKGRAYLETYKPDFMLDKMSDLLLILSDMNRS
EEEEECCCEEEECCCCCHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MKINTVLFDLDGTLINTNELIISSFLHTLHTYYPNQYKREDVLPFIGPSLHDTFSKIDES
CCCEEEEEECCCCEECCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHH
KVEELITSYRQFNHDHHDELVEEYETVYETVQELKKQGYKVGIVTTKARQTVEMGLKLSK
HHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEHHHHHHHHHCCHHHH
LDEFFDVVVTIDDVEHVKPHPEPLQKALQLLDAKPEEALMVGDNHHDIVGGQNAGTKTAA
HHHHHHHHHEECCHHHCCCCCHHHHHHHHHHCCCCCCEEEECCCCCCCCCCCCCCCCEEE
VSWTLKGRAYLETYKPDFMLDKMSDLLLILSDMNRS
EEEEECCCEEEECCCCCHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA