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The map label for this gene is sigL [H]

Identifier: 49187978

GI number: 49187978

Start: 4867136

End: 4868443

Strand: Reverse

Name: sigL [H]

Synonym: BAS4992

Alternate gene names: 49187978

Gene position: 4868443-4867136 (Counterclockwise)

Preceding gene: 49187987

Following gene: 49187977

Centisome position: 93.11

GC content: 38.3

Gene sequence:

>1308_bases
TTGAAGGCAAGTCTTTTACAAGAACAAAGCTTACGTTTGGCAATGACACAAGAGTTAAGGCAAGCGATTACAATGCTCCA
GTATAATGTACAAGAATTATCGGAGTTTTTGTATGAGCAATCGTTAGAGAATCCCCTTATTGAGTTAGGTGGTTTTGAGA
GGGAGAAGAAAAAGAGCTCTAACACAAGTAAAAGTACCAGCAAACAAGTCGAGAATCAGATGGAAATCTACAGTGTGGAT
TCAACAACGATTCAGCAACATTTATTAAATCAAATACAGTATTATAAAATAGAGGAAGAAGAGCGAAAAGTAGCTTCTTT
CATTATTATGAACATGGATGGAAATGGGTATTTACAAGAAACGAATGAAGAGTTAGCAAATCTTCTTTCCGCACCACTGC
ATGTAGTCGACCGCTCTGTAGAGCTTGTCCAATCACTCGAGCCAGCAGGGGTAGGGGCGCGGAATATTAAGGAATGTCTA
ACCTTGCAATTGAAGCGCTTACAAAGGCGGAATGGATTGGCAGAAGAGGTTGTAGAAGATCATTTCGCTTATTTCGTGAA
GAAAGATTGGAGAAAGCTTGTTCAAGTGTTGAAGTGTAGTAATGAAGAATTACAGGATGCAGTAAATTGTATAACGTCAT
TACAACCAAAACCAGGTCTTGCGTTTTCTTCTGATAAACCACTTTATATTGTGCCTGATATGGCAGTGAAAAAAGATGGC
GATCGTCTCGTTTTACAAATGAATGAGAGAAATATGCCGAGAATTGAGATTCATTCTGAATATAGTGCGCTTCTTAATAA
TAGTGAAAGTGAAGTAGCATCTTACGTATCAGAAAAGTATCAGCATGTGCAGTGGATTATGCGTAGTTTGAAGCAGAGAA
AACAAACCCTTCTGCAAGTGATGACCATTATTATGGAAAAACAACGTGATTTCTTCTGGAAAGGTCCGGATTATTTAAAG
CCACTTGCTTTAAAAGAAGTAGCAGAAGAGCTAAGTGTGCATGAATCTACAATTAGTCGTGCAACGCGAAATAAATATGT
GCAAACACCACACGGTTTATTTGAGATGAAATCGTTCTTTAGTAATGCCGTTTCGACGACTGAAGATGAAGCGGTTTCTA
CAAAGCGTGTAAAACAATTTATTCAAACGCTTGTTGAGGCGGAGAATAAGAAAAAGCCACTTTCAGACCAAAAGATTTCA
AAATTGTTAGAAGAAGAACATGAAATTGTTATTTCTCGAAGAACAGTGGCGAAGTATAGAGAGCAAATGCACATCCCGGC
GTCGTCATTACGAAAAACGATCGGATAG

Upstream 100 bases:

>100_bases
ATTAATACATATAAATTAATAATATTTTGGAATGGGTGTCTGTGATAGGATTCTCCTTTTACCAAACTTATTGTACAATA
GAAACAAGGGGGAGATTAGT

Downstream 100 bases:

>100_bases
GTGAAGAAGATGAAAGTTGTACTGTATACAAAAAAAGATTGTGGCCTTTGCGTGAAGGCGAAACAAGTTTTACAGGAAGT
GCAATGTGAATATTCTTTTC

Product: RNA polymerase factor sigma-54

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 435; Mature: 435

Protein sequence:

>435_residues
MKASLLQEQSLRLAMTQELRQAITMLQYNVQELSEFLYEQSLENPLIELGGFEREKKKSSNTSKSTSKQVENQMEIYSVD
STTIQQHLLNQIQYYKIEEEERKVASFIIMNMDGNGYLQETNEELANLLSAPLHVVDRSVELVQSLEPAGVGARNIKECL
TLQLKRLQRRNGLAEEVVEDHFAYFVKKDWRKLVQVLKCSNEELQDAVNCITSLQPKPGLAFSSDKPLYIVPDMAVKKDG
DRLVLQMNERNMPRIEIHSEYSALLNNSESEVASYVSEKYQHVQWIMRSLKQRKQTLLQVMTIIMEKQRDFFWKGPDYLK
PLALKEVAEELSVHESTISRATRNKYVQTPHGLFEMKSFFSNAVSTTEDEAVSTKRVKQFIQTLVEAENKKKPLSDQKIS
KLLEEEHEIVISRRTVAKYREQMHIPASSLRKTIG

Sequences:

>Translated_435_residues
MKASLLQEQSLRLAMTQELRQAITMLQYNVQELSEFLYEQSLENPLIELGGFEREKKKSSNTSKSTSKQVENQMEIYSVD
STTIQQHLLNQIQYYKIEEEERKVASFIIMNMDGNGYLQETNEELANLLSAPLHVVDRSVELVQSLEPAGVGARNIKECL
TLQLKRLQRRNGLAEEVVEDHFAYFVKKDWRKLVQVLKCSNEELQDAVNCITSLQPKPGLAFSSDKPLYIVPDMAVKKDG
DRLVLQMNERNMPRIEIHSEYSALLNNSESEVASYVSEKYQHVQWIMRSLKQRKQTLLQVMTIIMEKQRDFFWKGPDYLK
PLALKEVAEELSVHESTISRATRNKYVQTPHGLFEMKSFFSNAVSTTEDEAVSTKRVKQFIQTLVEAENKKKPLSDQKIS
KLLEEEHEIVISRRTVAKYREQMHIPASSLRKTIG
>Mature_435_residues
MKASLLQEQSLRLAMTQELRQAITMLQYNVQELSEFLYEQSLENPLIELGGFEREKKKSSNTSKSTSKQVENQMEIYSVD
STTIQQHLLNQIQYYKIEEEERKVASFIIMNMDGNGYLQETNEELANLLSAPLHVVDRSVELVQSLEPAGVGARNIKECL
TLQLKRLQRRNGLAEEVVEDHFAYFVKKDWRKLVQVLKCSNEELQDAVNCITSLQPKPGLAFSSDKPLYIVPDMAVKKDG
DRLVLQMNERNMPRIEIHSEYSALLNNSESEVASYVSEKYQHVQWIMRSLKQRKQTLLQVMTIIMEKQRDFFWKGPDYLK
PLALKEVAEELSVHESTISRATRNKYVQTPHGLFEMKSFFSNAVSTTEDEAVSTKRVKQFIQTLVEAENKKKPLSDQKIS
KLLEEEHEIVISRRTVAKYREQMHIPASSLRKTIG

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor is responsible for the expression of the levanase operon. The open complex (sigma-54 and core RNA poly

COG id: COG1508

COG function: function code K; DNA-directed RNA polymerase specialized sigma subunit, sigma54 homolog

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-54 factor family [H]

Homologues:

Organism=Escherichia coli, GI1789594, Length=476, Percent_Identity=32.3529411764706, Blast_Score=269, Evalue=3e-73,

Paralogues:

None

Copy number: 70 (log & stationary phase) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000394
- InterPro:   IPR007046
- InterPro:   IPR007634 [H]

Pfam domain/function: PF00309 Sigma54_AID; PF04963 Sigma54_CBD; PF04552 Sigma54_DBD [H]

EC number: NA

Molecular weight: Translated: 50297; Mature: 50297

Theoretical pI: Translated: 7.02; Mature: 7.02

Prosite motif: PS00717 SIGMA54_1 ; PS00718 SIGMA54_2 ; PS50044 SIGMA54_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKASLLQEQSLRLAMTQELRQAITMLQYNVQELSEFLYEQSLENPLIELGGFEREKKKSS
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCHHHHHCC
NTSKSTSKQVENQMEIYSVDSTTIQQHLLNQIQYYKIEEEERKVASFIIMNMDGNGYLQE
CCCHHHHHHHHHHHHHEECCHHHHHHHHHHHHHHHCCCHHHHHHHHHHEEECCCCCCHHH
TNEELANLLSAPLHVVDRSVELVQSLEPAGVGARNIKECLTLQLKRLQRRNGLAEEVVED
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHH
HFAYFVKKDWRKLVQVLKCSNEELQDAVNCITSLQPKPGLAFSSDKPLYIVPDMAVKKDG
HHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCCCCCCEEEECCHHHCCCC
DRLVLQMNERNMPRIEIHSEYSALLNNSESEVASYVSEKYQHVQWIMRSLKQRKQTLLQV
CEEEEEECCCCCCEEEEHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MTIIMEKQRDFFWKGPDYLKPLALKEVAEELSVHESTISRATRNKYVQTPHGLFEMKSFF
HHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHH
SNAVSTTEDEAVSTKRVKQFIQTLVEAENKKKPLSDQKISKLLEEEHEIVISRRTVAKYR
HHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
EQMHIPASSLRKTIG
HHHCCCHHHHHHHCC
>Mature Secondary Structure
MKASLLQEQSLRLAMTQELRQAITMLQYNVQELSEFLYEQSLENPLIELGGFEREKKKSS
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCHHHHHCC
NTSKSTSKQVENQMEIYSVDSTTIQQHLLNQIQYYKIEEEERKVASFIIMNMDGNGYLQE
CCCHHHHHHHHHHHHHEECCHHHHHHHHHHHHHHHCCCHHHHHHHHHHEEECCCCCCHHH
TNEELANLLSAPLHVVDRSVELVQSLEPAGVGARNIKECLTLQLKRLQRRNGLAEEVVED
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHH
HFAYFVKKDWRKLVQVLKCSNEELQDAVNCITSLQPKPGLAFSSDKPLYIVPDMAVKKDG
HHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCCCCCCEEEECCHHHCCCC
DRLVLQMNERNMPRIEIHSEYSALLNNSESEVASYVSEKYQHVQWIMRSLKQRKQTLLQV
CEEEEEECCCCCCEEEEHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MTIIMEKQRDFFWKGPDYLKPLALKEVAEELSVHESTISRATRNKYVQTPHGLFEMKSFF
HHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHH
SNAVSTTEDEAVSTKRVKQFIQTLVEAENKKKPLSDQKISKLLEEEHEIVISRRTVAKYR
HHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
EQMHIPASSLRKTIG
HHHCCCHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1924373; 8969506; 9384377 [H]