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The map label for this gene is eno

Identifier: 49187971

GI number: 49187971

Start: 4859721

End: 4861016

Strand: Reverse

Name: eno

Synonym: BAS4985

Alternate gene names: 49187971

Gene position: 4861016-4859721 (Counterclockwise)

Preceding gene: 49187972

Following gene: 49187970

Centisome position: 92.97

GC content: 42.05

Gene sequence:

>1296_bases
ATGTCAACAATTATTGATGTTTATGCTCGCGAAGTCCTTGACTCTCGTGGTAACCCAACTGTAGAAGTAGAAGTTTACAC
AGAAAGCGGCGCTTTCGGACGCGCTATCGTACCAAGTGGTGCATCTACTGGTGAGCACGAAGCAGTAGAATTACGTGACG
GTGACAAATCTCGTTACCTTGGTAAAGGTGTTATGAACGCAGTAAACAACGTTAATGAAGCAATCGCTCCAGAAATCGTT
GGTTTCGACGTAACTGACCAAGCTGGTATCGACCGTGCTATGATCGAATTAGATGGCACTCCAAACAAAGGTAAACTAGG
CGCTAACGCTATCCTTGGTGTATCTATGGCAGTAGCTCACGCAGCAGCTGACTTCGTAGGTCTTCCATTATACCGTTACC
TTGGTGGATTCAATGCAAAACAATTACCAACTCCAATGATGAACATCATCAACGGTGGTTCTCACGCTGATAACAACGTT
GACTTCCAAGAGTTCATGATCTTACCAGTTGGTGCTCCAACATTCAAAGAATCAATCCGTATGGGTGCTGAAGTATTCCA
TGCACTTAAAGCTGTATTACATGACAAAGGTCTTAACACTGCAGTAGGTGACGAAGGTGGATTCGCTCCAAACCTTGGTT
CTAACCGTGAAGCATTAGAAGTAATCATCGAAGCTATCGAAAAAGCTGGTTACAAAGCTGGCGAGAACGTATTCTTAGGA
ATGGACGTTGCTTCTTCTGAGTTCTACAACAAAGAAACTGGTAAATATGACCTTGCAGGTGAAGGCCGTACTGGCTTAAC
TTCTGCAGAAATGGTTGATTTCTACGAAGAGCTTTGCAAAGACTTCCCAATCATCTCTATCGAAGATGGTTTAGACGAAA
ACGACTGGGATGGTCACAAATTATTAACTGAGCGTATCGGTGATAAAGTACAATTAGTTGGTGACGATTTATTCGTAACT
AACACTCAAAAACTTGCTGAAGGTATCGAAAAAGGTATCTCTAACTCAATCTTAATTAAAGTTAACCAAATCGGTACTTT
AACTGAGACTTTCGAAGCTATCGAAATGGCTAAACGTGCTGGTTACACAGCAGTTGTATCTCACCGTTCTGGTGAAACTG
AAGATGCTACAATTGCTGACATCGCAGTTGCAACTAACGCTGGCCAAATCAAAACTGGTTCTATGAGCCGTACTGACCGT
ATTGCTAAGTACAACCAATTATTACGCATCGAAGACGAACTAGGCGAAATCGCTGTTTACGATGGTATCAAATCTTTTTA
TAACATCAAACGATAA

Upstream 100 bases:

>100_bases
TCCAACAATGCTTACACTTCTTGGTGTTGAGCAACCGAAAGAAATGACAGGTAAAACAATTATTAAATAATTTGCTTATA
TAAAAAGGAGAGAATTTATT

Downstream 100 bases:

>100_bases
TTATAAAGAGAAAAAAATCGACTGAACTTTGTCATACAAAGCAATTTGCTAACGCTTGAATTGAGCAATGCGGAAGCAAA
TACTTAGTCGTACACAAACC

Product: phosphopyruvate hydratase

Products: NA

Alternate protein names: 2-phospho-D-glycerate hydro-lyase; 2-phosphoglycerate dehydratase

Number of amino acids: Translated: 431; Mature: 430

Protein sequence:

>431_residues
MSTIIDVYAREVLDSRGNPTVEVEVYTESGAFGRAIVPSGASTGEHEAVELRDGDKSRYLGKGVMNAVNNVNEAIAPEIV
GFDVTDQAGIDRAMIELDGTPNKGKLGANAILGVSMAVAHAAADFVGLPLYRYLGGFNAKQLPTPMMNIINGGSHADNNV
DFQEFMILPVGAPTFKESIRMGAEVFHALKAVLHDKGLNTAVGDEGGFAPNLGSNREALEVIIEAIEKAGYKAGENVFLG
MDVASSEFYNKETGKYDLAGEGRTGLTSAEMVDFYEELCKDFPIISIEDGLDENDWDGHKLLTERIGDKVQLVGDDLFVT
NTQKLAEGIEKGISNSILIKVNQIGTLTETFEAIEMAKRAGYTAVVSHRSGETEDATIADIAVATNAGQIKTGSMSRTDR
IAKYNQLLRIEDELGEIAVYDGIKSFYNIKR

Sequences:

>Translated_431_residues
MSTIIDVYAREVLDSRGNPTVEVEVYTESGAFGRAIVPSGASTGEHEAVELRDGDKSRYLGKGVMNAVNNVNEAIAPEIV
GFDVTDQAGIDRAMIELDGTPNKGKLGANAILGVSMAVAHAAADFVGLPLYRYLGGFNAKQLPTPMMNIINGGSHADNNV
DFQEFMILPVGAPTFKESIRMGAEVFHALKAVLHDKGLNTAVGDEGGFAPNLGSNREALEVIIEAIEKAGYKAGENVFLG
MDVASSEFYNKETGKYDLAGEGRTGLTSAEMVDFYEELCKDFPIISIEDGLDENDWDGHKLLTERIGDKVQLVGDDLFVT
NTQKLAEGIEKGISNSILIKVNQIGTLTETFEAIEMAKRAGYTAVVSHRSGETEDATIADIAVATNAGQIKTGSMSRTDR
IAKYNQLLRIEDELGEIAVYDGIKSFYNIKR
>Mature_430_residues
STIIDVYAREVLDSRGNPTVEVEVYTESGAFGRAIVPSGASTGEHEAVELRDGDKSRYLGKGVMNAVNNVNEAIAPEIVG
FDVTDQAGIDRAMIELDGTPNKGKLGANAILGVSMAVAHAAADFVGLPLYRYLGGFNAKQLPTPMMNIINGGSHADNNVD
FQEFMILPVGAPTFKESIRMGAEVFHALKAVLHDKGLNTAVGDEGGFAPNLGSNREALEVIIEAIEKAGYKAGENVFLGM
DVASSEFYNKETGKYDLAGEGRTGLTSAEMVDFYEELCKDFPIISIEDGLDENDWDGHKLLTERIGDKVQLVGDDLFVTN
TQKLAEGIEKGISNSILIKVNQIGTLTETFEAIEMAKRAGYTAVVSHRSGETEDATIADIAVATNAGQIKTGSMSRTDRI
AKYNQLLRIEDELGEIAVYDGIKSFYNIKR

Specific function: Catalyzes the reversible conversion of 2- phosphoglycerate into phosphoenolpyruvate. It is essential for the degradation of carbohydrates via glycolysis

COG id: COG0148

COG function: function code G; Enolase

Gene ontology:

Cell location: Cytoplasm. Secreted. Cell surface. Note=Fractions of enolase are present in both the cytoplasm and on the cell surface. The export of enolase possibly depends on the covalent binding to the substrate; once secreted, it remains attached to the cell surface

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enolase family

Homologues:

Organism=Homo sapiens, GI5803011, Length=431, Percent_Identity=53.3642691415313, Blast_Score=440, Evalue=1e-123,
Organism=Homo sapiens, GI4503571, Length=437, Percent_Identity=52.1739130434783, Blast_Score=434, Evalue=1e-122,
Organism=Homo sapiens, GI301897477, Length=435, Percent_Identity=52.6436781609195, Blast_Score=430, Evalue=1e-120,
Organism=Homo sapiens, GI301897469, Length=435, Percent_Identity=52.6436781609195, Blast_Score=430, Evalue=1e-120,
Organism=Homo sapiens, GI301897479, Length=433, Percent_Identity=47.8060046189376, Blast_Score=373, Evalue=1e-103,
Organism=Homo sapiens, GI169201331, Length=340, Percent_Identity=26.1764705882353, Blast_Score=100, Evalue=4e-21,
Organism=Homo sapiens, GI169201757, Length=340, Percent_Identity=26.1764705882353, Blast_Score=100, Evalue=4e-21,
Organism=Homo sapiens, GI239744207, Length=340, Percent_Identity=26.1764705882353, Blast_Score=100, Evalue=4e-21,
Organism=Escherichia coli, GI1789141, Length=428, Percent_Identity=66.8224299065421, Blast_Score=556, Evalue=1e-160,
Organism=Caenorhabditis elegans, GI71995829, Length=431, Percent_Identity=54.292343387471, Blast_Score=447, Evalue=1e-126,
Organism=Caenorhabditis elegans, GI17536383, Length=431, Percent_Identity=54.292343387471, Blast_Score=446, Evalue=1e-125,
Organism=Caenorhabditis elegans, GI32563855, Length=191, Percent_Identity=47.1204188481675, Blast_Score=180, Evalue=2e-45,
Organism=Saccharomyces cerevisiae, GI6321693, Length=430, Percent_Identity=51.1627906976744, Blast_Score=409, Evalue=1e-115,
Organism=Saccharomyces cerevisiae, GI6323985, Length=434, Percent_Identity=50.2304147465438, Blast_Score=408, Evalue=1e-115,
Organism=Saccharomyces cerevisiae, GI6324974, Length=434, Percent_Identity=50.2304147465438, Blast_Score=407, Evalue=1e-114,
Organism=Saccharomyces cerevisiae, GI6324969, Length=434, Percent_Identity=50.2304147465438, Blast_Score=407, Evalue=1e-114,
Organism=Saccharomyces cerevisiae, GI6321968, Length=430, Percent_Identity=52.093023255814, Blast_Score=382, Evalue=1e-107,
Organism=Drosophila melanogaster, GI24580918, Length=436, Percent_Identity=52.9816513761468, Blast_Score=419, Evalue=1e-117,
Organism=Drosophila melanogaster, GI24580916, Length=436, Percent_Identity=52.9816513761468, Blast_Score=419, Evalue=1e-117,
Organism=Drosophila melanogaster, GI24580920, Length=436, Percent_Identity=52.9816513761468, Blast_Score=419, Evalue=1e-117,
Organism=Drosophila melanogaster, GI24580914, Length=436, Percent_Identity=52.9816513761468, Blast_Score=419, Evalue=1e-117,
Organism=Drosophila melanogaster, GI281360527, Length=436, Percent_Identity=52.9816513761468, Blast_Score=417, Evalue=1e-117,
Organism=Drosophila melanogaster, GI17137654, Length=436, Percent_Identity=52.9816513761468, Blast_Score=417, Evalue=1e-117,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1660 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 20 Molecules/Cell In: Stationary Phase,

Swissprot (AC and ID): ENO_BACAA (C3P0A3)

Other databases:

- EMBL:   CP001598
- RefSeq:   YP_002869355.1
- ProteinModelPortal:   C3P0A3
- SMR:   C3P0A3
- EnsemblBacteria:   EBBACT00000129062
- GeneID:   7850786
- GenomeReviews:   CP001598_GR
- KEGG:   bai:BAA_5394
- GeneTree:   EBGT00050000001934
- ProtClustDB:   PRK00077
- GO:   GO:0006096
- HAMAP:   MF_00318
- InterPro:   IPR000941
- InterPro:   IPR020810
- InterPro:   IPR020809
- InterPro:   IPR020811
- PIRSF:   PIRSF001400
- PRINTS:   PR00148
- TIGRFAMs:   TIGR01060

Pfam domain/function: PF00113 Enolase_C; PF03952 Enolase_N

EC number: =4.2.1.11

Molecular weight: Translated: 46419; Mature: 46288

Theoretical pI: Translated: 4.39; Mature: 4.39

Prosite motif: PS00164 ENOLASE

Important sites: ACT_SITE 205-205 ACT_SITE 340-340 BINDING 155-155 BINDING 164-164 BINDING 288-288 BINDING 315-315 BINDING 340-340 BINDING 391-391

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTIIDVYAREVLDSRGNPTVEVEVYTESGAFGRAIVPSGASTGEHEAVELRDGDKSRYL
CCHHHHHHHHHHHHCCCCCEEEEEEEECCCCCCCEECCCCCCCCCCCEEEECCCCCHHHH
GKGVMNAVNNVNEAIAPEIVGFDVTDQAGIDRAMIELDGTPNKGKLGANAILGVSMAVAH
HHHHHHHHHHHHHHHCCHHCCCCCCCCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHH
AAADFVGLPLYRYLGGFNAKQLPTPMMNIINGGSHADNNVDFQEFMILPVGAPTFKESIR
HHHHHHHHHHHHHHCCCCCCCCCCHHHHHHCCCCCCCCCCCHHHEEEEECCCHHHHHHHH
MGAEVFHALKAVLHDKGLNTAVGDEGGFAPNLGSNREALEVIIEAIEKAGYKAGENVFLG
HHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCEEEEE
MDVASSEFYNKETGKYDLAGEGRTGLTSAEMVDFYEELCKDFPIISIEDGLDENDWDGHK
EECCCHHHCCCCCCCEECCCCCCCCCCHHHHHHHHHHHHHHCCEEEECCCCCCCCCCHHH
LLTERIGDKVQLVGDDLFVTNTQKLAEGIEKGISNSILIKVNQIGTLTETFEAIEMAKRA
HHHHHCCCEEEEECCCEEEECHHHHHHHHHHCCCCEEEEEEECCCCHHHHHHHHHHHHHC
GYTAVVSHRSGETEDATIADIAVATNAGQIKTGSMSRTDRIAKYNQLLRIEDELGEIAVY
CCEEEEECCCCCCCCCEEEEEEEECCCCCEECCCCHHHHHHHHHHHHHEEHHHHCCEEHH
DGIKSFYNIKR
HHHHHHHCCCC
>Mature Secondary Structure 
STIIDVYAREVLDSRGNPTVEVEVYTESGAFGRAIVPSGASTGEHEAVELRDGDKSRYL
CHHHHHHHHHHHHCCCCCEEEEEEEECCCCCCCEECCCCCCCCCCCEEEECCCCCHHHH
GKGVMNAVNNVNEAIAPEIVGFDVTDQAGIDRAMIELDGTPNKGKLGANAILGVSMAVAH
HHHHHHHHHHHHHHHCCHHCCCCCCCCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHH
AAADFVGLPLYRYLGGFNAKQLPTPMMNIINGGSHADNNVDFQEFMILPVGAPTFKESIR
HHHHHHHHHHHHHHCCCCCCCCCCHHHHHHCCCCCCCCCCCHHHEEEEECCCHHHHHHHH
MGAEVFHALKAVLHDKGLNTAVGDEGGFAPNLGSNREALEVIIEAIEKAGYKAGENVFLG
HHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCEEEEE
MDVASSEFYNKETGKYDLAGEGRTGLTSAEMVDFYEELCKDFPIISIEDGLDENDWDGHK
EECCCHHHCCCCCCCEECCCCCCCCCCHHHHHHHHHHHHHHCCEEEECCCCCCCCCCHHH
LLTERIGDKVQLVGDDLFVTNTQKLAEGIEKGISNSILIKVNQIGTLTETFEAIEMAKRA
HHHHHCCCEEEEECCCEEEECHHHHHHHHHHCCCCEEEEEEECCCCHHHHHHHHHHHHHC
GYTAVVSHRSGETEDATIADIAVATNAGQIKTGSMSRTDRIAKYNQLLRIEDELGEIAVY
CCEEEEECCCCCCCCCEEEEEEEECCCCCEECCCCHHHHHHHHHHHHHEEHHHHCCEEHH
DGIKSFYNIKR
HHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA