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The map label for this gene is dacF [H]

Identifier: 49187867

GI number: 49187867

Start: 4759384

End: 4760223

Strand: Direct

Name: dacF [H]

Synonym: BAS4878

Alternate gene names: 49187867

Gene position: 4759384-4760223 (Clockwise)

Preceding gene: 49187856

Following gene: 49187872

Centisome position: 91.02

GC content: 36.79

Gene sequence:

>840_bases
ATGAAACGAACAATAGTTATGATTGTAATGATTGCCACACTATTTACAGGGATGATTTTCTTCTCTTATGCACAAAGACA
ACAAGCTGTAAGAGCCGATCAGCCCAGCATCACTGGACAATACGGAATTACAATCGATGCAGACACAGGTGAAATTTTGT
ATGGAAAACGAGAAGACGAACGCTCTTATCCGGCTAGTATAGCCAAAATGATGACAACCCTTCTTTTACTAGAAAATGTA
AAAGAGGATGAAGAAATTACAGTTACAGAAAACGCAATTAAAACAGAAAGTCAAAGTAAGAAAATTAAACTTCGTGCTGG
GGAAAAGTTAAAACGTGATGAGGCATTAAAACTGATGCTTATCATTAGTGCAGATCCAATAGCTGAATCCATCGCAGAAC
ATATTGCAGGATCAAAAAATGAATTTGTAAAAATGATGAATGCTAGAGCGAAGGAACTTGGTACAAAACATGCGACTTTC
AAAAATGCAAGTGGTGCTGATGCATTAGGAAACAAAGTATCACCATATGACATTGCTATGATTACGAAAGAAGCATTAAA
ATACCCAGTTGTTTTAGAATATATGAATTCAACGCGTACAACTCTACATACTTCAGAACGCTCTCCAAACATCGCAAACT
ATGGACGAGAAGAACTATATGACGATCCATATGCAATTGGAAGTAAAAGCGGTCTATCAGCACTCGGAAAATATACAGTC
GTAACAGTGGATGAGAAAGACGGTAAACGCGTCATTAATGTTGTATTATCTTCTACTCGCAAGCAACTGTATCCTGATAC
GAAGAAAATGGCACATTACGCATTTCAGCAATTAAAATAA

Upstream 100 bases:

>100_bases
GGGCGAAAAATAACGCTATATTTTTGGGCAGAGAACGTGGCATAATGGGTAATGGCTGCTTTTTCTGCCCAATTTATTTG
TAACAAATGAGGGAATTATA

Downstream 100 bases:

>100_bases
CCAAGGAGTATTTACTCCTTGGTTATTTTTTAACCATACCTTTTAATACGAACGCAACGTTTGCTGGACGCTCTGCTAGA
CGACGCATGAAGTAGCCATA

Product: D-alanyl-D-alanine carboxypeptidase family protein

Products: NA

Alternate protein names: DD-carboxypeptidase; DD-peptidase; Penicillin-binding protein dacF; PBP [H]

Number of amino acids: Translated: 279; Mature: 279

Protein sequence:

>279_residues
MKRTIVMIVMIATLFTGMIFFSYAQRQQAVRADQPSITGQYGITIDADTGEILYGKREDERSYPASIAKMMTTLLLLENV
KEDEEITVTENAIKTESQSKKIKLRAGEKLKRDEALKLMLIISADPIAESIAEHIAGSKNEFVKMMNARAKELGTKHATF
KNASGADALGNKVSPYDIAMITKEALKYPVVLEYMNSTRTTLHTSERSPNIANYGREELYDDPYAIGSKSGLSALGKYTV
VTVDEKDGKRVINVVLSSTRKQLYPDTKKMAHYAFQQLK

Sequences:

>Translated_279_residues
MKRTIVMIVMIATLFTGMIFFSYAQRQQAVRADQPSITGQYGITIDADTGEILYGKREDERSYPASIAKMMTTLLLLENV
KEDEEITVTENAIKTESQSKKIKLRAGEKLKRDEALKLMLIISADPIAESIAEHIAGSKNEFVKMMNARAKELGTKHATF
KNASGADALGNKVSPYDIAMITKEALKYPVVLEYMNSTRTTLHTSERSPNIANYGREELYDDPYAIGSKSGLSALGKYTV
VTVDEKDGKRVINVVLSSTRKQLYPDTKKMAHYAFQQLK
>Mature_279_residues
MKRTIVMIVMIATLFTGMIFFSYAQRQQAVRADQPSITGQYGITIDADTGEILYGKREDERSYPASIAKMMTTLLLLENV
KEDEEITVTENAIKTESQSKKIKLRAGEKLKRDEALKLMLIISADPIAESIAEHIAGSKNEFVKMMNARAKELGTKHATF
KNASGADALGNKVSPYDIAMITKEALKYPVVLEYMNSTRTTLHTSERSPNIANYGREELYDDPYAIGSKSGLSALGKYTV
VTVDEKDGKRVINVVLSSTRKQLYPDTKKMAHYAFQQLK

Specific function: Removes C-terminal D-alanyl residues from sugar-peptide cell wall precursors [H]

COG id: COG1686

COG function: function code M; D-alanyl-D-alanine carboxypeptidase

Gene ontology:

Cell location: Secreted (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S11 family [H]

Homologues:

Organism=Escherichia coli, GI1787062, Length=261, Percent_Identity=27.5862068965517, Blast_Score=86, Evalue=3e-18,
Organism=Escherichia coli, GI87082022, Length=268, Percent_Identity=23.134328358209, Blast_Score=75, Evalue=3e-15,
Organism=Escherichia coli, GI1786851, Length=255, Percent_Identity=27.0588235294118, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI87082054, Length=174, Percent_Identity=27.5862068965517, Blast_Score=66, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR015956
- InterPro:   IPR018044
- InterPro:   IPR012907
- InterPro:   IPR001967 [H]

Pfam domain/function: PF07943 PBP5_C; PF00768 Peptidase_S11 [H]

EC number: =3.4.16.4 [H]

Molecular weight: Translated: 31150; Mature: 31150

Theoretical pI: Translated: 9.53; Mature: 9.53

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
4.3 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRTIVMIVMIATLFTGMIFFSYAQRQQAVRADQPSITGQYGITIDADTGEILYGKREDE
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEECCCCCEEECCCCCC
RSYPASIAKMMTTLLLLENVKEDEEITVTENAIKTESQSKKIKLRAGEKLKRDEALKLML
CCCHHHHHHHHHHHHHHHHCCCCCCEEEEHHHHHCCCCCCEEEEECCCCCCHHCCEEEEE
IISADPIAESIAEHIAGSKNEFVKMMNARAKELGTKHATFKNASGADALGNKVSPYDIAM
EEECCHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCHHCCCCCCCCCHHHCCCCCCHHHHH
ITKEALKYPVVLEYMNSTRTTLHTSERSPNIANYGREELYDDPYAIGSKSGLSALGKYTV
HHHHHHCCCHHHHHHHCCCCEEECCCCCCCHHHCCHHHHCCCCCCCCCCCCHHHHCCEEE
VTVDEKDGKRVINVVLSSTRKQLYPDTKKMAHYAFQQLK
EEEECCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCC
>Mature Secondary Structure
MKRTIVMIVMIATLFTGMIFFSYAQRQQAVRADQPSITGQYGITIDADTGEILYGKREDE
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEECCCCCEEECCCCCC
RSYPASIAKMMTTLLLLENVKEDEEITVTENAIKTESQSKKIKLRAGEKLKRDEALKLML
CCCHHHHHHHHHHHHHHHHCCCCCCEEEEHHHHHCCCCCCEEEEECCCCCCHHCCEEEEE
IISADPIAESIAEHIAGSKNEFVKMMNARAKELGTKHATFKNASGADALGNKVSPYDIAM
EEECCHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCHHCCCCCCCCCHHHCCCCCCHHHHH
ITKEALKYPVVLEYMNSTRTTLHTSERSPNIANYGREELYDDPYAIGSKSGLSALGKYTV
HHHHHHCCCHHHHHHHCCCCEEECCCCCCCHHHCCHHHHCCCCCCCCCCCCHHHHCCEEE
VTVDEKDGKRVINVVLSSTRKQLYPDTKKMAHYAFQQLK
EEEECCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1629150; 8969508; 9384377 [H]