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The map label for this gene is ldh

Identifier: 49187858

GI number: 49187858

Start: 4748776

End: 4749726

Strand: Reverse

Name: ldh

Synonym: BAS4869

Alternate gene names: 49187858

Gene position: 4749726-4748776 (Counterclockwise)

Preceding gene: 49187859

Following gene: 49187857

Centisome position: 90.84

GC content: 35.86

Gene sequence:

>951_bases
ATGAAAAGACATACAAGAAAAATTGCAATTATCGGTACTGGGTTAGTTGGATCAAGTTGTGCATATTCCATTGTCAATCA
AGGCATTTGCGAAGAGCTATTATTAATTGATATAAATCATGAACGTGCAGTTGGGGAAGCGATGGATTTATCGCACTGCA
TTAACTTTACAAATACAAGAACAAAAGTATATGCAGGAAGCTATGAAGACTGCAAAGATATGGACATCGTTATTATTACA
GCAGGACCAGCACCAAAACCAGGACAAAGTCGCTTAGATACTTTAGGAGCGAGCGCGAAGATTATGGAAAGTGTTGTTGG
CGGCGTAATGGAAAGTGGATTTGATGGTATTTTCTTACTTGCATCGAACCCAGTTGATATTATTACGTATCAAGTGTGGA
AATTATCTGGATTACCTAGAAATCGTGTGATTGGTACTGGTACATCACTAGATTCTTCTCGCTTACGAACAATTTTATCT
GAAATGTTACATGTAGACCCTCGTAGTATTCATGGGTATTCATTAGGAGAACATGGTGATTCTCAAATGGTTGCTTGGTC
ACACGTAACTGTTGGTGGAAAACCAATTCTGCAAATATTAGAAGAACAAAAAGAACGATTTGGTGAAATAGATTTAGATG
AAATCGTTGAGAAGACTGCAAAAGCTGGATGGGAAATTTATAAACGAAAAGGTACTACTTATTATGGAATTGGAAATTCT
CTAGCATATATTGCGAGCTCAATCTTTAACGATGATCACCGTGTAATTGCTGTATCAGCCATTTTAGATGGTGAGTATGG
TGAATATGATATTTGTACAGGAGTACCAGCTATTATTACTAGAGATGGTATAAGAGAAATTGTAGAACTAAATTTAACAG
AGGATGAAGAATCTCGATTCGCAAAATCAAACGATATTTTACGTGATTATATGAAAACAATTGGTTACTAA

Upstream 100 bases:

>100_bases
ATTTCACAAAGTATCCATGGTTTCTATCGTGGGAACCTCTATAATTAGGAGTGTAAGGAACAAAAGAAATAGATTATATG
ATCAATTAGGAGAGATTGCT

Downstream 100 bases:

>100_bases
CTTATAGGAGAAGGTGAAACAAATGAAGGAATATATAATGTCTCGTGTATTTAAAGCATCAGCTGGAATCGCACAAGGTA
TTTTCGTATCCCTTGGAATT

Product: L-lactate dehydrogenase

Products: NA

Alternate protein names: L-LDH 3

Number of amino acids: Translated: 316; Mature: 316

Protein sequence:

>316_residues
MKRHTRKIAIIGTGLVGSSCAYSIVNQGICEELLLIDINHERAVGEAMDLSHCINFTNTRTKVYAGSYEDCKDMDIVIIT
AGPAPKPGQSRLDTLGASAKIMESVVGGVMESGFDGIFLLASNPVDIITYQVWKLSGLPRNRVIGTGTSLDSSRLRTILS
EMLHVDPRSIHGYSLGEHGDSQMVAWSHVTVGGKPILQILEEQKERFGEIDLDEIVEKTAKAGWEIYKRKGTTYYGIGNS
LAYIASSIFNDDHRVIAVSAILDGEYGEYDICTGVPAIITRDGIREIVELNLTEDEESRFAKSNDILRDYMKTIGY

Sequences:

>Translated_316_residues
MKRHTRKIAIIGTGLVGSSCAYSIVNQGICEELLLIDINHERAVGEAMDLSHCINFTNTRTKVYAGSYEDCKDMDIVIIT
AGPAPKPGQSRLDTLGASAKIMESVVGGVMESGFDGIFLLASNPVDIITYQVWKLSGLPRNRVIGTGTSLDSSRLRTILS
EMLHVDPRSIHGYSLGEHGDSQMVAWSHVTVGGKPILQILEEQKERFGEIDLDEIVEKTAKAGWEIYKRKGTTYYGIGNS
LAYIASSIFNDDHRVIAVSAILDGEYGEYDICTGVPAIITRDGIREIVELNLTEDEESRFAKSNDILRDYMKTIGY
>Mature_316_residues
MKRHTRKIAIIGTGLVGSSCAYSIVNQGICEELLLIDINHERAVGEAMDLSHCINFTNTRTKVYAGSYEDCKDMDIVIIT
AGPAPKPGQSRLDTLGASAKIMESVVGGVMESGFDGIFLLASNPVDIITYQVWKLSGLPRNRVIGTGTSLDSSRLRTILS
EMLHVDPRSIHGYSLGEHGDSQMVAWSHVTVGGKPILQILEEQKERFGEIDLDEIVEKTAKAGWEIYKRKGTTYYGIGNS
LAYIASSIFNDDHRVIAVSAILDGEYGEYDICTGVPAIITRDGIREIVELNLTEDEESRFAKSNDILRDYMKTIGY

Specific function: Catalyzes The Reversible Oxidation Of Malate To Oxaloacetate. [C]

COG id: COG0039

COG function: function code C; Malate/lactate dehydrogenases

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the LDH/MDH superfamily. LDH family

Homologues:

Organism=Homo sapiens, GI5031857, Length=316, Percent_Identity=37.3417721518987, Blast_Score=214, Evalue=1e-55,
Organism=Homo sapiens, GI260099723, Length=316, Percent_Identity=37.3417721518987, Blast_Score=213, Evalue=2e-55,
Organism=Homo sapiens, GI47059044, Length=312, Percent_Identity=36.8589743589744, Blast_Score=207, Evalue=1e-53,
Organism=Homo sapiens, GI221136809, Length=312, Percent_Identity=36.8589743589744, Blast_Score=207, Evalue=1e-53,
Organism=Homo sapiens, GI291575128, Length=311, Percent_Identity=35.048231511254, Blast_Score=206, Evalue=2e-53,
Organism=Homo sapiens, GI4557032, Length=311, Percent_Identity=35.048231511254, Blast_Score=206, Evalue=2e-53,
Organism=Homo sapiens, GI9257228, Length=316, Percent_Identity=36.7088607594937, Blast_Score=206, Evalue=3e-53,
Organism=Homo sapiens, GI4504973, Length=316, Percent_Identity=36.7088607594937, Blast_Score=206, Evalue=3e-53,
Organism=Homo sapiens, GI15082234, Length=307, Percent_Identity=34.5276872964169, Blast_Score=201, Evalue=6e-52,
Organism=Homo sapiens, GI260099727, Length=218, Percent_Identity=38.5321100917431, Blast_Score=159, Evalue=4e-39,
Organism=Homo sapiens, GI260099725, Length=187, Percent_Identity=42.2459893048128, Blast_Score=157, Evalue=2e-38,
Organism=Homo sapiens, GI207028494, Length=234, Percent_Identity=36.7521367521368, Blast_Score=147, Evalue=1e-35,
Organism=Homo sapiens, GI103472011, Length=294, Percent_Identity=28.9115646258503, Blast_Score=121, Evalue=9e-28,
Organism=Homo sapiens, GI103472015, Length=185, Percent_Identity=31.8918918918919, Blast_Score=97, Evalue=3e-20,
Organism=Homo sapiens, GI21735621, Length=239, Percent_Identity=28.0334728033473, Blast_Score=75, Evalue=9e-14,
Organism=Escherichia coli, GI1789632, Length=296, Percent_Identity=26.6891891891892, Blast_Score=61, Evalue=9e-11,
Organism=Caenorhabditis elegans, GI17535107, Length=298, Percent_Identity=36.241610738255, Blast_Score=194, Evalue=4e-50,
Organism=Saccharomyces cerevisiae, GI6322765, Length=267, Percent_Identity=27.7153558052434, Blast_Score=64, Evalue=2e-11,
Organism=Drosophila melanogaster, GI17136226, Length=317, Percent_Identity=36.9085173501577, Blast_Score=211, Evalue=5e-55,
Organism=Drosophila melanogaster, GI45550422, Length=308, Percent_Identity=30.1948051948052, Blast_Score=167, Evalue=1e-41,
Organism=Drosophila melanogaster, GI24663599, Length=315, Percent_Identity=26.984126984127, Blast_Score=72, Evalue=6e-13,
Organism=Drosophila melanogaster, GI24647881, Length=316, Percent_Identity=25.3164556962025, Blast_Score=70, Evalue=3e-12,

Paralogues:

None

Copy number: 2640 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 260 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2380 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). 260 Molecules/Cell In: Stationary Phase

Swissprot (AC and ID): LDH3_BACAN (Q81XJ7)

Other databases:

- EMBL:   AE016879
- EMBL:   AE017334
- EMBL:   AE017225
- RefSeq:   NP_847419.1
- RefSeq:   YP_021893.1
- RefSeq:   YP_031111.1
- ProteinModelPortal:   Q81XJ7
- SMR:   Q81XJ7
- EnsemblBacteria:   EBBACT00000012539
- EnsemblBacteria:   EBBACT00000017872
- EnsemblBacteria:   EBBACT00000021809
- GeneID:   1084686
- GeneID:   2814538
- GeneID:   2848635
- GenomeReviews:   AE016879_GR
- GenomeReviews:   AE017225_GR
- GenomeReviews:   AE017334_GR
- KEGG:   ban:BA_5240
- KEGG:   bar:GBAA_5240
- KEGG:   bat:BAS4869
- TIGR:   BA_5240
- TIGR:   GBAA_5240
- GeneTree:   EBGT00050000001153
- HOGENOM:   HBG566126
- OMA:   REASDCA
- ProtClustDB:   PRK00066
- BioCyc:   BANT260799:BAS4869-MONOMER
- BioCyc:   BANT261594:GBAA5240-MONOMER
- BRENDA:   1.1.1.27
- GO:   GO:0005737
- GO:   GO:0005488
- GO:   GO:0006096
- HAMAP:   MF_00488
- InterPro:   IPR001557
- InterPro:   IPR011304
- InterPro:   IPR018177
- InterPro:   IPR022383
- InterPro:   IPR001236
- InterPro:   IPR015955
- InterPro:   IPR016040
- Gene3D:   G3DSA:3.90.110.10
- Gene3D:   G3DSA:3.40.50.720
- PIRSF:   PIRSF000102
- PRINTS:   PR00086
- TIGRFAMs:   TIGR01771

Pfam domain/function: PF02866 Ldh_1_C; PF00056 Ldh_1_N; SSF56327 Lactate_DH/Glyco_hydro_4_C

EC number: =1.1.1.27

Molecular weight: Translated: 34771; Mature: 34771

Theoretical pI: Translated: 4.99; Mature: 4.99

Prosite motif: PS00064 L_LDH; PS00013 PROKAR_LIPOPROTEIN

Important sites: ACT_SITE 178-178 BINDING 91-91 BINDING 123-123 BINDING 154-154 BINDING 233-233

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRHTRKIAIIGTGLVGSSCAYSIVNQGICEELLLIDINHERAVGEAMDLSHCINFTNTR
CCCCCEEEEEEECCCCCCHHHHHHHHHHHHHEEEEEECCCCHHHHHHHHHHHHHCCCCCC
TKVYAGSYEDCKDMDIVIITAGPAPKPGQSRLDTLGASAKIMESVVGGVMESGFDGIFLL
EEEEECCCCCCCCCCEEEEECCCCCCCCHHHHHHHCCHHHHHHHHHHHHHHCCCCEEEEE
ASNPVDIITYQVWKLSGLPRNRVIGTGTSLDSSRLRTILSEMLHVDPRSIHGYSLGEHGD
ECCCCEEEEEEEEEECCCCCCCEEECCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCC
SQMVAWSHVTVGGKPILQILEEQKERFGEIDLDEIVEKTAKAGWEIYKRKGTTYYGIGNS
CCEEEEEEEEECCHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCHHHHHHCCCEEEECCCH
LAYIASSIFNDDHRVIAVSAILDGEYGEYDICTGVPAIITRDGIREIVELNLTEDEESRF
HHHHHHHHHCCCCEEEEEEEEECCCCCCEEHHCCCCHHHHHHHHHHHHHCCCCCCHHHHH
AKSNDILRDYMKTIGY
HHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKRHTRKIAIIGTGLVGSSCAYSIVNQGICEELLLIDINHERAVGEAMDLSHCINFTNTR
CCCCCEEEEEEECCCCCCHHHHHHHHHHHHHEEEEEECCCCHHHHHHHHHHHHHCCCCCC
TKVYAGSYEDCKDMDIVIITAGPAPKPGQSRLDTLGASAKIMESVVGGVMESGFDGIFLL
EEEEECCCCCCCCCCEEEEECCCCCCCCHHHHHHHCCHHHHHHHHHHHHHHCCCCEEEEE
ASNPVDIITYQVWKLSGLPRNRVIGTGTSLDSSRLRTILSEMLHVDPRSIHGYSLGEHGD
ECCCCEEEEEEEEEECCCCCCCEEECCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCC
SQMVAWSHVTVGGKPILQILEEQKERFGEIDLDEIVEKTAKAGWEIYKRKGTTYYGIGNS
CCEEEEEEEEECCHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCHHHHHHCCCEEEECCCH
LAYIASSIFNDDHRVIAVSAILDGEYGEYDICTGVPAIITRDGIREIVELNLTEDEESRF
HHHHHHHHHCCCCEEEEEEEEECCCCCCEEHHCCCCHHHHHHHHHHHHHCCCCCCHHHHH
AKSNDILRDYMKTIGY
HHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12721629