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The map label for this gene is glyQS

Identifier: 49187774

GI number: 49187774

Start: 4674018

End: 4675394

Strand: Direct

Name: glyQS

Synonym: BAS4784

Alternate gene names: 49187774

Gene position: 4674018-4675394 (Clockwise)

Preceding gene: 49187773

Following gene: 49187776

Centisome position: 89.39

GC content: 40.45

Gene sequence:

>1377_bases
ATGTATTCAATGGAACAAGTAGTAAACTTAGCGAAACATCGCGGTTTTGTTTTCCCTGGTTCTGAAATTTACGGTGGTCT
TGCAAACACTTGGGATTACGGTCCACTTGGAATCGAATTAAAAAATAACGTTAAAAAAGCTTGGTGGAAAAAATTCATTC
AAGAATCTCCATACAACGTTGGTTTAGACGCAGCTATTTTAATGAACCCAAAAACTTGGATCGCTTCTGGTCACGTTGGT
AACTTCAACGATCCAATGATCGACTGTAAAAAATGTAAAGCGCGTCACCGTGCTGACAAATTAATTGAAGATGCATTAGA
TGCAAAAGGCATCGAAATGGTTGTTGATGGTCTTACTTTCGACCAAATGGCTGACTTAATGAAAGAACATGAAGTAAAAT
GTCCTGATTGCGGTAGTGAAGAATTCACTGAAATCCGTCAGTTCAACTTAATGTTCAAAACATTCCAAGGTGTTACAGAG
TCTAGCACAAACGAAATCTTCCTTCGTCCAGAAACAGCACAAGGTATTTTCGTAAACTTCAAAAACGTACAACGCTCTAT
GCGTAAAAAACTTCCATTTGGTATTGGCCAAATCGGTAAGAGCTTCCGTAACGAAATCACGCCTGGTAACTTCACATTCC
GTACACGTGAATTCGAACAAATGGAACTTGAATTCTTCTGTAAGCCTGGTGAAGATTTAGAGTGGTTCGCATTCTGGCGT
GAAACTTGTAAGAACTGGTTACTTTCACTTGGTATGACGGAAGAAAGCATGCGTCTTCGTGACCACGGTGAAGAGGAGTT
ATCTCACTACAGTAACGCAACAACTGATATTGAATTCAAATTCCCATTCGGTTGGGGCGAACTATGGGGCGTTGCATCTC
GTACAGACTTCGATTTAAAACGTCACATGGAACACTCTAACGAAGACTTTAACTATATCGATCCACAAACGAATGAGCGT
TACGTACCGTACTGCATCGAGCCATCTTTAGGTGCTGACCGCGTAACATTAGCATTCTTATGTGATGCATATGAAGAAGA
GCAATTAGAAAACGATTCTCGTACAGTTCTTCGTTTCCACCCTGCTTTAGCACCATACAAAGCAGCTATCTTACCATTAT
CTAAAAAGCTATCTGAAGGTGCTACTGAAGTATTCGCAGAATTAGCGAAAGACTTCATGGTAGACTTTGACGAAACTGGT
TCTATCGGTAAACGTTACCGTCGTCAAGACGAAATCGGTACACCATTCTGTATCACTTACGACTTCGACTCAGTTGAAGA
CAAAGCTGTTACAGTACGTGACCGTGACACAATGGAACAAGTTCGTATGCCAATTAGCGAACTAAAAGGTTTCTTAGAGA
AGAAAATCCAGTTCTAA

Upstream 100 bases:

>100_bases
GAGCGTTTTTTTATGTTTTTCAAAGCATATACTGCTCGTTTCACAAGTTACCTGAGCACGTCATCGCCAATACGTTAACT
TTCAAAAGGAGGATTTTATT

Downstream 100 bases:

>100_bases
TTACAAAAAAGAGGCGCTCTATTGAGCCGCCTCTTTTTTACTTTTCACGTTTTTCTTTAATTGCTACTGTACATCTTGAA
ATACATAATAAATCGTCATT

Product: glycyl-tRNA synthetase

Products: NA

Alternate protein names: Glycine--tRNA ligase; GlyRS

Number of amino acids: Translated: 458; Mature: 458

Protein sequence:

>458_residues
MYSMEQVVNLAKHRGFVFPGSEIYGGLANTWDYGPLGIELKNNVKKAWWKKFIQESPYNVGLDAAILMNPKTWIASGHVG
NFNDPMIDCKKCKARHRADKLIEDALDAKGIEMVVDGLTFDQMADLMKEHEVKCPDCGSEEFTEIRQFNLMFKTFQGVTE
SSTNEIFLRPETAQGIFVNFKNVQRSMRKKLPFGIGQIGKSFRNEITPGNFTFRTREFEQMELEFFCKPGEDLEWFAFWR
ETCKNWLLSLGMTEESMRLRDHGEEELSHYSNATTDIEFKFPFGWGELWGVASRTDFDLKRHMEHSNEDFNYIDPQTNER
YVPYCIEPSLGADRVTLAFLCDAYEEEQLENDSRTVLRFHPALAPYKAAILPLSKKLSEGATEVFAELAKDFMVDFDETG
SIGKRYRRQDEIGTPFCITYDFDSVEDKAVTVRDRDTMEQVRMPISELKGFLEKKIQF

Sequences:

>Translated_458_residues
MYSMEQVVNLAKHRGFVFPGSEIYGGLANTWDYGPLGIELKNNVKKAWWKKFIQESPYNVGLDAAILMNPKTWIASGHVG
NFNDPMIDCKKCKARHRADKLIEDALDAKGIEMVVDGLTFDQMADLMKEHEVKCPDCGSEEFTEIRQFNLMFKTFQGVTE
SSTNEIFLRPETAQGIFVNFKNVQRSMRKKLPFGIGQIGKSFRNEITPGNFTFRTREFEQMELEFFCKPGEDLEWFAFWR
ETCKNWLLSLGMTEESMRLRDHGEEELSHYSNATTDIEFKFPFGWGELWGVASRTDFDLKRHMEHSNEDFNYIDPQTNER
YVPYCIEPSLGADRVTLAFLCDAYEEEQLENDSRTVLRFHPALAPYKAAILPLSKKLSEGATEVFAELAKDFMVDFDETG
SIGKRYRRQDEIGTPFCITYDFDSVEDKAVTVRDRDTMEQVRMPISELKGFLEKKIQF
>Mature_458_residues
MYSMEQVVNLAKHRGFVFPGSEIYGGLANTWDYGPLGIELKNNVKKAWWKKFIQESPYNVGLDAAILMNPKTWIASGHVG
NFNDPMIDCKKCKARHRADKLIEDALDAKGIEMVVDGLTFDQMADLMKEHEVKCPDCGSEEFTEIRQFNLMFKTFQGVTE
SSTNEIFLRPETAQGIFVNFKNVQRSMRKKLPFGIGQIGKSFRNEITPGNFTFRTREFEQMELEFFCKPGEDLEWFAFWR
ETCKNWLLSLGMTEESMRLRDHGEEELSHYSNATTDIEFKFPFGWGELWGVASRTDFDLKRHMEHSNEDFNYIDPQTNER
YVPYCIEPSLGADRVTLAFLCDAYEEEQLENDSRTVLRFHPALAPYKAAILPLSKKLSEGATEVFAELAKDFMVDFDETG
SIGKRYRRQDEIGTPFCITYDFDSVEDKAVTVRDRDTMEQVRMPISELKGFLEKKIQF

Specific function: Catalyzes the attachment of glycine to tRNA(Gly)

COG id: COG0423

COG function: function code J; Glycyl-tRNA synthetase (class II)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI116805340, Length=379, Percent_Identity=31.1345646437995, Blast_Score=165, Evalue=8e-41,
Organism=Homo sapiens, GI70887790, Length=315, Percent_Identity=24.4444444444444, Blast_Score=79, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI32564541, Length=379, Percent_Identity=30.6068601583113, Blast_Score=160, Evalue=2e-39,
Organism=Caenorhabditis elegans, GI17554996, Length=379, Percent_Identity=30.6068601583113, Blast_Score=160, Evalue=2e-39,
Organism=Saccharomyces cerevisiae, GI6325338, Length=350, Percent_Identity=31.1428571428571, Blast_Score=155, Evalue=8e-39,
Organism=Saccharomyces cerevisiae, GI6319597, Length=398, Percent_Identity=28.643216080402, Blast_Score=151, Evalue=2e-37,
Organism=Drosophila melanogaster, GI21357965, Length=351, Percent_Identity=32.7635327635328, Blast_Score=176, Evalue=2e-44,
Organism=Drosophila melanogaster, GI24664462, Length=351, Percent_Identity=32.7635327635328, Blast_Score=176, Evalue=3e-44,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): SYG_BACAN (Q81XT3)

Other databases:

- EMBL:   AE016879
- EMBL:   AE017334
- EMBL:   AE017225
- RefSeq:   NP_847333.1
- RefSeq:   YP_021800.1
- RefSeq:   YP_031027.1
- ProteinModelPortal:   Q81XT3
- SMR:   Q81XT3
- IntAct:   Q81XT3
- EnsemblBacteria:   EBBACT00000010113
- EnsemblBacteria:   EBBACT00000017309
- EnsemblBacteria:   EBBACT00000021771
- GeneID:   1084520
- GeneID:   2815358
- GeneID:   2847859
- GenomeReviews:   AE016879_GR
- GenomeReviews:   AE017225_GR
- GenomeReviews:   AE017334_GR
- KEGG:   ban:BA_5147
- KEGG:   bar:GBAA_5147
- KEGG:   bat:BAS4784
- TIGR:   BA_5147
- TIGR:   GBAA_5147
- GeneTree:   EBGT00050000004274
- HOGENOM:   HBG319978
- OMA:   GSEWGEL
- ProtClustDB:   PRK04173
- BioCyc:   BANT260799:BAS4784-MONOMER
- BioCyc:   BANT261594:GBAA5147-MONOMER
- BRENDA:   6.1.1.14
- GO:   GO:0005737
- HAMAP:   MF_00253_B
- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR022961
- InterPro:   IPR002315
- Gene3D:   G3DSA:3.40.50.800
- PANTHER:   PTHR10745
- PRINTS:   PR01043
- TIGRFAMs:   TIGR00389

Pfam domain/function: PF03129 HGTP_anticodon; PF00587 tRNA-synt_2b; SSF52954 Anticodon_bd

EC number: =6.1.1.14

Molecular weight: Translated: 53019; Mature: 53019

Theoretical pI: Translated: 4.84; Mature: 4.84

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: BINDING 97-97 BINDING 171-171

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYSMEQVVNLAKHRGFVFPGSEIYGGLANTWDYGPLGIELKNNVKKAWWKKFIQESPYNV
CCCHHHHHHHHHHCCCCCCCHHHHCCCCCCCCCCCCCEEEHHHHHHHHHHHHHHCCCCCC
GLDAAILMNPKTWIASGHVGNFNDPMIDCKKCKARHRADKLIEDALDAKGIEMVVDGLTF
CCCEEEEECCCCEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCCCH
DQMADLMKEHEVKCPDCGSEEFTEIRQFNLMFKTFQGVTESSTNEIFLRPETAQGIFVNF
HHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCEEEEEH
KNVQRSMRKKLPFGIGQIGKSFRNEITPGNFTFRTREFEQMELEFFCKPGEDLEWFAFWR
HHHHHHHHHHCCCCHHHHHHHHHHCCCCCCEEEECCHHHHCCEEEEECCCCCHHHHHHHH
ETCKNWLLSLGMTEESMRLRDHGEEELSHYSNATTDIEFKFPFGWGELWGVASRTDFDLK
HHHHHHHHHCCCCHHHHHHHHCCHHHHHHHCCCCCEEEEECCCCHHHHHCCCCCCCHHHH
RHMEHSNEDFNYIDPQTNERYVPYCIEPSLGADRVTLAFLCDAYEEEQLENDSRTVLRFH
HHHHCCCCCCCCCCCCCCCCCCCEEECCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEEC
PALAPYKAAILPLSKKLSEGATEVFAELAKDFMVDFDETGSIGKRYRRQDEIGTPFCITY
CCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCEEEEE
DFDSVEDKAVTVRDRDTMEQVRMPISELKGFLEKKIQF
CCCCCCCCEEEECCHHHHHHHHCCHHHHHHHHHHHCCC
>Mature Secondary Structure
MYSMEQVVNLAKHRGFVFPGSEIYGGLANTWDYGPLGIELKNNVKKAWWKKFIQESPYNV
CCCHHHHHHHHHHCCCCCCCHHHHCCCCCCCCCCCCCEEEHHHHHHHHHHHHHHCCCCCC
GLDAAILMNPKTWIASGHVGNFNDPMIDCKKCKARHRADKLIEDALDAKGIEMVVDGLTF
CCCEEEEECCCCEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCCCH
DQMADLMKEHEVKCPDCGSEEFTEIRQFNLMFKTFQGVTESSTNEIFLRPETAQGIFVNF
HHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCEEEEEH
KNVQRSMRKKLPFGIGQIGKSFRNEITPGNFTFRTREFEQMELEFFCKPGEDLEWFAFWR
HHHHHHHHHHCCCCHHHHHHHHHHCCCCCCEEEECCHHHHCCEEEEECCCCCHHHHHHHH
ETCKNWLLSLGMTEESMRLRDHGEEELSHYSNATTDIEFKFPFGWGELWGVASRTDFDLK
HHHHHHHHHCCCCHHHHHHHHCCHHHHHHHCCCCCEEEEECCCCHHHHHCCCCCCCHHHH
RHMEHSNEDFNYIDPQTNERYVPYCIEPSLGADRVTLAFLCDAYEEEQLENDSRTVLRFH
HHHHCCCCCCCCCCCCCCCCCCCEEECCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEEC
PALAPYKAAILPLSKKLSEGATEVFAELAKDFMVDFDETGSIGKRYRRQDEIGTPFCITY
CCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCEEEEE
DFDSVEDKAVTVRDRDTMEQVRMPISELKGFLEKKIQF
CCCCCCCCEEEECCHHHHHHHHCCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12721629