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The map label for this gene is ldh

Identifier: 49187753

GI number: 49187753

Start: 4649560

End: 4650504

Strand: Reverse

Name: ldh

Synonym: BAS4762

Alternate gene names: 49187753

Gene position: 4650504-4649560 (Counterclockwise)

Preceding gene: 49187756

Following gene: 49187752

Centisome position: 88.94

GC content: 39.15

Gene sequence:

>945_bases
ATGAAAAAAGGTATCAATCGTGTAGTATTAGTAGGAACAGGAGCTGTAGGTTGTAGTTACGCTTACTCAATGATCAACCA
AGGTGTAGCTGAAGAATTCGTATTAGTAGATGTTAATGAAGCAAAAGCAGAAGGGGAAGCAATGGACTTAAGCCATGCGG
TACCATTCTCTCCATCACCAACAAAAGTTTGGAGCGGTAGCTATGCAGATTGTAAAGATGCAGATTTAGTAGTTATCACT
GCTGGATTACCACAAAAGCCAGGTGAGACTCGCTTAGACTTAGTTGAGAAAAATACAAAGATCTTTAAACAAATCGTTCG
CGGTATTATGGATAGCGGATTTGATGGAATCTTCTTAATCGCAACTAACCCAGTTGATATTTTAACTTACGTAACTTGGA
AAGAATCTGGTCTACCAAAAGAGCGCGTAATCGGTTCTGGTACTACTTTAGACTCTGCTCGTTTCCGTTATATGTTAGGT
GACTACTTAGATGTAGATCCACGTAACGTTCATGCTTACATCGTTGGTGAACACGGTGACACTGAACTTCCAGTATGGAG
CCACGCTACTATCGGTGTACAAAAACTAGAAACAATTTTAGCTAACAACGAACAGTACAAACAAGAAGACTTAGATAAAA
TTTTCGAAAACGTACGTGATGCAGCTTACCATATTATCGAGCGTAAAGGTGCAACTTACTACGGAATCGGTATGTCACTA
CTTCGTGTAACTAAAGCAATCTTAAACAACGAGAACAGCGTATTAACTGTATCTGCATACCTTGAAGGTCAATACGGTGA
GAAAGATGCTTACGTAGGTGTTCCAGCTGTCATTAACCGTGAAGGTGTACGTGAAATCGTAGAGCTTGAGTTAAATGAAG
AAGAAAAAGCGAAATTCGCTCATTCTGTAAAAGTATTAAAAGAAACAATGGCACCAGTACTATAA

Upstream 100 bases:

>100_bases
TATGAGTGTAAAGAAGTTAAACAACATTAAATTAGTATTTTAATTAATTACTTAAACAATCATAAAAAATATATGTGAAC
AATAGAGAGGGGAAATTACC

Downstream 100 bases:

>100_bases
TAATTGCTAAATAGTTCAGAGGCGGGAAACTGCTTCCTACCTCTGAATTTATATAATTTCATATAAATTCCTTTTCCGGT
TTCCCTTGTATATCCCCAAT

Product: L-lactate dehydrogenase

Products: NA

Alternate protein names: L-LDH 2

Number of amino acids: Translated: 314; Mature: 314

Protein sequence:

>314_residues
MKKGINRVVLVGTGAVGCSYAYSMINQGVAEEFVLVDVNEAKAEGEAMDLSHAVPFSPSPTKVWSGSYADCKDADLVVIT
AGLPQKPGETRLDLVEKNTKIFKQIVRGIMDSGFDGIFLIATNPVDILTYVTWKESGLPKERVIGSGTTLDSARFRYMLG
DYLDVDPRNVHAYIVGEHGDTELPVWSHATIGVQKLETILANNEQYKQEDLDKIFENVRDAAYHIIERKGATYYGIGMSL
LRVTKAILNNENSVLTVSAYLEGQYGEKDAYVGVPAVINREGVREIVELELNEEEKAKFAHSVKVLKETMAPVL

Sequences:

>Translated_314_residues
MKKGINRVVLVGTGAVGCSYAYSMINQGVAEEFVLVDVNEAKAEGEAMDLSHAVPFSPSPTKVWSGSYADCKDADLVVIT
AGLPQKPGETRLDLVEKNTKIFKQIVRGIMDSGFDGIFLIATNPVDILTYVTWKESGLPKERVIGSGTTLDSARFRYMLG
DYLDVDPRNVHAYIVGEHGDTELPVWSHATIGVQKLETILANNEQYKQEDLDKIFENVRDAAYHIIERKGATYYGIGMSL
LRVTKAILNNENSVLTVSAYLEGQYGEKDAYVGVPAVINREGVREIVELELNEEEKAKFAHSVKVLKETMAPVL
>Mature_314_residues
MKKGINRVVLVGTGAVGCSYAYSMINQGVAEEFVLVDVNEAKAEGEAMDLSHAVPFSPSPTKVWSGSYADCKDADLVVIT
AGLPQKPGETRLDLVEKNTKIFKQIVRGIMDSGFDGIFLIATNPVDILTYVTWKESGLPKERVIGSGTTLDSARFRYMLG
DYLDVDPRNVHAYIVGEHGDTELPVWSHATIGVQKLETILANNEQYKQEDLDKIFENVRDAAYHIIERKGATYYGIGMSL
LRVTKAILNNENSVLTVSAYLEGQYGEKDAYVGVPAVINREGVREIVELELNEEEKAKFAHSVKVLKETMAPVL

Specific function: Catalyzes The Reversible Oxidation Of Malate To Oxaloacetate. [C]

COG id: COG0039

COG function: function code C; Malate/lactate dehydrogenases

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the LDH/MDH superfamily. LDH family

Homologues:

Organism=Homo sapiens, GI47059044, Length=305, Percent_Identity=42.2950819672131, Blast_Score=244, Evalue=6e-65,
Organism=Homo sapiens, GI221136809, Length=305, Percent_Identity=42.2950819672131, Blast_Score=244, Evalue=6e-65,
Organism=Homo sapiens, GI5031857, Length=303, Percent_Identity=40.5940594059406, Blast_Score=239, Evalue=2e-63,
Organism=Homo sapiens, GI260099723, Length=303, Percent_Identity=40.5940594059406, Blast_Score=239, Evalue=2e-63,
Organism=Homo sapiens, GI15082234, Length=305, Percent_Identity=39.0163934426229, Blast_Score=238, Evalue=4e-63,
Organism=Homo sapiens, GI9257228, Length=305, Percent_Identity=42.2950819672131, Blast_Score=237, Evalue=1e-62,
Organism=Homo sapiens, GI4504973, Length=305, Percent_Identity=42.2950819672131, Blast_Score=237, Evalue=1e-62,
Organism=Homo sapiens, GI291575128, Length=305, Percent_Identity=38.6885245901639, Blast_Score=234, Evalue=7e-62,
Organism=Homo sapiens, GI4557032, Length=305, Percent_Identity=38.6885245901639, Blast_Score=234, Evalue=7e-62,
Organism=Homo sapiens, GI260099725, Length=226, Percent_Identity=43.8053097345133, Blast_Score=189, Evalue=2e-48,
Organism=Homo sapiens, GI260099727, Length=225, Percent_Identity=44, Blast_Score=189, Evalue=3e-48,
Organism=Homo sapiens, GI207028494, Length=209, Percent_Identity=38.755980861244, Blast_Score=155, Evalue=4e-38,
Organism=Homo sapiens, GI103472011, Length=313, Percent_Identity=25.8785942492013, Blast_Score=127, Evalue=9e-30,
Organism=Homo sapiens, GI103472015, Length=185, Percent_Identity=29.7297297297297, Blast_Score=100, Evalue=3e-21,
Organism=Homo sapiens, GI21735621, Length=292, Percent_Identity=24.6575342465753, Blast_Score=77, Evalue=2e-14,
Organism=Escherichia coli, GI1789632, Length=296, Percent_Identity=26.3513513513513, Blast_Score=70, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17535107, Length=297, Percent_Identity=40.4040404040404, Blast_Score=229, Evalue=1e-60,
Organism=Caenorhabditis elegans, GI17554310, Length=305, Percent_Identity=28.8524590163934, Blast_Score=75, Evalue=5e-14,
Organism=Saccharomyces cerevisiae, GI6322765, Length=155, Percent_Identity=31.6129032258064, Blast_Score=66, Evalue=9e-12,
Organism=Drosophila melanogaster, GI17136226, Length=310, Percent_Identity=40.6451612903226, Blast_Score=247, Evalue=7e-66,
Organism=Drosophila melanogaster, GI45550422, Length=307, Percent_Identity=29.3159609120521, Blast_Score=166, Evalue=1e-41,
Organism=Drosophila melanogaster, GI24647881, Length=317, Percent_Identity=27.1293375394322, Blast_Score=92, Evalue=3e-19,
Organism=Drosophila melanogaster, GI24663599, Length=326, Percent_Identity=23.6196319018405, Blast_Score=87, Evalue=2e-17,

Paralogues:

None

Copy number: 2640 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 260 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2380 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). 260 Molecules/Cell In: Stationary Phase

Swissprot (AC and ID): LDH2_BACAN (Q81K80)

Other databases:

- EMBL:   AE016879
- EMBL:   AE017334
- EMBL:   AE017225
- RefSeq:   NP_847311.1
- RefSeq:   YP_021778.1
- RefSeq:   YP_031006.1
- ProteinModelPortal:   Q81K80
- SMR:   Q81K80
- EnsemblBacteria:   EBBACT00000008590
- EnsemblBacteria:   EBBACT00000016883
- EnsemblBacteria:   EBBACT00000021286
- GeneID:   1084439
- GeneID:   2819768
- GeneID:   2850586
- GenomeReviews:   AE016879_GR
- GenomeReviews:   AE017225_GR
- GenomeReviews:   AE017334_GR
- KEGG:   ban:BA_5125
- KEGG:   bar:GBAA_5125
- KEGG:   bat:BAS4762
- TIGR:   BA_5125
- TIGR:   GBAA_5125
- GeneTree:   EBGT00050000001153
- HOGENOM:   HBG566126
- OMA:   MDLNHGV
- ProtClustDB:   PRK00066
- BioCyc:   BANT260799:BAS4762-MONOMER
- BioCyc:   BANT261594:GBAA5125-MONOMER
- BRENDA:   1.1.1.27
- GO:   GO:0005737
- GO:   GO:0005488
- GO:   GO:0006096
- HAMAP:   MF_00488
- InterPro:   IPR001557
- InterPro:   IPR011304
- InterPro:   IPR018177
- InterPro:   IPR022383
- InterPro:   IPR001236
- InterPro:   IPR015955
- InterPro:   IPR016040
- Gene3D:   G3DSA:3.90.110.10
- Gene3D:   G3DSA:3.40.50.720
- PIRSF:   PIRSF000102
- PRINTS:   PR00086
- TIGRFAMs:   TIGR01771

Pfam domain/function: PF02866 Ldh_1_C; PF00056 Ldh_1_N; SSF56327 Lactate_DH/Glyco_hydro_4_C

EC number: =1.1.1.27

Molecular weight: Translated: 34621; Mature: 34621

Theoretical pI: Translated: 4.83; Mature: 4.83

Prosite motif: PS00064 L_LDH; PS00013 PROKAR_LIPOPROTEIN

Important sites: ACT_SITE 178-178 BINDING 91-91 BINDING 123-123 BINDING 154-154 BINDING 232-232

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKGINRVVLVGTGAVGCSYAYSMINQGVAEEFVLVDVNEAKAEGEAMDLSHAVPFSPSP
CCCCCCEEEEEECCCHHHHHHHHHHHCCCCCEEEEEECCHHCCCCCCCCCCCCCCCCCCC
TKVWSGSYADCKDADLVVITAGLPQKPGETRLDLVEKNTKIFKQIVRGIMDSGFDGIFLI
CCCCCCCCCCCCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEE
ATNPVDILTYVTWKESGLPKERVIGSGTTLDSARFRYMLGDYLDVDPRNVHAYIVGEHGD
ECCCEEEEEEEEECCCCCCHHHEECCCCCCCHHHHHHHHCCCCCCCCCCEEEEEEECCCC
TELPVWSHATIGVQKLETILANNEQYKQEDLDKIFENVRDAAYHIIERKGATYYGIGMSL
CCCCEECCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEHHHHHH
LRVTKAILNNENSVLTVSAYLEGQYGEKDAYVGVPAVINREGVREIVELELNEEEKAKFA
HHHHHHHHCCCCCEEEEEEEECCCCCCCCCEECCCHHHCHHHHHHHHHHCCCCHHHHHHH
HSVKVLKETMAPVL
HHHHHHHHHHCCCC
>Mature Secondary Structure
MKKGINRVVLVGTGAVGCSYAYSMINQGVAEEFVLVDVNEAKAEGEAMDLSHAVPFSPSP
CCCCCCEEEEEECCCHHHHHHHHHHHCCCCCEEEEEECCHHCCCCCCCCCCCCCCCCCCC
TKVWSGSYADCKDADLVVITAGLPQKPGETRLDLVEKNTKIFKQIVRGIMDSGFDGIFLI
CCCCCCCCCCCCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEE
ATNPVDILTYVTWKESGLPKERVIGSGTTLDSARFRYMLGDYLDVDPRNVHAYIVGEHGD
ECCCEEEEEEEEECCCCCCHHHEECCCCCCCHHHHHHHHCCCCCCCCCCEEEEEEECCCC
TELPVWSHATIGVQKLETILANNEQYKQEDLDKIFENVRDAAYHIIERKGATYYGIGMSL
CCCCEECCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEHHHHHH
LRVTKAILNNENSVLTVSAYLEGQYGEKDAYVGVPAVINREGVREIVELELNEEEKAKFA
HHHHHHHHCCCCCEEEEEEEECCCCCCCCCEECCCHHHCHHHHHHHHHHCCCCHHHHHHH
HSVKVLKETMAPVL
HHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12721629