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The map label for this gene is kinE [H]

Identifier: 49187660

GI number: 49187660

Start: 4562515

End: 4563771

Strand: Reverse

Name: kinE [H]

Synonym: BAS4669

Alternate gene names: 49187660

Gene position: 4563771-4562515 (Counterclockwise)

Preceding gene: 49187662

Following gene: 49187659

Centisome position: 87.28

GC content: 32.06

Gene sequence:

>1257_bases
GTGAAAGACAGTTTTCTATCATATAAAGAAGAAGAAAGGAATGCAAAAATCCTTTTGTGGGTGCTATATGTCATTGTTGT
TTCATACCAAATTTTCTATGCAATTGTATTAGAGAATAAATCACTAACAGATAAGGGATATAGCGTACTATGGCAAGTTA
TTTGTGGAACTGCAATACTTGGTGTGAATATATATTTAATAAAAAAAGAGAAAGCGAATTTCGTTAAATATGCATGTGTA
TTTGCATACTTAGGAATAGAGATTGCTAATATATTTTCGTATATGCTTTATAATGAGGCGGCATTTGATGGGGTAAATAT
AATAGAAATTATTCTCATCTTCTTTGTGCCTATATTTTTAAATAAAAAATATTTTGTGTTTATACTTTCAACTATTGTAG
TGAAATATGTTATTTACTTATTTGTATTAAAGGAAGTAAAAGGATTTATGTACCTCATTATGTATACACTCGTATTAATA
GCAGCATACATCATTTTAAATCGATTTATACAATACCTTTCAGCAGTAAAGGAAAGCATTAAGGTTGCTAGTGAATCACA
AAAATTAGCAGTAATCGGAAAAATGGCAGCGACAGTGGGACACGAAATTAAAAATCCACTTGCTTCATTAAAAGGGTTTA
CGCAATTACAACGAGAGAAGCATGGGGAGGACCCTATTTACAAACAGATGATTTTTGAAATAGAAAATATGAATAATATG
ATAAGTGAATTGATGGAAGTTGCTACATGTAAACCCTCTATTTATAAGAAACATAATATAGGTGAAATTGTATTACAAGC
AGTAGCAATTCTTCGCGAAAAAATGAATGAATGTAGTGTCCAGCTTATTTCTAATGTTGAAGAAAGCATAATAGAAGTTG
AATGTGATGAGCGGAAAATGCGAGGAGTTTTTCTATATGTCATAAAAAATGCTCTGGAGGCAATGGAGCAAGGTGGCACA
TTAGAGTTACGGTTAGAAAATAAAGGACAAGATTATGTAATGCTAAGTGTAATCGATAATGGTTACGGTATTAAGAAAGA
AAATTTGGCTCGTGTTACAGAAGCCTTTTATACAACGAAACAAGATAAGATTGGTTTGGGACTTACGGTAGCGAATCGAC
TTGTTACGGAGCAGCATCTTGGTAAATTACATATTTCAAGTGAAAGAGATATTGGAACTAGAATAGATATCATTCTCCCG
AAACAACGTGGAAATAGTGAAATCCAAGTGGGAGAAAAGCTAGGAGTTACCATATGA

Upstream 100 bases:

>100_bases
TGATTTACCTCAAATGTTCGATTTCTCTTTGTTTGTCTAAGAAAATCATTTATTATAATAAATGGTATTACTTCGAGATT
TTTTTTGAAGGGGTGTTGAA

Downstream 100 bases:

>100_bases
ATAAAGACGGTATATTTAAAAATGAAGAAATGAAGGCGCTAAAAATATTTTTAAGTTTATTTTTCATTATATTTTTTGTA
TACGATCTTGCCTACGAATT

Product: sensor histidine kinase

Products: NA

Alternate protein names: Sensor histidine kinase E [H]

Number of amino acids: Translated: 418; Mature: 418

Protein sequence:

>418_residues
MKDSFLSYKEEERNAKILLWVLYVIVVSYQIFYAIVLENKSLTDKGYSVLWQVICGTAILGVNIYLIKKEKANFVKYACV
FAYLGIEIANIFSYMLYNEAAFDGVNIIEIILIFFVPIFLNKKYFVFILSTIVVKYVIYLFVLKEVKGFMYLIMYTLVLI
AAYIILNRFIQYLSAVKESIKVASESQKLAVIGKMAATVGHEIKNPLASLKGFTQLQREKHGEDPIYKQMIFEIENMNNM
ISELMEVATCKPSIYKKHNIGEIVLQAVAILREKMNECSVQLISNVEESIIEVECDERKMRGVFLYVIKNALEAMEQGGT
LELRLENKGQDYVMLSVIDNGYGIKKENLARVTEAFYTTKQDKIGLGLTVANRLVTEQHLGKLHISSERDIGTRIDIILP
KQRGNSEIQVGEKLGVTI

Sequences:

>Translated_418_residues
MKDSFLSYKEEERNAKILLWVLYVIVVSYQIFYAIVLENKSLTDKGYSVLWQVICGTAILGVNIYLIKKEKANFVKYACV
FAYLGIEIANIFSYMLYNEAAFDGVNIIEIILIFFVPIFLNKKYFVFILSTIVVKYVIYLFVLKEVKGFMYLIMYTLVLI
AAYIILNRFIQYLSAVKESIKVASESQKLAVIGKMAATVGHEIKNPLASLKGFTQLQREKHGEDPIYKQMIFEIENMNNM
ISELMEVATCKPSIYKKHNIGEIVLQAVAILREKMNECSVQLISNVEESIIEVECDERKMRGVFLYVIKNALEAMEQGGT
LELRLENKGQDYVMLSVIDNGYGIKKENLARVTEAFYTTKQDKIGLGLTVANRLVTEQHLGKLHISSERDIGTRIDIILP
KQRGNSEIQVGEKLGVTI
>Mature_418_residues
MKDSFLSYKEEERNAKILLWVLYVIVVSYQIFYAIVLENKSLTDKGYSVLWQVICGTAILGVNIYLIKKEKANFVKYACV
FAYLGIEIANIFSYMLYNEAAFDGVNIIEIILIFFVPIFLNKKYFVFILSTIVVKYVIYLFVLKEVKGFMYLIMYTLVLI
AAYIILNRFIQYLSAVKESIKVASESQKLAVIGKMAATVGHEIKNPLASLKGFTQLQREKHGEDPIYKQMIFEIENMNNM
ISELMEVATCKPSIYKKHNIGEIVLQAVAILREKMNECSVQLISNVEESIIEVECDERKMRGVFLYVIKNALEAMEQGGT
LELRLENKGQDYVMLSVIDNGYGIKKENLARVTEAFYTTKQDKIGLGLTVANRLVTEQHLGKLHISSERDIGTRIDIILP
KQRGNSEIQVGEKLGVTI

Specific function: Phosphorylates the sporulation-regulatory protein spo0A under biofilm growth conditions. Also able to weakly phosphorylate spo0F [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Integral Membrane Protein. Inner Membrane [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 4 PAS (PER-ARNT-SIM) domains [H]

Homologues:

Organism=Escherichia coli, GI1788549, Length=241, Percent_Identity=25.7261410788382, Blast_Score=112, Evalue=5e-26,
Organism=Escherichia coli, GI1790436, Length=248, Percent_Identity=26.2096774193548, Blast_Score=103, Evalue=1e-23,
Organism=Escherichia coli, GI1790300, Length=203, Percent_Identity=28.0788177339901, Blast_Score=68, Evalue=9e-13,
Organism=Escherichia coli, GI1786600, Length=225, Percent_Identity=23.1111111111111, Blast_Score=62, Evalue=8e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR000014
- InterPro:   IPR013767
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA; PF00989 PAS [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 47688; Mature: 47688

Theoretical pI: Translated: 8.14; Mature: 8.14

Prosite motif: PS50109 HIS_KIN ; PS00838 INTERLEUKIN_4_13

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKDSFLSYKEEERNAKILLWVLYVIVVSYQIFYAIVLENKSLTDKGYSVLWQVICGTAIL
CCCCCCCCHHHCCCCHHHHHHHHHHHHHHHHHHHHEECCCCCCHHHHHHHHHHHHHHHHH
GVNIYLIKKEKANFVKYACVFAYLGIEIANIFSYMLYNEAAFDGVNIIEIILIFFVPIFL
CCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
NKKYFVFILSTIVVKYVIYLFVLKEVKGFMYLIMYTLVLIAAYIILNRFIQYLSAVKESI
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KVASESQKLAVIGKMAATVGHEIKNPLASLKGFTQLQREKHGEDPIYKQMIFEIENMNNM
HHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHH
ISELMEVATCKPSIYKKHNIGEIVLQAVAILREKMNECSVQLISNVEESIIEVECDERKM
HHHHHHHHHCCCHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCHHH
RGVFLYVIKNALEAMEQGGTLELRLENKGQDYVMLSVIDNGYGIKKENLARVTEAFYTTK
HHHHHHHHHHHHHHHHCCCEEEEEECCCCCCEEEEEEEECCCCCCHHHHHHHHHHHHHCC
QDKIGLGLTVANRLVTEQHLGKLHISSERDIGTRIDIILPKQRGNSEIQVGEKLGVTI
CCCCCCHHHHHHHHHHHHHHCCEEECCCCCCCCEEEEEEECCCCCCCEEECCCCCCCC
>Mature Secondary Structure
MKDSFLSYKEEERNAKILLWVLYVIVVSYQIFYAIVLENKSLTDKGYSVLWQVICGTAIL
CCCCCCCCHHHCCCCHHHHHHHHHHHHHHHHHHHHEECCCCCCHHHHHHHHHHHHHHHHH
GVNIYLIKKEKANFVKYACVFAYLGIEIANIFSYMLYNEAAFDGVNIIEIILIFFVPIFL
CCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
NKKYFVFILSTIVVKYVIYLFVLKEVKGFMYLIMYTLVLIAAYIILNRFIQYLSAVKESI
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KVASESQKLAVIGKMAATVGHEIKNPLASLKGFTQLQREKHGEDPIYKQMIFEIENMNNM
HHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHH
ISELMEVATCKPSIYKKHNIGEIVLQAVAILREKMNECSVQLISNVEESIIEVECDERKM
HHHHHHHHHCCCHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCHHH
RGVFLYVIKNALEAMEQGGTLELRLENKGQDYVMLSVIDNGYGIKKENLARVTEAFYTTK
HHHHHHHHHHHHHHHHCCCEEEEEECCCCCCEEEEEEEECCCCCCHHHHHHHHHHHHHCC
QDKIGLGLTVANRLVTEQHLGKLHISSERDIGTRIDIILPKQRGNSEIQVGEKLGVTI
CCCCCCHHHHHHHHHHHHHHCCEEECCCCCCCCEEEEEEECCCCCCCEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]