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The map label for this gene is pckA

Identifier: 49187652

GI number: 49187652

Start: 4555133

End: 4556719

Strand: Direct

Name: pckA

Synonym: BAS4661

Alternate gene names: 49187652

Gene position: 4555133-4556719 (Clockwise)

Preceding gene: 49187648

Following gene: 49187654

Centisome position: 87.12

GC content: 38.69

Gene sequence:

>1587_bases
ATGAGTACTGTGAATGTCCAAATTGGTTTACACGAATTATTGAACGGAAGCAATGCACAGATTCAACTAAGTGTTCCGCA
ATTAGTGGAAAAAGTATTAATGCGAAATGAAGGGAAATTAACTTCGACTGGTGCCGTTTCTGCTTCAACTGGAAAATATA
CAGGACGTTCTCCTAAAGATAAATTTATTGTGAAAGAAGCATCGGTTGCTGACAAAATTGCTTGGGGAGCTGTGAATCAA
CCGATTTCTGAAGAACATTTTAATAAATTATATACTAAAGTTTTAGAATACTTAAAAGAAAAAGAAGAGTTATTCGTCTT
CAAAGGATTTGCAGGCGCGGACCGCAATTACCGTCTACCAATCCAAGTTATTAATGAATATGCATGGCACAATTTATTTG
TACATCAATTATTCATTCGTCCAACTGAAGAAGAATTAACGACTCATGAATCAGAATTCACAATTGTTTCTGCACCAAAT
TTCAAAGCTGATCCAGCTGTTGACGGTACAAACTCTGAAGCATTCATTATGGTTTCATTCGAAAAACGTATCGTACTAAT
TGGTGGTACAGAATACGCTGGAGAAATGAAAAAATCAATCTTCTCTATTATGAACTTCTTACTACCTGAACAAGATATTC
TTTCTATGCATTGCTCTGCAAACGTAGGTGAAGAAGGCGACGTAGCACTATTCTTCGGTTTATCTGGAACAGGTAAAACA
ACATTATCTGCTGATCCAAACCGTAAATTAATCGGTGATGATGAGCATGGTTGGTCTGATAACGGTGTATTCAATATTGA
AGGCGGTTGCTACGCAAAATGTGTAAACCTTTCTCACGAGAAAGAACCACAAATTTTCGATGCAATCACATTTGGATCTG
TTTTAGAAAACGTTATCATTAATGACCAAACGCGTATCGCAGACTATAACGATACTACTTTAACAGAAAATACTCGTGCT
GCATACCCTATGCATGCGATCGACAATATCATACTGCCAAGTGTTGCTGGACACCCAAATACAATTATTTTCTTAACTGC
TGATGCATCTGGCGTATTGCCTCCAATCAGCAAGTTATCAAAAGAACAAGCTATGTACCATTTCTTAAGTGGTTACACTA
GTAAACTAGCAGGAACAGAGCGCGGCGTTACATCACCGCAAGCAACATTCTCAACTTGCTTCGGTTCACCATTCTTACCA
CTTGATGCATCTCGCTATGCTGAAATGCTTGGTGAGAAAATCGAGAAACACGATGCAAAAGTATTCTTAGTAAACACTGG
CTGGACTGGTGGCGAATACGGCGTTGGTAAACGTATGAACTTAGGTTACACTCGTGCAATGATTCAAGCAGCATTAAACG
GTGAACTTGCGAAAACTGAAACAGCTAAACATGACATCTTCGGTCTTGAAGTTCCACTTCACGTACCAGGTGTACCTGAC
GAAGTGTTAATGCCTGAACAAACTTGGGCTGATAAAGCTGCTTACAAAGCAAAAGCAATCGAACTTGCAAATGAATTCAA
AGCGAACTTCAAAAAGTTCGACAGCGTATCTGAAGACATTATTAACTTAGGCGGTCCAATCGCTTAA

Upstream 100 bases:

>100_bases
AAAAAGAAATAAATAGTATACATTATTTATATAATTGTGTTATACTATTGTTGGGGATTACGAGAAATATTTCATTAAAT
GAAAAGGATGGTATATAAAT

Downstream 100 bases:

>100_bases
GTGCAAACTTTCTGTTGGAATAACCCTTTACTCATATGAATAAGTGTCGTGTAACCGTAAAGTTTCGCGTACTCACCGAC
AAAACCCAATGCGGCTTAAC

Product: phosphoenolpyruvate carboxykinase

Products: NA

Alternate protein names: PEP carboxykinase; PEPCK; Phosphoenolpyruvate carboxylase

Number of amino acids: Translated: 528; Mature: 527

Protein sequence:

>528_residues
MSTVNVQIGLHELLNGSNAQIQLSVPQLVEKVLMRNEGKLTSTGAVSASTGKYTGRSPKDKFIVKEASVADKIAWGAVNQ
PISEEHFNKLYTKVLEYLKEKEELFVFKGFAGADRNYRLPIQVINEYAWHNLFVHQLFIRPTEEELTTHESEFTIVSAPN
FKADPAVDGTNSEAFIMVSFEKRIVLIGGTEYAGEMKKSIFSIMNFLLPEQDILSMHCSANVGEEGDVALFFGLSGTGKT
TLSADPNRKLIGDDEHGWSDNGVFNIEGGCYAKCVNLSHEKEPQIFDAITFGSVLENVIINDQTRIADYNDTTLTENTRA
AYPMHAIDNIILPSVAGHPNTIIFLTADASGVLPPISKLSKEQAMYHFLSGYTSKLAGTERGVTSPQATFSTCFGSPFLP
LDASRYAEMLGEKIEKHDAKVFLVNTGWTGGEYGVGKRMNLGYTRAMIQAALNGELAKTETAKHDIFGLEVPLHVPGVPD
EVLMPEQTWADKAAYKAKAIELANEFKANFKKFDSVSEDIINLGGPIA

Sequences:

>Translated_528_residues
MSTVNVQIGLHELLNGSNAQIQLSVPQLVEKVLMRNEGKLTSTGAVSASTGKYTGRSPKDKFIVKEASVADKIAWGAVNQ
PISEEHFNKLYTKVLEYLKEKEELFVFKGFAGADRNYRLPIQVINEYAWHNLFVHQLFIRPTEEELTTHESEFTIVSAPN
FKADPAVDGTNSEAFIMVSFEKRIVLIGGTEYAGEMKKSIFSIMNFLLPEQDILSMHCSANVGEEGDVALFFGLSGTGKT
TLSADPNRKLIGDDEHGWSDNGVFNIEGGCYAKCVNLSHEKEPQIFDAITFGSVLENVIINDQTRIADYNDTTLTENTRA
AYPMHAIDNIILPSVAGHPNTIIFLTADASGVLPPISKLSKEQAMYHFLSGYTSKLAGTERGVTSPQATFSTCFGSPFLP
LDASRYAEMLGEKIEKHDAKVFLVNTGWTGGEYGVGKRMNLGYTRAMIQAALNGELAKTETAKHDIFGLEVPLHVPGVPD
EVLMPEQTWADKAAYKAKAIELANEFKANFKKFDSVSEDIINLGGPIA
>Mature_527_residues
STVNVQIGLHELLNGSNAQIQLSVPQLVEKVLMRNEGKLTSTGAVSASTGKYTGRSPKDKFIVKEASVADKIAWGAVNQP
ISEEHFNKLYTKVLEYLKEKEELFVFKGFAGADRNYRLPIQVINEYAWHNLFVHQLFIRPTEEELTTHESEFTIVSAPNF
KADPAVDGTNSEAFIMVSFEKRIVLIGGTEYAGEMKKSIFSIMNFLLPEQDILSMHCSANVGEEGDVALFFGLSGTGKTT
LSADPNRKLIGDDEHGWSDNGVFNIEGGCYAKCVNLSHEKEPQIFDAITFGSVLENVIINDQTRIADYNDTTLTENTRAA
YPMHAIDNIILPSVAGHPNTIIFLTADASGVLPPISKLSKEQAMYHFLSGYTSKLAGTERGVTSPQATFSTCFGSPFLPL
DASRYAEMLGEKIEKHDAKVFLVNTGWTGGEYGVGKRMNLGYTRAMIQAALNGELAKTETAKHDIFGLEVPLHVPGVPDE
VLMPEQTWADKAAYKAKAIELANEFKANFKKFDSVSEDIINLGGPIA

Specific function: Rate-limiting gluconeogenic enzyme. [C]

COG id: COG1866

COG function: function code C; Phosphoenolpyruvate carboxykinase (ATP)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphoenolpyruvate carboxykinase [ATP] family

Homologues:

Organism=Escherichia coli, GI1789807, Length=489, Percent_Identity=49.8977505112474, Blast_Score=476, Evalue=1e-135,
Organism=Saccharomyces cerevisiae, GI6322950, Length=504, Percent_Identity=51.1904761904762, Blast_Score=514, Evalue=1e-146,

Paralogues:

None

Copy number: 20 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): PCKA_BACAA (C3PCC6)

Other databases:

- EMBL:   CP001598
- RefSeq:   YP_002869045.1
- ProteinModelPortal:   C3PCC6
- SMR:   C3PCC6
- EnsemblBacteria:   EBBACT00000125976
- GeneID:   7849490
- GenomeReviews:   CP001598_GR
- KEGG:   bai:BAA_5034
- GeneTree:   EBGT00050000001183
- ProtClustDB:   PRK09344
- GO:   GO:0005737
- GO:   GO:0006094
- HAMAP:   MF_00453
- InterPro:   IPR001272
- InterPro:   IPR013035
- InterPro:   IPR008210
- InterPro:   IPR015994
- Gene3D:   G3DSA:3.90.228.20
- Gene3D:   G3DSA:3.40.449.10
- PIRSF:   PIRSF006294
- TIGRFAMs:   TIGR00224

Pfam domain/function: PF01293 PEPCK_ATP; SSF68923 PEP_carboxykinase_N

EC number: =4.1.1.49

Molecular weight: Translated: 57966; Mature: 57835

Theoretical pI: Translated: 5.09; Mature: 5.09

Prosite motif: PS00532 PEPCK_ATP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTVNVQIGLHELLNGSNAQIQLSVPQLVEKVLMRNEGKLTSTGAVSASTGKYTGRSPKD
CCEEEEEEEHHHHHCCCCCEEEEEHHHHHHHHHHCCCCCEEECCCEECCCCCCCCCCCCC
KFIVKEASVADKIAWGAVNQPISEEHFNKLYTKVLEYLKEKEELFVFKGFAGADRNYRLP
CEEEEHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCH
IQVINEYAWHNLFVHQLFIRPTEEELTTHESEFTIVSAPNFKADPAVDGTNSEAFIMVSF
HHHHHHHHHHHHEEHHHEECCCHHHHHCCCCCEEEEECCCCCCCCCCCCCCCCEEEEEEE
EKRIVLIGGTEYAGEMKKSIFSIMNFLLPEQDILSMHCSANVGEEGDVALFFGLSGTGKT
CCEEEEEECCHHHHHHHHHHHHHHHHHCCCHHHHHEECCCCCCCCCCEEEEEEECCCCCE
TLSADPNRKLIGDDEHGWSDNGVFNIEGGCYAKCVNLSHEKEPQIFDAITFGSVLENVII
EECCCCCCCCCCCCCCCCCCCCEEEECCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHC
NDQTRIADYNDTTLTENTRAAYPMHAIDNIILPSVAGHPNTIIFLTADASGVLPPISKLS
CCCCEEECCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCEEEEEEECCCCCCCCHHHHH
KEQAMYHFLSGYTSKLAGTERGVTSPQATFSTCFGSPFLPLDASRYAEMLGEKIEKHDAK
HHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCE
VFLVNTGWTGGEYGVGKRMNLGYTRAMIQAALNGELAKTETAKHDIFGLEVPLHVPGVPD
EEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHCCCHHHCCCCCCCEEEEEEEEECCCCCH
EVLMPEQTWADKAAYKAKAIELANEFKANFKKFDSVSEDIINLGGPIA
HHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
STVNVQIGLHELLNGSNAQIQLSVPQLVEKVLMRNEGKLTSTGAVSASTGKYTGRSPKD
CEEEEEEEHHHHHCCCCCEEEEEHHHHHHHHHHCCCCCEEECCCEECCCCCCCCCCCCC
KFIVKEASVADKIAWGAVNQPISEEHFNKLYTKVLEYLKEKEELFVFKGFAGADRNYRLP
CEEEEHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCH
IQVINEYAWHNLFVHQLFIRPTEEELTTHESEFTIVSAPNFKADPAVDGTNSEAFIMVSF
HHHHHHHHHHHHEEHHHEECCCHHHHHCCCCCEEEEECCCCCCCCCCCCCCCCEEEEEEE
EKRIVLIGGTEYAGEMKKSIFSIMNFLLPEQDILSMHCSANVGEEGDVALFFGLSGTGKT
CCEEEEEECCHHHHHHHHHHHHHHHHHCCCHHHHHEECCCCCCCCCCEEEEEEECCCCCE
TLSADPNRKLIGDDEHGWSDNGVFNIEGGCYAKCVNLSHEKEPQIFDAITFGSVLENVII
EECCCCCCCCCCCCCCCCCCCCEEEECCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHC
NDQTRIADYNDTTLTENTRAAYPMHAIDNIILPSVAGHPNTIIFLTADASGVLPPISKLS
CCCCEEECCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCEEEEEEECCCCCCCCHHHHH
KEQAMYHFLSGYTSKLAGTERGVTSPQATFSTCFGSPFLPLDASRYAEMLGEKIEKHDAK
HHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCE
VFLVNTGWTGGEYGVGKRMNLGYTRAMIQAALNGELAKTETAKHDIFGLEVPLHVPGVPD
EEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHCCCHHHCCCCCCCEEEEEEEEECCCCCH
EVLMPEQTWADKAAYKAKAIELANEFKANFKKFDSVSEDIINLGGPIA
HHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA