Click here to switch to the map view.

The map label for this gene is metK

Identifier: 49187651

GI number: 49187651

Start: 4553414

End: 4554613

Strand: Reverse

Name: metK

Synonym: BAS4660

Alternate gene names: 49187651

Gene position: 4554613-4553414 (Counterclockwise)

Preceding gene: 49187653

Following gene: 49187650

Centisome position: 87.11

GC content: 41.58

Gene sequence:

>1200_bases
ATGACAAAAAAACGTCATCTGTTCACATCTGAGTCTGTAACTGAAGGACATCCAGATAAAATTTGTGACCAAATTTCTGA
TTCAATTTTAGATGCGATCTTATCAAAAGACGCAAATGCACGTGTAGCTTGTGAAACAACTGTAACAACTGGTTTAGTAT
TGGTAGCGGGGGAAATTACGACTTCTACTTACGTAGATATTCCAAAAATCGTTCGTGAAACAATTCAAGGCATTGGTTAC
ACACGCGCAAAATACGGATTCGATGCAGAAACTTGTGCAGTTTTAACATCTATCGATGAGCAATCTGCTGACATCGCTAT
GGGTGTTGACCAAGCGCTAGAAGCACGCGAAGGTCAAATGACTGACGCTGAGATTGAGGCAATTGGTGCAGGAGACCAAG
GTTTAATGTTTGGTTTCGCATGTAATGAAACACAAGAGTTAATGCCACTTCCAATCTCGCTTGCTCACAAATTAGCTCGC
CGTTTAACTGAAGTACGTAAAAATGATACATTATCATACCTACGTCCGGATGGAAAAACGCAAGTTACAGTTGAGTATGA
TGAAAATGGTAAACCAGTACGTGTTGATACTATCGTAATTTCTACACAACATCACCCAGATGTTACATGGGAAGAAATCG
ATCGCGACTTAAAAGAGCACGTAATTAAAGCTGTAGTACCAGCAGAATTAATCGATGGAGAAACGAAATTCTTCATTAAC
CCAACTGGCCGCTTCGTAATTGGTGGACCACAAGGTGATGCTGGTTTAACAGGACGTAAAATCATCGTTGATACTTACGG
TGGATACGCTCGCCACGGTGGCGGTGCATTCTCTGGTAAAGATGCAACGAAAGTTGACCGTTCTGCAGCATATGCAGCTC
GTTATGTTGCGAAAAACATCGTAGCAGCTGGTCTTGCTGAAAAAGCAGAAGTACAACTTGCATACGCAATCGGTGTAGCA
CAACCAGTATCAATTTCAGTTGATACATTCGGCACTGGTAAAGTATCTGAAGACGTATTAGTAGAACTAGTTCGTAACAA
CTTCGATCTTCGCCCAGCTGGTATTATTAAAATGCTAGACTTACGTCGCCCAATTTACAAACAAACAGCAGCTTACGGTC
ACTTCGGACGTACTGATGTAGATCTATCATGGGAACGTACAGACAAAGCTGCAGCTTTAAAAGAGCAAGCTGGTCTATAA

Upstream 100 bases:

>100_bases
CTGTATGCTTGTGTGGGAGAATAAATGTATGTCGCAATCTGTGGCAAATTAAGGATGAGTTCCGTACAATATATACAATT
ACTGTAGGGAGGTTTACCAC

Downstream 100 bases:

>100_bases
TATATGAAAAAAAGCTTTGCGCAGTGAGCGCAAAGCTTTTTTATTTCGTTTTACTTACAATTTGAATGCATGAACTGCAA
TACACTTCGCCTTCTAGTTC

Product: S-adenosylmethionine synthetase

Products: NA

Alternate protein names: AdoMet synthase; MAT; Methionine adenosyltransferase

Number of amino acids: Translated: 399; Mature: 398

Protein sequence:

>399_residues
MTKKRHLFTSESVTEGHPDKICDQISDSILDAILSKDANARVACETTVTTGLVLVAGEITTSTYVDIPKIVRETIQGIGY
TRAKYGFDAETCAVLTSIDEQSADIAMGVDQALEAREGQMTDAEIEAIGAGDQGLMFGFACNETQELMPLPISLAHKLAR
RLTEVRKNDTLSYLRPDGKTQVTVEYDENGKPVRVDTIVISTQHHPDVTWEEIDRDLKEHVIKAVVPAELIDGETKFFIN
PTGRFVIGGPQGDAGLTGRKIIVDTYGGYARHGGGAFSGKDATKVDRSAAYAARYVAKNIVAAGLAEKAEVQLAYAIGVA
QPVSISVDTFGTGKVSEDVLVELVRNNFDLRPAGIIKMLDLRRPIYKQTAAYGHFGRTDVDLSWERTDKAAALKEQAGL

Sequences:

>Translated_399_residues
MTKKRHLFTSESVTEGHPDKICDQISDSILDAILSKDANARVACETTVTTGLVLVAGEITTSTYVDIPKIVRETIQGIGY
TRAKYGFDAETCAVLTSIDEQSADIAMGVDQALEAREGQMTDAEIEAIGAGDQGLMFGFACNETQELMPLPISLAHKLAR
RLTEVRKNDTLSYLRPDGKTQVTVEYDENGKPVRVDTIVISTQHHPDVTWEEIDRDLKEHVIKAVVPAELIDGETKFFIN
PTGRFVIGGPQGDAGLTGRKIIVDTYGGYARHGGGAFSGKDATKVDRSAAYAARYVAKNIVAAGLAEKAEVQLAYAIGVA
QPVSISVDTFGTGKVSEDVLVELVRNNFDLRPAGIIKMLDLRRPIYKQTAAYGHFGRTDVDLSWERTDKAAALKEQAGL
>Mature_398_residues
TKKRHLFTSESVTEGHPDKICDQISDSILDAILSKDANARVACETTVTTGLVLVAGEITTSTYVDIPKIVRETIQGIGYT
RAKYGFDAETCAVLTSIDEQSADIAMGVDQALEAREGQMTDAEIEAIGAGDQGLMFGFACNETQELMPLPISLAHKLARR
LTEVRKNDTLSYLRPDGKTQVTVEYDENGKPVRVDTIVISTQHHPDVTWEEIDRDLKEHVIKAVVPAELIDGETKFFINP
TGRFVIGGPQGDAGLTGRKIIVDTYGGYARHGGGAFSGKDATKVDRSAAYAARYVAKNIVAAGLAEKAEVQLAYAIGVAQ
PVSISVDTFGTGKVSEDVLVELVRNNFDLRPAGIIKMLDLRRPIYKQTAAYGHFGRTDVDLSWERTDKAAALKEQAGL

Specific function: Catalyzes the formation of S-adenosylmethionine from methionine and ATP. The overall synthetic reaction is composed of two sequential steps, AdoMet formation and the subsequent tripolyphosphate hydrolysis which occurs prior to release of AdoMet from the e

COG id: COG0192

COG function: function code H; S-adenosylmethionine synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AdoMet synthase family

Homologues:

Organism=Homo sapiens, GI4557737, Length=382, Percent_Identity=58.9005235602094, Blast_Score=435, Evalue=1e-122,
Organism=Homo sapiens, GI5174529, Length=382, Percent_Identity=59.4240837696335, Blast_Score=432, Evalue=1e-121,
Organism=Escherichia coli, GI1789311, Length=395, Percent_Identity=63.0379746835443, Blast_Score=501, Evalue=1e-143,
Organism=Caenorhabditis elegans, GI17538498, Length=389, Percent_Identity=55.5269922879177, Blast_Score=429, Evalue=1e-120,
Organism=Caenorhabditis elegans, GI25145633, Length=392, Percent_Identity=55.1020408163265, Blast_Score=427, Evalue=1e-120,
Organism=Caenorhabditis elegans, GI17538494, Length=389, Percent_Identity=54.7557840616967, Blast_Score=424, Evalue=1e-119,
Organism=Caenorhabditis elegans, GI17551082, Length=399, Percent_Identity=53.1328320802005, Blast_Score=412, Evalue=1e-115,
Organism=Caenorhabditis elegans, GI32565905, Length=389, Percent_Identity=50.3856041131105, Blast_Score=369, Evalue=1e-102,
Organism=Caenorhabditis elegans, GI32565911, Length=338, Percent_Identity=54.1420118343195, Blast_Score=355, Evalue=2e-98,
Organism=Caenorhabditis elegans, GI25145635, Length=341, Percent_Identity=53.3724340175953, Blast_Score=351, Evalue=4e-97,
Organism=Caenorhabditis elegans, GI32565903, Length=311, Percent_Identity=54.3408360128617, Blast_Score=326, Evalue=1e-89,
Organism=Caenorhabditis elegans, GI32565909, Length=87, Percent_Identity=52.8735632183908, Blast_Score=103, Evalue=1e-22,
Organism=Caenorhabditis elegans, GI32565907, Length=44, Percent_Identity=65.9090909090909, Blast_Score=71, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6323209, Length=383, Percent_Identity=55.8746736292428, Blast_Score=426, Evalue=1e-120,
Organism=Saccharomyces cerevisiae, GI6320710, Length=393, Percent_Identity=54.9618320610687, Blast_Score=425, Evalue=1e-120,
Organism=Drosophila melanogaster, GI45552159, Length=382, Percent_Identity=57.0680628272251, Blast_Score=407, Evalue=1e-114,
Organism=Drosophila melanogaster, GI24580529, Length=382, Percent_Identity=57.0680628272251, Blast_Score=407, Evalue=1e-114,
Organism=Drosophila melanogaster, GI24580537, Length=382, Percent_Identity=57.0680628272251, Blast_Score=407, Evalue=1e-114,
Organism=Drosophila melanogaster, GI24580533, Length=382, Percent_Identity=57.0680628272251, Blast_Score=407, Evalue=1e-114,
Organism=Drosophila melanogaster, GI24580535, Length=382, Percent_Identity=57.0680628272251, Blast_Score=407, Evalue=1e-114,
Organism=Drosophila melanogaster, GI24580531, Length=382, Percent_Identity=57.0680628272251, Blast_Score=407, Evalue=1e-114,
Organism=Drosophila melanogaster, GI24580527, Length=382, Percent_Identity=56.020942408377, Blast_Score=400, Evalue=1e-112,
Organism=Drosophila melanogaster, GI24580525, Length=382, Percent_Identity=56.020942408377, Blast_Score=400, Evalue=1e-112,
Organism=Drosophila melanogaster, GI24580541, Length=370, Percent_Identity=54.8648648648649, Blast_Score=379, Evalue=1e-105,
Organism=Drosophila melanogaster, GI24580539, Length=127, Percent_Identity=54.3307086614173, Blast_Score=135, Evalue=6e-32,

Paralogues:

None

Copy number: 7,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): METK_BACAA (C3PCC4)

Other databases:

- EMBL:   CP001598
- RefSeq:   YP_002869043.1
- ProteinModelPortal:   C3PCC4
- SMR:   C3PCC4
- EnsemblBacteria:   EBBACT00000126810
- GeneID:   7853489
- GenomeReviews:   CP001598_GR
- KEGG:   bai:BAA_5031
- GeneTree:   EBGT00050000002478
- ProtClustDB:   PRK05250
- GO:   GO:0005737
- HAMAP:   MF_00086
- InterPro:   IPR022631
- InterPro:   IPR022630
- InterPro:   IPR022629
- InterPro:   IPR022628
- InterPro:   IPR002133
- InterPro:   IPR022636
- PANTHER:   PTHR11964
- PIRSF:   PIRSF000497
- TIGRFAMs:   TIGR01034

Pfam domain/function: PF02773 S-AdoMet_synt_C; PF02772 S-AdoMet_synt_M; PF00438 S-AdoMet_synt_N; SSF55973 S-AdoMet_synt

EC number: =2.5.1.6

Molecular weight: Translated: 43263; Mature: 43132

Theoretical pI: Translated: 5.01; Mature: 5.01

Prosite motif: PS00376 ADOMET_SYNTHASE_1; PS00377 ADOMET_SYNTHASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKKRHLFTSESVTEGHPDKICDQISDSILDAILSKDANARVACETTVTTGLVLVAGEIT
CCCCCCEECCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEHHCCEEEEEECCC
TSTYVDIPKIVRETIQGIGYTRAKYGFDAETCAVLTSIDEQSADIAMGVDQALEAREGQM
CCHHCCHHHHHHHHHHHCCCCHHHCCCCHHHHHHHHHCCCCCCHHHHCHHHHHHHHCCCC
TDAEIEAIGAGDQGLMFGFACNETQELMPLPISLAHKLARRLTEVRKNDTLSYLRPDGKT
CCCCEEEECCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEECCCCCE
QVTVEYDENGKPVRVDTIVISTQHHPDVTWEEIDRDLKEHVIKAVVPAELIDGETKFFIN
EEEEEECCCCCEEEEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEC
PTGRFVIGGPQGDAGLTGRKIIVDTYGGYARHGGGAFSGKDATKVDRSAAYAARYVAKNI
CCCCEEEECCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
VAAGLAEKAEVQLAYAIGVAQPVSISVDTFGTGKVSEDVLVELVRNNFDLRPAGIIKMLD
HHHCCCCHHHEEEEEEECCCCCCEEEEECCCCCCCCHHHHHHHHHCCCCCCCHHHHHHHH
LRRPIYKQTAAYGHFGRTDVDLSWERTDKAAALKEQAGL
HCCHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHCCC
>Mature Secondary Structure 
TKKRHLFTSESVTEGHPDKICDQISDSILDAILSKDANARVACETTVTTGLVLVAGEIT
CCCCCEECCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEHHCCEEEEEECCC
TSTYVDIPKIVRETIQGIGYTRAKYGFDAETCAVLTSIDEQSADIAMGVDQALEAREGQM
CCHHCCHHHHHHHHHHHCCCCHHHCCCCHHHHHHHHHCCCCCCHHHHCHHHHHHHHCCCC
TDAEIEAIGAGDQGLMFGFACNETQELMPLPISLAHKLARRLTEVRKNDTLSYLRPDGKT
CCCCEEEECCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEECCCCCE
QVTVEYDENGKPVRVDTIVISTQHHPDVTWEEIDRDLKEHVIKAVVPAELIDGETKFFIN
EEEEEECCCCCEEEEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEC
PTGRFVIGGPQGDAGLTGRKIIVDTYGGYARHGGGAFSGKDATKVDRSAAYAARYVAKNI
CCCCEEEECCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
VAAGLAEKAEVQLAYAIGVAQPVSISVDTFGTGKVSEDVLVELVRNNFDLRPAGIIKMLD
HHHCCCCHHHEEEEEEECCCCCCEEEEECCCCCCCCHHHHHHHHHCCCCCCCHHHHHHHH
LRRPIYKQTAAYGHFGRTDVDLSWERTDKAAALKEQAGL
HCCHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA