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The map label for this gene is trmB

Identifier: 49187587

GI number: 49187587

Start: 4495414

End: 4496067

Strand: Reverse

Name: trmB

Synonym: BAS4594

Alternate gene names: 49187587

Gene position: 4496067-4495414 (Counterclockwise)

Preceding gene: 49187589

Following gene: 49187586

Centisome position: 85.99

GC content: 34.71

Gene sequence:

>654_bases
ATGCGTTTAAGACATAAACCATATGCAATGGATCGAATTAATGAGTATTCACATATCGTAATCGGAAACCCAGAGGAGCG
CGCTGGTAACTGGAAAGAAGTATTTGGAAATGAACAGCCAATTCACATTGAAGTAGGTACAGGTCGTGGCCGTTTTATGT
ATGATATGGCAAAAGCAAATCCTCATATTAATTATATTGGAATTGAAAAATTCACAAGTGTTGTTGTAGATGCACTTGAT
AAATTAATTGAAGAAGAATTACCGAACTTGAAATTAATTAATAAAGATGCTGAAGATTTAACAGTGTTCTTTGCAAAAGG
TGAGATTGATCGCGTTTATTTAAACTTCTCAGATCCATGGCCAAAGAAACGTCATACGAAGCGTCGTTTAACGTATAAAA
CGTTTTTACGCAATTATGAAGAAGTGTTAGTAGAAGGCGGAGAAATTCATTTCAAAACAGATAACCAAGGTTTATTTGAA
TATTCTCTTATGAGTATGGCTGAGTATGGTATGTTACTAACTTACTTAAGCTTAGATCTTCATAACAGCGACTTTGAAGG
TAATATTATGACTGAGTACGAAGAGAAATTCTCTAGTAAAGGTCATCGCATTTACCGAGTAGAAGCAAAATATCGTACAG
AGCCTATGCAGTAA

Upstream 100 bases:

>100_bases
GAGTGCTCGTACGTACTTGGTTTTTTGTTTGGTAGATTGATTAAAACAGAAAGAAGGAATTATTCCTTTAAAATAGCTAT
GTAAAGAGAGGGAAACATCT

Downstream 100 bases:

>100_bases
TGCATAGGTTTTTTGTATCTAAAATAATCGGAATTTTATGTTAGTATTGTAAGGAAAGGGGGATGAAAAATGGAACAGTT
ACAAATTGGAGATATAAAAG

Product: tRNA (guanine-N(7)-)-methyltransferase

Products: NA

Alternate protein names: tRNA(m7G46)-methyltransferase

Number of amino acids: Translated: 217; Mature: 217

Protein sequence:

>217_residues
MRLRHKPYAMDRINEYSHIVIGNPEERAGNWKEVFGNEQPIHIEVGTGRGRFMYDMAKANPHINYIGIEKFTSVVVDALD
KLIEEELPNLKLINKDAEDLTVFFAKGEIDRVYLNFSDPWPKKRHTKRRLTYKTFLRNYEEVLVEGGEIHFKTDNQGLFE
YSLMSMAEYGMLLTYLSLDLHNSDFEGNIMTEYEEKFSSKGHRIYRVEAKYRTEPMQ

Sequences:

>Translated_217_residues
MRLRHKPYAMDRINEYSHIVIGNPEERAGNWKEVFGNEQPIHIEVGTGRGRFMYDMAKANPHINYIGIEKFTSVVVDALD
KLIEEELPNLKLINKDAEDLTVFFAKGEIDRVYLNFSDPWPKKRHTKRRLTYKTFLRNYEEVLVEGGEIHFKTDNQGLFE
YSLMSMAEYGMLLTYLSLDLHNSDFEGNIMTEYEEKFSSKGHRIYRVEAKYRTEPMQ
>Mature_217_residues
MRLRHKPYAMDRINEYSHIVIGNPEERAGNWKEVFGNEQPIHIEVGTGRGRFMYDMAKANPHINYIGIEKFTSVVVDALD
KLIEEELPNLKLINKDAEDLTVFFAKGEIDRVYLNFSDPWPKKRHTKRRLTYKTFLRNYEEVLVEGGEIHFKTDNQGLFE
YSLMSMAEYGMLLTYLSLDLHNSDFEGNIMTEYEEKFSSKGHRIYRVEAKYRTEPMQ

Specific function: Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA

COG id: COG0220

COG function: function code R; Predicted S-adenosylmethionine-dependent methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. TrmB family

Homologues:

Organism=Escherichia coli, GI1789330, Length=176, Percent_Identity=32.3863636363636, Blast_Score=82, Evalue=3e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRMB_BACAA (C3PBG1)

Other databases:

- EMBL:   CP001598
- RefSeq:   YP_002868977.1
- ProteinModelPortal:   C3PBG1
- SMR:   C3PBG1
- EnsemblBacteria:   EBBACT00000125212
- GeneID:   7851430
- GenomeReviews:   CP001598_GR
- KEGG:   bai:BAA_4959
- GeneTree:   EBGT00050000000917
- ProtClustDB:   PRK00121
- HAMAP:   MF_01057
- InterPro:   IPR003358
- PANTHER:   PTHR23417:SF1
- TIGRFAMs:   TIGR00091

Pfam domain/function: PF02390 Methyltransf_4

EC number: =2.1.1.33

Molecular weight: Translated: 25600; Mature: 25600

Theoretical pI: Translated: 6.32; Mature: 6.32

Prosite motif: NA

Important sites: BINDING 44-44 BINDING 69-69 BINDING 96-96 BINDING 118-118 BINDING 122-122 BINDING 154-154

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLRHKPYAMDRINEYSHIVIGNPEERAGNWKEVFGNEQPIHIEVGTGRGRFMYDMAKAN
CCCCCCCCHHHHCCCCCEEEECCCHHHCCCHHHHCCCCCCEEEEEECCCCCEEEEEHHCC
PHINYIGIEKFTSVVVDALDKLIEEELPNLKLINKDAEDLTVFFAKGEIDRVYLNFSDPW
CCEEEEEHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCEEEEEECCCEEEEEEECCCCC
PKKRHTKRRLTYKTFLRNYEEVLVEGGEIHFKTDNQGLFEYSLMSMAEYGMLLTYLSLDL
CCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHEEEC
HNSDFEGNIMTEYEEKFSSKGHRIYRVEAKYRTEPMQ
CCCCCCCCEEHHHHHHHCCCCCEEEEEEEECCCCCCC
>Mature Secondary Structure
MRLRHKPYAMDRINEYSHIVIGNPEERAGNWKEVFGNEQPIHIEVGTGRGRFMYDMAKAN
CCCCCCCCHHHHCCCCCEEEECCCHHHCCCHHHHCCCCCCEEEEEECCCCCEEEEEHHCC
PHINYIGIEKFTSVVVDALDKLIEEELPNLKLINKDAEDLTVFFAKGEIDRVYLNFSDPW
CCEEEEEHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCEEEEEECCCEEEEEEECCCCC
PKKRHTKRRLTYKTFLRNYEEVLVEGGEIHFKTDNQGLFEYSLMSMAEYGMLLTYLSLDL
CCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHEEEC
HNSDFEGNIMTEYEEKFSSKGHRIYRVEAKYRTEPMQ
CCCCCCCCEEHHHHHHHCCCCCEEEEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA