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The map label for this gene is ppnK

Identifier: 49187534

GI number: 49187534

Start: 4445710

End: 4446513

Strand: Direct

Name: ppnK

Synonym: BAS4540

Alternate gene names: 49187534

Gene position: 4445710-4446513 (Clockwise)

Preceding gene: 49187528

Following gene: 49187543

Centisome position: 85.03

GC content: 35.32

Gene sequence:

>804_bases
ATGGCAGATCGTCGCAATTTATTTTTCTTTTATGGTGATGACAAAGCAAAGCTTGTTGAAAAAATGAAACCAATCTATCG
TATTTTAGAAGAGAATGGATTCACCATATTAGATCATCCAAAAAATGCAAACGCTATCGTCAGTGTTGGAGATGATGCAA
CTTTCTTACAAGCCGTTCGTAAAACTGGTTTTAGGGAAGATTGCTTATACGCAGGGATTTCTACGAAAGATGAAATTTCA
TTCTACTGCGATTTCCATATTGATCACGTTGATACAGCCCTTCAAGAAATTACAAAAAATGAAATTGAAGTGCGAAAATA
TCCAACAATTGAGGTAGATGTAGATGGTAGCACATCTTTCCATTGTTTAAATGAGTTCTCGTTACGTTCTAGCATTATTA
AAACGTTCGTTGTAGATGTTCACGTTGATGATTTATATTTCGAAACTTTTAGAGGTGACGGTTTAGTTGTTTCTACCCCA
ACAGGAAGTACAGCTTACAATAAATCGTTACGTGGTGCAGTTGTTGACCCGCTTATCCCTTGCTTCCAAGTAAGTGAATT
GGCATCTTTAAATAACAACACATATCGTACACTTGGTTCACCGTTCATTTTAAATCACGAACGTACATTAACTTTAAAAC
TAAGACCAGACGGTAACGATTATCCTGTTATCGGTATGGATAACGAAGCGCTTAGCATTAAACAAGTGGAAAAAGCTGTT
GTACGCTTAAGCGATAAACAAATTAAAACAGTTAAATTAAAAAACAACTCTTTCTGGGAAAAAGTACAGAGAACGTTTTT
ATAG

Upstream 100 bases:

>100_bases
TTATATCGAAATATATTATTTGAACATATCGTCAGAGAACGGACTCTAATGATATGTAGCACGAAGCATAGAAAATTTAA
CTGCAGTGGAGGGATACATA

Downstream 100 bases:

>100_bases
TATTAAAATAACCGTAGAATTATGGTGGCCCATAATTCTACGGTTATTTATTTACCCCGATTGGCGCGAACAACAACCTC
TCGCTATCCGGCCAATATTC

Product: inorganic polyphosphate/ATP-NAD kinase

Products: NA

Alternate protein names: Poly(P)/ATP NAD kinase 2

Number of amino acids: Translated: 267; Mature: 266

Protein sequence:

>267_residues
MADRRNLFFFYGDDKAKLVEKMKPIYRILEENGFTILDHPKNANAIVSVGDDATFLQAVRKTGFREDCLYAGISTKDEIS
FYCDFHIDHVDTALQEITKNEIEVRKYPTIEVDVDGSTSFHCLNEFSLRSSIIKTFVVDVHVDDLYFETFRGDGLVVSTP
TGSTAYNKSLRGAVVDPLIPCFQVSELASLNNNTYRTLGSPFILNHERTLTLKLRPDGNDYPVIGMDNEALSIKQVEKAV
VRLSDKQIKTVKLKNNSFWEKVQRTFL

Sequences:

>Translated_267_residues
MADRRNLFFFYGDDKAKLVEKMKPIYRILEENGFTILDHPKNANAIVSVGDDATFLQAVRKTGFREDCLYAGISTKDEIS
FYCDFHIDHVDTALQEITKNEIEVRKYPTIEVDVDGSTSFHCLNEFSLRSSIIKTFVVDVHVDDLYFETFRGDGLVVSTP
TGSTAYNKSLRGAVVDPLIPCFQVSELASLNNNTYRTLGSPFILNHERTLTLKLRPDGNDYPVIGMDNEALSIKQVEKAV
VRLSDKQIKTVKLKNNSFWEKVQRTFL
>Mature_266_residues
ADRRNLFFFYGDDKAKLVEKMKPIYRILEENGFTILDHPKNANAIVSVGDDATFLQAVRKTGFREDCLYAGISTKDEISF
YCDFHIDHVDTALQEITKNEIEVRKYPTIEVDVDGSTSFHCLNEFSLRSSIIKTFVVDVHVDDLYFETFRGDGLVVSTPT
GSTAYNKSLRGAVVDPLIPCFQVSELASLNNNTYRTLGSPFILNHERTLTLKLRPDGNDYPVIGMDNEALSIKQVEKAVV
RLSDKQIKTVKLKNNSFWEKVQRTFL

Specific function: Catalyzes the phosphorylation of NAD to NADP. Utilizes ATP and other nucleoside triphosphates as well as inorganic polyphosphate as a source of phosphorus

COG id: COG0061

COG function: function code G; Predicted sugar kinase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the NAD kinase family

Homologues:

Organism=Escherichia coli, GI1788968, Length=229, Percent_Identity=26.6375545851528, Blast_Score=66, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PPNK2_BACAN (Q81KU5)

Other databases:

- EMBL:   AE016879
- EMBL:   AE017334
- EMBL:   AE017225
- RefSeq:   NP_847093.1
- RefSeq:   YP_021537.1
- RefSeq:   YP_030787.1
- ProteinModelPortal:   Q81KU5
- SMR:   Q81KU5
- EnsemblBacteria:   EBBACT00000011978
- EnsemblBacteria:   EBBACT00000014062
- EnsemblBacteria:   EBBACT00000021014
- GeneID:   1086739
- GeneID:   2819564
- GeneID:   2850103
- GenomeReviews:   AE016879_GR
- GenomeReviews:   AE017225_GR
- GenomeReviews:   AE017334_GR
- KEGG:   ban:BA_4893
- KEGG:   bar:GBAA_4893
- KEGG:   bat:BAS4540
- TIGR:   BA_4893
- TIGR:   GBAA_4893
- GeneTree:   EBGT00050000002705
- HOGENOM:   HBG314020
- OMA:   NDYPIIG
- ProtClustDB:   PRK03501
- BioCyc:   BANT260799:BAS4540-MONOMER
- BioCyc:   BANT261594:GBAA4893-MONOMER
- BRENDA:   2.7.1.23
- GO:   GO:0005737
- HAMAP:   MF_00361
- InterPro:   IPR016064
- InterPro:   IPR017438
- InterPro:   IPR017437
- InterPro:   IPR002504
- Gene3D:   G3DSA:2.60.200.30
- Gene3D:   G3DSA:3.40.50.10330
- PANTHER:   PTHR20275

Pfam domain/function: PF01513 NAD_kinase; SSF111331 ATP-NAD_kinase_PpnK-typ

EC number: =2.7.1.23

Molecular weight: Translated: 30365; Mature: 30234

Theoretical pI: Translated: 5.98; Mature: 5.98

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MADRRNLFFFYGDDKAKLVEKMKPIYRILEENGFTILDHPKNANAIVSVGDDATFLQAVR
CCCCCEEEEEECCCHHHHHHHHHHHHHHHHCCCCEEEECCCCCCEEEEECCCHHHHHHHH
KTGFREDCLYAGISTKDEISFYCDFHIDHVDTALQEITKNEIEVRKYPTIEVDVDGSTSF
HCCCCCHHEEECCCCCCCEEEEEEEECHHHHHHHHHHHCCCEEEEECCEEEEEECCCCCE
HCLNEFSLRSSIIKTFVVDVHVDDLYFETFRGDGLVVSTPTGSTAYNKSLRGAVVDPLIP
EEHHHHHHHHHHHHHEEEEEEECCEEEEEECCCEEEEECCCCCCHHCCCCCCCHHHHHHH
CFQVSELASLNNNTYRTLGSPFILNHERTLTLKLRPDGNDYPVIGMDNEALSIKQVEKAV
HHHHHHHHHCCCCCEEECCCCEEEECCEEEEEEECCCCCCCCEEECCCCCHHHHHHHHHH
VRLSDKQIKTVKLKNNSFWEKVQRTFL
HHCCCCEEEEEEECCCHHHHHHHHHCC
>Mature Secondary Structure 
ADRRNLFFFYGDDKAKLVEKMKPIYRILEENGFTILDHPKNANAIVSVGDDATFLQAVR
CCCCEEEEEECCCHHHHHHHHHHHHHHHHCCCCEEEECCCCCCEEEEECCCHHHHHHHH
KTGFREDCLYAGISTKDEISFYCDFHIDHVDTALQEITKNEIEVRKYPTIEVDVDGSTSF
HCCCCCHHEEECCCCCCCEEEEEEEECHHHHHHHHHHHCCCEEEEECCEEEEEECCCCCE
HCLNEFSLRSSIIKTFVVDVHVDDLYFETFRGDGLVVSTPTGSTAYNKSLRGAVVDPLIP
EEHHHHHHHHHHHHHEEEEEEECCEEEEEECCCEEEEECCCCCCHHCCCCCCCHHHHHHH
CFQVSELASLNNNTYRTLGSPFILNHERTLTLKLRPDGNDYPVIGMDNEALSIKQVEKAV
HHHHHHHHHCCCCCEEECCCCEEEECCEEEEEEECCCCCCCCEEECCCCCHHHHHHHHHH
VRLSDKQIKTVKLKNNSFWEKVQRTFL
HHCCCCEEEEEEECCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12721629