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The map label for this gene is citZ [H]

Identifier: 49187483

GI number: 49187483

Start: 4402167

End: 4403282

Strand: Reverse

Name: citZ [H]

Synonym: BAS4488

Alternate gene names: 49187483

Gene position: 4403282-4402167 (Counterclockwise)

Preceding gene: 49187484

Following gene: 49187482

Centisome position: 84.21

GC content: 36.65

Gene sequence:

>1116_bases
ATGACTGTTATTCGAGGTTTAGAAGGGGTAGTAGCAACAACATCATCTGTGAGCTCTATTATTGATGATACATTAACTTA
TGCTGGGTATAATATTGATGATTTAGCTGAGAATGCTACATTTGAAGAAGTAGTGTACTTATTATGGCATCGCAAGCTTC
CTAACGAGCAAGAACTAGCGAAATTTAATGAGATTGTATCGGAATACTATAAAGTTCCAGGTGAGATTTTAGCATATTTG
AAACAAGTAGATTTAAAAATCGCACATCCGATGTCTGTTTTACGAACTGCAATTTCCATGCTATCATTATACGATGAGAG
CGCTGAAATTATGGATGAAAAGTCCAATTATTTGAAAGCGGTTAAATTACAGGCTCAAGTGGGTACTTTAGTTGCTGCTT
ATGCAAGAATCCGTAAAGGTTTAGATGTTGTTGAGCCTAGAAATGATTTATCATTAGCTGCAAACTTCTTGTACATGTTA
AATGATCGCGAGCCAAATGAAGTTGAAATTGAAGCTTTTGATAAGGCGCTTGTACTTCACGCAGATCATGAGTTAAACGC
TTCTACATTTACTGCACGCGTTTGTGTAGCTACACTTTCAGATGTATATTCTGGTATTACAGCAGCAATCGGTGCGTTAA
AAGGTCCTCTTCACGGCGGGGCAAATGAAAATGTAATGAAGATGCTAACTGAAATTGGCGAAGAAGAAAATGTAGAATCA
TATATTCATAATGCTCTTCAAAATAAAGTGAAAATCATGGGCTTTGGCCACCGTGTATATGAGCAGGGTGATCCGCGTGC
AAAACATTTACGCGAAATGTCTAAGAGATTATGCGTGCTTTTAGGAGAAGAGAAATGGTATAATATGTCTATCAAAATTG
AAGACATTGTAACAAAAGAAAAAGGTCTTCCACCAAATGTTGATTTCTATTCAGCTTCTGTATACCATTGTTTAGGAATT
GACCATGACTTATTTACACCTATTTTTGCAATTAGCCGTATGTCAGGCTGGTTAGCTCATATTCTAGAACAATATGAAAA
TAACCGTTTAATCCGTCCGCGTGCTGATTATAATGGACCAACACATCAAGTGTATGTTCCAATTGCACAACGATAA

Upstream 100 bases:

>100_bases
TGCTTGCCTAAAGAGTGAACGGGCGTTCATAATTCAAAAGGGGAGCAAATAGATATAAGGAGCAAGGTTGGGAGAATTTT
CAGGAAAAGGAGAGAATGTC

Downstream 100 bases:

>100_bases
GCGAATAACACTATTTTGATAGTGAAAATACGCTTTCAAAAGTCTGATTTTTTCGGAAAGATGTAATGATTAGAATATTT
TAATTTGACTAACGGTTTGA

Product: citrate synthase

Products: NA

Alternate protein names: Citrate synthase II [H]

Number of amino acids: Translated: 371; Mature: 370

Protein sequence:

>371_residues
MTVIRGLEGVVATTSSVSSIIDDTLTYAGYNIDDLAENATFEEVVYLLWHRKLPNEQELAKFNEIVSEYYKVPGEILAYL
KQVDLKIAHPMSVLRTAISMLSLYDESAEIMDEKSNYLKAVKLQAQVGTLVAAYARIRKGLDVVEPRNDLSLAANFLYML
NDREPNEVEIEAFDKALVLHADHELNASTFTARVCVATLSDVYSGITAAIGALKGPLHGGANENVMKMLTEIGEEENVES
YIHNALQNKVKIMGFGHRVYEQGDPRAKHLREMSKRLCVLLGEEKWYNMSIKIEDIVTKEKGLPPNVDFYSASVYHCLGI
DHDLFTPIFAISRMSGWLAHILEQYENNRLIRPRADYNGPTHQVYVPIAQR

Sequences:

>Translated_371_residues
MTVIRGLEGVVATTSSVSSIIDDTLTYAGYNIDDLAENATFEEVVYLLWHRKLPNEQELAKFNEIVSEYYKVPGEILAYL
KQVDLKIAHPMSVLRTAISMLSLYDESAEIMDEKSNYLKAVKLQAQVGTLVAAYARIRKGLDVVEPRNDLSLAANFLYML
NDREPNEVEIEAFDKALVLHADHELNASTFTARVCVATLSDVYSGITAAIGALKGPLHGGANENVMKMLTEIGEEENVES
YIHNALQNKVKIMGFGHRVYEQGDPRAKHLREMSKRLCVLLGEEKWYNMSIKIEDIVTKEKGLPPNVDFYSASVYHCLGI
DHDLFTPIFAISRMSGWLAHILEQYENNRLIRPRADYNGPTHQVYVPIAQR
>Mature_370_residues
TVIRGLEGVVATTSSVSSIIDDTLTYAGYNIDDLAENATFEEVVYLLWHRKLPNEQELAKFNEIVSEYYKVPGEILAYLK
QVDLKIAHPMSVLRTAISMLSLYDESAEIMDEKSNYLKAVKLQAQVGTLVAAYARIRKGLDVVEPRNDLSLAANFLYMLN
DREPNEVEIEAFDKALVLHADHELNASTFTARVCVATLSDVYSGITAAIGALKGPLHGGANENVMKMLTEIGEEENVESY
IHNALQNKVKIMGFGHRVYEQGDPRAKHLREMSKRLCVLLGEEKWYNMSIKIEDIVTKEKGLPPNVDFYSASVYHCLGID
HDLFTPIFAISRMSGWLAHILEQYENNRLIRPRADYNGPTHQVYVPIAQR

Specific function: Might regulate the synthesis and function of enzymes involved in later enzymatic steps of Krebs cycle. Loss in activity results in sporulation defect [H]

COG id: COG0372

COG function: function code C; Citrate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the citrate synthase family [H]

Homologues:

Organism=Homo sapiens, GI38327625, Length=374, Percent_Identity=25.668449197861, Blast_Score=102, Evalue=7e-22,
Organism=Escherichia coli, GI1786527, Length=368, Percent_Identity=35.8695652173913, Blast_Score=238, Evalue=6e-64,
Organism=Escherichia coli, GI1786939, Length=371, Percent_Identity=33.9622641509434, Blast_Score=202, Evalue=2e-53,
Organism=Caenorhabditis elegans, GI17555174, Length=373, Percent_Identity=27.0777479892761, Blast_Score=99, Evalue=3e-21,
Organism=Saccharomyces cerevisiae, GI6319850, Length=377, Percent_Identity=23.0769230769231, Blast_Score=81, Evalue=3e-16,
Organism=Saccharomyces cerevisiae, GI6324328, Length=386, Percent_Identity=22.7979274611399, Blast_Score=75, Evalue=1e-14,
Organism=Saccharomyces cerevisiae, GI6325257, Length=180, Percent_Identity=31.1111111111111, Blast_Score=74, Evalue=4e-14,
Organism=Drosophila melanogaster, GI24640126, Length=377, Percent_Identity=27.0557029177719, Blast_Score=99, Evalue=5e-21,
Organism=Drosophila melanogaster, GI24640124, Length=377, Percent_Identity=27.0557029177719, Blast_Score=99, Evalue=6e-21,
Organism=Drosophila melanogaster, GI21356863, Length=377, Percent_Identity=27.3209549071618, Blast_Score=92, Evalue=6e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011278
- InterPro:   IPR016142
- InterPro:   IPR016143
- InterPro:   IPR002020
- InterPro:   IPR016141
- InterPro:   IPR019810 [H]

Pfam domain/function: PF00285 Citrate_synt [H]

EC number: =2.3.3.1 [H]

Molecular weight: Translated: 41784; Mature: 41653

Theoretical pI: Translated: 5.29; Mature: 5.29

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTVIRGLEGVVATTSSVSSIIDDTLTYAGYNIDDLAENATFEEVVYLLWHRKLPNEQELA
CCCHHCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCHHHHHHHHHHHCCCCHHHHH
KFNEIVSEYYKVPGEILAYLKQVDLKIAHPMSVLRTAISMLSLYDESAEIMDEKSNYLKA
HHHHHHHHHHHCCHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VKLQAQVGTLVAAYARIRKGLDVVEPRNDLSLAANFLYMLNDREPNEVEIEAFDKALVLH
HHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHEECCCCCCEEEHEECCCEEEEE
ADHELNASTFTARVCVATLSDVYSGITAAIGALKGPLHGGANENVMKMLTEIGEEENVES
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCCCCHHH
YIHNALQNKVKIMGFGHRVYEQGDPRAKHLREMSKRLCVLLGEEKWYNMSIKIEDIVTKE
HHHHHHHCCEEEEECCHHHHHCCCCHHHHHHHHHHHHHEEECCCCEEEEEEEEHHHHHHC
KGLPPNVDFYSASVYHCLGIDHDLFTPIFAISRMSGWLAHILEQYENNRLIRPRADYNGP
CCCCCCCCCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEECCCCCCCCC
THQVYVPIAQR
CEEEEEEECCC
>Mature Secondary Structure 
TVIRGLEGVVATTSSVSSIIDDTLTYAGYNIDDLAENATFEEVVYLLWHRKLPNEQELA
CCHHCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCHHHHHHHHHHHCCCCHHHHH
KFNEIVSEYYKVPGEILAYLKQVDLKIAHPMSVLRTAISMLSLYDESAEIMDEKSNYLKA
HHHHHHHHHHHCCHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VKLQAQVGTLVAAYARIRKGLDVVEPRNDLSLAANFLYMLNDREPNEVEIEAFDKALVLH
HHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHEECCCCCCEEEHEECCCEEEEE
ADHELNASTFTARVCVATLSDVYSGITAAIGALKGPLHGGANENVMKMLTEIGEEENVES
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCCCCHHH
YIHNALQNKVKIMGFGHRVYEQGDPRAKHLREMSKRLCVLLGEEKWYNMSIKIEDIVTKE
HHHHHHHCCEEEEECCHHHHHCCCCHHHHHHHHHHHHHEEECCCCEEEEEEEEHHHHHHC
KGLPPNVDFYSASVYHCLGIDHDLFTPIFAISRMSGWLAHILEQYENNRLIRPRADYNGP
CCCCCCCCCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEECCCCCCCCC
THQVYVPIAQR
CEEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8045898; 9387221; 9384377 [H]