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The map label for this gene is mdh

Identifier: 49187481

GI number: 49187481

Start: 4399756

End: 4400694

Strand: Reverse

Name: mdh

Synonym: BAS4486

Alternate gene names: 49187481

Gene position: 4400694-4399756 (Counterclockwise)

Preceding gene: 49187482

Following gene: 49187480

Centisome position: 84.16

GC content: 39.94

Gene sequence:

>939_bases
ATGACAATCAAACGCAAGAAAGTTTCAGTCATCGGTGCAGGATTTACAGGAGCAACAACAGCATTCTTATTAGCACAAAA
AGAACTTGCAGATGTTGTATTAGTGGACATTCCGCAGCTGGAAAATCCAACAAAAGGGAAAGCGTTAGATATGTTAGAAG
CGAGCCCAGTACAAGGTTTTGATGCTAACATTATTGGAACATCTGATTACGCAGATACTGCTGATTCTGACGTTGTTGTG
ATTACAGCAGGTATTGCGCGTAAGCCTGGTATGAGCCGTGATGATTTAGTAGCAACAAACTCCAAAATTATGAAAAGTAT
TACGCGCGACATTGCAAAACATTCACCAAATGCGATTATTGTTGTATTAACAAATCCAGTTGATGCAATGACATATTCTG
TATTTAAAGAAGCAGGATTCCCGAAAGAGCGTGTTATCGGTCAATCGGGCGTATTAGATACAGCTCGTTTCCGTACATTC
ATCGCACAAGAATTAAATCTTTCTGTGAAAGACATTACAGGATTTGTTCTTGGTGGACACGGTGACGATATGGTACCTCT
TGTACGTTATTCATATGCAGGTGGCATTCCGTTAGAAACGTTAATTCCGAAAGAGCGTTTAGAAGCAATCGTAGAACGTA
CCCGTAAAGGTGGCGGCGAGATTGTAGGCTTATTAGGAAACGGTAGTGCATATTACGCACCAGCTGCTTCTTTAGTTGAA
ATGACAGAAGCAATCTTAAAAGATCAACGCCGTGTATTACCGGCTATTGCGTACCTTGAAGGTGAATACGGTTATAGTGA
CCTTTACTTAGGGGTACCAGTAATTCTAGGTGGTAACGGAATTGAAAAAATTATTGAATTAGAACTTCTTGCAGATGAAA
AAGAAGCGTTAGATCGCTCTGTAGAATCTGTACGTAATGTTATGAAGGTTCTTGTTTAA

Upstream 100 bases:

>100_bases
GGTGCAACAGAAGTGAAATGTTCTGAGTTCGCTAATGAACTTATTAAAAACATGGATGTAGCGATAATCAAAAACGCATA
ATTAAAGCGGGGGGTAAATT

Downstream 100 bases:

>100_bases
TCATTAAATATAAGTAGAAAAAGATTCGGGGCCCCGAATCTTTTTCTACTATTTATACAAATGTTTTTTTGAAATAACAT
GAAAACGTTATCATTTGTAA

Product: malate dehydrogenase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 312; Mature: 311

Protein sequence:

>312_residues
MTIKRKKVSVIGAGFTGATTAFLLAQKELADVVLVDIPQLENPTKGKALDMLEASPVQGFDANIIGTSDYADTADSDVVV
ITAGIARKPGMSRDDLVATNSKIMKSITRDIAKHSPNAIIVVLTNPVDAMTYSVFKEAGFPKERVIGQSGVLDTARFRTF
IAQELNLSVKDITGFVLGGHGDDMVPLVRYSYAGGIPLETLIPKERLEAIVERTRKGGGEIVGLLGNGSAYYAPAASLVE
MTEAILKDQRRVLPAIAYLEGEYGYSDLYLGVPVILGGNGIEKIIELELLADEKEALDRSVESVRNVMKVLV

Sequences:

>Translated_312_residues
MTIKRKKVSVIGAGFTGATTAFLLAQKELADVVLVDIPQLENPTKGKALDMLEASPVQGFDANIIGTSDYADTADSDVVV
ITAGIARKPGMSRDDLVATNSKIMKSITRDIAKHSPNAIIVVLTNPVDAMTYSVFKEAGFPKERVIGQSGVLDTARFRTF
IAQELNLSVKDITGFVLGGHGDDMVPLVRYSYAGGIPLETLIPKERLEAIVERTRKGGGEIVGLLGNGSAYYAPAASLVE
MTEAILKDQRRVLPAIAYLEGEYGYSDLYLGVPVILGGNGIEKIIELELLADEKEALDRSVESVRNVMKVLV
>Mature_311_residues
TIKRKKVSVIGAGFTGATTAFLLAQKELADVVLVDIPQLENPTKGKALDMLEASPVQGFDANIIGTSDYADTADSDVVVI
TAGIARKPGMSRDDLVATNSKIMKSITRDIAKHSPNAIIVVLTNPVDAMTYSVFKEAGFPKERVIGQSGVLDTARFRTFI
AQELNLSVKDITGFVLGGHGDDMVPLVRYSYAGGIPLETLIPKERLEAIVERTRKGGGEIVGLLGNGSAYYAPAASLVEM
TEAILKDQRRVLPAIAYLEGEYGYSDLYLGVPVILGGNGIEKIIELELLADEKEALDRSVESVRNVMKVLV

Specific function: Catalyzes the reversible oxidation of malate to oxaloacetate

COG id: COG0039

COG function: function code C; Malate/lactate dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the LDH/MDH superfamily. MDH type 3 family

Homologues:

Organism=Homo sapiens, GI47059044, Length=318, Percent_Identity=38.0503144654088, Blast_Score=208, Evalue=4e-54,
Organism=Homo sapiens, GI221136809, Length=318, Percent_Identity=38.0503144654088, Blast_Score=208, Evalue=4e-54,
Organism=Homo sapiens, GI260099723, Length=316, Percent_Identity=35.4430379746835, Blast_Score=189, Evalue=3e-48,
Organism=Homo sapiens, GI5031857, Length=316, Percent_Identity=35.4430379746835, Blast_Score=189, Evalue=4e-48,
Organism=Homo sapiens, GI15082234, Length=318, Percent_Identity=34.2767295597484, Blast_Score=187, Evalue=1e-47,
Organism=Homo sapiens, GI9257228, Length=315, Percent_Identity=34.6031746031746, Blast_Score=179, Evalue=3e-45,
Organism=Homo sapiens, GI4504973, Length=315, Percent_Identity=34.6031746031746, Blast_Score=179, Evalue=3e-45,
Organism=Homo sapiens, GI291575128, Length=319, Percent_Identity=33.2288401253918, Blast_Score=176, Evalue=3e-44,
Organism=Homo sapiens, GI4557032, Length=319, Percent_Identity=33.2288401253918, Blast_Score=176, Evalue=3e-44,
Organism=Homo sapiens, GI260099727, Length=221, Percent_Identity=37.5565610859729, Blast_Score=139, Evalue=3e-33,
Organism=Homo sapiens, GI260099725, Length=200, Percent_Identity=39, Blast_Score=139, Evalue=4e-33,
Organism=Homo sapiens, GI207028494, Length=198, Percent_Identity=34.3434343434343, Blast_Score=121, Evalue=1e-27,
Organism=Homo sapiens, GI21735621, Length=328, Percent_Identity=30.1829268292683, Blast_Score=107, Evalue=1e-23,
Organism=Homo sapiens, GI103472011, Length=310, Percent_Identity=25.4838709677419, Blast_Score=96, Evalue=3e-20,
Organism=Homo sapiens, GI103472015, Length=182, Percent_Identity=28.5714285714286, Blast_Score=71, Evalue=1e-12,
Organism=Escherichia coli, GI1789632, Length=316, Percent_Identity=31.3291139240506, Blast_Score=98, Evalue=7e-22,
Organism=Caenorhabditis elegans, GI17535107, Length=303, Percent_Identity=30.6930693069307, Blast_Score=145, Evalue=3e-35,
Organism=Caenorhabditis elegans, GI17554310, Length=316, Percent_Identity=31.9620253164557, Blast_Score=108, Evalue=4e-24,
Organism=Saccharomyces cerevisiae, GI6322765, Length=295, Percent_Identity=30.8474576271186, Blast_Score=93, Evalue=4e-20,
Organism=Saccharomyces cerevisiae, GI6320125, Length=239, Percent_Identity=30.9623430962343, Blast_Score=80, Evalue=4e-16,
Organism=Drosophila melanogaster, GI17136226, Length=313, Percent_Identity=31.3099041533546, Blast_Score=159, Evalue=3e-39,
Organism=Drosophila melanogaster, GI45550422, Length=302, Percent_Identity=31.1258278145695, Blast_Score=125, Evalue=4e-29,
Organism=Drosophila melanogaster, GI24647881, Length=319, Percent_Identity=31.6614420062696, Blast_Score=112, Evalue=3e-25,
Organism=Drosophila melanogaster, GI24663599, Length=311, Percent_Identity=28.2958199356913, Blast_Score=89, Evalue=5e-18,
Organism=Drosophila melanogaster, GI24663595, Length=242, Percent_Identity=29.7520661157025, Blast_Score=88, Evalue=7e-18,

Paralogues:

None

Copy number: 2640 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 260 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2380 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). 260 Molecules/Cell In: Stationary Phase

Swissprot (AC and ID): MDH_BACAA (C3PAI1)

Other databases:

- EMBL:   CP001598
- RefSeq:   YP_002868871.1
- ProteinModelPortal:   C3PAI1
- SMR:   C3PAI1
- EnsemblBacteria:   EBBACT00000126290
- GeneID:   7850458
- GenomeReviews:   CP001598_GR
- KEGG:   bai:BAA_4848
- GeneTree:   EBGT00070000032140
- ProtClustDB:   PRK06223
- GO:   GO:0005488
- GO:   GO:0006096
- HAMAP:   MF_00487
- InterPro:   IPR001557
- InterPro:   IPR022383
- InterPro:   IPR001236
- InterPro:   IPR015955
- InterPro:   IPR011275
- InterPro:   IPR016040
- Gene3D:   G3DSA:3.90.110.10
- Gene3D:   G3DSA:3.40.50.720
- PIRSF:   PIRSF000102
- PRINTS:   PR00086
- TIGRFAMs:   TIGR01763

Pfam domain/function: PF02866 Ldh_1_C; PF00056 Ldh_1_N; SSF56327 Lactate_DH/Glyco_hydro_4_C

EC number: =1.1.1.37

Molecular weight: Translated: 33498; Mature: 33367

Theoretical pI: Translated: 4.87; Mature: 4.87

Prosite motif: NA

Important sites: ACT_SITE 180-180 BINDING 36-36 BINDING 87-87 BINDING 93-93 BINDING 100-100 BINDING 125-125 BINDING 156-156

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTIKRKKVSVIGAGFTGATTAFLLAQKELADVVLVDIPQLENPTKGKALDMLEASPVQGF
CCCCCEEEEEEECCCCHHHHHHHHHHHHHHCEEEEECCCCCCCCCCCHHHHHCCCCCCCC
DANIIGTSDYADTADSDVVVITAGIARKPGMSRDDLVATNSKIMKSITRDIAKHSPNAII
CCEEEECCCCCCCCCCCEEEEEECCCCCCCCCCCCEEHHHHHHHHHHHHHHHHCCCCEEE
VVLTNPVDAMTYSVFKEAGFPKERVIGQSGVLDTARFRTFIAQELNLSVKDITGFVLGGH
EEEECCHHHHHHHHHHHCCCCHHHHCCCCCCCHHHHHHHHHHHHHCCCHHHEEEEEECCC
GDDMVPLVRYSYAGGIPLETLIPKERLEAIVERTRKGGGEIVGLLGNGSAYYAPAASLVE
CCCCHHHHHHHCCCCCCHHHCCCHHHHHHHHHHHHCCCCEEEEEEECCCEEECCHHHHHH
MTEAILKDQRRVLPAIAYLEGEYGYSDLYLGVPVILGGNGIEKIIELELLADEKEALDRS
HHHHHHHHHHHHHHHHHEEECCCCCCEEEECCEEEECCCCHHHHHHHHHHCCHHHHHHHH
VESVRNVMKVLV
HHHHHHHHHHHC
>Mature Secondary Structure 
TIKRKKVSVIGAGFTGATTAFLLAQKELADVVLVDIPQLENPTKGKALDMLEASPVQGF
CCCCEEEEEEECCCCHHHHHHHHHHHHHHCEEEEECCCCCCCCCCCHHHHHCCCCCCCC
DANIIGTSDYADTADSDVVVITAGIARKPGMSRDDLVATNSKIMKSITRDIAKHSPNAII
CCEEEECCCCCCCCCCCEEEEEECCCCCCCCCCCCEEHHHHHHHHHHHHHHHHCCCCEEE
VVLTNPVDAMTYSVFKEAGFPKERVIGQSGVLDTARFRTFIAQELNLSVKDITGFVLGGH
EEEECCHHHHHHHHHHHCCCCHHHHCCCCCCCHHHHHHHHHHHHHCCCHHHEEEEEECCC
GDDMVPLVRYSYAGGIPLETLIPKERLEAIVERTRKGGGEIVGLLGNGSAYYAPAASLVE
CCCCHHHHHHHCCCCCCHHHCCCHHHHHHHHHHHHCCCCEEEEEEECCCEEECCHHHHHH
MTEAILKDQRRVLPAIAYLEGEYGYSDLYLGVPVILGGNGIEKIIELELLADEKEALDRS
HHHHHHHHHHHHHHHHHEEECCCCCCEEEECCEEEECCCCHHHHHHHHHHCCHHHHHHHH
VESVRNVMKVLV
HHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA