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The map label for this gene is gsiB [H]

Identifier: 49187385

GI number: 49187385

Start: 4302296

End: 4303861

Strand: Reverse

Name: gsiB [H]

Synonym: BAS4390

Alternate gene names: 49187385

Gene position: 4303861-4302296 (Counterclockwise)

Preceding gene: 49187395

Following gene: 49187384

Centisome position: 82.31

GC content: 35.63

Gene sequence:

>1566_bases
ATGAGATTCAAAAGGGGACTTGTGAGTTGTTTTATCGCAATTTTATGTCTTTCAGTTTTTCTAGCGGGGTGTTCATCTAA
TGCGAAAACAGGAAATGAAGGATCAGGAAGCAAAGGGACAAAGGAAGGCGGCGTTTTAACAATTGCTAGACTGTCAGATG
CTGATAATTTAGATCCACATTTTATTACAAACATCCCTTCCGCAAGTGTTGTGTATCATAAAGTGTATGAAAACCTTGTA
CAACGAGATAAAAACATGGATTTTAAGCCGATGTTAGCAAAGGAATGGAAACAAATTGATGATTTAAATTGGGAATTTAA
GTTGCAACAAGGCGTAACATTCCAAGATGGTGCACCATTTAATGCTGAGGCAGTAAAGAAAAACTTTGAACGTGTATTAG
ACCCGAAAGTAGGTTCAAATCGAGCAACTGTTTATTCTATGATTCAAGAAATAAAAGTAATTGATGAATACACAGTACAG
TTCATATTGAAATACCCATACTCACCTCTTTTATCTATTTTCGCAAGTAATGAAGGAAGTATTTTAAGTCCGAAAGCAAT
TGATGAAAAAGGGAAGGGCTTAGCGCAGCATCCAGTTGGGACAGGTCCATATACCTTTAAGTCATGGAAACCAGGTGAAG
AAATTCGACTTGAAAAAAATAAAAATTATTGGGGTGAGAAAGCAAAGGTTGATGAAGTTGTATTCAAAGTAGTACCAGAA
GATGCAACACGTATTGGAATGATCGAAACTAGTGAAGCTCACATTGCAGAGAATTTACCTGTAACTGAAGTAGAGCGAGT
GAAAAATTCACCATCCATGGAGTTAATTGAAAATGAAGGTTTAGGTGTAGAATATATTGGATTTAATGTAGAGAAAAAGC
CATTTGACAATCCGCTCGTTCGTCAAGCGATTGCACATGCAATTGAAACGAAAGGGATTTTAAAAGGGGTATATAACAAT
GTTGGGACTGAAATAAACTCCGTAATGACACCTAAAGTGTTCGGTTATACGAAAGACGTAAAAGGATATAAGTATGACAT
TAATACTGCGAAAAAATTATTAGCAGATGCTGGTTATCCAAATGGATTTAAAACAACAATTTGGACGAATGACAGCAAAG
TTAGAATGGCGTTAGTGGAAGTAATTCAATCTCAATTAAAAGGGATTGGCGTTGATGTAGAAATTAAAGTTATGGAATAT
GGTGCGTTTTTAGCTGCAACGAATAAATCAGAACATGCGATGTTTGTTGGAGGATGGGGAAACGCAACAGGTGATGGTGA
CTATAATCAATATAATTTATTCCATTCTAGTTCACACGGAGCAACTGGTAACCAATTTTTCTATAGTAATCCGGAAGTAG
ATAAATTAATAGAAGAAGCTAGAAAAGAAAAAGATGAGACGAAGAGAAAAGAATTATACAAACAGTTACAAGAGATTGAA
TTAAAAGATGCTTTACTAGTACCAATTCGAGGGATTAATCATATTGCGGCAACTACGAAAAATATAAAAGGATTTTGGAT
TGATCCATCTGGGTATTTGCGACTGGAAGGTGTAGAGCTACAGTAA

Upstream 100 bases:

>100_bases
TGGTGAAGTGATCTGCTATTAGAAAGTAGGGGGATAGAAAGCTAGACTTAAAGTTTCACTTTAGAATGTAGAGATAAAAG
AAAGTGGAGGGGGAAGGGCG

Downstream 100 bases:

>100_bases
AATAGAAAATTCTATCAGTGTAAAAATGATTTTACACTGATAGAAATTTTTATCTGTTTTTGTTTCGTTTGTAAGTAGAA
AAATCATATTGCGAAGGGAG

Product: solute-binding family 5 protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 521; Mature: 521

Protein sequence:

>521_residues
MRFKRGLVSCFIAILCLSVFLAGCSSNAKTGNEGSGSKGTKEGGVLTIARLSDADNLDPHFITNIPSASVVYHKVYENLV
QRDKNMDFKPMLAKEWKQIDDLNWEFKLQQGVTFQDGAPFNAEAVKKNFERVLDPKVGSNRATVYSMIQEIKVIDEYTVQ
FILKYPYSPLLSIFASNEGSILSPKAIDEKGKGLAQHPVGTGPYTFKSWKPGEEIRLEKNKNYWGEKAKVDEVVFKVVPE
DATRIGMIETSEAHIAENLPVTEVERVKNSPSMELIENEGLGVEYIGFNVEKKPFDNPLVRQAIAHAIETKGILKGVYNN
VGTEINSVMTPKVFGYTKDVKGYKYDINTAKKLLADAGYPNGFKTTIWTNDSKVRMALVEVIQSQLKGIGVDVEIKVMEY
GAFLAATNKSEHAMFVGGWGNATGDGDYNQYNLFHSSSHGATGNQFFYSNPEVDKLIEEARKEKDETKRKELYKQLQEIE
LKDALLVPIRGINHIAATTKNIKGFWIDPSGYLRLEGVELQ

Sequences:

>Translated_521_residues
MRFKRGLVSCFIAILCLSVFLAGCSSNAKTGNEGSGSKGTKEGGVLTIARLSDADNLDPHFITNIPSASVVYHKVYENLV
QRDKNMDFKPMLAKEWKQIDDLNWEFKLQQGVTFQDGAPFNAEAVKKNFERVLDPKVGSNRATVYSMIQEIKVIDEYTVQ
FILKYPYSPLLSIFASNEGSILSPKAIDEKGKGLAQHPVGTGPYTFKSWKPGEEIRLEKNKNYWGEKAKVDEVVFKVVPE
DATRIGMIETSEAHIAENLPVTEVERVKNSPSMELIENEGLGVEYIGFNVEKKPFDNPLVRQAIAHAIETKGILKGVYNN
VGTEINSVMTPKVFGYTKDVKGYKYDINTAKKLLADAGYPNGFKTTIWTNDSKVRMALVEVIQSQLKGIGVDVEIKVMEY
GAFLAATNKSEHAMFVGGWGNATGDGDYNQYNLFHSSSHGATGNQFFYSNPEVDKLIEEARKEKDETKRKELYKQLQEIE
LKDALLVPIRGINHIAATTKNIKGFWIDPSGYLRLEGVELQ
>Mature_521_residues
MRFKRGLVSCFIAILCLSVFLAGCSSNAKTGNEGSGSKGTKEGGVLTIARLSDADNLDPHFITNIPSASVVYHKVYENLV
QRDKNMDFKPMLAKEWKQIDDLNWEFKLQQGVTFQDGAPFNAEAVKKNFERVLDPKVGSNRATVYSMIQEIKVIDEYTVQ
FILKYPYSPLLSIFASNEGSILSPKAIDEKGKGLAQHPVGTGPYTFKSWKPGEEIRLEKNKNYWGEKAKVDEVVFKVVPE
DATRIGMIETSEAHIAENLPVTEVERVKNSPSMELIENEGLGVEYIGFNVEKKPFDNPLVRQAIAHAIETKGILKGVYNN
VGTEINSVMTPKVFGYTKDVKGYKYDINTAKKLLADAGYPNGFKTTIWTNDSKVRMALVEVIQSQLKGIGVDVEIKVMEY
GAFLAATNKSEHAMFVGGWGNATGDGDYNQYNLFHSSSHGATGNQFFYSNPEVDKLIEEARKEKDETKRKELYKQLQEIE
LKDALLVPIRGINHIAATTKNIKGFWIDPSGYLRLEGVELQ

Specific function: Part of the ABC transporter complex gsiABCD involved in glutathione import [H]

COG id: COG0747

COG function: function code E; ABC-type dipeptide transport system, periplasmic component

Gene ontology:

Cell location: Periplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 5 family [H]

Homologues:

Organism=Escherichia coli, GI1787052, Length=527, Percent_Identity=31.6888045540797, Blast_Score=279, Evalue=3e-76,
Organism=Escherichia coli, GI1789966, Length=549, Percent_Identity=28.5974499089253, Blast_Score=226, Evalue=2e-60,
Organism=Escherichia coli, GI1787762, Length=476, Percent_Identity=29.6218487394958, Blast_Score=211, Evalue=9e-56,
Organism=Escherichia coli, GI1789887, Length=469, Percent_Identity=26.6524520255864, Blast_Score=152, Evalue=6e-38,
Organism=Escherichia coli, GI1787551, Length=476, Percent_Identity=24.1596638655462, Blast_Score=147, Evalue=2e-36,
Organism=Escherichia coli, GI1787495, Length=522, Percent_Identity=25.095785440613, Blast_Score=137, Evalue=2e-33,
Organism=Escherichia coli, GI87081878, Length=466, Percent_Identity=24.2489270386266, Blast_Score=110, Evalue=2e-25,
Organism=Escherichia coli, GI1789397, Length=469, Percent_Identity=24.0938166311301, Blast_Score=106, Evalue=4e-24,
Organism=Escherichia coli, GI87082063, Length=312, Percent_Identity=24.0384615384615, Blast_Score=73, Evalue=4e-14,
Organism=Escherichia coli, GI1786256, Length=177, Percent_Identity=25.4237288135593, Blast_Score=66, Evalue=7e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000914 [H]

Pfam domain/function: PF00496 SBP_bac_5 [H]

EC number: NA

Molecular weight: Translated: 58168; Mature: 58168

Theoretical pI: Translated: 6.81; Mature: 6.81

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRFKRGLVSCFIAILCLSVFLAGCSSNAKTGNEGSGSKGTKEGGVLTIARLSDADNLDPH
CCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCCC
FITNIPSASVVYHKVYENLVQRDKNMDFKPMLAKEWKQIDDLNWEFKLQQGVTFQDGAPF
EEECCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCEEEECCCCEECCCCCC
NAEAVKKNFERVLDPKVGSNRATVYSMIQEIKVIDEYTVQFILKYPYSPLLSIFASNEGS
CHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCC
ILSPKAIDEKGKGLAQHPVGTGPYTFKSWKPGEEIRLEKNKNYWGEKAKVDEVVFKVVPE
EECCHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEECCCCCCCCCHHHHHHHHHHCCC
DATRIGMIETSEAHIAENLPVTEVERVKNSPSMELIENEGLGVEYIGFNVEKKPFDNPLV
CCCEECEEECCCHHHHCCCCHHHHHHHCCCCCCEEEECCCCCEEEEECCCCCCCCCCHHH
RQAIAHAIETKGILKGVYNNVGTEINSVMTPKVFGYTKDVKGYKYDINTAKKLLADAGYP
HHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCHHHCCCCCCCCEEEEHHHHHHHHHHCCCC
NGFKTTIWTNDSKVRMALVEVIQSQLKGIGVDVEIKVMEYGAFLAATNKSEHAMFVGGWG
CCCEEEEEECCCHHHHHHHHHHHHHHHCCCCCEEEEEEECCEEEEECCCCCCEEEEECCC
NATGDGDYNQYNLFHSSSHGATGNQFFYSNPEVDKLIEEARKEKDETKRKELYKQLQEIE
CCCCCCCCCEEEEEECCCCCCCCCCEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LKDALLVPIRGINHIAATTKNIKGFWIDPSGYLRLEGVELQ
HCCEEEEECCCCCHHHHCCCCCCEEEECCCCCEEEECEECC
>Mature Secondary Structure
MRFKRGLVSCFIAILCLSVFLAGCSSNAKTGNEGSGSKGTKEGGVLTIARLSDADNLDPH
CCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCCC
FITNIPSASVVYHKVYENLVQRDKNMDFKPMLAKEWKQIDDLNWEFKLQQGVTFQDGAPF
EEECCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCEEEECCCCEECCCCCC
NAEAVKKNFERVLDPKVGSNRATVYSMIQEIKVIDEYTVQFILKYPYSPLLSIFASNEGS
CHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCC
ILSPKAIDEKGKGLAQHPVGTGPYTFKSWKPGEEIRLEKNKNYWGEKAKVDEVVFKVVPE
EECCHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEECCCCCCCCCHHHHHHHHHHCCC
DATRIGMIETSEAHIAENLPVTEVERVKNSPSMELIENEGLGVEYIGFNVEKKPFDNPLV
CCCEECEEECCCHHHHCCCCHHHHHHHCCCCCCEEEECCCCCEEEEECCCCCCCCCCHHH
RQAIAHAIETKGILKGVYNNVGTEINSVMTPKVFGYTKDVKGYKYDINTAKKLLADAGYP
HHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCHHHCCCCCCCCEEEEHHHHHHHHHHCCCC
NGFKTTIWTNDSKVRMALVEVIQSQLKGIGVDVEIKVMEYGAFLAATNKSEHAMFVGGWG
CCCEEEEEECCCHHHHHHHHHHHHHHHCCCCCEEEEEEECCEEEEECCCCCCEEEEECCC
NATGDGDYNQYNLFHSSSHGATGNQFFYSNPEVDKLIEEARKEKDETKRKELYKQLQEIE
CCCCCCCCCEEEEEECCCCCCCCCCEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LKDALLVPIRGINHIAATTKNIKGFWIDPSGYLRLEGVELQ
HCCEEEEECCCCCHHHHCCCCCCEEEECCCCCEEEECEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA