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The map label for this gene is tetB [H]

Identifier: 49187375

GI number: 49187375

Start: 4292464

End: 4293789

Strand: Reverse

Name: tetB [H]

Synonym: BAS4380

Alternate gene names: 49187375

Gene position: 4293789-4292464 (Counterclockwise)

Preceding gene: 49187377

Following gene: 49187374

Centisome position: 82.12

GC content: 34.16

Gene sequence:

>1326_bases
ATGGGGAAAGATATAAGTAACCATTCAAAATGGTTTGTTTTCACATTATGTTTTATCGTTTTATTAGGACCGGTGAATGC
GGTTTTATTTAATGTTGCGTTAGAGGATATGGCTCATGATTTATCCATTAGTCAATCGAAAGTAAGTTGGGTTGTAGTAG
GTTACTCCTTAGTTGTCGGTATTGGTTCGATGATATATGGGAAACTGGCTGATCGTTATAGTGTGAAAAAACTATTAATT
ATTTCAATCATCATATTTGTAGCGGGTTCTATTATTGGATTTGTAAATCAATCATATGCGATTGTCATTTTTGCAAGATT
AGTGCAGGCGAGTGGGGGCGCGGCGTTTATTGCGCTTAGTATGATTGCGGTAGCAAAATTAGTTGCTCCTGCTAAGAAGC
CTGGTGCTTTAGCGATGATTAGTTCTTCTATTGCGTTAGCGGTTGGTATTGGTCCTTTAGTTGGTGGGGCTATTACAAAT
ACACTAGGGTGGCCATATTTATTTTTATTTATGATTATCTCAGTATTGGGGATTTTCTTGCTTATAAAATTTATGCCAGG
AGAAGCGCATCATACGGATGAAGTGTTTTATTTTGATTACATTGGAGCGGCGTTACTATTTGTATTTATTACGACTGTTT
TGGTAGGTGTTAATATGAATAGTTGGCTATTTGTGTTATCGATAATTTCCTTATTTTTATTCACGGTTCGTATGAAGAAA
GCGGAGCACCCATTTATCGATATTGAGTTATTTTCGAACAAAGCATTTCTTCGTTTAATAACAGTCGGATTTATAATTAA
TGTGGCGTTATGTGCTAATTTATTATTATTGCCATTACTGTTAGGAAGAGTACACGGATTGTCGCCGTTTATTATCGGAA
TTGTATTATTTGTTGCATCACTTTTCGGTATTGTGTCTAGTTTTATTACTGGAAAGATTATCCCTTCGTTTGGAAATGTG
AATATGATTTATGTAGCGTCTGTCATTATGATTGTTGGCTTTTTAATTTTGGGGTTTATTCCGAATGGAAGTATAGTCGT
TATTGTATTGGCGATTATTTTAACGTTTATGAGTTATTCTGCCATTCAAGTATCATTGAACACATTTATACCGAAAACAT
TACATGTAGCTAAAGTTGGAGTCGGTCTTGGTTTATATAATTTAATTAACTTTTTCGGTATGGCATTTGGACCAGCTGTA
GCAAGCCGAATTATGGAATCTACAAATAGTTATCGTTTTAATTTTATTTTAATCGTCATGTTAATTTCTGCTCATTTCTT
CTTATTAATAGGAATGTCTTCTTTCCGAAAAAAGATGGAGCATTAA

Upstream 100 bases:

>100_bases
CGATAAATATATACATTTGATGAAGTTGTTTAGCAGCGATAGAAGCGCTAAACAACTTTATTTTCATTTACAGTTACTAC
AATCAGGAGAGGTGTTACAC

Downstream 100 bases:

>100_bases
ATATGTACTTTATATAGAAGAAACCGACTTTCCTATAGAAGGTCGGTTATTTTTCTGTCTTGCAAATTATGAAAAAAAGG
AGCAAAATAAAAAAGGTCCT

Product: major facilitator family transporter

Products: Proton [Cytoplasm]; drug [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 441; Mature: 440

Protein sequence:

>441_residues
MGKDISNHSKWFVFTLCFIVLLGPVNAVLFNVALEDMAHDLSISQSKVSWVVVGYSLVVGIGSMIYGKLADRYSVKKLLI
ISIIIFVAGSIIGFVNQSYAIVIFARLVQASGGAAFIALSMIAVAKLVAPAKKPGALAMISSSIALAVGIGPLVGGAITN
TLGWPYLFLFMIISVLGIFLLIKFMPGEAHHTDEVFYFDYIGAALLFVFITTVLVGVNMNSWLFVLSIISLFLFTVRMKK
AEHPFIDIELFSNKAFLRLITVGFIINVALCANLLLLPLLLGRVHGLSPFIIGIVLFVASLFGIVSSFITGKIIPSFGNV
NMIYVASVIMIVGFLILGFIPNGSIVVIVLAIILTFMSYSAIQVSLNTFIPKTLHVAKVGVGLGLYNLINFFGMAFGPAV
ASRIMESTNSYRFNFILIVMLISAHFFLLIGMSSFRKKMEH

Sequences:

>Translated_441_residues
MGKDISNHSKWFVFTLCFIVLLGPVNAVLFNVALEDMAHDLSISQSKVSWVVVGYSLVVGIGSMIYGKLADRYSVKKLLI
ISIIIFVAGSIIGFVNQSYAIVIFARLVQASGGAAFIALSMIAVAKLVAPAKKPGALAMISSSIALAVGIGPLVGGAITN
TLGWPYLFLFMIISVLGIFLLIKFMPGEAHHTDEVFYFDYIGAALLFVFITTVLVGVNMNSWLFVLSIISLFLFTVRMKK
AEHPFIDIELFSNKAFLRLITVGFIINVALCANLLLLPLLLGRVHGLSPFIIGIVLFVASLFGIVSSFITGKIIPSFGNV
NMIYVASVIMIVGFLILGFIPNGSIVVIVLAIILTFMSYSAIQVSLNTFIPKTLHVAKVGVGLGLYNLINFFGMAFGPAV
ASRIMESTNSYRFNFILIVMLISAHFFLLIGMSSFRKKMEH
>Mature_440_residues
GKDISNHSKWFVFTLCFIVLLGPVNAVLFNVALEDMAHDLSISQSKVSWVVVGYSLVVGIGSMIYGKLADRYSVKKLLII
SIIIFVAGSIIGFVNQSYAIVIFARLVQASGGAAFIALSMIAVAKLVAPAKKPGALAMISSSIALAVGIGPLVGGAITNT
LGWPYLFLFMIISVLGIFLLIKFMPGEAHHTDEVFYFDYIGAALLFVFITTVLVGVNMNSWLFVLSIISLFLFTVRMKKA
EHPFIDIELFSNKAFLRLITVGFIINVALCANLLLLPLLLGRVHGLSPFIIGIVLFVASLFGIVSSFITGKIIPSFGNVN
MIYVASVIMIVGFLILGFIPNGSIVVIVLAIILTFMSYSAIQVSLNTFIPKTLHVAKVGVGLGLYNLINFFGMAFGPAVA
SRIMESTNSYRFNFILIVMLISAHFFLLIGMSSFRKKMEH

Specific function: Resistance to tetracycline by an active tetracycline efflux. This is an energy-dependent process that decreases the accumulation of the antibiotic in whole cells. This protein functions as a metal-tetracycline/H(+) antiporter [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. TCR/tet family [H]

Homologues:

Organism=Escherichia coli, GI1790195, Length=440, Percent_Identity=22.7272727272727, Blast_Score=88, Evalue=1e-18,
Organism=Escherichia coli, GI1788710, Length=280, Percent_Identity=25, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1789042, Length=308, Percent_Identity=25, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI87081983, Length=305, Percent_Identity=24.9180327868852, Blast_Score=70, Evalue=4e-13,
Organism=Escherichia coli, GI1788392, Length=207, Percent_Identity=25.1207729468599, Blast_Score=62, Evalue=8e-11,
Organism=Saccharomyces cerevisiae, GI6322958, Length=123, Percent_Identity=34.9593495934959, Blast_Score=80, Evalue=9e-16,
Organism=Saccharomyces cerevisiae, GI6325455, Length=171, Percent_Identity=26.9005847953216, Blast_Score=72, Evalue=2e-13,
Organism=Saccharomyces cerevisiae, GI6321663, Length=135, Percent_Identity=29.6296296296296, Blast_Score=71, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR001411 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 47894; Mature: 47763

Theoretical pI: Translated: 10.14; Mature: 10.14

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
GKDISNHSKWFVFTLCFIVLLGPVNAVLFNVALEDMAHDLSISQSKVSWVVVGYSLVVG
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHEEHHHHHHHH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HHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; drug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + drug [Cytoplasm] = Proton [Cytoplasm] + drug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2844262; 7584024; 9384377 [H]