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The map label for this gene is folC [H]

Identifier: 49187349

GI number: 49187349

Start: 4262612

End: 4263913

Strand: Reverse

Name: folC [H]

Synonym: BAS4354

Alternate gene names: 49187349

Gene position: 4263913-4262612 (Counterclockwise)

Preceding gene: 49187350

Following gene: 49187348

Centisome position: 81.55

GC content: 37.02

Gene sequence:

>1302_bases
GTGATACATACATACGAAGAAGCGATAGGGTGGATTCATAGCCGATTGAAATTTGGTATTAAACCGGGATTAGAAAGAAT
GAGATGGATGCTAGAAGAGCTCGGAAATCCAGAGCGCCACATAAAATGTGTTCATCTTGCTGGTACAAATGGAAAAGGTT
CAACGTTAACATATATGCGCTACATGTTAGAAGACGCAAAATATAAGGTAGGAACATTTACCTCACCTTATATTGAAACA
TTTAACGAGCGTATTAGTGTGAACGGAACACCAATTGCAGATGAAGAGATTACTGAACTTGTAAAGATGGTAAAGCCAGT
CGTTGAAAAACTAGACGAGACGGATTTAGGGGAAGCGACTGAGTTTGAAATTATTACTGTTATGGCAATTTGTTATTTCG
GTAAAGTTAATTTCTGTGATGTTGTTTTATTTGAAACAGGACTTGGAGGGCGTTTTGACTCTACAAATGTGATTCATCCT
GTTCTCACGATTATTACGAATATCGGCCACGATCATATGCACATTTTAGGTAATACACTAGGAGAAATTGCATATGAAAA
GGCGGGGATTATTAAGTCTGGTGTTCCGGTTATTACTGGTGTGCAAGATGAGGAAGCGTTGCAAGTCATTCAAAAAGTTG
CAAAGGAAAATCGTGCAAACTTATATGAGATGGGCAACCATTTTACAGCGTTACATAAACAGTCTAGTGAAAATGGCGAA
CAGTTTGATTTCACTTGTCCTTTCGCCTCTTTTGAAGATGTGCGGATCGCAATGAAAGGCAGCCATCAAGTAGGAAACGC
AGCACTTGCACTTATGGCAGTCATGTATGTAAAAACATACTTATCATTTTTAATTGAGGAAGAAGAAATAAGAACTGGAT
TACAGGAAGCGTATTGGGTAGGGCGCTTTGAAAAATTGCAAAGTAATCCAGATATTATTATAGATGGTGCTCATAACCCA
GAAGGTATTGAAAGCCTTGTGAAAACGGTAGAATCACATTACAAAGATAAAAACGTAATAGTTTTATTTACTGCTCTTGG
TGATAAACAATTGCATAATATGGTAGGTCAATTAGAAACCATTGCCGATGAAATTATTTTTACAACATTCGCCTTTGATC
GTGCTATTTCAGCAGGCGAGCTTGCGTCGTATGCAAAGAAAGAGTCAAAATTAGTTTTTGAAAATTGGAAAGAGGCAATT
GATACGAAGGTTGAAATGATTGGAGAAAATGATGTTTTTATCATAACAGGTTCTCTTTATTTCATTTCGGAAGTTCGAAA
ATATATTCGCGAGAAAAACTAG

Upstream 100 bases:

>100_bases
GAAATAAAAATGTTTTTTAGAGACTCACTATATTGGTGAGTCTCTTTTCTTTGCGTATAATAGAAAGATGAAGGTTGTCT
TTTGAAAGGGGAAAGCATAC

Downstream 100 bases:

>100_bases
ATAGTCTTTGCTATCTAGTTTTTTTGTTCTACAAATGAAGTCAAATGCATACATATAAGATAAATATATAGGGTTATTTG
GCGAATTTCTCTGACAAATA

Product: folylpolyglutamate synthase

Products: NA

Alternate protein names: Folylpoly-gamma-glutamate synthetase; FPGS; Tetrahydrofolate synthase; Tetrahydrofolylpolyglutamate synthase [H]

Number of amino acids: Translated: 433; Mature: 433

Protein sequence:

>433_residues
MIHTYEEAIGWIHSRLKFGIKPGLERMRWMLEELGNPERHIKCVHLAGTNGKGSTLTYMRYMLEDAKYKVGTFTSPYIET
FNERISVNGTPIADEEITELVKMVKPVVEKLDETDLGEATEFEIITVMAICYFGKVNFCDVVLFETGLGGRFDSTNVIHP
VLTIITNIGHDHMHILGNTLGEIAYEKAGIIKSGVPVITGVQDEEALQVIQKVAKENRANLYEMGNHFTALHKQSSENGE
QFDFTCPFASFEDVRIAMKGSHQVGNAALALMAVMYVKTYLSFLIEEEEIRTGLQEAYWVGRFEKLQSNPDIIIDGAHNP
EGIESLVKTVESHYKDKNVIVLFTALGDKQLHNMVGQLETIADEIIFTTFAFDRAISAGELASYAKKESKLVFENWKEAI
DTKVEMIGENDVFIITGSLYFISEVRKYIREKN

Sequences:

>Translated_433_residues
MIHTYEEAIGWIHSRLKFGIKPGLERMRWMLEELGNPERHIKCVHLAGTNGKGSTLTYMRYMLEDAKYKVGTFTSPYIET
FNERISVNGTPIADEEITELVKMVKPVVEKLDETDLGEATEFEIITVMAICYFGKVNFCDVVLFETGLGGRFDSTNVIHP
VLTIITNIGHDHMHILGNTLGEIAYEKAGIIKSGVPVITGVQDEEALQVIQKVAKENRANLYEMGNHFTALHKQSSENGE
QFDFTCPFASFEDVRIAMKGSHQVGNAALALMAVMYVKTYLSFLIEEEEIRTGLQEAYWVGRFEKLQSNPDIIIDGAHNP
EGIESLVKTVESHYKDKNVIVLFTALGDKQLHNMVGQLETIADEIIFTTFAFDRAISAGELASYAKKESKLVFENWKEAI
DTKVEMIGENDVFIITGSLYFISEVRKYIREKN
>Mature_433_residues
MIHTYEEAIGWIHSRLKFGIKPGLERMRWMLEELGNPERHIKCVHLAGTNGKGSTLTYMRYMLEDAKYKVGTFTSPYIET
FNERISVNGTPIADEEITELVKMVKPVVEKLDETDLGEATEFEIITVMAICYFGKVNFCDVVLFETGLGGRFDSTNVIHP
VLTIITNIGHDHMHILGNTLGEIAYEKAGIIKSGVPVITGVQDEEALQVIQKVAKENRANLYEMGNHFTALHKQSSENGE
QFDFTCPFASFEDVRIAMKGSHQVGNAALALMAVMYVKTYLSFLIEEEEIRTGLQEAYWVGRFEKLQSNPDIIIDGAHNP
EGIESLVKTVESHYKDKNVIVLFTALGDKQLHNMVGQLETIADEIIFTTFAFDRAISAGELASYAKKESKLVFENWKEAI
DTKVEMIGENDVFIITGSLYFISEVRKYIREKN

Specific function: Conversion of folates to polyglutamate derivatives. It preferes 5,10-methylenetetrahydrofolate, rather than 10- formyltetrahydrofolate as folate substrate [H]

COG id: COG0285

COG function: function code H; Folylpolyglutamate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the folylpolyglutamate synthase family [H]

Homologues:

Organism=Homo sapiens, GI66932984, Length=342, Percent_Identity=28.0701754385965, Blast_Score=136, Evalue=3e-32,
Organism=Homo sapiens, GI66932990, Length=342, Percent_Identity=28.0701754385965, Blast_Score=136, Evalue=4e-32,
Organism=Escherichia coli, GI1788654, Length=427, Percent_Identity=27.8688524590164, Blast_Score=142, Evalue=6e-35,
Organism=Caenorhabditis elegans, GI17553148, Length=412, Percent_Identity=25.2427184466019, Blast_Score=135, Evalue=3e-32,
Organism=Caenorhabditis elegans, GI17553150, Length=412, Percent_Identity=25.2427184466019, Blast_Score=135, Evalue=3e-32,
Organism=Caenorhabditis elegans, GI71984923, Length=412, Percent_Identity=25.2427184466019, Blast_Score=135, Evalue=3e-32,
Organism=Saccharomyces cerevisiae, GI6323760, Length=434, Percent_Identity=29.4930875576037, Blast_Score=184, Evalue=2e-47,
Organism=Saccharomyces cerevisiae, GI6324815, Length=364, Percent_Identity=28.2967032967033, Blast_Score=134, Evalue=3e-32,
Organism=Saccharomyces cerevisiae, GI6322718, Length=292, Percent_Identity=22.9452054794521, Blast_Score=69, Evalue=1e-12,
Organism=Drosophila melanogaster, GI24641571, Length=335, Percent_Identity=29.8507462686567, Blast_Score=132, Evalue=4e-31,
Organism=Drosophila melanogaster, GI24581568, Length=204, Percent_Identity=30.8823529411765, Blast_Score=90, Evalue=3e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018109
- InterPro:   IPR001645
- InterPro:   IPR004101
- InterPro:   IPR013221 [H]

Pfam domain/function: PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.17 [H]

Molecular weight: Translated: 48847; Mature: 48847

Theoretical pI: Translated: 4.94; Mature: 4.94

Prosite motif: PS01011 FOLYLPOLYGLU_SYNT_1 ; PS01012 FOLYLPOLYGLU_SYNT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIHTYEEAIGWIHSRLKFGIKPGLERMRWMLEELGNPERHIKCVHLAGTNGKGSTLTYMR
CCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCEEEEEEEECCCCCCCHHHHHH
YMLEDAKYKVGTFTSPYIETFNERISVNGTPIADEEITELVKMVKPVVEKLDETDLGEAT
HHHHHCCEECCCCCCHHHHHHHCEEECCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCC
EFEIITVMAICYFGKVNFCDVVLFETGLGGRFDSTNVIHPVLTIITNIGHDHMHILGNTL
HHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHH
GEIAYEKAGIIKSGVPVITGVQDEEALQVIQKVAKENRANLYEMGNHFTALHKQSSENGE
HHHHHHHCCCCCCCCCEEECCCCHHHHHHHHHHHHHCCCCHHHHCCHHHHHHCCCCCCCC
QFDFTCPFASFEDVRIAMKGSHQVGNAALALMAVMYVKTYLSFLIEEEEIRTGLQEAYWV
EEEEECCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GRFEKLQSNPDIIIDGAHNPEGIESLVKTVESHYKDKNVIVLFTALGDKQLHNMVGQLET
HHHHHHCCCCCEEEECCCCHHHHHHHHHHHHHHCCCCCEEEEEEECCCHHHHHHHHHHHH
IADEIIFTTFAFDRAISAGELASYAKKESKLVFENWKEAIDTKVEMIGENDVFIITGSLY
HHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECHHH
FISEVRKYIREKN
HHHHHHHHHHCCC
>Mature Secondary Structure
MIHTYEEAIGWIHSRLKFGIKPGLERMRWMLEELGNPERHIKCVHLAGTNGKGSTLTYMR
CCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCEEEEEEEECCCCCCCHHHHHH
YMLEDAKYKVGTFTSPYIETFNERISVNGTPIADEEITELVKMVKPVVEKLDETDLGEAT
HHHHHCCEECCCCCCHHHHHHHCEEECCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCC
EFEIITVMAICYFGKVNFCDVVLFETGLGGRFDSTNVIHPVLTIITNIGHDHMHILGNTL
HHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHH
GEIAYEKAGIIKSGVPVITGVQDEEALQVIQKVAKENRANLYEMGNHFTALHKQSSENGE
HHHHHHHCCCCCCCCCEEECCCCHHHHHHHHHHHHHCCCCHHHHCCHHHHHHCCCCCCCC
QFDFTCPFASFEDVRIAMKGSHQVGNAALALMAVMYVKTYLSFLIEEEEIRTGLQEAYWV
EEEEECCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GRFEKLQSNPDIIIDGAHNPEGIESLVKTVESHYKDKNVIVLFTALGDKQLHNMVGQLET
HHHHHHCCCCCEEEECCCCHHHHHHHHHHHHHHCCCCCEEEEEEECCCHHHHHHHHHHHH
IADEIIFTTFAFDRAISAGELASYAKKESKLVFENWKEAIDTKVEMIGENDVFIITGSLY
HHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECHHH
FISEVRKYIREKN
HHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8419299; 9384377; 2553669 [H]