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The map label for this gene is yfiY [H]

Identifier: 49187312

GI number: 49187312

Start: 4229030

End: 4229974

Strand: Direct

Name: yfiY [H]

Synonym: BAS4317

Alternate gene names: 49187312

Gene position: 4229030-4229974 (Clockwise)

Preceding gene: 49187305

Following gene: 49187317

Centisome position: 80.88

GC content: 34.71

Gene sequence:

>945_bases
ATGAAACGTAAGCTATTTATTTTATTTACTATTATGCTAGTTGTTCTTTCTATCGTTGGCTGTTCTTCTCAAAAAGAAGA
ATCAAAAGCAAAAGAACAACCGAAAACAAAAGTTGTAAAACACGCTAAAGGGGAAGCTACAATTCCAGTAAATCCAAAGA
GAATTGTTGACTTATCTGGATCAACAGAAGAATTATTACTTCTTGGACACAAACCTGTTGGTACAGCAAATACATACAAA
GATAAAATCCAAAAGCATTTGACAGAAAAGCTAGATGGAGTAAAGGCAGTAGGTTGGTACTGGGCACCTAAAGTTGATTT
AGAAGCTGTTACTGCTTTGAAACCAGACTTAATTATTTTGAACAATCGTCAATTAAAAATTTATGATCAACTAGAAAAGG
TTGCACCGACAGTTGTTCTAGAAACAAACTTAGAAGACTGGCGCGGTAAGTTTAAAGAAGTAGGTAAACTATTTGACGAA
GAGAAAAAGGCAGACAAATGGATTGCAGACTACGATAAAAAAGCCGACTCTTTATCTAAAAAGATTAAAGAGAAAACAAA
AGATGACAGCTTTATGTTCGTCGCAGTTACACCACAAAACTTCCGTGTATACGGTAGCTTCGGGTACGGTGACATCATCT
TTAACGACTTAAAACTTCCAGCAACAAAAGGTACAGATTTAAAACAAACGATGGCGCAAGTATCGCTAGAAGGTCTTGTT
GCATTCCAACCTGACCAAATGTTTATTGTAAACTTTGGCGGCGAAGCTGATAAAGTTTACGAAGACTACAAAAATAGTGC
TGTTTGGAAAGACAATAAAGCAGTAAAAAATAATCACGTATATGAAGTATCGAATGAAGTCTTCAATACGAAAGCTTTCA
ATCCAATCGGAAAAGATATGCTAATTGATGAAATCGCAAAAGAAATTTTAGCTAAGAATAAATAA

Upstream 100 bases:

>100_bases
TTAAAACTTCTGTTATTTTCCATTGACAGAAAAGTTAAAGCACATTAATATACAGAAGTCGATAATGATTATCAATACTA
ATTAAAGGGGTTACATCAGG

Downstream 100 bases:

>100_bases
GAAAAAAGCAGCTCACCATGAGCTGCTTTTTTTCTTATTTTGCACTGCGTATTGTTTCATTTTTTCAATCGTCTTCACAA
AACCTTCTTTTGATTTAATC

Product: substrate-binding family protein

Products: ADP; phosphate; ferric enterobactin [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 314; Mature: 314

Protein sequence:

>314_residues
MKRKLFILFTIMLVVLSIVGCSSQKEESKAKEQPKTKVVKHAKGEATIPVNPKRIVDLSGSTEELLLLGHKPVGTANTYK
DKIQKHLTEKLDGVKAVGWYWAPKVDLEAVTALKPDLIILNNRQLKIYDQLEKVAPTVVLETNLEDWRGKFKEVGKLFDE
EKKADKWIADYDKKADSLSKKIKEKTKDDSFMFVAVTPQNFRVYGSFGYGDIIFNDLKLPATKGTDLKQTMAQVSLEGLV
AFQPDQMFIVNFGGEADKVYEDYKNSAVWKDNKAVKNNHVYEVSNEVFNTKAFNPIGKDMLIDEIAKEILAKNK

Sequences:

>Translated_314_residues
MKRKLFILFTIMLVVLSIVGCSSQKEESKAKEQPKTKVVKHAKGEATIPVNPKRIVDLSGSTEELLLLGHKPVGTANTYK
DKIQKHLTEKLDGVKAVGWYWAPKVDLEAVTALKPDLIILNNRQLKIYDQLEKVAPTVVLETNLEDWRGKFKEVGKLFDE
EKKADKWIADYDKKADSLSKKIKEKTKDDSFMFVAVTPQNFRVYGSFGYGDIIFNDLKLPATKGTDLKQTMAQVSLEGLV
AFQPDQMFIVNFGGEADKVYEDYKNSAVWKDNKAVKNNHVYEVSNEVFNTKAFNPIGKDMLIDEIAKEILAKNK
>Mature_314_residues
MKRKLFILFTIMLVVLSIVGCSSQKEESKAKEQPKTKVVKHAKGEATIPVNPKRIVDLSGSTEELLLLGHKPVGTANTYK
DKIQKHLTEKLDGVKAVGWYWAPKVDLEAVTALKPDLIILNNRQLKIYDQLEKVAPTVVLETNLEDWRGKFKEVGKLFDE
EKKADKWIADYDKKADSLSKKIKEKTKDDSFMFVAVTPQNFRVYGSFGYGDIIFNDLKLPATKGTDLKQTMAQVSLEGLV
AFQPDQMFIVNFGGEADKVYEDYKNSAVWKDNKAVKNNHVYEVSNEVFNTKAFNPIGKDMLIDEIAKEILAKNK

Specific function: Part of the ABC transporter complex yfiYZ/yfhA/yusV involved in import of the iron-hydroxamate siderophores schizokinen, arthrobactin and corprogen. Binds the siderophores and delivers them to the surface of yfiZ/yfhA (Probable) [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Probable). Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Fe/B12 periplasmic-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1786807, Length=276, Percent_Identity=23.9130434782609, Blast_Score=64, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002491 [H]

Pfam domain/function: PF01497 Peripla_BP_2 [H]

EC number: NA

Molecular weight: Translated: 35521; Mature: 35521

Theoretical pI: Translated: 9.52; Mature: 9.52

Prosite motif: PS00013 PROKAR_LIPOPROTEIN ; PS50983 FE_B12_PBP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRKLFILFTIMLVVLSIVGCSSQKEESKAKEQPKTKVVKHAKGEATIPVNPKRIVDLSG
CCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCHHHHHHHCCCCEECCCCCCEEEECCC
STEELLLLGHKPVGTANTYKDKIQKHLTEKLDGVKAVGWYWAPKVDLEAVTALKPDLIIL
CCCEEEEEECCCCCCCHHHHHHHHHHHHHHHCCCEEEEEEECCCCCHHHHHHCCCCEEEE
NNRQLKIYDQLEKVAPTVVLETNLEDWRGKFKEVGKLFDEEKKADKWIADYDKKADSLSK
CCCEEEHHHHHHHHCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KIKEKTKDDSFMFVAVTPQNFRVYGSFGYGDIIFNDLKLPATKGTDLKQTMAQVSLEGLV
HHHHHCCCCCEEEEEECCCCEEEEEECCCCCEEEECEECCCCCCCCHHHHHHHHHHHHEE
AFQPDQMFIVNFGGEADKVYEDYKNSAVWKDNKAVKNNHVYEVSNEVFNTKAFNPIGKDM
EECCCCEEEEEECCCHHHHHHHHCCCCEECCCCCCCCCCEEEEHHHHHCCCCCCCCCHHH
LIDEIAKEILAKNK
HHHHHHHHHHHCCC
>Mature Secondary Structure
MKRKLFILFTIMLVVLSIVGCSSQKEESKAKEQPKTKVVKHAKGEATIPVNPKRIVDLSG
CCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCHHHHHHHCCCCEECCCCCCEEEECCC
STEELLLLGHKPVGTANTYKDKIQKHLTEKLDGVKAVGWYWAPKVDLEAVTALKPDLIIL
CCCEEEEEECCCCCCCHHHHHHHHHHHHHHHCCCEEEEEEECCCCCHHHHHHCCCCEEEE
NNRQLKIYDQLEKVAPTVVLETNLEDWRGKFKEVGKLFDEEKKADKWIADYDKKADSLSK
CCCEEEHHHHHHHHCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KIKEKTKDDSFMFVAVTPQNFRVYGSFGYGDIIFNDLKLPATKGTDLKQTMAQVSLEGLV
HHHHHCCCCCEEEEEECCCCEEEEEECCCCCEEEECEECCCCCCCCHHHHHHHHHHHHEE
AFQPDQMFIVNFGGEADKVYEDYKNSAVWKDNKAVKNNHVYEVSNEVFNTKAFNPIGKDM
EECCCCEEEEEECCCHHHHHHHHCCCCEECCCCCCCCCCEEEEHHHHHCCCCCCCCCHHH
LIDEIAKEILAKNK
HHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; ferric enterobactin [Periplasm]; H2O [C]

Specific reaction: ATP + ferric enterobactin [Periplasm] + H2O = ADP + phosphate + ferric enterobactin [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8946165; 9384377 [H]