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The map label for this gene is tgt

Identifier: 49187307

GI number: 49187307

Start: 4224796

End: 4225935

Strand: Reverse

Name: tgt

Synonym: BAS4312

Alternate gene names: 49187307

Gene position: 4225935-4224796 (Counterclockwise)

Preceding gene: 49187308

Following gene: 49187306

Centisome position: 80.82

GC content: 40.09

Gene sequence:

>1140_bases
ATGACAGCAATTCGTTATGAATTTATAAAAACTTGTAAACAAACAGGTGCTCGTTTAGGACGTGTACATACGCCGCACGG
TTCATTTGATACACCAACATTTATGCCAGTTGGTACACTTGCAACAGTTAAGACAATGTCACCAGAAGAATTAAAAGCAA
TGGATTCTGGCATTATTTTAAGTAATACGTATCATTTATGGTTACGTCCAGGTCACGAAATCATTCGTGAAGCAGGTGGT
TTGCATAAATTTATGAACTGGGATCGTGCAATCTTAACGGACTCTGGTGGTTTCCAAGTATTCAGTTTAAGTGACTTCCG
TCGCATTGAAGAGGAAGGCGTTCACTTCCGTAACCACTTAAACGGAGATAAATTATTTTTATCACCAGAAAAAGCGATGG
AAATTCAAAATGCATTAGGTTCAGATATTATGATGGCATTTGATGAGTGTCCACCATTCCCAGCTACTTTTGAATATATG
AAGAAGTCTGTAGAGCGTACAAGTCGCTGGGCAGAGCGTTGCTTAAAAGCGCATGAACGTCCACAAGATCAAGGTTTATT
CGGTATTGTACAGGGCGGAGAATTTGAAGAACTTCGCCGTCAAAGTGCAAAAGACCTTGTTTCAATGGACTTCCCTGGTT
ACGCGGTAGGTGGTTTATCTGTAGGTGAGCCGAAAGATATTATGAACCGTGTACTTGAGTTTACAACGCCACTTCTTCCA
GATAATAAGCCACGTTACTTAATGGGAGTAGGTTCTCCTGATTCATTAATCGATGGTGCAATTCGTGGTATTGATATGTT
TGACTGCGTACTGCCAACTCGTATCGCTCGAAATGGTACATGTATGACAAGTGAAGGCCGCCTTGTTGTTAAAAATGCGA
AATTCGCAAGAGACTTCGGTCCGCTTGATCCAAATTGTGATTGCTACACATGTAAAAATTATTCTCGTGCGTATATTCGT
CACTTAATGAAATGTGATGAAACGTTCGGAATTCGTTTAACGTCTTATCACAACCTTCATTTTCTGTTAAACTTAATGGA
GCAGGTGAGACAAGCTATTCGTGAAGACCGTCTTGGCGATTTCCGCGAAGAATTCTTTGAACAGTATGGCTTTAATAAAC
CGAATGCTAAAAACTTCTAA

Upstream 100 bases:

>100_bases
TAATGAAGCAGTAAAAGAAAAATATCGTTTCTTTAGCTTCGGTGATGCAATGTTCGTTGCATCTCACGCTAAAATGGGAA
ACAAATAAAAGGAGTAAATT

Downstream 100 bases:

>100_bases
TACATAATTTAAAAGGAGGAACAAAAGATGAATGCAGGTATGATGAATATCGTTATGATCGTTGCGATGTTTGCGATTTT
CTATTTCTTATTAATTCGCC

Product: queuine tRNA-ribosyltransferase

Products: NA

Alternate protein names: Guanine insertion enzyme; tRNA-guanine transglycosylase

Number of amino acids: Translated: 379; Mature: 378

Protein sequence:

>379_residues
MTAIRYEFIKTCKQTGARLGRVHTPHGSFDTPTFMPVGTLATVKTMSPEELKAMDSGIILSNTYHLWLRPGHEIIREAGG
LHKFMNWDRAILTDSGGFQVFSLSDFRRIEEEGVHFRNHLNGDKLFLSPEKAMEIQNALGSDIMMAFDECPPFPATFEYM
KKSVERTSRWAERCLKAHERPQDQGLFGIVQGGEFEELRRQSAKDLVSMDFPGYAVGGLSVGEPKDIMNRVLEFTTPLLP
DNKPRYLMGVGSPDSLIDGAIRGIDMFDCVLPTRIARNGTCMTSEGRLVVKNAKFARDFGPLDPNCDCYTCKNYSRAYIR
HLMKCDETFGIRLTSYHNLHFLLNLMEQVRQAIREDRLGDFREEFFEQYGFNKPNAKNF

Sequences:

>Translated_379_residues
MTAIRYEFIKTCKQTGARLGRVHTPHGSFDTPTFMPVGTLATVKTMSPEELKAMDSGIILSNTYHLWLRPGHEIIREAGG
LHKFMNWDRAILTDSGGFQVFSLSDFRRIEEEGVHFRNHLNGDKLFLSPEKAMEIQNALGSDIMMAFDECPPFPATFEYM
KKSVERTSRWAERCLKAHERPQDQGLFGIVQGGEFEELRRQSAKDLVSMDFPGYAVGGLSVGEPKDIMNRVLEFTTPLLP
DNKPRYLMGVGSPDSLIDGAIRGIDMFDCVLPTRIARNGTCMTSEGRLVVKNAKFARDFGPLDPNCDCYTCKNYSRAYIR
HLMKCDETFGIRLTSYHNLHFLLNLMEQVRQAIREDRLGDFREEFFEQYGFNKPNAKNF
>Mature_378_residues
TAIRYEFIKTCKQTGARLGRVHTPHGSFDTPTFMPVGTLATVKTMSPEELKAMDSGIILSNTYHLWLRPGHEIIREAGGL
HKFMNWDRAILTDSGGFQVFSLSDFRRIEEEGVHFRNHLNGDKLFLSPEKAMEIQNALGSDIMMAFDECPPFPATFEYMK
KSVERTSRWAERCLKAHERPQDQGLFGIVQGGEFEELRRQSAKDLVSMDFPGYAVGGLSVGEPKDIMNRVLEFTTPLLPD
NKPRYLMGVGSPDSLIDGAIRGIDMFDCVLPTRIARNGTCMTSEGRLVVKNAKFARDFGPLDPNCDCYTCKNYSRAYIRH
LMKCDETFGIRLTSYHNLHFLLNLMEQVRQAIREDRLGDFREEFFEQYGFNKPNAKNF

Specific function: Exchanges the guanine residue with 7-aminomethyl-7- deazaguanine in tRNAs with GU(N) anticodons (tRNA-Asp, -Asn, -His and -Tyr). After this exchange, a cyclopentendiol moiety is attached to the 7-aminomethyl group of 7-deazaguanine, resulting in the hyper

COG id: COG0343

COG function: function code J; Queuine/archaeosine tRNA-ribosyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the queuine tRNA-ribosyltransferase family

Homologues:

Organism=Homo sapiens, GI13654276, Length=364, Percent_Identity=45.6043956043956, Blast_Score=345, Evalue=6e-95,
Organism=Homo sapiens, GI13375872, Length=292, Percent_Identity=26.7123287671233, Blast_Score=114, Evalue=2e-25,
Organism=Escherichia coli, GI1786607, Length=359, Percent_Identity=55.7103064066852, Blast_Score=420, Evalue=1e-119,
Organism=Caenorhabditis elegans, GI17544680, Length=354, Percent_Identity=48.8700564971751, Blast_Score=334, Evalue=4e-92,
Organism=Caenorhabditis elegans, GI25146502, Length=247, Percent_Identity=28.7449392712551, Blast_Score=85, Evalue=5e-17,
Organism=Drosophila melanogaster, GI19920510, Length=371, Percent_Identity=44.2048517520216, Blast_Score=331, Evalue=6e-91,
Organism=Drosophila melanogaster, GI21355311, Length=263, Percent_Identity=25.0950570342205, Blast_Score=85, Evalue=1e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TGT_BACAA (C3P9A4)

Other databases:

- EMBL:   CP001598
- RefSeq:   YP_002868703.1
- ProteinModelPortal:   C3P9A4
- SMR:   C3P9A4
- EnsemblBacteria:   EBBACT00000128877
- GeneID:   7850728
- GenomeReviews:   CP001598_GR
- KEGG:   bai:BAA_4664
- GeneTree:   EBGT00050000001431
- ProtClustDB:   PRK00112
- HAMAP:   MF_00168
- InterPro:   IPR004803
- InterPro:   IPR002616
- Gene3D:   G3DSA:3.20.20.105
- PANTHER:   PTHR11962
- TIGRFAMs:   TIGR00430
- TIGRFAMs:   TIGR00449

Pfam domain/function: PF01702 TGT; SSF51713 tRNA_ribo_trans

EC number: =2.4.2.29

Molecular weight: Translated: 43228; Mature: 43097

Theoretical pI: Translated: 7.07; Mature: 7.07

Prosite motif: NA

Important sites: ACT_SITE 94-94 BINDING 95-95

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
6.6 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
6.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAIRYEFIKTCKQTGARLGRVHTPHGSFDTPTFMPVGTLATVKTMSPEELKAMDSGIIL
CCCHHHHHHHHHHHHHHHHCCEECCCCCCCCCCCCCCCCHHHEECCCHHHHHHHHCCEEE
SNTYHLWLRPGHEIIREAGGLHKFMNWDRAILTDSGGFQVFSLSDFRRIEEEGVHFRNHL
ECCEEEEECCCHHHHHHHCCHHHHHCCCCEEEECCCCEEEEECHHHHHHHHHCCHHHHCC
NGDKLFLSPEKAMEIQNALGSDIMMAFDECPPFPATFEYMKKSVERTSRWAERCLKAHER
CCCEEEECHHHHHHHHHHHCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCC
PQDQGLFGIVQGGEFEELRRQSAKDLVSMDFPGYAVGGLSVGEPKDIMNRVLEFTTPLLP
CCCCCCEEEECCCCHHHHHHHHHHHHHCCCCCCEEECCCCCCCHHHHHHHHHHHHCCCCC
DNKPRYLMGVGSPDSLIDGAIRGIDMFDCVLPTRIARNGTCMTSEGRLVVKNAKFARDFG
CCCCCEEEECCCCHHHHHHHHHCCHHHHHHHHHHHHCCCCEEECCCEEEEECCHHHHHCC
PLDPNCDCYTCKNYSRAYIRHLMKCDETFGIRLTSYHNLHFLLNLMEQVRQAIREDRLGD
CCCCCCCEEECCCHHHHHHHHHHHCCHHHCEEEECCCHHHHHHHHHHHHHHHHHHCCCCH
FREEFFEQYGFNKPNAKNF
HHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure 
TAIRYEFIKTCKQTGARLGRVHTPHGSFDTPTFMPVGTLATVKTMSPEELKAMDSGIIL
CCHHHHHHHHHHHHHHHHCCEECCCCCCCCCCCCCCCCHHHEECCCHHHHHHHHCCEEE
SNTYHLWLRPGHEIIREAGGLHKFMNWDRAILTDSGGFQVFSLSDFRRIEEEGVHFRNHL
ECCEEEEECCCHHHHHHHCCHHHHHCCCCEEEECCCCEEEEECHHHHHHHHHCCHHHHCC
NGDKLFLSPEKAMEIQNALGSDIMMAFDECPPFPATFEYMKKSVERTSRWAERCLKAHER
CCCEEEECHHHHHHHHHHHCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCC
PQDQGLFGIVQGGEFEELRRQSAKDLVSMDFPGYAVGGLSVGEPKDIMNRVLEFTTPLLP
CCCCCCEEEECCCCHHHHHHHHHHHHHCCCCCCEEECCCCCCCHHHHHHHHHHHHCCCCC
DNKPRYLMGVGSPDSLIDGAIRGIDMFDCVLPTRIARNGTCMTSEGRLVVKNAKFARDFG
CCCCCEEEECCCCHHHHHHHHHCCHHHHHHHHHHHHCCCCEEECCCEEEEECCHHHHHCC
PLDPNCDCYTCKNYSRAYIRHLMKCDETFGIRLTSYHNLHFLLNLMEQVRQAIREDRLGD
CCCCCCCEEECCCHHHHHHHHHHHCCHHHCEEEECCCHHHHHHHHHHHHHHHHHHCCCCH
FREEFFEQYGFNKPNAKNF
HHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA