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The map label for this gene is hisS

Identifier: 49187294

GI number: 49187294

Start: 4209867

End: 4211138

Strand: Reverse

Name: hisS

Synonym: BAS4298

Alternate gene names: 49187294

Gene position: 4211138-4209867 (Counterclockwise)

Preceding gene: 49187295

Following gene: 49187293

Centisome position: 80.54

GC content: 40.09

Gene sequence:

>1272_bases
ATGTCTATTCAAATCCCACGCGGAACGCAAGATATTCTTCCAGGCACTGTTGAGTTATGGCAGTATATCGAAGGGCAAGC
ACGCGAAATTTGCCGTCGTTACAATTATAAAGAAATTCGTACACCGATCTTTGAACACACTGAGTTATTTTTACGTGGTG
TTGGTGATACGACAGATATCGTGCAAAAAGAAATGTACTCATTCCAAGATCGTGGAGAGCGTAGTTTAACATTACGTCCA
GAAGGCACTGCACCTGTTGTACGTTCTTACGTTGAAAACAAAATGTTCGGTGACGCAACACAACCAACGAAATTATATTA
TATCGGTCAAATGTTCCGTTATGAAAGACCACAAGCAGGTCGCTATCGTCAATTCGTACAATTCGGTATTGAAGCAATCG
GTAGTAACGATCCTGCAATTGATGCGGAAGTAATTGCACTTGCTGTAGAGTTTTACCGCGGCATGGGCTTAAAAAATATT
AAAGTTGTATTAAACAGCTTAGGTGATGCGGCGAGCCGTCAAGCGCACCGTGATGCGTTAATTGCACACTTTGAGCCACG
TATCGGTGAGTTCTGTTCTGACTGTCAATCTCGTTTAGAAAAGAACCCTCTTCGTATTTTAGATTGTAAGAAGGACCGTA
ACCATGAATTAATGGGAACAGCACCATCTATTACAGAATACTTAAACGAAGATTCAGCAGTATACTACGACAAAGTTCAA
GAACTATTAACGATGATGGATGTTCCATTTGAAAAAGATCCGAACTTAGTACGTGGTTTAGACTACTACCAGCACACTGT
TTTTGAAATTATGAGTGAAGCAGAAGGTTTCGGTGCGATCACTACACTAAGCGGTGGTGGCCGTTATAACGGACTTGTAC
AAGAAATCGGTGGACCAGAAATGCCAGGTATCGGTTTTGCGATGAGTATTGAACGTTTAATTATGGCGCTAAAAGCTGAA
AACATCGAATTACCAATTGAACATAGTATCGATTGTTACGTTGTAGCGCTTGGTGAAAAAGCGAAAGACCATGCTGCAAA
AGTTGCGTTTGATCTTCGTAAAGCTGGATTAGCAGTTGAAAAAGATTATTTAGATCGCAAAATGAAAGCACAATTTAAAT
CAGCAGATCGTCTAAAAGCGAAATTCGTAGCTGTACTAGGGGAAGATGAGTTAGATAAAGGCATCATTAACTTAAAAGAT
ATGGCAACAGGCGAACAAGAAGAAGTAGCATTAGATGTGTTTGCTTCATACGTAGCAGAGAAATTAATATAG

Upstream 100 bases:

>100_bases
GCACCACGGGTATAATACTCTCGTCCCTACTGTTCAAACAGTAGAGGCGGGAGTTTTTTTATTTTTATTAAGATTAAAAC
AATTTGAGGAGGAACTGAAT

Downstream 100 bases:

>100_bases
GGGGAACTTACAGTGGCTGAAAGAACACATGCATGTGGAAAAGTAACAGTAGAAGCAGTTGGACAAACAGTTCAATTAAA
AGGTTGGGTACAAAAACGCC

Product: histidyl-tRNA synthetase

Products: NA

Alternate protein names: Histidine--tRNA ligase 2; HisRS 2

Number of amino acids: Translated: 423; Mature: 422

Protein sequence:

>423_residues
MSIQIPRGTQDILPGTVELWQYIEGQAREICRRYNYKEIRTPIFEHTELFLRGVGDTTDIVQKEMYSFQDRGERSLTLRP
EGTAPVVRSYVENKMFGDATQPTKLYYIGQMFRYERPQAGRYRQFVQFGIEAIGSNDPAIDAEVIALAVEFYRGMGLKNI
KVVLNSLGDAASRQAHRDALIAHFEPRIGEFCSDCQSRLEKNPLRILDCKKDRNHELMGTAPSITEYLNEDSAVYYDKVQ
ELLTMMDVPFEKDPNLVRGLDYYQHTVFEIMSEAEGFGAITTLSGGGRYNGLVQEIGGPEMPGIGFAMSIERLIMALKAE
NIELPIEHSIDCYVVALGEKAKDHAAKVAFDLRKAGLAVEKDYLDRKMKAQFKSADRLKAKFVAVLGEDELDKGIINLKD
MATGEQEEVALDVFASYVAEKLI

Sequences:

>Translated_423_residues
MSIQIPRGTQDILPGTVELWQYIEGQAREICRRYNYKEIRTPIFEHTELFLRGVGDTTDIVQKEMYSFQDRGERSLTLRP
EGTAPVVRSYVENKMFGDATQPTKLYYIGQMFRYERPQAGRYRQFVQFGIEAIGSNDPAIDAEVIALAVEFYRGMGLKNI
KVVLNSLGDAASRQAHRDALIAHFEPRIGEFCSDCQSRLEKNPLRILDCKKDRNHELMGTAPSITEYLNEDSAVYYDKVQ
ELLTMMDVPFEKDPNLVRGLDYYQHTVFEIMSEAEGFGAITTLSGGGRYNGLVQEIGGPEMPGIGFAMSIERLIMALKAE
NIELPIEHSIDCYVVALGEKAKDHAAKVAFDLRKAGLAVEKDYLDRKMKAQFKSADRLKAKFVAVLGEDELDKGIINLKD
MATGEQEEVALDVFASYVAEKLI
>Mature_422_residues
SIQIPRGTQDILPGTVELWQYIEGQAREICRRYNYKEIRTPIFEHTELFLRGVGDTTDIVQKEMYSFQDRGERSLTLRPE
GTAPVVRSYVENKMFGDATQPTKLYYIGQMFRYERPQAGRYRQFVQFGIEAIGSNDPAIDAEVIALAVEFYRGMGLKNIK
VVLNSLGDAASRQAHRDALIAHFEPRIGEFCSDCQSRLEKNPLRILDCKKDRNHELMGTAPSITEYLNEDSAVYYDKVQE
LLTMMDVPFEKDPNLVRGLDYYQHTVFEIMSEAEGFGAITTLSGGGRYNGLVQEIGGPEMPGIGFAMSIERLIMALKAEN
IELPIEHSIDCYVVALGEKAKDHAAKVAFDLRKAGLAVEKDYLDRKMKAQFKSADRLKAKFVAVLGEDELDKGIINLKDM
ATGEQEEVALDVFASYVAEKLI

Specific function: Unknown

COG id: COG0124

COG function: function code J; Histidyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI15029520, Length=472, Percent_Identity=24.364406779661, Blast_Score=112, Evalue=8e-25,
Organism=Homo sapiens, GI6996014, Length=454, Percent_Identity=25.1101321585903, Blast_Score=106, Evalue=4e-23,
Organism=Escherichia coli, GI1788861, Length=420, Percent_Identity=41.6666666666667, Blast_Score=338, Evalue=5e-94,
Organism=Caenorhabditis elegans, GI71993693, Length=477, Percent_Identity=23.8993710691824, Blast_Score=102, Evalue=4e-22,
Organism=Caenorhabditis elegans, GI71993686, Length=477, Percent_Identity=23.8993710691824, Blast_Score=102, Evalue=5e-22,
Organism=Saccharomyces cerevisiae, GI6325290, Length=474, Percent_Identity=22.9957805907173, Blast_Score=98, Evalue=2e-21,
Organism=Drosophila melanogaster, GI24643061, Length=476, Percent_Identity=24.7899159663866, Blast_Score=119, Evalue=3e-27,
Organism=Drosophila melanogaster, GI24643059, Length=495, Percent_Identity=23.8383838383838, Blast_Score=108, Evalue=7e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): SYH2_BACAN (Q81LI6)

Other databases:

- EMBL:   AE016879
- EMBL:   AE017334
- EMBL:   AE017225
- RefSeq:   NP_846850.1
- RefSeq:   YP_021280.1
- RefSeq:   YP_030546.1
- ProteinModelPortal:   Q81LI6
- SMR:   Q81LI6
- EnsemblBacteria:   EBBACT00000013120
- EnsemblBacteria:   EBBACT00000017783
- EnsemblBacteria:   EBBACT00000019532
- GeneID:   1085478
- GeneID:   2814868
- GeneID:   2850534
- GenomeReviews:   AE016879_GR
- GenomeReviews:   AE017225_GR
- GenomeReviews:   AE017334_GR
- KEGG:   ban:BA_4633
- KEGG:   bar:GBAA_4633
- KEGG:   bat:BAS4298
- TIGR:   BA_4633
- TIGR:   GBAA_4633
- GeneTree:   EBGT00050000001169
- HOGENOM:   HBG616575
- OMA:   VFEWVTT
- ProtClustDB:   PRK00037
- BioCyc:   BANT260799:BAS4298-MONOMER
- BioCyc:   BANT261594:GBAA4633-MONOMER
- BRENDA:   6.1.1.21
- GO:   GO:0005737
- HAMAP:   MF_00127
- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR015807
- InterPro:   IPR004516
- Gene3D:   G3DSA:3.40.50.800
- PANTHER:   PTHR11476
- PIRSF:   PIRSF001549
- TIGRFAMs:   TIGR00442

Pfam domain/function: PF03129 HGTP_anticodon; PF00587 tRNA-synt_2b; SSF52954 Anticodon_bd

EC number: =6.1.1.21

Molecular weight: Translated: 47747; Mature: 47616

Theoretical pI: Translated: 4.94; Mature: 4.94

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSIQIPRGTQDILPGTVELWQYIEGQAREICRRYNYKEIRTPIFEHTELFLRGVGDTTDI
CEEECCCCCHHHCCCHHHHHHHHHHHHHHHHHHCCHHHHCCCHHHHHHHHHHCCCCHHHH
VQKEMYSFQDRGERSLTLRPEGTAPVVRSYVENKMFGDATQPTKLYYIGQMFRYERPQAG
HHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHHHHCCCCCCCCEEEEHHHHHHCCCCCCH
RYRQFVQFGIEAIGSNDPAIDAEVIALAVEFYRGMGLKNIKVVLNSLGDAASRQAHRDAL
HHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHCCHHHHHHHHHHH
IAHFEPRIGEFCSDCQSRLEKNPLRILDCKKDRNHELMGTAPSITEYLNEDSAVYYDKVQ
HHHCCHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCHHHHHHHCCCCCHHHHHHH
ELLTMMDVPFEKDPNLVRGLDYYQHTVFEIMSEAEGFGAITTLSGGGRYNGLVQEIGGPE
HHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCHHHHHCCCCC
MPGIGFAMSIERLIMALKAENIELPIEHSIDCYVVALGEKAKDHAAKVAFDLRKAGLAVE
CCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHCCHHH
KDYLDRKMKAQFKSADRLKAKFVAVLGEDELDKGIINLKDMATGEQEEVALDVFASYVAE
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEHHHHCCCCHHHHHHHHHHHHHHH
KLI
HCC
>Mature Secondary Structure 
SIQIPRGTQDILPGTVELWQYIEGQAREICRRYNYKEIRTPIFEHTELFLRGVGDTTDI
EEECCCCCHHHCCCHHHHHHHHHHHHHHHHHHCCHHHHCCCHHHHHHHHHHCCCCHHHH
VQKEMYSFQDRGERSLTLRPEGTAPVVRSYVENKMFGDATQPTKLYYIGQMFRYERPQAG
HHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHHHHCCCCCCCCEEEEHHHHHHCCCCCCH
RYRQFVQFGIEAIGSNDPAIDAEVIALAVEFYRGMGLKNIKVVLNSLGDAASRQAHRDAL
HHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHCCHHHHHHHHHHH
IAHFEPRIGEFCSDCQSRLEKNPLRILDCKKDRNHELMGTAPSITEYLNEDSAVYYDKVQ
HHHCCHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCHHHHHHHCCCCCHHHHHHH
ELLTMMDVPFEKDPNLVRGLDYYQHTVFEIMSEAEGFGAITTLSGGGRYNGLVQEIGGPE
HHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCHHHHHCCCCC
MPGIGFAMSIERLIMALKAENIELPIEHSIDCYVVALGEKAKDHAAKVAFDLRKAGLAVE
CCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHCCHHH
KDYLDRKMKAQFKSADRLKAKFVAVLGEDELDKGIINLKDMATGEQEEVALDVFASYVAE
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEHHHHCCCCHHHHHHHHHHHHHHH
KLI
HCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12721629