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The map label for this gene is greA

Identifier: 49187271

GI number: 49187271

Start: 4186187

End: 4186663

Strand: Reverse

Name: greA

Synonym: BAS4275

Alternate gene names: 49187271

Gene position: 4186663-4186187 (Counterclockwise)

Preceding gene: 49187272

Following gene: 49187270

Centisome position: 80.07

GC content: 38.78

Gene sequence:

>477_bases
ATGGCAACAGAAAAAACATACCCTATGACGCAAGAGGGTAAGCAAAAACTAGAGAACGAACTTGAGGATTTAAAAACGGT
AAAACGTAAAGAAGTTGTAGAGCGCATTAAGATTGCACGTAGCTTCGGAGATCTTTCTGAGAACTCTGAGTATGATGCAG
CGAAAGATGAGCAAGCGTTCGTAGAAGGACGTATTACACAATTAGAAAACATGATCCGTAACGCAGTTATCATCACAGAT
AATGGCGAAGAATCTACTGTTGTTACATTAGGTAAGACAGTAACATTTAAAGAATTACCAAATGGAGATGAAGAAGCTTA
CACAATCGTAGGTAGTGCGGAAGCAGACCCATTTGAAGGAAGAATTTCTAACGATTCTCCAATCGCGAAAAGCTTATTAG
GTAAGCAAATTGGTGAGAAGGTAGCAATCCAAACACCAGGTGGAGAAATGCAAGTAGAGATTATCTCTGTAAAATAA

Upstream 100 bases:

>100_bases
CAGGCGTGCTAGAAACCTCCGCTCTCTATATAAGCGGGGGAGTTTTCATATGGAACTCCTCTTTTTTTCGGGGGATTGGT
ATATAAAAGGAGTGGAAAAT

Downstream 100 bases:

>100_bases
TTAAAAATCACTCGTGTAAAAGCGAGTGATTTTTTTATTTGTATAAAAGATGAAACACTCCGTCCAAACTATAATAGACG
AGGTGTTTTTTTATGAAAAT

Product: transcription elongation factor GreA

Products: NA

Alternate protein names: Transcript cleavage factor greA

Number of amino acids: Translated: 158; Mature: 157

Protein sequence:

>158_residues
MATEKTYPMTQEGKQKLENELEDLKTVKRKEVVERIKIARSFGDLSENSEYDAAKDEQAFVEGRITQLENMIRNAVIITD
NGEESTVVTLGKTVTFKELPNGDEEAYTIVGSAEADPFEGRISNDSPIAKSLLGKQIGEKVAIQTPGGEMQVEIISVK

Sequences:

>Translated_158_residues
MATEKTYPMTQEGKQKLENELEDLKTVKRKEVVERIKIARSFGDLSENSEYDAAKDEQAFVEGRITQLENMIRNAVIITD
NGEESTVVTLGKTVTFKELPNGDEEAYTIVGSAEADPFEGRISNDSPIAKSLLGKQIGEKVAIQTPGGEMQVEIISVK
>Mature_157_residues
ATEKTYPMTQEGKQKLENELEDLKTVKRKEVVERIKIARSFGDLSENSEYDAAKDEQAFVEGRITQLENMIRNAVIITDN
GEESTVVTLGKTVTFKELPNGDEEAYTIVGSAEADPFEGRISNDSPIAKSLLGKQIGEKVAIQTPGGEMQVEIISVK

Specific function: Necessary for efficient RNA polymerase transcription elongation past template-encoded arresting sites. The arresting sites in DNA have the property of trapping a certain fraction of elongating RNA polymerases that pass through, resulting in locked ternary

COG id: COG0782

COG function: function code K; Transcription elongation factor

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the greA/greB family

Homologues:

Organism=Escherichia coli, GI87082225, Length=158, Percent_Identity=44.3037974683544, Blast_Score=122, Evalue=9e-30,
Organism=Escherichia coli, GI87082261, Length=145, Percent_Identity=33.7931034482759, Blast_Score=74, Evalue=6e-15,

Paralogues:

None

Copy number: 259 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): GREA_BACAA (C3P968)

Other databases:

- EMBL:   CP001598
- RefSeq:   YP_002868667.1
- ProteinModelPortal:   C3P968
- SMR:   C3P968
- EnsemblBacteria:   EBBACT00000125393
- GeneID:   7852008
- GenomeReviews:   CP001598_GR
- KEGG:   bai:BAA_4627
- GeneTree:   EBGT00050000001871
- ProtClustDB:   PRK00226
- GO:   GO:0006350
- HAMAP:   MF_00105
- InterPro:   IPR018151
- InterPro:   IPR006359
- InterPro:   IPR001437
- InterPro:   IPR022691
- Gene3D:   G3DSA:1.10.287.180
- Gene3D:   G3DSA:3.10.50.30
- TIGRFAMs:   TIGR01462

Pfam domain/function: PF01272 GreA_GreB; PF03449 GreA_GreB_N; SSF46557 GreA_GreB

EC number: NA

Molecular weight: Translated: 17438; Mature: 17307

Theoretical pI: Translated: 4.36; Mature: 4.36

Prosite motif: PS00829 GREAB_1; PS00830 GREAB_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATEKTYPMTQEGKQKLENELEDLKTVKRKEVVERIKIARSFGDLSENSEYDAAKDEQAF
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHH
VEGRITQLENMIRNAVIITDNGEESTVVTLGKTVTFKELPNGDEEAYTIVGSAEADPFEG
HHHHHHHHHHHHHCEEEEECCCCCCEEEEECCEEEECCCCCCCCCEEEEEECCCCCCCCC
RISNDSPIAKSLLGKQIGEKVAIQTPGGEMQVEIISVK
CCCCCCHHHHHHHHHHHCCEEEEECCCCCEEEEEEEEC
>Mature Secondary Structure 
ATEKTYPMTQEGKQKLENELEDLKTVKRKEVVERIKIARSFGDLSENSEYDAAKDEQAF
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHH
VEGRITQLENMIRNAVIITDNGEESTVVTLGKTVTFKELPNGDEEAYTIVGSAEADPFEG
HHHHHHHHHHHHHCEEEEECCCCCCEEEEECCEEEECCCCCCCCCEEEEEECCCCCCCCC
RISNDSPIAKSLLGKQIGEKVAIQTPGGEMQVEIISVK
CCCCCCHHHHHHHHHHHCCEEEEECCCCCEEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA